Validity of Three-dimensional Superimposition of Whole Face according to Different Registration Areas

Min-Hee Oh,Chaeyong Jung,Sang-Woon Jeon,Jin-Hyoung Cho 대한치의학회 2019 Journal of korean dental science Vol.12 No.2

Bilateral asymmetric supernumerary heads of biceps brachii

Song Eun Lee,Chaeyong Jung,Kyu Youn Ahn,Kwang Il Nam 대한해부학회 2011 Anatomy & Cell Biology Vol.44 No.3

Anatomical variations of the biceps brachii have been described by various authors, but the occurrence of bilateral asymmetric supernumerary heads is rare and has not been reported. We found three accessory heads of the biceps brachii muscle on right arm and an anomalous third head of biceps brachii on left arm. The third, fourth, and fifth heads of right arm originated from the body of humerus at the insertion site of coracobrachialis and inserted into the distal part of biceps brachii short head in order. The third head of left arm originated from humerus at the insertion site of coracobrachialis and combined with the distal part of biceps brachii and continued to the proximal part of common biceps tendon. Understanding the existence of bilateral asymmetric supernumerary heads of biceps brachii may influence preoperative diagnosis and surgery on the upper limbs.

태반-특이유전자의 흰쥐 태반내 발현분포

최은화(Eun Hwa Choi),정채용(Chaeyong Jung),남광일(Kwang Il Nam),이승원(Seung Won Lee),배춘상(Choon Sang Bae),김백윤(Baik Yoon Kim),박성식(Sung Sik Park),안규윤(Kyu Youn Ahn) 대한체질인류학회 2011 해부·생물인류학 (Anat Biol Anthropol) Vol.24 No.1

Homeobox 유전자는 분화, 발생 및 발암 등을 유도하는 유전자들의 발현을 조절하는 많은 전사인자들을 암호화하고 이 유전자의 특징은 서로 다른 종 간에도 일정한 염기서열이 잘 보존되어 있다는 것이다. 본 연구는 homeobox 유전자계에 속하는 흰쥐의 새로운 태반-특이 유전자(rPsx)로 부터 digoxigenin이 표지된 cRNA probe를 만들어 in situ hybridization 조직화학 기법을 이용하여 임신 7.5일부터 16.5일까지의 흰쥐 태반에서 이의 발현 및 분포를 알아보고자 하였다. In situ hybridization 조직화학 소견에서 임신 10.5일에 처음으로 태반-특이유전자의 mRNA가 융모막외배엽에서 처음으로 발현되기 시작하였다. 임신 11.5일에서 hybridization signal은 미로영양막층과 거대영양막세포층에서만 관찰되었다. 임신 12.5, 13.5 및 14.5일에서는 hybridization signal은 거대영양막세포층, 해면영양막층 및 미로 영양막층에서 관찰되었다. 임신 15.5일에서는 hybridization signal이 미로영양막층과 해면체영양막층에서 관찰되었으나 거대영양막세포층에서는 관찰되지 않았다. 해면체영양막층에서의 hybridization signal은 임신 14.5일에 비해 상당히 감소하였다. 임신 16.5일에서는 hybridization signal이 미로영양막층에서만 관찰되었고 해면체영양막층이나 거대영양막세포층에서는 관찰되지 않았다. 임신 경과에 따른 hybridization signal은 임신 10.5일부터 점차 증가하여 14.5일에 최고조에 달한 후 그 이후에는 감소하는 경향이었다. 이상의 결과로 흰쥐에서 새로운 태반-특이유전자는 임신 10.5일부터 발현되기 시작하여 태반의 융모막외배엽, 미로영양막층, 해면영양막층 및 거대 영양막세포층에서만 발현되며 영양막세포의 분화 및 발육에 중요한 역할을 할 것으로 생각되었다. Homeobox genes seem to play critical roles in regulating morphogenesis, patterning, organogenesis, and differentiation. They have the conserved sequence that codes the DNA-binding domain called homeodomain. The expression and cellular localization of rPsx mRNA in rat placenta during placental development were examined by in situ hybridization histochemistry at different embryonic stages (Embryonic days 7.5~16.5). rPsx mRNA was first detected in chorionic ectoderm of placenta at E 10.5. This transcript was localized in labyrinth trophoblast and trophoblast giant cells at E 11.5. Hybridization signals were observed in labyrinth trophoblast, spongiotrophoblast, and trophoblast giant cells at E 12.5, E 13.5, and E 14.5. At E 15.5, hybridization signal was detected in labyrinth trophoblast and spongiotrophoblast but not in trophoblast giant cells. Hybridization signal was only detected in labyrinth trophoblast at E 16.5. rPsx mRNA was not detected in decidua and any tissues of the embryo from E 7.5 to E 9.5 of gestations. From these results, a new rPsx homeobox gene is first expressed at E 10.5 and detected in chorionic ectoderm, labyrinth trophblast, spongiotrophoblast and trophoblast giant cells of the placenta. This gene may play a critical role in differentiation and development of trophoblast cells.

치조열을 동반한 편측성 구순열에서 왜소치 배열을 통한 치조골 형태 개선

김도길(Do-Gil Kim),정채용(Chaeyong Jung),이경민(Kyung-Min Lee),조진형(Jin-Hyoung Cho),오민희(Min-Hee Oh) 대한구순구개열학회 2023 대한구순구개열학회지 Vol.26 No.2

This case report describes a successful orthodontic treatment with orthognathic surgery of a 17-year-old girl with skeletal Class III and unilateral cleft lip and alveolus on left side. The treatment included extraction of the maxillary premolars and asymmetric mandibular setback surgery. To induce regeneration of buccal alveolar bone on upper left lateral incisor, the palatal positioned left lateral incisor was aligned. After orthodontic treatment with orthognathic surgery, the patient had an improved facial profile, improved alveolar bone appearance on the cleft alveolus area, and favorable occlusion.

Apoptotic Effects of 6-Gingerol in LNCaP Human Prostate Cancer Cells

Hyun-Woo Kim,Deuk-Hee Oh,Chaeyong Jung,Dong-Deuk Kwon,Young-Chai Lim 순천향대학교 순천향의학연구소 2011 Journal of Soonchunhyang Medical Science Vol.17 No.2

Objective: 6-Gingerol, one component of ginger (Zingiber officinale) compound, has been known to possess anti-inflammatory, analgesic, anti-emetic, and anti-cancer effects. In this study, the apoptotic ability of 6-gingerol was investigated in human prostate cancer cells. Methods: 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide (MTT) assay, flow cytometry, and western blot analysis were done in LNCaP human prostate cancer cell lines treated with the various doses of 6-gingerol for the different durations of drug exposure. Results: 6-Gingerol in doses ranging from 100 to 300 μM induced dose- and time-dependent inhibition of cell viability in prostate cancer cells by using MTT assay. Maximal inhibition of cell viability was observed at 300 μM of 6-gingerol for 48 hours treatment in LNCaP cells. 6-Gingerol at the dose of 100 μM did not produce any significant change in apoptotic cells in flow cytometry analysis. However, significant increase in sub-G0/G1 phase was observed in cells treated with 200 and 300 μM of 6-gingerol. Any significant cell cycle arrest was not induced by 6-gingerol. In western blotting analysis, expression of caspase-3 was not evident in cells treated with 6-gingerol for 24 hours. However, 48 hours treatment with 6-gingerol altered the expression of caspase-3 in LNCaP cells. Expression of cleaved poly showed the dose-dependent fashion in both 24 hours and 48 hours treatment of 6-gingerol. Conclusion: These observations suggest that 6-gingerol may induce apoptosis in LNCaP human prostate cancer cells.

저칼륨혈증 흰쥐 신장에서 Carbonic anhydrase Ⅱ 발현 증가

이용찬(Yong Chan Lee),정채용(Chaeyong Jung),남광일(Kwang Il Nam),이승원(Seung Won Lee),배춘상(Choon Sang Bae),김백윤(Baik Yoon Kim),박성식(Sung Sik Park),안규윤(Kyu Youn Ahn) 대한체질인류학회 2011 해부·생물인류학 (Anat Biol Anthropol) Vol.24 No.1

산-염기 평형장애나 전해질 불균형은 신요세관에서 HCO₃? 재흡수가 증가되거나 감소되는 것과 연관이 있다고 알려져 있다. 칼륨제한시 대사성 알칼리증을 유발시키고 이와 관련된 Na/H exchanger와 Na/HCO₃ cotransporter에 대한 연구는 잘 알려져 있지만 저칼륨혈증시 carbonic anhydrase에 관한 연구는 없다. 본 연구는 흰쥐신장에서 칼륨제한 식이 시기에 따라 carbonic anhydrase Ⅱ의 신장내 발현 및 분포의 변화를 Western 분석과 면역조직화학적 방법으로 관찰하였다. Western 분석 소견에서 carbonic anhydrase Ⅱ 단백은 30 kDa 정도이고 정상식이 군에서 상당량 발현되었으며 칼륨제한 식이 군에서는 정상식이 군에 비해 약간 증가하는 경향이었다. 면역조직화학 소견에서 carbonic anhydrase Ⅱ 단백은 겉질집합관, 바깥속질집합관 및 속속질집합관의 상 1/3에서만 발현되었다. 면역반응성은 속속질 집합관으로 갈수록 감소하였다. 집합관에서의 면역반응성은 개재세포가 강했으며 주세포는 미약하였다. 칼륨제한 식이 군에서 면역반응 부위는 정상식이 군과 차이가 없었다. 면역반응성은 겉질집합관 특히 일부 개재세포와 주세포에서 감소하였고 바깥속질 바깥줄무늬층 집합관에서는 변화가 없었다. 이와는 대조적으로 바깥속질속줄무늬층 집합관과 속속질집합관 상 1/3에서는 현저히 증가하였다. 이상의 소견은 저칼륨혈증시 신장 집합관에서의 carbonic anhydrase Ⅱ 단백은 부위에 따라 발현양상이 다름을 암시하였다. A number of acid-base or electrolyte disorders are associated with decreased or increased HCO₃? reabsorption in the renal tubules. The present study was to examine the alterations of expression and distribution of Carbonic anhydrase Ⅱ in the kidneys of normal and potassium-depleted rats using Western blot analysis and immuno-histochemistry. Western blot analysis demonstrated that CA Ⅱ protein, ~30 kDa at molecular mass, was abundantly expressed in normal group. All potassium-depleted groups showed slightly increased CA Ⅱ protein compared to normal group. In control group, immunoreactivity of CA Ⅱ protein was detected in the entire collecting duct. Signal intensity was prominent in the intercalated cells and weak in the principal cells of the cortical collecting ducts. In potassiumdepleted groups, the pattern of cellular labeling of CA Ⅱ protein was identical to that of normal group, but the signal intensity was decreased in cortical collecting duct, markedly increased in the inner stripe of outer medullary and inner medullary collecting ducts, and unchanged in the outer stripe of outer medullary collecting duct. These results suggest that chronic hypokalemia impact the expression pattern of CA Ⅱ protein depending the portion of the collecting duct.

HOXB13 regulates the prostate-derived Ets factor: Implications for prostate cancer cell invasion

KIM, IN-JE,KANG, TAEK WON,JEONG, TAEOH,KIM, YOUNG-RANG,JUNG, CHAEYONG Spandidos Publications 2014 International journal of oncology Vol.45 No.2

<P>HOXB13 has been shown to enhance the invasive potential of breast and endometrial tumors. HOXB13 is also abundant in castration-resistant prostate tumors. To determine the invasive potential of HOXB13 in prostate tumors, highly metastatic PC3 prostate cancer cells were manipulated to express HOXB13 and/or the prostate-derived Ets factor (PDEF). The PDEF is believed to reduce the invasive potential of various tumors, including prostate tumors. To further demonstrate the functional correlation between HOXB13 and PDEF, transwell invasion and gelatin zymography assays were performed. In addition, the western blot analysis was used to demonstrate the expression of PDEF target proteins involved in cancer cell migration and invasion, MMP-9 and survivin. According to the results, HOXB13 promoted PC3 cell migration and invasion. The DNA microarray analysis demonstrated that HOXB13 significantly suppressed the expression of the PDEF. Accordingly, the expression of MMP-9 and survivin was regulated by HOXB13. In addition, HOXB13 promoted the invasive potential of PC3 cells while inhibiting the PDEF. The coexpression of HOXB13 and the PDEF led to moderate retardation of the number of invasive cells, indicating that HOXB13 functionally counteracted cell invasion by reducing PDEF expression. The western blot analysis demonstrated that HOXB13 counteracted the PDEF-mediated inhibition of the expression of PDEF target proteins such as MMP-9 and survivin. The results suggest that the HOXB13-mediated promotion of tumor cell invasion is accomplished mainly through the downregulation of PDEF expression.</P>

Gene expression profiling of mouse aborted uterus induced by lipopolysac charide

Jeong Mi Moon,Song Eun Lee,Yong Il Min,Chaeyong Jung,Kyu Youn Ahn,Kwang Il Nam 대한해부학회 2011 Anatomy & Cell Biology Vol.44 No.2

To identify genes that participate in the abortion process, normal pregnant uteri were compared to lipopolysaccharide (LPS)-induced abortion uteri. At day 6 of pregnancy, mice were treated with LPS at various time points to induce an abortion. Total RNAs were applied to a cDNA microarray to analyze genes with altered expression. At the early stage (2 hours) of LPS-induced abortion, upregulated genes were mainly composed of immune responsive genes, including Ccl4, Ccl2, Cxcl13, Gbp3, Gbp2, Mx2, H2-Eb1, Irf1 and Ifi203. Genes related to toll-like receptor signaling were also overexpressed. At late stages of abortion (12-24 hours), many genes were suppressed rather than activated, and these were mainly related to the extracellular matrix, cytoskeleton, and anti-apoptosis. Altered expression of several selected genes was confirmed by real time reverse transcription-polymerase chain reaction. Th e results demonstrated that many known genes were altered in the LPS-treated pregnant uterus, implying that the molecular mechanisms of the genes involved in LPS-induced abortion are complicated. Further analysis of this expression profi le will help our understanding of the pathophysiological basis for abortion.

흰쥐 침샘에서 Carbonic Anhydrase 동위효소 IV와 IX의 분포에 관한 면역조직화학적 연구

조태영(Tae Young Cho),이송은(Song Eun Lee),남광일(Kwang Il Nam),정채용(Chaeyong Jung),안규윤(Kyu Youn Ahn),배춘상(Choon Sang Bae),김백윤(Baik Yoon Kim),박성식(Sung Sik Park) 대한해부학회 2009 Anatomy & Cell Biology Vol.42 No.4

침샘에서 carbonic anhydrase(CA) 동위효소의 발현 여부를 관찰하고자 흰쥐 귀밑샘과 턱밑샘을 대상으로 CAs I, II, IV 및 IX 동위효소에 대한 항체를 사용하여 면역조직화학 염색을 시행하였고, 이들 조직에서 각각의 CA 동위효소 단백발현을 Western blot 분석을 시행하여 확인하였다. Western blot 분석 결과 귀밑샘은 CAs I, II와 IX는 강하게 발현되었고 CA IV는 미약하게 발현되었다. 턱밑샘에서는 CAs I과 II는 강하게 발현되었으나 Cs IX는 미약하게 발현하게 발현되었고 CA IV는 발현되지 않았다. H-E 염색에서 귀밑샘은 장액샘꽈리와 도관으로 구성되어 있었으며 턱밑샘은 샘의 대부분을 차지하는 고유턱밑샘형소엽과 점액샘꽈리와 장액반달로 구성된 혀밑샘형 소엽으로 되어 있었다. 면역조직화학 염색에서 귀밑샘은 CA IV, IX 및 I의 반응은 사이관, 줄무늬관 및 소엽사이관 같은 도관세포에서 양성을 보이고 샘꽈리세포에서는 음성이었다. CA II 반응은 도관세포와 샘꽈리세포에서 양성이었다. 턱밑샘의 고유턱밑샘형 소엽에서 CA IV, IX 및 I의 반응은 도관세포에서는 양성 반응을 보였으나 샘꽈리세포에서는 음성이었다. CA II 반응은 도과세포와 장액샘꽈리세포에서 모두 양성이었다. 턱밑샘의 혀밑샘형 소엽에서 CAs IV, IX 및 I 반응은 도관세포에서는 양성을 보였으나 샘꽈리세포에서는 음성이었다. CA II 반응은 도과세포와 장액반달세포에서 양성이었으나 점액샘꽈리세포에서는 음성이었다. 이상의 관찰로 흰쥐 귀밑샘과 턱밑샘에 분포하는 CA 동위 효소의 분포를 확인하였으며, 침 도관세포에서의 전해질 대사는 CAs I과 II뿐 아니라 CAs IV와 IX도 관여할 것으로 추측되었다. This study presents distribution of carbonic anhydrase(CA) isozymes IV and IX, membrane associated forms, and CA I and II, cytoplasmic forms, in rat parotid and submandibular glands using Western blot analysis and immunohistochemical staining. Western blot analysis demonstrated that CAs I, II and IX were found to be abundantly expressed, but CA IV was weakly expressed in parotid gland. Submandibular gland expressed abundant CAs I and II, weak CA IX, and undetectable level of CA IV. In hematoxylin-eosin staining, parotid gland was entirely composed of serous acini and their ducts while submandibular gland was mixed population of serous and mucous lobules. Most of lobules (submandibular gland proper type) contained mostly serous acini and their ducts with granular convoluted duct. Some lobules (sublingual gland type) contained mostly mucous acini with serous demilune and their ducts without granular convoluted duct. In parotid gland, CAs IV and IX were immunolocalized in duct cells and not in serous acinar cells. Immunoreactivity for CAs I and II was also detectavle in duct cells. Serous acinar cells were positive for CA II, and negative for CA I. In submandibular gland, CAs IV and IX were immunolocalized in duct cells but not in acinar cells of both types of lobules. Immunoreactivity for CAs I and II was also detectable in duct cells of both types of lobules. Cells of serous acini and serous demilune were positive for CA II, and negative for CA I. Mucous cells were negative for both CAs I and II. These results demonstrate the distribution of CA isoenzymes in parotid and submandibular glands of the rat, and suggest CAs IV and IX as well as CAs I and II are related to electrolytes metabolism of saliva in duct cells.

Zinc Inhibits Expression of Androgen Receptor to Suppress Growth of Prostate Cancer Cells

To, Phuong Kim,Do, Manh-Hung,Cho, Young-Suk,Kwon, Se-Young,Kim, Min Soo,Jung, Chaeyong MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.10

<P>The prostate gland contains a high level of intracellular zinc, which is dramatically diminished during prostate cancer (PCa) development. Owing to the unclear role of zinc in this process, therapeutic applications using zinc are limited. This study aimed to clarify the role of zinc and its underlying mechanism in the growth of PCa. ZnCl<SUB>2</SUB> suppressed the proliferation of androgen receptor (AR)-retaining PCa cells, whereas it did not affect AR-deficient PCa cells. In LNCaP and TRAMP-C2 cells, zinc downregulated the expression of AR in a dose- and time-dependent fashion. Zinc-mediated AR suppression accordingly inhibited the androgen-mediated transactivation and expression of the androgen target, prostate specific antigen (PSA). This phenomenon resulted from facilitated protein degradation, not transcriptional control. In studies using mice bearing TRAMP-C2 subcutaneous tumors, the intraperitoneal injection of zinc significantly reduced tumor size. Analyses of both xenograft tumors and normal prostates showed reduced expression of AR and increased cell death. Considering the significant loss of intracellular zinc and the dominant growth-modulating role of AR during PCa development, loss of zinc may be a critical step in the transformation of normal cells to cancer cells. This study provides the underlying mechanism by which zinc functions as a PCa suppressor, and forms the foundation for developing zinc-mediated therapeutics for PCa.</P>