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- 저자 : Chaoyin Chen (검색결과 5 건)
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Wanxing Wu,Shenglan Zhao,Chaoyin Chen,Feng Ge,Diqiu Liu,Xiaoming He 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.6
Solid-state fermentation (SSF) of walnut proteinmeal (WPM) by Bacillus subtilis was optimized for themaximum degree of hydrolysis (DH) and reducing power(RP) using response surface methodology (RSM). Optimumfermentation conditions were fermentation time of 82.01 h,inoculum concentration of 10.40, and water content of 1.50mL/g. Optimized values were 41.80% and 0.78 for DH andRP, respectively. Walnut peptides (WP) were ultrafiltrationmembrane fractionated. The WPs-II (molecular weight <5kDa) fraction showed the highest RP value. WPs-II showedgood DPPH free radical scavenging and Fe2+ chelatingactivities, higher than for GSH (L-glutathione reduced) atthe same concentration. Amino acid composition analysisof WPs-II showed that Asp, Glu, and Arg were the majoramino acids playing important roles in the antioxidantactivity. WPs is an efficient antioxidant suitable for use asa food additive and as a pharmaceutical agent.
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Quantitative Indirect ELISA for Determination of Walnut Proteins in Foods
Juan Fang,Dan Chen,Chaoyin Chen,Feng Ge,Diqiu Liu,Benyong Han,Xiangfeng Xiong 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6
An indirect enzyme-linked immunosorbent assay (ELISA) capable of detecting walnut proteins in commercial products was developed. Polyclonal antibodies against soluble walnut protein were used. Specific epitopes of walnut protein were analyzed for cross activity with peanut, soybean, sesame, rice, wheat, jujube, and defatted milk protein. Epitopes of walnut proteins had no cross activity. The indirect ELISA was highly specific for soluble walnut proteins. Recovery values from walnut protein solutions ranged from 88.47 to 115%, and low coefficients of variation (<10%) indicated good repeatability. Intra and inter-assay precisions were <6 and <7 %, respectively. The limit of detection was 10 ng/mL of soluble walnut protein. The method was applied to detect some commercial food products in China and the results showed that indirect ELISA could be promisingly used to quantify adulteration of walnut processed foods.
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Research on the kinematic calibration of the 3-PTT parallel mechanism
Liang'en Huang,Minfang Chen,Shigao Zheng,Chaoyin He,Enxiao Zhu,Yongxia Zhang 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.8
Most of the kinematic calibration methods of parallel mechanisms only consider the geometric error of the mechanism, and the calibration effect is poor. To improve the calibration effect, this paper takes the 3-PTT (3-prismatic hook hook) parallel mechanism as the research object and proposes a kinematics calibration method based on the normalized representation model of the geometric error and non-geometric error of the mechanism. First, the degree of freedom of the mechanism is analyzed, its kinematics positive and negative solutions are solved, and its singularity is analyzed. Secondly, a normalized characterization method of mechanism geometric errors and non-geometric errors is proposed, and an error model without redundant parameters is constructed. The end motion error of the machine is measured by laser tracker, and the objective function is constructed. The genetic algorithm is designed to solve the minimum value of the objective function, and the normalized error of the mechanism is identified. By comparing with the recent methods, the better identification performance of the algorithm in this paper is verified. Finally, the kinematics model was corrected according to the identification results. After the calibration was completed, the movement errors of the end of the mechanism along each coordinate axis were reduced by more than 99 %. Compared with other calibration methods, the better calibration performance of the method in this paper is verified.
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Hua He,Diqiu Liu,Nannan Zhang,Wei Zheng,Qing Han,Bo Ji,Feng Ge,Chaoyin Chen 한국유전학회 2014 Genes & Genomics Vol.36 No.4
Pathogenesis-related (PR) proteins play keyroles in plant responses to pathogens and abiotic stresses. In this study, nine novel PR genes were isolated from Liliumregale Wilson, which is a wild lily species of Chinawith high-level resistance to the soilborne fungal pathogenFusarium oxysporum f. sp. lilii, and homology analysisclassified them into the PR10 family. These novel LrPR10swere clustered together with PR10s from monocotyledonsin a phylogenetic tree, moreover, phylogenetic analysisdivided the nine LrPR10s into two groups. The main-chainconformation and folding patterns of the LrPR10s werehighly conserved with other plant PR10s. The expressionpatterns of the nine LrPR10s in L. regale during normaldevelopment were examined by QRT-PCR, and the transcriptionlevels of the LrPR10s were relatively high inroots. Furthermore, QRT-PCR analysis indicated that theexpression levels of LrPR10-1, LrPR10-2, LrPR10-5,LrPR10-6, and LrPR10-7 in L. regale roots were up-regulatedby two or more stress-related signaling moleculesincluding salicylic acid, jasmonic acid, ethylene, and H2O2,while the other four LrPR10s were repressed by these foursignaling molecules. In addition, five members of theLrPR10 gene family including LrPR10-2, LrPR10-4,LrPR10-5, LrPR10-6, LrPR10-7, and LrPR10-9 werestrongly induced by F. oxysporum in resistant L. regalecompared with the susceptible Lilium Oriental hybrid‘Siberia’. The other four LrPR10s were down-regulated byF. oxysporum infection. In summary, our results indicatethat the members of PR10 gene family are involved inL. regale defense responses against F. oxysporum f. sp. lilii.
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Nannan Zhang,Diqiu Liu,Wei Zheng,Hua He,Bo Ji,Qing Han,Feng Ge,Chaoyin Chen 한국유전학회 2014 Genes & Genomics Vol.36 No.6
The basic leucine zipper (bZIP) proteins areubiquitous in plants and play important roles in plantdefense responses. In this study, based on an expressedsequence tag from a suppression subtractive hybridizationcDNA library of Lilium regale Wilson during Fusariumoxysporum f. sp. lilii infection, a novel bZIP transcriptionfactor gene LrbZIP1 was isolated from L. regale root usingthe rapid amplification of cDNA ends method. The predictedprotein of LrbZIP1 with 142 amino acid residuescontains a basic domain signature and a leucine zippermotif. The quantitative reverse transcription-PCR (qRTPCR)analysis showed that the transcription level of Lrb-ZIP1 was higher in roots of L. regale than in young stemsand leaves. Moreover, the expression of LrbZIP1 was upregulatedin the incompatible interaction between L. regaleand F. oxysporum f. sp. lilii as well as after treatments withstress-related signaling molecules. To verify the function ofLrbZIP1, a constitutive expression vector of LrbZIP1 wasconstructed and transferred into tobacco (Nicotiana tabacumL. cv Xanthi). The results of Southern blotting andqRT-PCR analyses demonstrated that the LrbZIP1 wasintegrated into genome of the tobacco transformants andhighly expressed. Under normal conditions, the T1 transgenictobacco lines showed higher antioxidant enzymeactivities and transcription levels of several resistancerelatedgenes than the wild type. Moreover, the T1 transgenictobacco plants showed strong resistance to F. oxysporumf. sp. lilii infection.
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