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- 저자 : Chen Rongchun (검색결과 3 건)
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Chen Qin,Wu Yaohong,Zhong Mingliang,Xu Chanhua,Chen Rongchun,Liu Ning 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.2
Background Intervertebral disk degeneration (IDD) is a common orthopedic disorder, and nucleus pulposus cells (NPCs) serve to stabilize the intervertebral disk. Objective This study was implemented to explore the protective eff ect of endplate stem cells (EPSCs) on IDD. NPCs and EPSCs were isolated from rats and tert -Butyl peroxide (TBHP) was used to treat NPCs and simulate IDD. EPSC-derived conditioned medium (CM) was collected and mixed with a culture medium at diff erent proportions, which was then used to treat NPCs. SOD assay was employed to examine the oxidative stress level in the NPCs. To assess apoptosis and caspase-3 activity, fl ow cytometry and western blots for cleaved PARP1 and BAX were carried out. The mRNA expression levels of diff erent genes were detected by quantitative real-time polymerase chain reaction. Furthermore, the concentrations of amphiregulin (Areg) and infl ammatory factors were measured with ELISA kits and cell signaling pathways were evaluated using western blotting. Results Following TBHP treatment, caspase-3 activity in the NPCs increased. However, exposing TBHP-induced NPCs to the EPSC-derived CM reduced oxidative stress, caspase-3 activity, cell apoptosis, fi brotic response, and cytokine production. Additionally, the EPSC-derived CM contained a higher concentration of Areg compared to that in the NPCs. Furthermore, Areg decreased oxidative stress, cell apoptosis, fi brosis, and infl ammation via both PI3K/AKT and ERK1/2 signaling pathways. Conclusion ECSCs reduce NPC apoptosis, oxidative stress, production of infl ammatory factors, and fi brotic response via the release of Areg. Moreover, Areg can exert these protective eff ects on NPCs via PI3K/AKT and ERK1/2 signaling pathways.
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Zhilei Miao,Wei Wang,Rongchun Yuan,Jun Zhu,Shudong Wu,Haitao Chen,Xianghua Zeng,Qiang Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.09
We have fabricated BiFeO3 thin film deposited on Pt/Ti/SiO2/Si substrates by the chemical solution deposition method. The effects of annealing temperature on the thin film structure, resistance switching (RS) properties, conduction mechanisms are investigated. It exhibits improved RS window with high ON/OFF ratio ( ~10 4) for the sample annealed at 650 ℃. XPS characterization indicates that cation ratio of Fe2+/Fe3+ is increased with increasing annealing temperature. Crystal lattice distortion generated by Fe2+ cations, along with oxygen vacancies, commonly contribute to opening the RS window and the increment of conductive filaments. The film's conduction mechanisms under different annealing temperatures are fully discussed. The RS properties of this system can be effectively improved by increasing the annealing temperature, which is crucial prerequisite for future applications of BFO-based thin film device in resistance random access memory.
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CO hydrogenation to higher alcohols over Ni- and Mo-modified Cu/CeO2 catalyst
Wei Li,Jun Chen,Rongchun Shen 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.2
Ni-Mo promoted Cu/CeO2 catalyst was synthesized by co-precipitation method using 28%wt NH3·H2O as the precipitant. The catalysts were characterized by BET, XRD, TPR and XPS. The results showed that Ni and Mo could promote the reducibility of Cu, and the interaction between Ni and Mo may be needed for catalytic activity and higher alcohols synthesis. Therefore, CuNiMo/CeO2 catalyst showed a higher activity for higher alcohols synthesis than Cu/CeO2. In the meantime, the effect of pyrogallol used in preparing the CuNiMo/CeO2 catalyst was investigated. Pyrogallol had a significant influence on lowering the methanol selectivity and improving the C2+-OH selectivity. The methanol selectivity decreased from 52.99% to 44.81% and SC2+OH/SMeOH (MeOH denoted as methanol) ratio increased from 0.27 to 0.35. The XPS results gave evidence that pyrogallol can form complexes with Cu+ on the CeO2 support, which causes methanol decrease. In addition, pyrogallol could serve as a “temperature addictive agent” to save power.
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