12-O-Tetradecanoylphorbol-13-Acetate에 의한 HL-60 세포 분화유도중 Vimentin 유전자 전사조절에 대한 AP-1의 역할

임규,김진희,권도원,김승민,이명선,윤경아,손미영,박종일,윤완희,황병두 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

Purpose: To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay. Materials and Methods: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics in a humidified 5% CO_2 at 37℃. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. EcoRI fragment of pVIM-GEM was used as probe for vimentin mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA probe(Upper strand, 5'-CGCTTGATGAGTCAGCCG- 3') for AP-1 binding activity was mixed with nuclear extracts in a 20 μl reaction volume containing 300 mM KCI, 60 mM HEPES, pH 7.9, 25 mM MgCI_2, 1 mM EDTA, 1 mM DTT, 60% glycerol, and 2 ㎍ of poly[dI-dC]. Results: TPA increased vimentin mRNA levels, with maximal stimulation reached at 24 hr. The level of vimentin mRNA was induced in proportion to the concentration of TPA. TPA-induced vimentin mRNA was almost reduced by actinomycin-D pretreatment. TPA-induced stimulation of vimentin gene was completely reduced by staurosporin pretreatment. In DNA mobility shift assay, AP-1 newly appeared at 24 hr during TPA-induced differentiation and was almost not detected after the pretreatment of staurosporin. Conclusions: These results suggest that the induction of vimentin mRNA during TPA-dependent differentiation in HL-60 cells may be mediated by protein kinases C signal transduction and AP-1 is important to transcriptional regulation.

12-O-Tetradecanoylphorbol-13-Acetate에 의한 HL-60 세포 분화유도중 Vimentin 유전자 전사조절에 대한 AP-1의 역할

임규,김진희,권도원,김승민,이명선,윤경아,손미영,박종일,윤완희,황병두 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Purpose: To gain insight on the role of AP-1 in transcriptional regulation of vimentin gene during differentiation of HL-60 cells by 12-O-tetradecanoylphorbol-13-acetate (TPA), the levels of vimentin mRNA and AP-1 have been investigated with Northern blot hybridization and DNA mobility shift assay. Materials and Methods: HL-60 cells were grown in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum and antibiotics in a humidified 5% CO_(2) at 37°C. Total RNA was prepared by a modification of the method of Karlinsey et al. Northern blot hybridization was performed by the method of Virca et al. EcoRI fragment of pVIM-GEM was used as probe for vimentin mRNA. DNA mobility shift assay was performed by the method of Lim et al. End labeled DNA probe(Upper strand, 5'-CGCTTGATGAGTCAGCCG- 3') for AP-1 binding activity was mixed with nuclear extracts in a 20 μl reaction volume containing 300 mM KC1, 60 mM HEPES, pH 7.9, 25 mM MgCl_(2), 1 mM EDTA, 1 mM DTT, 60% glycerol, and 2μg of poly[dI-dC]. Results: TPA increased vimentin mRNA levels, with maximal stimulation reached at 24 hr. The level of vimentin mRNA was induced in proportion to the concentration of TPA. TPA-induced vimentin mRNA was almost reduced by actinomycin-D pretreatment. TPA-induced stimulation of vimentin gene was completely reduced by staurosporin pretreatment. In DNA mobility shift assay, AP-1 newly appeared at 24 hr during TPA-induced differentiation and was almost not detected after the pretreatment of staurosporin. Conclusions: These results suggest that the induction of vimentin mRNA during TPA-dependent differentiation in HL-60 cells may be mediated by protein kinases C signal transduction and AP-1 is important to transcriptional regulation.

심미성 수복재의 변연누출에 관한 비교 연구

임선희,임호남,최부병 慶熙大學校 齒科大學 1993 慶熙齒大論文集 Vol.15 No.1

This study evaluated the in vitro early marginal seal effectiveness in class V restorations of glass ionomer cement with or without dentin treatment, sandwich technique, and composite resin with several kinds of dentin bonding agents. The preparations were as uniformly as possible, with depth 1.5 mm, size 4 X 3mm. The one kind of glass ionomer cement for each type, 40% polyacrylic acid for dentin treatment, one kind of composite resin and three commercial dentin bonding agents were tested. Four groups were restored with only glass ionomer cement to evaluate the effect of dentin treatment and the type of glass ionomer on marginal leakage. Two groups were filled with composite resin with glass ionomer base by means of sandwich technique. And three groups were restored wih three commercial dentin bonding agent(Gluma�, Scotchbond 2�, and Tenure) and composite resin. The one that was filled with composite resin without use of dentin bonding agent was used as control group. Ten experimental groups including control group were established and each group had ten cavities. Eight of ten cavities of each group were used for marginal leakage test and two of ten, for investigation of marginal gap. Immediately after being restored, all of specimens were thermocycled 100 times through water bath of 5 ± It and 55 ± It for 38 sec in each bath. Then, marginal leakage of them were estimated by dye penetration method and scanning electron micrographs were taken to evaluate the marginal gap between dentin and restorations at X 1,000 magnification. On the summary of this study, the conclusions were as follows 1. Generally, more marginal leakage at the gingival margins was investigated than at the occlusal margin. While the groups filled with composite resin with dentin bonding agents showed less marginal leakage at occlusal margin, the groups restored with glass ionomer cement had the lower marginal leakage score at gingival margin. 2 When glass ionomer/composite resin sandwich restorations were compared with other restorations, they had least margial leakage at gingival margin. 3. Types of glass ionomer cement and dentin treatment did not affect on the marginal leakage at occlusal margin. At gingival margin, marginal leakage of light curing glass ionomer cement with dentin treat-merit showed significantly higher marginal leakage and wider marginal gap was seen in light curing glass ionomer cement than in chemical curing glass ionomer cement. 4. Though Tenure� exibited significantly less marginal leakage than Gluma� and Scotchbond 20, all of three dentin bonding agents seldom made wide marginal gap equally.

TPM 우수기업을 위한 KS A ISO 2859-1과 KS A 3109와의 비교 분석

임재근,조병선,정수일 대한설비관리학회 2004 대한설비관리학회지 Vol.9 No.4

The growing importance of the product quality of the manufacturing industry is becoming more and more apparent as we enter the end of faster and deeper globalization trend. At the same time, among the various factors affecting the quality of the product, the importance of the production equipment is ever increasing. As a result, the TPM activity in Korean industries is getting increasing recognition and is being implemented with faster phase than ever. The companies with superior product quality are heavily involved with TPM activities and the product qualities of these companies are definitely better than the others. When we distinguish the product quality process into 3 steps, i.e. quality secure-quality confirm-quality guarantee, of course the 1st step of secure is the most important, but also the 2nd step of onfirm is one of the most important step. As well in mass production environment, sampling inspection is more desirable than the 100 % inspection procedure. As a part of globalization trend, KS system is also being revised and reestablished based on ISO, IEC, etc. *which are based on international standard. Conventional KS sampling inspection standards were in many areas quite different from ISO sampling inspection standards, only KS A 3102, 3104, 3151 are left and the rest be-come obsolete, and even the ones that are still around are planned to the gone step by step. It has been already 3 years since the new KS A ISO 2859-0--3 sampling inspection process has been established which the abolition of the popular KS A 3101, KS A 3105, KS A 3109, yet the implementation rate is very slow. This study will attempt to compare the old KS A 3109 with new KS A ISO 2859-0~3, and try to understand the difference as that the new standard can be easily understood and used widely among companies, by using examples. Our attempt is to help implement with the companies with active TPM involvement but the final result can be spread among other companies as well in the near future.

상사댐의 냉수관개가 수도 생육 및 수량에 미치는 영향

임준택,권병선 順天大學校 1997 論文集 Vol.16 No.1

냉수관개에 의한 수도생육 및 수량의 감소정도를 구명하기 위해 실시한 연구의 결과는 다음과 같다. 1) 냉수답과 온수답에서의 분얼수의 차이는 생육초기에는 커서 냉수답에서 평균적으로 35%정도의 감소를 보였으나 생육후기로 갈수록 차이가 없었고 오히려 냉수답에서 많은 분얼수를 보였다. 2) 냉수처리에 의한 초장은 생육초기에는 차이가 없었으나 생육중반에는 그 감소정도가 26%로 높았고 생육후기로 갈수록 낮아졌는데 그 크기는 19%정도였다. 3) 수온처리에 따라 고사엽수는 발생하지 않았고 생육초기에 적고정도에서 차이를 보였으나 그 정도는 미미한 정도였다. 4) 냉수답에서의 출수는 온수답에 비하여 대략 1주일정도 지연되었다. 5) 수확시 냉수답에서 평균 74.2㎝의 간장을 보여 온수답의 78.1㎝에 비해 약 5%의 단축을 보였다. 6) 냉수답과 온수답은 수장에서 각각 17.3과 19.2㎝를 보여 냉수에 의한 수장단축율은 12.2%였다. 7) 냉수답과 온수답은 주당수수에서 각각 17.2와 15.9개를 보여 냉수처리에 따라 주당수수는 오히려 증가하는 경향이었다. 8) 수당 영화수는 냉수답과 온수답이 각각 67.6개와 82.2개를 보여 냉수에 의한 수당 영화수의 감소율은 17.8%이었다. 9) 냉수답의 등숙율은 평균 72.2%로 온수답의 등숙율 78.1%보다 5.9% 낮은 경이었다. 10) 냉수답의 정조수량은 평균 545.2㎏/10a로 온수답의 평균 713.5㎏/10a보다 168.2㎏/10a정도 낮은 수준이었으며 냉수에 의한 수량감소가 적은 품종은 동진벼나 만금벼이었다. 냉수에 의한 수량감소는 간장의 감소와 등숙율의 감소에서 기인한다 할 수 있었다. This study was conducted to determine the effects of irrigating cold water on the growth and yield of rice. Rice plants were grown by irrigating water with two different temperatures, that is, irrigating cold water and warm water. In the condition of irrigation cold water, water from lake Sangsa of temperature from 11 to 20℃ was irrigated directly. In irrigating warm water, the same source of water but with 5-10℃ higher in water temperature was irrigated. Water temperature was increased by storing the water for several hours in a pond with a size of 14m in width, 25m in length and 30cm in depth. Rice plants were transplanted on June 15, and plant height and number of tillers per hill were measured nine times with the interval of 7 days from June 30. Heading date, and culm length, panicle length, yield and yield components were observed at harvest date. Water temperatures were monitored automatically at a interval of 1 hour from June 30 to Sept. 28. The results were summarized as follows. Number of tillers per hill appeared to be more than 30% lower in cold water treatment at the growing period up to July 14, but at the later growth stage, cold water treatment showed slightly higher value of number of tillers per hill compared with warm water treatment. There was no significant difference in plant height between treatments at the sampling date of June 30. From the sampling date of July 7, plant hight significantly decreased up to by 26%, but at the last sampling date of Aug. 25, the difference became smaller by 19%, compared with that of warm water treatment. The heading date of cold water treatment delayed about 7 days. Culm and panicle length in cold water treatment decreased 5% and 12.2%, respectively, compared with warm water treatment. Number of panicles per hill were 17.2 in cold water treatment and 15.9 in warm water treatment, so that cold water treatment showed higher value in number of panicles than that of warm water treatment. Number of spikelets per panicle in cold and warm water treatment were 67.6 and 82.2, respectively, so that cold water treatment showed 17.8% lower value in number of spikelets compared with warm water treatment. Percent of filled spikelets in cold water treatment was 72.2%, which was 5.9% lower than 78.1% of warm water treatment. Rough rice yield in cold water treatment were 545.2㎏/10a in average, which was 168.2㎏/10a lower than 713.5㎏/10a of warm water treatment. Decrease in yield under cold water irrigation mainly appeared to come from the decrease in culm length and percentage of filled spikelets.

퇴비 및 액비 시용에 따른 수수×수단그라스와 이탈리안라이그라스 작부체계의 수량과 양분흡수량 및 토양 유효 질소 및 인 변화

임상선,이광승,전병준,이세인 全南大學校 農業科學技術硏究所 2011 農業科學技術硏究 Vol.46 No.-

This study was conducted to investigate productivity and nutrients uptake of Sorghum×Sudan grass (SSG) and Italian ryegrass (IRG) cropping system with cattle manure compost and liquid pig manure application, respectively. Changes in mineral N and available P concentration before and after each cropping were also examined. Dry matter yield ranged from 2.12 to 2.86 ton ha-1 for SSG and from 3.57 to 6.08 ton ha-1 for IRG. Forage productivity observed in this study was substantially lower than other studies probably due both to low precipitation during cropping season and to soil characteristics; lower pH (<5) and available P concentration (ranged from 98.6 to 174.8) than the soils used in the previous studies. The uptake of N and P also showed very similar pattern to dry matter yield; IRG had higher nutrients uptake than SSG. According to nutrient balance analysis, 76.4% of N and 76.9% of P2O5 applied to the fields (161 kg N ha-1 and 265 kg P2O5 ha-1) were not assimilated by SSG. For IRG, 50.2% (133 kg N ha-1) of N applied (265 kg N ha-1) was not taken up; meanwhile, the amount of P2O5 assimilated by IRG was 75 kg P2O5 ha-1, and this was 49 kg P2O5 ha-1 more than that of applied P2O5 (26 kg P2O5 ha-1) as liquid manure, indicating additional P uptake from the soils. Accordingly, mineral N (NH4 + and NO3 -) and available P concentrations of the soils increased after SSG harvest as compared with the soils before cultivation, but they decreased after IRG cultivation. Such differences in nutrient uptake by plant and soil nutrient availability could be attributed to the contrasting availability of nutrients in the compost and liquid manure. Organic forms of nutrients included in the compost is likely to be released slowly by decomposition of organic matter and thus the nutrients released could be utilized by the next crop (IRG) rather than by the crop (SSG) to which compost was applied. On the other hand, as most nutrients in liquid manure is readily available, N and P in the liquid manure could be assimilated by IRG. Therefore, it might be necessary to consider both compost (slowly available) and liquid manure (rapidly available) to achieve a goal production of forage.

All-Trans Retinoic Acid에 의한 HL-60 세포 분화유도중 H2B Histone, c-Myc 및 DNA Topoisomerase I 발현에 관한 연구

임규,박정동,최병한,이용진,김계영,이명선,장은미,김삼용,권기량,곽상태,황병두 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

Effects of all-trans retinoic acid(retinoic acid) on DNA replication, H2B histone and DNA topoisopmerase I(Topo I) gene expression have been investigated in human promyelocytic leukemia cell line HL-60. DNA synthesis decreased at 24 hours after exposure of retinoic acid in the HL-60 cells. H2B histone mRNA rapidly reduced at 48 hours and Topo I and c-myc mRNA at 24 hours in retinoic acid-exposured HL-60 cells, respectively. The levels of c-myc, H2B histone and Topo I gene expression were reduced in proportion to the concentration of retinoic acid. The H2B histone mRNA in retinoic acid-exposured cells was elevated by cycloheximide treatment, while the level of Topo I mRNA was constant. These results suggest that regulations of H2B histone, c-myc and Topo I gene expression are different from one another, and repression of Topo I gene is closely correlated with c-myc gene during retinoic acid-induced differentiation of HL-60 cells.

남부 지역에서의 유자 재배 및 출하실태

권병선,임준택 順天大學校 1999 論文集 Vol.18 No.1

남부지역의 유자 재배 농가의 재배실태를 파악하여 유자 시험 연구의 기초자료로 활용코자 1998년에 남부 유자 주산지인 전남 고흥, 여천, 경남 남해의 3개군 90 농가를 대상으로 일반현황, 재배현황, 출하 등에 대한 실태 조사 결과를 요약하면 다음과 같다. 1. 전국적인 유자 생산량은 1985년도에 1,543M/T 이였던 것이 1998년도에는 19,826M/T으로 증가하였고 유자 재배 주산지인 고흥, 남해, 여천군도 1995년도 5,853M/T 이였던 것이 1998년도에는 7,345M/T으로 증가하였다. 2. 재배규모로는 200주 미만의 재배농가가 여천군이 54%, 남해군은 35%, 고흥군은 20%로서 여천군이 가장 영세하였다. 3. 방풍림 설치로 남해군이 17.0%, 고흥군이 8.5%, 여천군이 1.8%로서 83% 이상이 방풍림의 설치가 없이 재배하고 있었다. 4. 유자의 정지 전정의 최초 시기는 재식 후 4년 이후에 시작하는 것으로서 고흥군은 82%, 여천군은 78%, 남해군은 73%를 점유하였다. 5. 시비전에 깊이갈이 하는것은 2년에 1회가 56%로 많았고 다음으로는 1년에 1회가 15% 였으며 3년에 1회는 8%로서 전체 79%가 깊이갈이를 하고 있었다. 6. 2월하순, 5월하순, 8월하순에 표준시비량(㎏/10a) 보다 3요소를 더 많이 시비하였다. 7. 유자 재배 농가는 78%정도로 관수를 하고 있었으며 그 중에서 여름과 겨울철에 3회이상 관수를 하며 사질토에서는 1회에 15톤 정도로 3일 간격으로 점질토에서는 1회에 50톤 정도로 10일 간격으로 관수하고 있었다. 8. 유자 출하는 인근시에 출하 하는것이 46%, 농협으로 출하 하는것이 25%로 높았고 포전 출하도 15%나 되었다. 유자차 출하는 유자차의 규격을 1.5㎏, 3.0㎏의 유리병으로 상품화하여 서울 가락동 시장에 50%, 부산 유통업체에 20%, 인근지역에 30% 정도 출하하고 있었다. Some investigations were carried to know the cultivating and shipment status at three cultivated region for citron in southern region of Korea. The results are as follows: The cultivation and production of citron were practically increased every year. Farm size which had petty farms less than 200 plants was with 54% in Yeochon-Gun, 35% in Namhae-Gun and 20% in Goheung-Gen region. Fewer farmers plants the shelter belt with 1.8% in Yeochon-Gun, 17.0% in Namhae-Gun and 8.5% in Goheung-Gun region. First time of pruning and bending were in the later four years plant with 78% in Yeochon-Gun, 73% in Namhae-Gun and 82% in Goheung-Gun region. The deep tillage before the ferrilizing were once per two years with 50%, once per a year with 15% and once per three years with 8%. Standard fertilizer level was Most farmers fertilize with a large quantity of fertilizer in late in Feb., May and Aug. 78% of the farmers of citron cultivation irrigated with 15-20 to per once in 10a by interval of three days at sandy loam while clay loam irrigated with 50 ton per once in 10a by interval of ten days. Citron were sent out for buying in the field with 15%, public sale in agricultural association with 25%, public sale in horticultural association with 6%, agricultural and fishery marketing corporation in Seoul with 8% and near city with 46%. Citron tea were for sale with standardized goods of 1.5 or 3.0㎏ to the place of shipment in Seoul, Pusan and near city.

SA-ASTM 주사기법에 의한 악취발생량 평가 및 무취공기 표준화에 관한 연구

김선태,배병욱,김학민,임경묵 대전대학교 산업기술연구소 1999 산업기술연구소 論文集 Vol.10 No.1

New methodology to evaluate the complex odor phenomenon was investigated, as called SA-ASTM(Sensor Aided ASTM ) syringe method. Specially we studied on the standardization of non-odorous air which is very important procedure in measuring odorous gas including our SA-ASTM syringe method. We examined the odor sensor value of standard hydrogen sulfide gas and non-odorous air made with ambient and cylinder air. The decision coefficient between H2S concentration and odor sensor value showed 0.992, and correlation formula between olfactory odor degree(Y) and odor sensor value(X) was Y =0.0039log(X) -0.4129. Also, it appeared that it was not appropriate to use ambient air itself because the odor level of ambient air varies with time, site, and situation. Instead we concluded that it was very effective for making the non-odorous air to use the cylinder air with traps which are composed of water, vacant tube, silicagel, charcoal vessels. The analysis results of odor samples by SA-ASTM syringe method using non-odorous air of this study explained the odor threshold and odor intensity well, which is the basic characteristics of odor and necessary data to use the odor dispersion model.

女學生의 體格과 體力의 關聯에 關한 硏究

신순호,임병규 圓光大學校附設 生活體育硏究所 1993 生活體育硏究 Vol.4 No.-

The purpose of this study was to survey in the character of the physical development, growth and to change of the related elements of the physical development with increased age(9 - 14). Eight hundred fiften girls,ranging from 9 to 14 years of age, were used in this study. They were students of elementary and junior high school in Chollabuk-Do. Five physique items, 14 physical fitness items. Test were administered in March to May, 1992. The result were as follows ; 1. For the characteristics of the physical growth and development in youth girl students : In the character of the physical growth and development in the presence, muscular strength was the development of the same level as with growth of the physique elements. But the other elements were not so level as with growth of the physique elements. 2. For the correlation between physique and physical fitness in youth girl students : In the correlation between body length,body wide & volume and muscular strength was the highest(r=0.451 - 0.719, p<0.01) and between body wide & volume,skinfold thickness and muscular strength the higher(r=0.209 - 0.576, p<0.05), but the clear change of that correlation did not show in youth girl students. 3. For the degree of contribution of the physique elements to physical fitness elements in youth girl students : In the degree of contribution of body length,body wide & volume elements to physical fitness elements was the highest(20.34 - 51.70%), but physique elements to agility and balance elements were the lowest(about 2.0%) in youth girl students.