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Oh, Sung Ki,Storm, Carlyle B. 경희대학교 부설 식량자원개발연구소 1978 硏究論文集 Vol.1 No.-
펲타이드 加水分解用試藥으로서 [Co(bpdah)CO₃]ClO₄를 合成하였으며 蛋日質乃至는 펲타이드의 加水分解物分들을 同定하는데 쓰일 [Co(bpdah)(L-Leu)](ClO₄)₂, [Co(bpdah)(A-Ala)](CIO₄)₂, [Co(bpdah)(DL-Val)(ClO₄)₂, [Co(bpdah)(Gly)](ClO₄)₂ 및 [Co(bpdah)(DL-Phe)]I₂ㆍxH₂O의 아미노酸錯化合物을 合成하였다. 溫度와 pH가 Co(bpdah)CO₃^(+), Co(bpdah)(H₂O)₂^(3+), CO(bpdah)(OH)(H₂O)^(2+) 이온들에 미치는 影響을 素外線分光器械로 調査한 結果 이들 이온 特히 CO(bpdah)(OH)(H₂O)^(2+) 이온은 酸性과 微알칼리水溶液中에서 安定하였다. 또 CO(bpdah)(OH)(H₂O)^(2+)에 L-Ala-L-Phe을 反應시켜 본 結果 CO-(bpdah)(OH)( H₂O)^(2+) 이온의 素外線分光스펙트럼의 吸光度는 時間經過에 따라 漸漸 減少하였다.
Evaluation of Exogenous Promoters for Use in Brachiaria brizantha Transformation
Silveira Erica Duarte,Rodrigues Julio Carlyle Macedo,Cabral Glaucia Barbosa,Leite Juliana de Almeida,Costa Sidnei Souza,Carneiro Vera Tavares de Campos The Korean Society of Plant Biotechnology 2003 Plant molecular biology and biotechnology research Vol.5 No.2
Brachiaria (Poaceae) is the most important forage genus for cattle production in Brazil. The genetic breeding of this genus is limited by the incompatibility among species, differences in ploidy level and the natural cloning of plants by apomixis (Valle and Miles 1992). However, plant regeneration via tissue culture methods and genetic engineering provide an opportunity to introduce new characteristics in plants of this genus. We have developed methods for the 'genetic modification of Brachiaria brizantha cv. Marandu via biolistic transformation. A higher number of shoots was obtained with 4 mg/L 2.4-diclorophenoxyacetic acid and 0.2 mg/L benzylaminopurine in calli induction medium and 0.1 mg/L naphtaleneacetic acid and 4.0 mg/L kinetin in shoot regeneration medium. A selection curve for mannose was determined to use phospho mannose isomerase (PMI) gene of Escherichia coli as a selection marker. Calli formation was inhibited from 5 g/L mannose, even in the presence of sucrose while calli that were formed in the presence of mannose failed to develop embryos showing that PMI gene can be used for selection of transformants of this grass. Different promoters were tested to evaluate the efficiency based on the detection of the GUS gene expression (Jefferson et al. 1987). The monocot promoters, act1-D and ubi-1, resulted in higher expression levels than dicot promoters, ubi-3 and act-2, or the CaMV35S and CVMV promoters.
Lim, Sang-Sun,Baah-Acheamfour, Mark,Choi, Woo-Jung,Arshad, Muhammad A.,Fatemi, Farrah,Banerjee, Samiran,Carlyle, Cameron N.,Bork, Edward W.,Park, Hyun-Jin,Chang, Scott X. Elsevier 2018 Forest ecology and management Vol.417 No.-
<P><B>Abstract</B></P> <P>Our understanding of the effect of agroforestry systems on soil organic carbon (SOC) is largely limited to the upper layer of the mineral soil, while LFH (litter, partially decomposed litter and humus) and deeper soil layers are poorly studied. In this study, the effects of three different agroforestry systems (hedgerow, shelterbelt, and silvopasture) and their component land-cover types (treed area and adjacent herbland) on SOC stock in LFH and mineral soil layers (0–75 cm) were investigated across 36 sites in central Alberta, Canada. The SOC stock of mineral soil (0–75 cm) was not affected by agroforestry systems but by land-cover type. The treed area had greater (<I>p</I> < 0.001) SOC in the 0–75 cm mineral soil (25.5 kg C m<SUP>−2</SUP>) than the herbland (19.4 kg C m<SUP>−2</SUP>), driven by the greater (<I>p</I> < 0.001) SOC level in the top 0–30 cm rather than that in the deeper layers (30–75 cm). Within the treed area, the silvopasture system that was dominated by broad-leaf deciduous trees had 56–70% more SOC in the 0–10 cm soil than in the hedgerow and shelterbelt systems. The SOC stock in the 0–10 cm layer was positively (<I>p</I> = 0.025) related to the C stock of the overlying LFH layer in the silvopasture system. These results together with the 22–24% higher dissolved organic carbon (DOC) concentration in the silvopasture than in the other systems suggest that the greater SOC stock in the 0–10 cm mineral soil could be attributed to the higher rates of translocation of DOC from the LFH in the silvopasture than that in shelterbelt or hedgerow. We conclude that SOC stock in the top mineral soil (e.g., 0–30 cm) is more responsive to changes in land-cover type and the LFH layer plays an important role in increasing SOC stock in the surface mineral soil of the agroforestry systems in central Alberta.</P> <P><B>Highlights</B></P> <P> <UL> <LI> In agroforestry systems, treed area had greater SOC than herbland. </LI> <LI> Silvopasture had greater SOC than hedgerow and shelterbelt. </LI> <LI> LFH layer played a critical role in increasing SOC in mineral layer. </LI> <LI> SOC storage in 0–30 cm mineral layer was most responsive to land-cover type. </LI> </UL> </P>