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( Chaiwat Monmai ),( A-yeong Jang ),( Ji-eun Kim ),( Sang-min Lee ),( Sangguan You ),( Seokbeom Kang ),( Tae Ho Lee ),( Woo Jung Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2020 Journal of microbiology and biotechnology Vol.30 No.12
Tunicates are known to contain biologically active materials and one species in particular, the sea peach (Halocynthia aurantium), has not been thoroughly studied. In this study we aimed to analyze the fatty acids profile of the H. aurantium body wall and its immunomodulatory effects on RAW264.7 macrophage-like cells. The fatty acids were classified into three categories: saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs). Omega-3 fatty acid content, including EPA and DHA, was higher than omega-6 fatty acids. H. aurantium body wall fatty acids exhibited enhanced immune response and anti-inflammatory effects on RAW264.7 macrophage-like cells. Under normal conditions, fatty acids significantly increase nitric oxide (NO) and PGE2 production in a dose-dependent manner, thereby improving the immune response. On the other hand, in LPS-treated RAW264.7 cells, fatty acids significantly decreased nitric oxide (NO) and PGE2 production in a dose-dependent manner, thereby enhancing anti-inflammatory effects. Fatty acids transcriptionally control the expression of the immune-associated genes, iNOS, IL-1β, IL-6, COX-2, and TNF-α, via the MAPK and NF-κB signaling cascades in RAW264.7 cells. However, in LPSstimulated RAW264.7 cells, H. aurantium body wall fatty acids significantly inhibited expression of inflammatory cytokine; similarly, production of COX-2 and PGE2 was inhibited. The results of our present study provide insight into the immune-improving and anti-inflammatory effects of H. aurantium body wall fatty acids on macrophages. In addition, our study demonstrates that H. aurantium body wall is a potential source of immune regulatory components.
Monmai, Chaiwat,Park, Sung Hee,You, SangGuan,Park, Woo Jung 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
Polysaccharide (SJP) was extracted from Sea cucumber, Stichopus japonicas, and its immune-enhancing activities were evaluated in vivo immune-suppressed mice systems. Cyclophosphamide(CY)-treated mice were orally administrated with SJP according to different concentrations. The results showed that administration of SJP significantly increased spleen index without variation of the body weight, compared to only CY treatment group. The proliferation of splenic lymphocyte and NK activity was also stimulated by SJP. In addition, the oral administration of SJP up-regulated COX-2 and TLR-4 as well as cytokines such as $IL-1{\beta}$, IL-4, IL-6, IL-10, $TNF-{\alpha}$ and $IFN-{\gamma}$, which are secreted from splenic lymphocytes in cyclophosphamide-treated mice. Moreover, our results showed that SJP stimulated macrophages via $NF-{\kappa}B$ and MAPK signaling pathways. These findings provided the potential use of SJP as an alternative means under immune-suppressed conditions, and furthermore can be utilized as a functional material for food and pharmaceutical industries.
Chaiwat Monmai,박성희,유상관,박우정 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.2
Polysaccharide (SJP) was extracted from Sea cucumber, Stichopus japonicas, and its immune-enhancing activities were evaluated in vivo immune-suppressed mice systems. Cyclophosphamide(CY)-treated mice were orally administrated with SJP according to different concentrations. The results showed that administration of SJP significantly increased spleen index without variation of the body weight, compared to only CY treatment group. The proliferation of splenic lymphocyte and NK activity was also stimulated by SJP. In addition, the oral administration of SJP up-regulated COX-2 and TLR-4 as well as cytokines such as IL-1b, IL-4, IL-6, IL-10, TNF-a and IFN-c, which are secreted from splenic lymphocytes in cyclophosphamide- treated mice. Moreover, our results showed that SJP stimulated macrophages via NF-jB and MAPK signaling pathways. These findings provided the potential use of SJP as an alternative means under immune-suppressed conditions, and furthermore can be utilized as a functional material for food and pharmaceutical industries.
( Chaiwat Monmai ),( Seok Hyeon Go ),( Il-shik Shin ),( Sangguan You ),( Hyungjae Lee ),( Seokbeom Kang ),( Woo Jung Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.3
Asterias amurensis is a marine organism that causes damage to the fishing industry worldwide; however, it has been considered a promising source of functional components. The present study aimed to investigate the immune-enhancing effects of fatty acids from three organs of A. amurensis on murine macrophages (RAW 264.7 cells). A. amurensis fatty acids boosted production of immune-associated factors such as nitric oxide (NO) and prostaglandin E2 in RAW 264.7 cells. A. amurensis fatty acids also enhanced the expression of critical immuneassociated genes, including iNOS, TNF-α, IL-1β, and IL-6, as well as COX-2. Western blotting showed that A. amurensis fatty acids stimulated the NF-κB and MAPK pathways by phosphorylation of NF-κB p-65, p38, ERK1/2, and JNK. A. amurensis fatty acids from different tissues resulted in different levels of NF-κB and MAPK phosphorylation in RAW 264.7 cells. The results increase our understanding of how A. amurensis fatty acids boost immunity in a physiological system, as a potential functional material.
Chaiwat Monmai,남주현,임준혁,Weerawan Rod-in,이태호,박우정 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.11
Rice cake is a traditional food in Korea, and ismade by rice alone, or with other grain powder. Toimprove the health benefits of fermented rice cake, the ricepowder was supplemented with strawberry powder. Antiinflammatoryactivities of the mixture of strawberry andrice powder were evaluated. Treatment with the mixturesignificantly decreased the production of nitric oxide (NO). The mixture of strawberry and rice powder in the ratio 10:90 effectively and dose-dependently reduced the immuneassociatedgenes iNOS, IL-1b, IL-6, COX-2, and TNF-a. Furthermore, carrageenan-injected mice were used to studythe anti-inflammatory effect of the mixture. Pre-oraladministration of the mixture of strawberry and rice powderat doses of 50 and 100 mg/kg BW significantly reducedpaw edema induced by carrageenan. These results suggestthat for fermented rice cake production and processing, thestrawberry and rice powder mixture may be a potentialsource of anti-inflammatory activity.
( Chaiwat Monmai ),( Seok Hyeon Go ),( Il-sik Shin ),( Sangguan You ),( Dae-ok Kim ),( Seokbeom Kang ),( Woo Jung Park ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.10
Asterias amurensis (starfish) is a marine organism that is harmful to the fishing industry, but is also a potential source of functional materials. The present study was conducted to analyze the profiles of fatty acids extracted from A. amurensis tissues and their anti-inflammatory effects on RAW264.7 macrophage cells. In different tissues, the component ratios of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids differed; particularly, polyunsaturated fatty acids such as dihomo-gamma-linolenic acid (20:3n-6) and eicosapentaenoic acid (20:5n-3) were considerably different. In lipopolysaccharide-stimulated RAW264.7 cells, fatty acids from A. amurensis skin, gonads, and digestive glands exhibited anti-inflammatory activities by reducing nitric oxide production and inducing nitric oxide synthase gene expression. Asterias amurensis fatty acids effectively suppressed the expression of inflammatory cytokines such as tumor necrosis factor-α, interleukin-1β, and interleukin-6 in lipopolysaccharide-stimulated cells. Cyclooxygenase-2 and prostaglandin E2, which are critical inflammation biomarkers, were also significantly suppressed. Furthermore, A. amurensis fatty acids reduced the phosphorylation of nuclear factor-κB p-65, p38, extracellular signal-related kinase 1/2, and c-Jun N-terminal kinase, indicating that these fatty acids ameliorated inflammation through the nuclear factor-κB and mitogen-activated protein kinase pathways. These results provide insight into the anti-inflammatory mechanism of A. amurensis fatty acids on immune cells and suggest that the species is a potential source of anti-inflammatory molecules.