http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
오징어 연골을 이용한 Chitosan 및 N - acetylchitosan film 의 제조 및 특성
최현미(Hyeon Mee Choi),이근태(Keun Tai Lee) 한국수산과학회 2000 한국수산과학회지 Vol.33 No.4
In order to utilize the processing wastes of squid, chitosan was prepared by intermittent deacetylation treatment of β-chitin richly contained in the pen of squid, and then their characteristics of chitosan and N-acetylchitosan film were studied. The acetylation time of N-acetylchitosan film-1 (N-ACF-1) manufactured from chitosan solution by treating with acetic anhydride was about 12 hrs. In SEM photomicrographs, the surface of chitosan film was regularly arranged netlike, and that of N-acetylchitosan film-2 (N-ACF-2) was rough like snowflake and larger than chitosan film. The chitosan film (thickness 0.02㎜, time 60 min) had the highest tensile strength (1,240㎏/㎠) and elongation (58.25%), N-ACF-1 (thickness 0.02㎜, time 60 min) had the highest water permeability (539 g/㎡·24 hrs), oxygen permeability (20,000 ㎤/㎡·24 hrs·atm) and water uptake (350%) among the tested films.
오징어 연골을 이용한 Chitosan 및 Acetylchitosan 의 제조와 특성
최현미(Hyeon Mee Choi),황선영(Sun Young Hwang),박성민(Seong Min Park),이근태(Keun Tai Lee) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.5
1. 오징어 연골과 chitin, chitosan의 질소함량은 각각 12.1%, 8.1%, 5.8%이며 회분함량은 각각 0.8%, 9.4%, 0.2%로 나타났으며 오징어 연골로부터 최종적으로 만들어지는 chitosan의 수율은 25土 3% 이었다. 2. 재탈아세틸화 하여 얻어진 chitosan의 탈아세틸화도는 92%로 이는 NMR을 분석한 결과 chitin의 NMR 분석에서 보여지는 CH₃와 C=O기의 peak가 거의 나타나지 않았으며 또한 C₃, C_5가 α-chitin에서는 역평행 구조로서 2개의 signal로 나타나는데 본 실험에서는 β-chitin에서 보여지는 단일의 signal로 나타났다. 3. Chitosan을 0.2 M AcOH-0.1 M AcONa 용액에 녹여 30℃ 에서 점도를 측정한 결과 Mark-Houwink 식과 Rinaudo의 상수로 부터 [η]=1.424×10^(-5) M^(0.96)이었으며 분자량은 1.15×10^6이었다. 4. I.R spectrum을 측정한 결과 chitin은 1,653 ㎝^(-1)에서 amide I bend가 1,558㎝^(-1)에서 amide II bend가 나타났으며 chitosan은 1,601 ㎝^(-1)에서 1개의 넓은 bend가 생겼다. N-acetylchitosan의 경우 chitin과 마찬가지로 1,655 ㎝^(-1)에서 amide I bend가 1,560 ㎝^(-1)에서 amide II bend가 나타났다. 5. 아세틸 함량은 N-acetylchitosan 분말이 55.9%, acetylchitosan bead가 63.2%, N-ACF-1이 56%, N-ACF-2가 58.7% 이었다. 6. Chitin, chitosan 및 N-acetylchitosan을 formic acid에 용해하여 실온에서 시간에 따른 고유점도 변화를 관찰한 결과 chitosan이 chitin, N-acetylchitosan 보다 비교적 점도가 높았지만 3개 모두 시간이 지남에 따라 점도가 현저히 저하되어 25일째에는 chitosan이 14.39 ㎗/g, chitin이 1.31 ㎗/g, N-acetylchitosano 이 1.06 ㎗/g 이었다. In order to utilize the processing wastes of squid, chitosan was prepared by intermittent deacetylation treatment of β-chitin contained richly in the pen of squid. Acetylchitosan also was synthesized from squid pen chitosan with anhydrous acetic acid and their characteristics were investigated. The amounts of nitrogen and ash of squid pen chitosan were 5.8 ±0.2% and 0.2 ±0.03% respectively, the yield of squid pen chitosan was 25 ±3%, the degree of deacetylation was 92%, and the molecular weight was 1.15 ×10^6. Acetl contents of N-acetylchitosan powder, acetylchitosan bead, N-ACF-1 (N-acetylchitosan film-1) and N-ACF-2 (N-acetylchitosan film-2) were 55.9%, 63.2%, 56% and 58.7% respectively. Two major peaks, amide I (1,653 ㎝^(-1)) and Ⅱ (1,558 ㎝^(-1)) bent, on FT-IR spectra of the N-acetylchitosan from squid pen were almost similar to these of β-chitin. While there was a broad single peak at 1,601 ㎝^(-1) assigned to be an amide I bend in squid pen chitosan. The CP/MAS NMR specha of β-chitin, squid pen chitosan and Nacetylchitosan from squid pen showed a relative broad and single peak at 74 ppm assigned to fifth carbon (C-5) and third carbon (C-3). In case of β-chitin and N-acetylchitosan from squid pen, single peak at 74 ppm was showed as the same of β-chitin type.
최현미 ( Hyeon Mee Choi ),심창환 ( Chang Hwan Sim ),신태선 ( Tai Sun Shin ),빙동주 ( Dong Joo Bing ),전순실 ( Soon Sil Chun ) 한국식품영양학회 2011 韓國食品營養學會誌 Vol.24 No.3
In this study, certain quality characteristics of kimbugak samples prepared with 2 different puffing methods, microwave oven and frying, and containing different levels of sea tangle powder(0, 2, 4, and 6%), were examined according to mineral content, moisture content, color values, photographic images, hardness and sensory evaluations. The mineral contents of Porphyra yezoensis were: K, 2,612.400 ㎎; Ca, 116.970 ㎎; Na, 486.099 ㎎; Mg, 297.800 ㎎; Fe, 9.636 ㎎; Mn, 1.614 ㎎; and Cu, 0.973 ㎎. And those of the sea tangle powder were: K, 5,115.000 ㎎; Na, 1,848.931 ㎎; Ca, 763.030 ㎎; Mg, 525.700 ㎎; Fe, 15.125 ㎎; Zn, 4.639 ㎎; and Mn, 0.146 ㎎. Moisture content was not significantly different among the samples of kimbugak puffed by the microwave oven and frying. For the color of the kimbugak made with the microwave oven or frying, L values decreased as the level of sea tangle powder increased. Color a and b values were lowest in the control group. The color values(L, a, and b) of the kimbugak puffed by frying were higher than those made using the microwave oven. The hardness of the kimbugak samples puffed using the microwave oven were not significantly different according to levels of sea tangle powder, and in the kimbugak puffed by frying, the lowest hardness was in the sample with 6% sea tangle. In sensory evaluations, color and crispness were not significantly different among the samples. The flavor scores of the 4% and 6% samples puffed by frying were higher than those of the kimbugak samples puffed using the microwave oven. The overall acceptability of the kimbugak puffed by frying and containing 6% sea tangle powder was the highest. In conclusion, 4 and 6% sea tangle powder would be considered optimum amounts for kimbugak puffed by frying.
Choi, Suck-Chei,Kim, Beom-Su,Yoon, Kwon-Ha,Song, Moon-Young,Oh, Hyun-Mee,Han, Weon-Cheol,Kim, Tae-Hyeon,Kim, Eun-Cheol,Jun, Chang Duk The Korean Society for Integrative Biology 2002 Korean journal of biological sciences Vol.6 No.2
Nitric oxide has high affinity for iron, and thus it can cause intracellular iron loss. We tested the idea that intracellular iron can be the primary target of NO toxicity by comparing the signaling mechanisms involved in cell death caused by iron depletion and that caused by NO. Treatment of HL-60 cells with a NO donor, S-nitroso-N-acetyl-DL-penicillamine (SNAP), decreased the intracellular iron level rapidly as that observed with the iron chelator deferoxamine (DFO). Iron chelators such as DFO and mimosine could induce death of human leukemic HL-60 cells by a mechanism requiring activation of p38 kinase, c-Jun N-terminal kinase, caspase-3 and caspase-8. DFO and SNAP also caused release of cytochrome c from mitochondria. Inhibition of p38 kinase by a selective inhibitor, SB203580, abolished the NO and DFO-induced cell death, release of cytochrome c, and activation of caspase-3 and caspase-8, thus indicating that p38 kinase lies upstream in the cell death processes. In a parallel situation, the cells that are sensitive to NO showed similar sensitivity to DFO. Moreover, simultaneous addition of ferric citrate, an iron-containing compound, inhibited the SNAP and DFO-induced activation of caspases and also blocked the NO-mediated cell cycle arrest at $G_1$ phase. Collectively, our data implicate that the NO-induced cell death of tumor cells including HL-60 cells is mediated by depletion of iron and further suggest that activation of p38 kinase lies upstream of cytochrome c release and caspase activation involved in this apoptotic process.
Prenatal diagnosis by direct DNA analysis in facioscapulohumeral muscular dystrophy (FSHD) families
Choi, Soo-Kyung,Lee, Je-Hyeon,Kim, Bong-Yoon,Kim, Hyung-Goo,Cho, Eun-Hee,Ryu, Hyun-Mee,Kim, Young-Joe Korean Society of Medical Genetics 1998 대한의학유전학회지 Vol.2 No.1
Facioscapulohumeral muscular dystrophy (FSHD) is an autosomal dominant neuromuscular disorder which has been clinically shown to cause progressive weakness and result in atrophy of the facial muscles, shoulder girdle and upper arm muscles. The responsible gene for the FSHD has been located on chromosome 4q35-qter. The probes p13E-11 and pFR-1 detect DNA rearrangements associated with FSHD as under 28 kb DNA fragment in genomic southern analysis digested with EcoRI and the fragment contains 3.3 kb Kpn I tandem repeats. In this study, 4 fetuses with a family history of FSHD were analysed by genomic southern hybridization analysis with probes to determine whether they carried the deleted region. Of the 4 fetuses, three of them had mothers who were FSHD patients and the other one had a father affected with FSHD. After 10-11 weeks of gestation, we performed chorionic villi sampling and extracted DNA from uncultured and cultured tissue cells for the direct DNA analysis. The result of the southern analysis showed two fetuses having received about 15-18 kb of deleted genes from the father and the mother respectively, and found to be FSHD patients. The other two fetuses were shown to have two normal alleles from the parents and found to be normal. Two pregnancies which were determined to be normal were carried to term delivering two healthy babies.
Hot Water Extract of Leather Carp (Cyprinus carpio nudus) Improves Exercise Performance in Mice
Gong-Hyeon Lee,Dicky Harwanto,Sun-Mee Park,Jae-Suk Choi,Mi-Ryung Kim,Yong-Ki Hong 한국식품영양과학회 2015 Preventive Nutrition and Food Science Vol.20 No.4
The hot water extract of leather carp (Cyprinus carpio nudus) has been used as a nourishing tonic soup and as an aid for recovery from physical fatigue. In this study, we investigated the effect of leather carp extract on exercise performance in mice. Swimming endurance and forelimb grip strength were assessed following oral administration of the extract (once per day for 7 days) at a dose of 0.5 mg/10 L/g body weight. After 7 days, mice given the leather carp extract had significantly greater swimming endurance [105±18 s (P<0.05); 52% longer than day 0] and forelimb grip strength [1.18±0.05 Newton (P<0.01); 17% greater than day 0]. The extract increased muscle mass, but had little effect on body weight. Following the swimming exercise, blood glucose, glutathione peroxidase, and superoxide dismutase levels in extract-fed mice were significantly higher (145%, 131%, and 106%, respectively) than in the saline control group. Blood levels of high-density lipoprotein cholesterol were also significantly increased (128%) in mice given the extract compared to the controls. These results suggest that leather carp extract can improve physical exercise performance and prevent oxidative stress caused by exhaustive workouts.