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- 저자 : Chen Wenjia (검색결과 3 건)
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Chen Wenjia,Ma Zhaochen,Yu Lingxiang,MAO Xia,Ma Nan,Guo Xiaodong,Yin Xiaoli,Jiang Funeng,Wang Qian,Wang Jigang,Fang Mingliang,Lin Na,Zhang Yanqiong 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Artesunate (ART) has been indicated as a candidate drug for hepatocellular carcinoma (HCC). Glucosylceramidase (GBA) is required for autophagic degradation. Whether ART regulates autophagic flux by targeting GBA in HCC remains to be defined. Herein, our data demonstrated that the dramatic overexpression of GBA was significantly associated with aggressive progression and short overall survival times in HCC. Subsequent experiments revealed an association between autophagic activity and GBA expression in clinical HCC samples, tumor tissues from a rat model of inflammation-induced HCC and an orthotopic mouse model, and human HCC cell lines. Interestingly, probe labeling identified GBA as an ART target, which was further verified by both a glutathione-S-transferase pulldown assay and surface plasmon resonance analysis. The elevated protein expression of LC3B, the increased numbers of GFP-LC3B puncta and double-membrane vacuoles, and the enhanced expression of SQSTM1/p62 indicated that the degradation of autophagosomes in HCC cells was inhibited by ART treatment. Both the in vitro and in vivo data revealed that autophagosome accumulation through targeting of GBA was responsible for the anti-HCC effects of ART. In summary, this preclinical study identified GBA as one of the direct targets of ART, which may have promising potential to inhibit lysosomal autophagy for HCC therapy.
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Function of Rhs proteins in porcine extraintestinal pathogenic Escherichia coli PCN033
Lu Wenjia,Tan Jia,Lu Hao,Wang Gaoyan,Dong Wenqi,Wang Chenchen,Li Xiaodan,Tan Chen 한국미생물학회 2021 The journal of microbiology Vol.59 No.9
Extraintestinal pathogenic Escherichia coli (ExPEC) is an important zoonotic pathogen that places severe burdens on public health and animal husbandry. There are many pathogenic factors in E. coli. The type VI secretion system (T6SS) is a nano-microbial weapon that can assemble quickly and inject toxic effectors into recipient cells when danger is encountered. T6SSs are encoded in the genomes of approximately 25% of sequenced Gram-negative bacteria. When these bacteria come into contact with eukaryotic cells or prokaryotic microbes, the T6SS assembles and secretes associated effectors. In the porcine ExPEC strain PCN033, we identified four classic rearrangement hotspot (Rhs) genes. We determined the functions of the four Rhs proteins through mutant construction and protein expression. Animal infection experiments showed that the Δrhs-1CT, Δrhs-2CT, Δrhs-3CT, and Δrhs-4CT caused a significant decrease in the multiplication ability of PCN033 in vivo. Cell infection experiments showed that the Rhs protein is involved in anti-phagocytosis activities and bacterial adhesion and invasion abilities. The results of this study demonstrated that rhs1, rhs3, and rh4 plays an important role in the interaction between PCN033 and host cell. Rhs2 has contribution to cell and mice infection. This study helps to elucidate the pathogenic mechanism governing PCN033 and may help to establish a foundation for further research seeking to identify potential T6SS effectors.
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Evolution of Mechanical Properties of Ti–6Al–4V Alloy in the Temperature Range of 20 to − 196 °C
Wei Zhao,Wenjia Su,Liang Li,Ding Fang,Ni Chen 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.9
Cryogenic cooling is becoming an attractive method for machining difficult-to-cut materials to extend their machinability. However, it’s a challenging task to analyze the effect of cryogenic cooling on the machining. In order to better understandthe various physical phenomena in the cryogenic cutting process, cryogenic impact test, and tensile test of titanium alloyTi–6Al–4V were undertaken in this work. The impact and tensile properties of Ti–6Al–4V alloy in the temperature range of20 to − 196 °C were investigated. Later on, the macroscopic and microscopic morphology of the produced fractures wereanalyzed. Findings have depicted a decreasing impact toughness and an increase of tensile and yield strength of Ti–6Al–4Valloy in the temperature range from 20 to − 196 °C. The impact fracture appeared as a tendency of becoming material brittle. Meanwhile, three kinds of zones of shear-lip, fiber, and radiation were found on the fracture morphology. Those zonescorrespond to the shear fracture zone, crack initiation zone, and crack extension zone, respectively. With the temperaturedecrease, the proportion of fiber zone was decreased, while a gradual increase in the radiation zone was observed. Besides,the fiber zone was observed for the macroscopic morphology of the fracture under the temperature of − 196 °C. Somedimples were found in the tensile fracture at − 196 °C indicating the ability of Ti–6Al–4V alloy to deform plastically undersuch low temperature. This work is a fundamental support to analyze the behavior of Ti–6Al–4V alloy under cryogeniccooling conditions.
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