Clinical outcomes after sentinel lymph node biopsy in clinically node-negative breast cancer patients

Hee Ji Han,MD,Ju Ree Kim,MD,PhD,Hee Rim Nam,MD,PhD,Ki Chang Keum,MD,PhD,Chang Ok Suh,MD,PhD,Yong Bae Kim,MD,PhD 대한방사선종양학회 2014 Radiation Oncology Journal Vol.32 No.3

Purpose: To evaluate non-sentinel lymph node (LN) status after sentinel lymph node biopsy (SNB) in patients with breast cancer and to identify the predictive factors for disease failure. Materials and Methods: From January 2006 to December 2007, axillary lymph node (ALN) dissection after SNB was performed for patients with primary invasive breast cancer who had no clinical evidence of LN metastasis. A total of 320 patients were treated with breast-conserving surgery and radiotherapy. Results: The median age of patients was 48 years, and the median follow-up time was 72.8 months. Close resection margin (RM) was observed in 13 patients. The median number of dissected SNB was two, and that of total retrieved ALNs was 11. Sentinel node accuracy was 94.7%, and the overall false negative rate (FNR) was 5.3%. Eleven patients experienced treatment failure. Local recurrence, regional LN recurrence, and distant metastasis were identified in 0.9%, 1.9%, and 2.8% of these patients, respectively. Sentinel LN status were not associated with locoregional recurrence (p > 0.05). Close RM was the only significant factor for disease-free survival (DFS) in univariate and multivariate analysis. The 5-year overall survival, DFS, and locoregional DFS were 100%, 96.8%, and 98.1%, respectively. Conclusion: In this study, SNB was performed with high accuracy and low FNR and high locoregional control was achieved.

Clinical outcomes after sentinel lymph node biopsy in clinically node-negative breast cancer patients

Han, Hee Ji,Kim, Ju Ree,Nam, Hee Rim,Keum, Ki Chang,Suh, Chang Ok,Kim, Yong Bae The Korean Society for Radiation Oncology 2014 Radiation Oncology Journal Vol.32 No.3

Purpose: To evaluate non-sentinel lymph node (LN) status after sentinel lymph node biopsy (SNB) in patients with breast cancer and to identify the predictive factors for disease failure. Materials and Methods: From January 2006 to December 2007, axillary lymph node (ALN) dissection after SNB was performed for patients with primary invasive breast cancer who had no clinical evidence of LN metastasis. A total of 320 patients were treated with breast-conserving surgery and radiotherapy. Results: The median age of patients was 48 years, and the median follow-up time was 72.8 months. Close resection margin (RM) was observed in 13 patients. The median number of dissected SNB was two, and that of total retrieved ALNs was 11. Sentinel node accuracy was 94.7%, and the overall false negative rate (FNR) was 5.3%. Eleven patients experienced treatment failure. Local recurrence, regional LN recurrence, and distant metastasis were identified in 0.9%, 1.9%, and 2.8% of these patients, respectively. Sentinel LN status were not associated with locoregional recurrence (p > 0.05). Close RM was the only significant factor for disease-free survival (DFS) in univariate and multivariate analysis. The 5-year overall survival, DFS, and locoregional DFS were 100%, 96.8%, and 98.1%, respectively. Conclusion: In this study, SNB was performed with high accuracy and low FNR and high locoregional control was achieved.

사전연명의료의향서 작성 동기에 관한 연구

장경희(Kyung-Hee Chang),강경희(Kyung-hee Kang),김두리(Doo Ree Kim),임효남(HyoNam Lim),김광환(Kwang-Hwan Kim) 한국산학기술학회 2019 한국산학기술학회논문지 Vol.20 No.10

본 연구의 목적은 호스피스·완화의료와 임종과정에 있는 환자에게 최선의 이익을 보장하고 자기결정을 존중하여 인간으로서의 존엄과 가치를 보호하기 위하여 S시 소재의 C 기독교 종교시설을 방문하여 사전연명의료의향서를 작성한 노인을 대상으로 사전연명의료의향서 작성의 동기를 알아보기 위해 시행되었다. 조사 기간은 2019년 7월 1일부터 10일간 진행하였다. 연구 결과 사전 연명의료 의향서 작성 동기에 관해 4개 주제와 8개의 하위주제가 도출되었다. 주제는 ‘자식들을 위해서’, ‘고통스러울 것 같아서’, ‘내 삶을 잘 정리하고 싶어서’, ‘필요성을 느껴서’ 등의 4가지 주제가 도출되었으며, 하부주제로 ‘자식들 고생시키고 싶지 않아서’, ‘자식들에게 의료비 부담주고 싶지 않아서’, ‘고통에 대한 두려움이 있어서’, ‘고통스러워하는 가족의 임종을 돌본 경험이 있어서’, ‘편안한 마음을 위해서’, ‘자기결정권의 중요성을 느껴서’, ‘그전부터 알고는 있었지만 이번에 결심하게 되어서’, ‘정보를 처음 알게 되었는데 결심하게 되어서’였다. 본 연구결과는 노인들의 좋은 죽음을 위한 웰다잉 교육 프로그램 개선과 사전연명의료의향서 작성을 위한 효과적 제도를 보완하기 위한 기초자료가 될 수 있다는 점에서 의의가 있다. This study examined the patient"s advantage and respect self-decision to protect human dignity and values, who are on the pathway of the hospice palliative care and death process. The study subjects were the elderly who had signed the advance medical directives at C Christian religious facilities in S region. The survey period was started on July 1st, 2019 and lasted for ten days. The study resulted in four topics and eight sub-topics on the motivation for preparing advanced medical directives. The four topics were "for children", "fear of pain", "want to clear my life", "felt the necessity", and the sub-topics were "don"t want to make a burden to children", "don"t want to make a worry of medical expenses", "fear of pain", "have experience of taking caring of painful death process", "relaxed mind", "importance of self-decision", "have known it before but now decided", and "it is the new information and decided". This research is meaningful in that it can form the basis for improving well-dying education programs for the good death of the elderly and supplementing effective systems for preparing advance medical directives.

High Temperature Size Exclusion Chromatography

Hee Sook Cho,Soo Jin Park,Moon Hor Ree,Tai Hyun Chang,Jin Chul Jung,Wang Cheol Zin 한국고분자학회 2006 Macromolecular Research Vol.14 No.3

Regulation of PLK1 through competition between hnRNPK, miR-149-3p and miR-193b-5p

Shin, Chang Hoon,Lee, Hong,Kim, Hye Ree,Choi, Kyung Hee,Joung, Je-Gun,Kim, Hyeon Ho The Author(s) 2017 CELL DEATH AND DIFFERENTIATION Vol.24 No.11

<P>Polo-like kinase 1 (PLK1) is a critical regulator of cell cycle progression and apoptosis. However, its regulation remains poorly understood. In the present study, we investigated the molecular mechanism underlying the post-transcriptional regulation of PLK1. We observed that heterogeneous nuclear ribonucleoprotein K (hnRNPK) and PLK1 were positively associated in several different cancers and high expression levels of them correlated with poor prognosis in patients with cancer. Knockdown of hnRNPK resulted in reduced expression of PLK1, whereas conversely, PLK1 expression was increased in hnRNPK-overexpressing cells. We found that hnRNPK regulated PLK1 expression through KH1- and KH2-dependent interactions with the 3'UTR of PLK1 mRNA. In addition, microRNA-149-3p (miR-149-3p) and miR-193b-5p suppressed PLK1 expression by targeting the 3'UTR of PLK1 mRNA. MicroRNA-elicited enrichment of PLK1 mRNA in Ago2 immunoprecipitation was altered by the presence or absence of hnRNPK. Furthermore, the deletion of the cytosine (C)-rich sequences of the 3'UTR of PLK1 mRNA abolished the decreased PLK1 expression observed via hnRNPK silencing and administration of miRNAs, a finding that suggests that hnRNPK shares this C-rich motif with miR-149-3p and miR-193b-5p. We also found that downregulation of PLK1 by either silencing hnRNPK or overexpression of miR-149-3p and miR-193b-5p decreased clonogenicity and induced apoptosis. Our findings from this study demonstrate that hnRNPK regulates PLK1 expression by competing with the PLK1-targeting miRNAs, miR-149-3p and miR-193b-5p.</P>

High Temperature Size Exclusion Chromatography

Cho Hee-Sook,Park Soo-Jin,Ree Moon-Hor,Chang Tai-Hyun,Jung Jin-Chul,Zin Wang-Cheol The Polymer Society of Korea 2006 Macromolecular Research Vol.14 No.3

High temperature size exclusion chromatography (SEC) has been used widely for the characterization of crystalline polymers, for which high temperature operation above the polymer melting temperature is required to dissolve the polymers. However, this high temperature operation has many advantages in SEC separation in addition to merely increasing polymer solubility. At high temperature the eluent viscosity decreases, which in turn decreases the column backpressure and increases the diffusivity of the analytes. Therefore, many reports on the high temperature operation of high performance liquid chromatography (HPLC) have focused on shortening the analysis time and enhancing the resolution. However, the application of high temperature SEC analysis to exploit the merits of high temperature operation is scarce. In this article, therefore, we report on a new apparatus design for high temperature SEC.

Feasibility Study of a Future Korean Space Telescope

Dae-Hee Lee,Chang Hee Ree,Yong-Seon Song,Woong-Seob Jeong,Hong-Kyu Moon,Min Gyu Kim,Jeonghyun Pyo,Bongkon Moon,Won-Kee Park 한국천문학회 2017 天文學會報 Vol.42 No.1

SRSF3-regulated miR-132/212 controls cell migration and invasion by targeting YAP1

Kim, Hye Ree,Hwang, Su Jin,Shin, Chang Hoon,Choi, Kyung Hee,Ohn, Takbum,Kim, Hyeon Ho Elsevier 2017 Experimental cell research Vol.358 No.2

<P><B>Abstract</B></P> <P>Although SRSF3 (Serine/arginine-rich splicing factor 3) plays a significant role in various biological processes, many of its functions still remain unclear. More particularly, little is known about SRSF3's involvement in the regulation of miRNA. In this report, we found that invasive and migratory abilities were inhibited in SRSF3-silenced U2OS and HeLa cells. We also found that a knockdown of SRSF3 results in a decreased expression level of REST (RE1-silencing transcription factor). The silencing of REST increased the expression of primary miR-132/212 as well as their mature forms. In particular, miR-132-3p and miR-212-3p possess an identical seed sequences and a common target gene. Overexpression of miR-132-3p and miR-212-3p suppressed the expression of YAP1 (Yes-associated protein 1) by directly binding to the 3՚UTR of its mRNA. <I>CCND1</I> (Cyclin D1), which acts downstream of YAP1, was downregulated in both miR-132-3p and miR-212-3p-overexpressed cells, in correlation with diminished YAP1 levels. Taken together, our results reveal that SRSF3 controls the expression of the miR-132/212 cluster through regulating REST expression, and that the REST-elicited alteration of miRNA expression is implicated in enabling the migratory and invasive abilities of cancer cells.</P> <P><B>Highlights</B></P> <P> <UL> <LI> REST is involved in the SRSF3-mediated migration and invasion. </LI> <LI> SRSF3 regulates the expression of miR-132/212 through REST. </LI> <LI> miR-132/212 suppresses YAP1 expression by directly binding to the 3′UTR of its mRNA. </LI> <LI> miR-132/212 inhibits migratory and invasive abilities via downregulation of YAP1/CyclinD1. </LI> <LI> miR-132/212 is responsible for the oncogenic function of SRSF3. </LI> </UL> </P>

시판새우젓 종류별 이화학적ㆍ관능적 특성

오상희(Sang Hee Oh),성태화(Tae Hwa Sung),허옥순(Ok Soon Heo),방옥균(Ok Kyun Bang),장해춘(Hae Choon Chang),신현수(Hyun Soo Shin),김미리(Mee Ree Kim) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.6

시판되는 새우젓을 종류별(오젓, 육젓, 추젓, 자하젓, 새하젓), 제조자별(재래, 기업), 원산지별(국산, 중국산, 베트남산)로 구입하여 그 이화학적, 관능적 특성을 분석, 비교하였다. 기업 새우젓은 염도, VBN 및 AN 등 이화학적 특성들이 제품별로 거의 일정하였던 반면, 재래 새우젓은 제품별로 차이가 많았다. 기업 새우젓은 재래 새우젓에 비해 염도가 높았던 반면, VBN 및 AN 함량은 낮은 경향을 나타내 낮았다. 재래 새우젓은 그 종류별로 다양한 특성을 나타내었는데, 대체적으로 기업 새우젓보다 VBN 및 AN 함량이 높게 나타났다. 새우젓의 VBN 및 AN 함량의 차이는 새우의 어획 및 제조시기의 차이에 따른 차이보다는 그 숙성 정도의 차이로 사료된다. 수입산 추젓의 경우 다른 새우젓에 비해 높은 염도와 낮은 VBN, AN 함량을 보였다. 육젓의 L값과 b값이 높은 반면, 자하젓, 새하젓은 a값이 높았다. 관능검사 결과 재래 새우젓은 기업 새우젓에 비해 외관의 수용도는 낮았으나, 감칠맛, 전반적인 맛, 전반적인 수용도의 점수가 높았다. 새우젓의 종류별로 볼 때, 재래 및 기업 새우젓 모두에서 육젓의 관능평가 점수가 높았으며, 자하젓과 새하젓은 전반적인 맛 및 감칠맛 정도의 점수가 높게 나타났다. 주성분 분석 결과, 주성분 PC1에 대해 재래새우젓에서는 각각 새하젓, 오젓, 자젓이 기업새우젓에서는 육젓이 그리고 주성분 PC2에 대해서는 재래새우젓의 경우 자젓, 육젓이 기업새우젓에서는 추젓이 양의 방향으로 부하되고 있었다. 또한 전반적인 수용도와 가까이 위치한 이화학적 특성으로는 재래새우젓에서는 a값, TBA, AN값이었으며, 기업새우젓에서는 L값과 b값이었다. We evaluated physicochemical and sensory characteristics of 25 commercial salt-fermented shrimps by kind (Oh Jeot, Yook Jeot and Chu Jeot) by manufacturer (traditional marketer (TS) & company (CS)). Salinity and pH ranged 17.9~28.7% and 7.82~8.74, respectively, of which Chu Jeot was somewhat higher in salinity and pH, compared with those of the others. Amino nitrogen (AN), volatile basic nitrogen (VBN) and thiobarbituric acid reactive subjects (TBARS) showed great variation ranged with 21.41~661.13 mg%, 263.2~1180.2 mg% and 0.507~1.322 ㎍/g, respectively. Hunter color of L value was 53.99~67.45, a value, 4.98~12.06 and b value, 4.45~10.4. Physicochemical quality showed greater variations in Chu Jeot of TS than that of CS. Products of CS have higher salinity while lower VBN and AN than those of TS. Sensory results showed that mean scores of appearance, over-all taste, over-all flavor and over-all acceptability between TS and CS were not significantly different. The mean score of over-all acceptance was the highest in Yook Jeot. Physicochemical and sensory characteristics of salt-fermented shrimps in a PCA plot comprised of first principal component (68.36%) and second principal component (31.36%).

Development of the Earth Observation Camera of MIRIS

Lee, Dae-Hee,Han, Won-Yong,Park, Young-Sik,Park, Sung-Jun,Moon, Bong-Kon,Ree, Chang-Hee,Pyo, Jeong-Hyun,Jeong, Woong-Seob,Nam, Uk-Won,Lee, Duk-Hang,Park, Kwi-Jong,Bae, Soo-Ho,Rhee, Seung-Wu,Park, Jong 한국우주과학회 2011 Journal of Astronomy and Space Sciences Vol.28 No.3