The Pattern of Expression of Phospholipase C-γ1 in Benign and Malignant Tumors of the Breast

Hee Joung Kim(김희정),Ki-Wook Chung(정기욱),Sung-Won Kim(김성원),Byung In Moon(문병인),Soo-Jung Ahn(안수정),Dong-Young Noh(노동영),Keun-Young Yoo(유근영),Kuk Jin Choe(최국진) 대한외과학회 2002 Annals of Surgical Treatment and Research Vol.62 No.6

Identification of Differentially Expressed Genes in Bovine Follicular Cystic Ovaries

Choe, Chang-Yong,Cho, Young-Woo,Kim, Chang-Woon,Son, Dong-Soo,Han, Jae-Hee,Kang, Da-Won The Korean Society of Pharmacology 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.5

Follicular cystic ovary (FCO) is one of the most frequently diagnosed ovarian diseases and is a major cause of reproductive failure in mammalian species. However, the mechanism by which FCO is induced remains unclear. Genetic alterations which affect the functioning of many kinds of cells and/or tissues could be present in cystic ovaries. In this study, we performed a comparison analysis of gene expression in order to identify new molecules useful in discrimination of bovine FCO with follicular cystic follicles (FCFs). Normal follicles and FCFs were classified based on their sizes (5 to 10 mm and $\geq25mm$). These follicles had granulosa cell layer and theca interna and the hormone $17{\beta}$-estradiol ($E_2$)/ progesterone ($P_4$) ratio in follicles was greater than one. Perifollicular regions including follicles were used for the preparation of RNA or protein. Differentially expressed genes (DEG) that showed greater than a 2-fold change in expression were screened by the annealing control primer (ACP)-based PCR method using $GeneFishing^{TM}$ DEG kits in bovine normal follicles and FCFs. We identified two DEGs in the FCFs: ribosomal protein L15 (RPL15) and microtubule-associated protein 1B (MAP1B) based on BLAST searches of the NCBI GenBank. Consistent with the ACP analysis, semi-quantitative PCR data and Western blot analyses revealed an up-regulation of RPL15 and a down-regulation of MAP1B in FCFs. These results suggest that RPL15 and MAP1B may be involved in the regulation of pathological processes in bovine FCOs and may help to establish a bovine gene data-base for the discrimination of FCOs from normal ovaries.

Immunofluorescent Study on Localization of Bordetella Pertussis Antigen in the Central Nervous System of Mice After Pertussis Vaccine Injection

Choe, Jin,Kim, Won Il,Chun, Chong Hwee,Chung, Hee Young CATHOLIC MEDICAL CENTER 1973 Bulletin of the Clinical Research Institute Vol.1 No.1

Travelling route of B. pertussis antigen into the central nervous system after the intravenous, intraneural and perineural. vaccine injection were examined with immuno- fluorescent antibody technique. B. pertussis antigen were detected in the central nervous system after 24 hours following the intravenous vaccine injection. But not detected until 12 hours after intravenous injection. And it is evident that B. pertussis vaccine can reach to the central nervous system widely through hematogenous route by permeating the b1ood-brain barrier but not by way of peripheral nerve route. The above findings explain the possible pathogenesis of encephalitis and convulsion occurred in the pertussis vaccinated people.

Gadolinium-Enhancement on Fast Spin-Echo MR Imaging : Impact on Diagnosis of Meniscal Tears and Differentiation of Acute and Chronic Ligament Tears

Choe, Bo-Young,Shinn, Kyung-Sub,choi, Kyu-Ho,Jee, Won-Hee,Kim, Jung Man 가톨릭 의과학연구원 1997 가톨릭 의과학연구원 국제학술대회 Vol.1 No.-

In conclusion, our present results showed that FSE pulse sequence could be acceptable in the diagnosis of meniscal tears, and additional FSGE MR imaging could enhance meniscal detectability (i. e., 3% increase in our study). Also, FSGE MR imaging could be useful in differentiating acute from chronic ligament injury.

A case of epithelioid angiosarcoma of the lungs presenting as pleural and pericardial effusion (초)

( Won Seok Lee ),( Hee Moon ),( Yeong Hun Choe ),( So Ri Kim ),( Heung Bum Lee ),( Yong Chul Lee ),( Yang Keun Rhee ),( Seoung Ju Park ) 대한내과학회 2008 대한내과학회 추계학술대회 Vol.2008 No.-

Acute hemorrhagic edema of young children: Erythema multiforme-like vasculitis

( Hee Chul Chung ),( Sung Jay Choe ),( Solam Lee ),( Won Soo Lee ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.1

Vasculitis is characterized by two main features, including violaceous palpable purpura or petechiae, both of which are most likely to appear on the lower extremities. Erythema multiforme (EM) has target-like lesions, which appear as concentric circles with different contrasts. Acute hemorrhagic edema (AHE) of young children is uncommon, but when the condition is present, it is typically underestimated as cutaneous leukocytoclastic vasculitis (LCV). AHE becomes diagnostic when both EM-like lesions and violaceous palpable purpura exist. The four-year-old patient had palpable purpura and targetoid manifestations localized on the lower extremities, and based on biopsy, the diagnosis was direct immunofluorescent staining-negative leukocytoclastic vasculitis. There was a history of upper respiratory infection and an influenza vaccination occurring three weeks before onset of the condition, suggesting that an infectious causative origin might have brought about acute hemorrhagic edema (AHE). Oral Prednisolone with a dosage of 10 mg/day was started, and the dose was tapered 2.5 mg/week. Four weeks of systemic steroids successfully controlled the lesions. Because of the targetoid palpable purpura, AHE tends to be underestimated or misdiagnosed as other diseases in pediatric patients. If the patient presents with symptoms that are not conclusively one of the two conditions, then AHE should be taken into consideration.

A case of epithelioid angiosarcoma of the Lungs presenting as pleural and pericardial effusion (초)

( Won Seok Lee ),( Hee Moon ),( Yeong Hun Choe ),( So Ri Kim ),( Heung Bum Lee ),( Yong Chul Lee ),( Yang Keun Rhee ),( Seoung Ju Park ) 대한내과학회 2008 대한내과학회지 Vol.75 No.1S

Vulvar Bowen`s disease: the importance of dermatologic diagnosis

( Hee Chul Chung ),( Sung Jay Choe ),( Won Soo Lee ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.1

A 62-year-old female presented a well-demarcatederythematous painful patch with mild erosion on the vulva and perineum for 3 years. Although she had been treated by antifungal and antiviral therapy for several years at local gynecologic and urologic clinics, lesions were not improved. Histopathologically, the specimen revealed full-thickness epidermal proliferation with disorder and wind-blown appearance, while the dermis remained intact. Human papilloma virus type 16 was detected on the lesion. Along with above findings, diagnosis of vulva Bowen’s disease was established, followed by total excision of the lesion. Bowen’s disease on the vulvar area, so called vulvar intraepithelial neoplasia, could occasionally show atypical presentations such as erosion, ulceration and hyperpigmentation. In addition, not so much cases of vulva Bowen’s disease were reported, considering there have been only four cases of female genital Bowen’s disease in department of dermatology of our hospital from 2001 to 2014. However, dermatologists should pay more attention to the genital skin disease such as vulva Bowen’s disease than other physcians. Therefore, we recommend that the biopsy should be taken into consideration when atypical genital lesions were encountered, because many cases of vulvar Bowen’s disease would be undiscovered unexpectedly.

Maize with Multiple Ears and and Tillers(MET) IV. Leaf Characteristics of IK Type Maize with Tillers

Choe, Bong-Ho,Lee, Hee-Bong,Lee, Won-Koo,Kang, Kwon-Kyu,Choi, Chang-Yeol The Korean Society of Crop Science 1989 Korean journal of crop science Vol.34 No.4

분얼하는 옥수수 교잡종 (IK//1R1/B68)의 잎이 분얼하지 않는 옥수수(진주옥)의 잎과 길이나, 폭 또는 면적에 있어서 차이가 있는지의 여부를 알기위해 주경 또는 분얼경의 지엽으로 부터 아래로 9개 잎을 수확기에 취하여 엽장, 엽폭, 엽면적을 측정하였다. 1. 개체당 주경의 평균 엽장은 IK형 (IK//IRI/B68)이나 진주옥이 거의 같았으나 평균 엽폭은 IK형이 진주옥보다 약 2cm 좁았다. 평균 엽면적 역시 IK형이 적었는데 그 이유는 엽폭이 좁았기 때문이었다. 2. IK형 옥수수에서 주경과 분엽경간의 잎특성(장, 폭. 면적)은 서로 비슷하였다. 3. IK형 옥수수는 착수절 바로 아래 절의 잎이 가장 길었고 착수절 바로 위의 잎이 가장 넓었다. 4. IK형 옥수수 잎의 절입별 병이계수를 비교한 결과 착수절을 포함한 착수절 근처 잎이 변이계수가 가장 낮았고, 착수절에서 멀리 떨어진 잎일수록 변이계수의 값이 컸다. 특히 정엽의 변이계수는 다른 잎들보다 훨씬 컸다. 이같은 경향은 주경과 분얼경의 잎이 모두 같았다. 5. 기존의 공식(장$\times$폭$\times$0.75)에 의해 추정한 엽면적보다는 직접 측정한 엽면적이 컸다. 따라서 IK형 옥수수의 엽장과 엽폭에 의한 엽면적 추정은 기존의 0.75 대신에 0.80을 이용하는 것이 보다 타당할 것으로 생각된다. Leaf characteristics of main stem and tiller of IK type maize (IK/ /IRI/B68) were compared with those of Jinjoo Ok hybrid which are not usually tillered. A total of nine leaves from flag leaf to the third or the fourth leaf below ear-bearing node were sampled from each stem or tiller. There was no significant difference in mean leaf length between IK/ /IRI/B68 and Jinjoo Ok. But the mean leaf width of IK/ /IRI/ B68 was about 2 cm narrower than that of Jinjoo Ok. The mean leaf area of the IK/ /IRI/B68 was also smaller than that of Jinjoo Ok due to the narrower leaf width. There were not significant differences in mean leaf characteristics between main stem and tillers of IK/IRI/B68. The longest leaf was the leaf below the ear-bearing node and the widest leaf was the leaf just above the ear-bearing node. Mean length. width and area of leaf on main stem and tillers were similar. Coefficients of variation calculated for individual leaf indicated that the leaves near the ear-bearing node were more uniform than others. The leaf area measured was significantly greater than that estimated by formular, length x width x 0.75. New constant to estimate leaf area of tillering maize was derived as 0.8.

The Maize with Multiple Ears and Tillers (MET) III. Developmental Habit and Morphology of the Tillers

Choe, Bong-Bo,Lee, Hee-Bong,Lee, Won-Koo,Kang, Kwon-Kyoo,Jong, Seung-Keun The Korean Society of Crop Science 1989 Korean journal of crop science Vol.34 No.1

분얼하는 옥수수에서 분얼의 발생시기 발생부위, 개체당분얼수, 분얼각, 근계, 크기등에 대해 조사 보고된 바가 없기에 이들 특성을 알기 위해 국내 재래종에서 순계분리한 자식계통 및 교잡종을 중심으로 1987년과 2988년에 포장조사한 것을 다음과 같이 요약한다. 1. 일차분 시기는 충남의 유성에서 5월 11일경 파종시 주경의 제일본엽이 지하에 출현후 18일 내지 20일이었고, 제일차분얼은 일차분얼이 있은 후 4~5일 후였다. 자식계통들 사이와 교잡종들 사이에는 분얼의 발생시기에 큰 차이가 없었고 교잡종 자식계통보다 2일 정도 빨랐다. 2. 분얼들의 발생부위는 거의 모든 시험계통들이 주경의 지하절에서 였다.(예외 : Tlr 계통) 3. 개체당 분얼수는 재식밀도에 관계없이 계통 및 교잡종에 따라 달랐다. IK나 IRI같은 자식계토은 개체당 분얼수가 2~3개로 정해졌으나 왜성과 같은 계통은 5내지 10개가 되기도 하였다. (IK$\times$IRI)F$_1$은 3~4개였다. 4. 주경과 분얼이 가지는 분얼각도 유전적 특성으로 대략 3개로 구분할 수 있었다. 1군은 분얼각이 없거나 매우 적은 것, 2군은 분얼각이 45$^{\circ}$정도 되는 것, 3군은 분얼각이 45$^{\circ}$이하되는 것들이다. 5. 분얼의 크기(길이, 직경)는 유효경 여부를 결정하는데 중요한데 대부분 재래종 계통이나 이들로 만들어진 교잡종은 60cm$\times$30cm의 재식밀도하에서 유효경 비율이 90% 이상되었다. 6. 분얼의 크기(길이, 직경)는 유효 경 여부를 결정하는데 중요한데 대부분 재래종 계통이나 이들로 만들어진 교잡종은 60cm$\times$30cm의 재식밀도하에서 유효경 비율이 90% 이상되었다.^{\circ}C$처리기간중에는 근의 부위에 관계없이 Rf치가 낮은 쪽에서는 증가하였으나 높은 쪽에서는 상대적으로 감소하였다. 4. 이상의 결과를 종합하여 보면 인삼근에 있어서 신아의 휴면타파 및 맹아는 ABA와는 별로 관계가 없고 온도숙건에 지배되어 나타나는 GA의 생합성 능력과 그 생합성에 의한 활성증가와 밀접한 관계가 있는 것으로 추정된다.는 0.2045, 0.1208, 0.2248로 추정되었다. 산란율과 난중과의 관계는 0.6233, -0.2259, 0.2973, 500일영 체중과는 0.2417, 0.0774, - 0.4787로 추정되었다. 산란지수와 난중과의 관계는 0.6171, -0.2706, 0.4579, 500일영 체중과의 관계 0.3082, -0.0792, -0.3368로 추정하였다. 난중과 500일영 체중과의 관계는 0.2742, 0.2205, 0.1354로 추정되었다.)에서 47.4%의 가장 높은 검색률을 나타내었고 다음 성계(150일영이상)에서 35.3% 및 유추(30 일영미만)에서 17.3%의 순이었다. 이중 세균성 및 기생충성 질병은 육성계에서의 검색률이 가장 높았으나, 바이러스성 질병은 육성계 및 성계에서 거의 같은 비율로 높았으며 곰팡이성 질병은 유추에서 가장 높은 검색률을 나타내었다.17) 사료비절감은 생산비의 60∼70%를 차지하고 있으므로 공동구인 자가배합을 하여 자기실정에 맞는 경영체질로 운영하고 있었다. (18) 인건비가 생산비의 15∼20%의 비율이며 또한 사람 구득난이라 가족노동을 최대한 활용, 관리를 하고 있으며 생력화를 위해 기계화, 자동화되어 가고 있었다. 애정과 정성심있는 관리로 닭을 스트레스를 주지 않게 하기 위해 가족관이, 기계자동화가 중요한 역할을 하고 있었다. (19) 시설기구와 육성상각비를 줄이기 위해 관리자의 변동이 없고, 종신직장으로서 긍지와 In order to investigate developmental habit and morphology of maize tillers, time and location of tiller development. number of tillers per plant, tiller angle, height and diameter of tillers and root systems of tillers were examined under field condition for maize with tillers. Materials used were mostly from Korean local lines and a few lines from other countries were also included for comparison. The time of the first tiller development was about 18 to 20 days after emergence when planted on May in Yusong. The second tiller appeared about 4 to 5 days after the first tiller appeared. The tiller number per plant varied with lines and hybrids and ranged from two to ten. The location of tiller development was usually basal nodes of the main stem. Each tiller appeared to have its own root system. The angle between tillers and main stem was variable depending upon the maizes and the tiller angle could be classified into three categories. The height of tillers was also variable and seemed to be under genetic control. The most productive tillers were found among the Korean local derivatives.