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Lee, In Hwan,Ahn, Byungjun,Lee, Jeong Min,Lee, Chang Soo,Jung, Yongwon The Royal Society of Chemistry 2015 The Analyst Vol.140 No.10
<P>Water-soluble fluorescent silver nanoclusters (NCs) formed on biomolecule ligands have been extensively studied due to their great potential as new biocompatible fluorescent materials for biosensors. As synthetic ligands, proteins in particular can provide unique structures and functions to the assembled fluorescent silver clusters. A key challenge, however, is to develop appropriate protein ligands and synthetic approaches for cluster formation, especially using native aqueous solutions, to fully preserve the valuable properties of the protein templates. Here we report a human ferritin-templated synthesis of fluorescent silver NCs under neutral aqueous buffer conditions. The unique metal binding property of ferritin and an optimized silver ion reduction allowed us to produce highly stable fluorescent silver NCs that are steadily assembled in the cage-like ferritin proteins. The fluorescent clusters were also successfully assembled on genetically engineered ferritin with antibody-binding protein G. The resulting protein G-ferritin-silver NC complex fully retained the ferritin structure as well as the antibody binding ability. The present silver nanoclusters on ferritin (Ft-Ag NCs) also showed highly specific Cu<SUP>2+</SUP>-induced fluorescence quenching. By exploiting the large but stable nature of ferritin, we fabricated a highly robust and porous hydrogel sensor system for rapid Cu<SUP>2+</SUP> detection, where the Ft-Ag NCs were stably encapsulated in surface-bound hydrogels with large pore sizes. Our Ft-Ag NCs that are formed under native aqueous conditions will have great potential as a new fluorescent material with the high structural and functional diversities of ferritin.</P> <P>Graphic Abstract</P><P>Highly stable and copper-responsive fluorescent silver nanoclusters were assembled on human ferritin and retained the structure and function of the ferritin template as well as the fused binding protein. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4an02400a'> </P>
불법ㆍ불건전 인터넷 광고에 대한 매체의 책임 : 광고매개자인 인터넷 포털의 책임을 중심으로
이병준 한국정보사회진흥원 2009 정보화정책 Vol.16 No.1
인터넷 포털과 관련된 불법광고의 문제들은 대부분 기존의 광고관련 법률을 통하여 해결될 수 있으나, 새로운 현상에 대하여 구체적인 기준을 판례가 찾고 있는 과정에 있다. 본 논문에서는 이와 관련하여 두 가지 흥미로운 판결들을 살펴보았다. 첫째로 검색서비스와 관련하여 불법광고가 매개되는 경우에 관한 판결에서 법원은 불법광고에 대한 인식이 없는 상황에서는 일반적인 모니터링 의무가 존재하지 않고, 피해자의 신고가 있더라도 불법성이 명백하고 차단조치가 경제적, 기술적으로 가능한 경우 최소한도로 이루어져야 한다는 기본적 입장을 확인하였다. 검색서비스는 공공성을 지니기 때문에 정보의 차단이 지나쳐서는 안 된다는 점에서 차단의무를 조심스럽게 인정하는 것은 타당하다. 둘째는 인터넷 포털이 통신판매중개업자로서 통신판매업자의 불법광고에 대하여 책임을 물을 수 있는지에 관하여 문제가 되었다. 표시광고법 위반으로 행정적 제재의 대상이 되는 본 판결의 경우에는 소비자보호의 측면도 있지만, 공정한 거래질서 확립이라는 측면도 존재하므로 좀 더 객관적인 시각에서 광고주의 주체성을 판단해야 한다. Most illegal ad problems related to Internet portals can be solved through existing advertising-related laws, but for some new phenomena, concrete standards have not been established yet. So this paper examined two interesting judicial cases. The first case is about illegal ads carried through search services. The decision on the case was that a portal site does not have a duty to monitor illegal ads when it is not aware of them, and although there is a complaint against illegal ads, it is just enough for a portal to apply minimum ad filtering to its search service. The decision is reasonable because the search service is a sort of a public service and thus the blocking of information should not be excessive. The other case is about whether Internet portals as brokers can accuse online sellers of responsibility for illegal ads. The decision on the case was that online sellers are under administrative restrictions according to the Labeling and Advertising Law. It is needed to evaluate the subjectivity of an advertiser from a more objective point of view in order not only to protect consumers, but also to establish fair trade order.
Closed-loop Multi-rate Identification with zone-MPC using Constrained Subspace Method
ByungJun Park,Se-Kyu Oh,Jong Min Lee 제어로봇시스템학회 2017 제어로봇시스템학회 국제학술대회 논문집 Vol.2017 No.10
In many industrial processes, process variables have different sampling intervals and they are referred to as multi-rate (MR) systems. When these systems are controlled by model predictive control which is useful to control of constrained multivariable process, model mismatch can occur because of changing in process dynamics. Thus, identification of a new model is necessary. The new model should be identified in closed-loop if the process needs to comply particular operating range for product specification or safety requirement. In this paper, the re-identification problem of a MR system in a closed-loop with a MPC controller is addressed, considering the lifted system and known dynamic characteristics. In comparison with the existing approach, the proposed identification method provides more accurate and realistic model.
Nickel–copper hybrid electrodes self-adhered onto a silicon wafer by supersonic cold-spray
Lee, Jong-Gun,Kim, Do-Yeon,Kang, Byungjun,Kim, Donghwan,Song, Hee-eun,Kim, Jooyoung,Jung, Woonsuk,Lee, Dukhaeng,Al-Deyab, Salem S.,James, Scott C.,Yoon, Sam S. Elsevier 2015 Acta materialia Vol.93 No.-
<P><B>Abstract</B></P> <P>High-performance electrodes are fabricated through supersonic spraying of nickel and copper particles. These electrodes yield low specific resistivities, comparable to electrodes produced by screen-printed silver paste and light-induced plating. The appeal of this fabrication method is the low cost of copper and large area scalability of supersonic spray-coating techniques. The copper and nickel electrode was fabricated in the open air without any pre- or post-treatment. The spray-coated copper–nickel electrode was characterized by optical microscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction, and energy dispersive spectroscopy. Although both SEM and TEM images confirmed voids trapped between flattened particles in the fabricated electrode, this electrode’s resistivity was order 10<SUP>−6</SUP> Ωcm, which is comparable to the bulk value for pure copper.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
BWS based Interactive Streaming Service in T-DMB/WiBro Converged Mobile Environment
Byungjun Bae,Joungil Yun,Jong-Soo Lim,Yun-Jeong Song,Soo-In Lee 대한전자공학회 2009 ITC-CSCC :International Technical Conference on Ci Vol.2009 No.7
In this paper, we propose new BWS (Broadcasting WebSite) based interactive streaming service in consideration of the T-DMB/WiBro converged mobile environment. The BWS service specified in T-DMB is similar to web services through the internet. Therefore, it is expected to become one of useful data broadcasting services in T-DMB. We verify the proposed interactive BWS service supporting to link with the WiBro network by designing and implementing the test receiving platform and the return channel server. From the results, we present the technical possibility of new interactive broadcasting services on the move by utilizing the mobility of T-DMB and WiBro.
Lee, Junseok,Rhyou, Chanryeol,Kang, Byungjun,Lee, Hyungsuk Institute of Physics Publishing Ltd. 2017 Journal of Physics. D, Applied Physics Vol.50 No.16
<P>This paper describes continuously phase-modulated standing surface acoustic waves (CPM-SSAW) and its application for particle separation in multiple pressure nodes. A linear change of phase in CPM-SSAW applies a force to particles whose magnitude depends on their size and contrast factors. During continuous phase modulation, we demonstrate that particles with a target dimension are translated in the direction of moving pressure nodes, whereas smaller particles show oscillatory movements. The rate of phase modulation is optimized for separation of target particles from the relationship between mean particle velocity and period of oscillation. The developed technique is applied to separate particles of a target dimension from the particle mixture. Furthermore, we also demonstrate human keratinocyte cells can be separated in the cell and bead mixture. The separation technique is incorporated with a microfluidic channel spanning multiple pressure nodes, which is advantageous over separation in a single pressure node in terms of throughput.</P>
Multimodal System by Data Fusion and Synergetic Neural Network
Byungjun Son,Yillbyung Lee 한국지능시스템학회 2005 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.5 No.2
In this paper, we present the multimodal system based on the fusion of two user-friendly biometric modalities: Iris and Face. In order to reach robust identification and verification we are going to combine two different biometric features. we specifically apply 2-D discrete wavelet transform to extract the feature sets of low dimensionality from iris and face. And then to obtain Reduced Joint Feature Vector(RJFV) from these feature sets, Direct Linear Discriminant Analysis (DLDA) is used in our multimodal system. In addition, the Synergetic Neural Network(SNN) is used to obtain matching score of the preprocessed data. This system can operate in two modes: to identify a particular person or to verify a person's claimed identity. Our results for both cases show that the proposed method leads to a reliable person authentication system.