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Effect of Temperature on the Killing of Opisthorchis viverrini Eggs In Vitro
Parichart Boueroy,Kunyarat Duenngai,Chatanun Eamudomkarn,Panupan Sripan,Thidarut Boonmars,Benjamabhorn Pumhirunroj,Atchara Artchayasawat,Jiraporn Songsri,Kanpicha Chomphumee,Panaratana Rattanasuwan,Po 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.1
Contaminated liver fluke egg in the environment has led to the high prevalence of human opisthorchiasis asso- ciated with cholangiocarcinoma in Southeast Asia. To find the effective lessening methods of Opisthorchis viverrini eggs in the contaminated environment, we investigated the temperature conditions for killing of these trematode eggs in vitro. Numerous O. viverrini eggs were obtained in the proximal part of uteri of adult worms from experimental hamsters. Ma- ture eggs with miracidium were allocated by experimental groups (2 control: positive and negative and 4 treatment: 50, 60, 70, and 80°C) with 0.85% saline, and treated by the experimental plan. Eggs in each experimental groups were ob- served under the confocal microscope after stain with Propidium Iodide (PI) to evaluate the effect of temperatures. Eggs in 70 and 80°C groups were all killed after over 10 min heated. Majority of eggs in 60°C (10, 15, and 30 min heated), 70 and 80°C (5 min heated) groups were inactivated. However in 50°C group, below half of eggs were to be killed in all time lapse (10, 15 and 30 min). In order to prevent O. viverrini infection and cholangiocarcinoma, direct treatment of sewage by heating at 70 or 80°C at least 10 min is essential. Therefore, treatment of O. viverrini eggs at a high temperature is a po- tential method for controlling egg contamination in sewage.
Mitochondrial Genome of Spirometra theileri Compared with Other Spirometra Species
Barakaeli Abdieli Ndosi,Hansol Park,Dongmin Lee,Seongjun Choe,Yeseul Kang,Tilak Chandra Nath,Mohammed Mebarek Bia,Chatanun Eamudomkarn,Hyeong-Kyu Jeon,Keeseon S. Eom 대한기생충학열대의학회 2021 The Korean Journal of Parasitology Vol.59 No.2
최성준,김성룡,나기정,Tilak Chandra Nath,Barakaeli Abdieli Ndosi,강예슬,BIA MOHAMMED MEBAREK,이동민,박한솔,Chatanun Eamudomkarn,전형규,엄기선 대한기생충학ㆍ열대의학회 2020 The Korean Journal of Parasitology Vol.58 No.3
In November 2019 a 5-month-old mixed-breed rabbit presented to Chungbuk National University Veterinary Teaching Hospital, Cheongju-si, Chungbuk, Republic of Korea (Korea) with symptoms comprising pruritus, crusts on skin, poor appetite and reduced defecation. The rabbit was purchased 2 months prior from a pet shop located in a big market, and that the symptoms were first observed about 2 weeks prior to the hospital visit. Physical examination revealed that the patient had crust formation and alopecia on the nose together with lesions on the digits. A skin scraping test was per- formed using mineral oil and a high density of mites was observed by microscopy. Each mite showed a round, tortoise- like body with 4 comparatively short pairs of legs. The anus was located at the terminal unlike with suspected pathogen, Notoedres cati. Based on morphological characteristics, we identified the mite as Sarcoptes sp. Ivermectin was adminis- tered weekly by subcutaneous injection at a dosage of 0.4 mg/kg, and 4 weeks of follow-up study revealed the patient was fully recovered. And no more mites were detected from the case. This is the first case report of sarcoptic mange in a pet rabbit in Korea.