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야생참깨에서 agrobacterium rhizogenes 유도 모상근의 배양조건
Byung Ohg Ahn(安炳玉),Tetsuya Asamizu(淺水哲也),Kyo ji Yamada(山田恭司) 한국육종학회 1997 한국육종학회지 Vol.29 No.2
In order to investigate optimized culture conditions of hairy roots of Sesamum schinzianum (wild sesame) induced by infection of Agrobacterium rhizogenes, ATCC 15834 and MAFF03-01724 were cultured in various media, sucrose concentrations and pH levels. As the results, best growth condition of hairy roots induced by Agrobacterium rhizogenes strains was obtained in 1/2 MS medium free of hormone, 3% sucrose and pH 8.0. Hairy roots induced by Agrobacterium rhizogenes MAFF03-01724 showed better growth of extensive lateral root branches than that of ATCC 15834. In opine analysis, hairy roots induced by Agrobacterium rhizogenes ATCC 15834 was detected as agropine type while mikimopine in that of MAFF03-01724.
Isolation and Characterization of a Ds-tagged liguleless Mutant in Rice (Oryza sativa. L)
Byung-Ohg Ahn,지상혜,Doh-Won Yun,Hyeon-So Ji,Yong-Hwan Park,Sung-Han Park,Gi-Hwan Lee,Seok-Cheol Suh,Myung-Chul Lee 한국작물학회 2008 Journal of crop science and biotechnology Vol.11 No.4
A liguleless mutant, which showed complete loss of lamina joint region at the junction between leaf blade and leaf sheath, was isolated from a Ds insertional mutants derived from the source cultivar, Dongjin. This mutant could not affect other developmental patterns like phyllotaxis. Southern blot analysis, using GUS as a probe, revealed that the liguleless mutant contained three Ds copies transposed in the rice genome. Among the four genomic sequences flanking the Ds, one was mapped in the intergenic region (31661640 - 31661759), and the other two predicted a protein kinase domain (12098980 - 12098667) as an original insertion site within a starter line used for massive production of Ds insertional mutant lines. Another predicted and inserted in first exon of liguleless 1 protein (OsLG1) that was mapped in coding region (LOC_Os04g56170) of chromosome 4. RT-PCR revealed that the OsLG1 gene was not expressed liguleless mutants. Structure analysis of OsLG1 protein revealed that it predicted transcription factor with a highly conserved SBP domain consisting of 79 amino acids that overlapped a nuclear localization signal (NLS). RT-PCR revealed that OsLG1 is mainly expressed in vegetative organs.
Isolation and Characterization of a Ds-tagged liguleless Mutant in Rice (Oryza sativa. L)
Ahn, Byung-Ohg,Ji, Sang-Hye,Yun, Doh-Won,Ji, Hyeon-So,Park, Yong-Hwan,Park, Sung-Han,Lee, Gi-Hwan,Suh, Seok-Cheol,Lee, Myung-Chul The Korean Society of Crop Science 2008 Journal of crop science and biotechnology Vol.11 No.4
A liguleless mutant, which showed complete loss of lamina joint region at the junction between leaf blade and leaf sheath, was isolated from a Ds insertional mutants derived from the source cultivar, Dongjin. This mutant could not affect other developmental patterns like phyllotaxis. Southern blot analysis, using GUS as a probe, revealed that the liguleless mutant contained three Ds copies transposed in the rice genome. Among the four genomic sequences flanking the Ds, one was mapped in the intergenic region (31661640 - 31661759), and the other two predicted a protein kinase domain (12098980 - 12098667) as an original insertion site within a starter line used for massive production of Ds insertional mutant lines. Another predicted and inserted in first exon of liguleless 1 protein (OsLG1) that was mapped in coding region (LOC_Os04g56170) of chromosome 4. RT-PCR revealed that the OsLG1 gene was not expressed liguleless mutants. Structure analysis of OsLG1 protein revealed that it predicted transcription factor with a highly conserved SBP domain consisting of 79 amino acids that overlapped a nuclear localization signal (NLS). RT-PCR revealed that OsLG1 is mainly expressed in vegetative organs.