늑막삼출 유무에 따른 마이코플라즈마 폐렴의 임상 양상의 비교

변신연 ( Shin Yun Byun ),배윤진 ( Yun Jin Bae ),유재호 ( Jae Ho Yoo ),정진아 ( Jin A Jung ) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 2006 소아알레르기 및 호흡기학회지 Vol.16 No.4

목적: Mycoplasma pneumoniae는 학동기 소아에서 호흡기 감염의 주된 원인균이며, 마이코플라즈마 폐렴의 호흡기 합병증으로는 무기폐, 늑막 삼출, 폐농양, 폐기종, 기관지 확장증 등이 있다. 이번 연구에서는 마이코플라즈마 폐렴에서 늑막 삼출 유무에 따른 임상 양상의 특징을 비교해보고자 하였다. 방법: 2000년 1월부터 2004년 12월까지 동아대학교의료원 소아과에 입원한 마이코플라즈마 폐렴 환자 210명을 대상으로 입원 기록을 후향적으로 비교분석하였다. 마이코플라즈마 폐렴의 진단기준은 항 마이코플라즈마 항체역가가 1:320 이상인 경우로 하였으며 늑막 삼출을 동반한 경우(A군)와 동반하지 않은 경우(B군)로 나누어 성별, 연령, 발생시기, 증상, 이학적 소견, 검사 소견, 흉부 방사선 소견을 비교하였다. 결과: A군과 B군 간의 연령, 성별, 발생시기, 증상 및 청진 소견에서는 유의한 차이는 없었다. 발열기간과 입원기간은 A군이 B군보다 의미있게 길었다.(P<0.001) 적혈구 침강속도는 A군에서 49.6±32.9㎜/hr로, B군의 28.7±20.4㎜/hr보다 의미있게 높았으며(P=0.001), C-반응성 단백은 A군에서 23.0±60.4㎎/dL로, B군의 8.7±30.9㎎/dL보다 의미있게 높았고(P=0.004), SGOT와 SGPT는 A군이 각각 67±74.2 IU/L와 53.6±60.0 IU/L로, B군의 37.4±18.6 IU/L와 26.2±16.9 IU/L보다 의미있게 높았다. (P<0.001) 늑막 삼출 유무와 항 마이코플라즈마 항체역가 사이의 유의성은 없었다. 결론: 늑막 삼출이 동반된 마이코플라즈마 폐렴군에서 발열기간, 입원기간, 적혈구 침강속도, C-반응성 단백, SGOT, SGPT가 늑막 삼출이 동반되지 않은 군에 비해 유의하게 증가되어 있어, 이러한 검사 소견들을 통하여 치료 경과를 예측할 수 있을 것으로 생각한다. Purpose: Mycoplasma pneumoniae (M. pneumoniae) is a major cause of respiratory infections in school-aged children. Complications of M. pneumoniae pneumonia include atelectasis, pleural effusion, empyema, pneumothorax and bronchiectasis. We evaluated the clinical characteristics of M. pneumoniae pneumonia with pleural effusion. Methods: A total of 210 medical records of children who were admitted to the Dong-A University hospital due to M. pneumoniae pneumonia from 2000 to 2004 were retrospectively analyzed. Diagnosis of M. pneumoniae pneumonia was based on the single titer of antimycoplasmal antibody higher than 1:320. Enrolled children were divided into Group A (with pleural effusion) and Group B (without effusion). We analysed the differences between the two groups according to sex, age, onset, symptoms, clinical manifestations, laboratory findings and chest x-rays. Results: There were no significant differences in age, sex and clinical manifestations between the two groups. Group A had longer fever durations (9.3±7.8 days vs 5.0±3.7 days), and a longer duration of hospitalization (10.4±6.3 days vs 6.9±6.3 days) compare to Group B. Also, compared to the Group B, Group A had higher ESR (49.6±32.9㎜/hr vs 28.7±20.4㎜/hr), CRP (23.0±60.4㎎/dL vs 8.7±30.9㎎/dL), SGOT (67±74.2 IU/L vs 53.6±60.0 IU/L), SGPT (37.4±18.6 IU/L vs 26.2±16.9 IU/L). There was no significance between antimycoplasmal antibody titer and pleural effusion. Conclusion: This study shows that M. pneumoniae pneumonia with pleural effusion has a longer duration of fever and hospitalization, higher ESR, CRP, SGOT, SGPT compare to the M. pneumoniae pneumonia without pleural effusion. We conclude that these findings could be used as the prognostic factors in M. pneumoniae pneumonia with pleural effusion. [Pediatr Allergy Respir Dis(Korea) 2006;16:327-334]

Phorbaketal A stimulates osteoblast differentiation through TAZ mediated Runx2 activation

Byun, Mi Ran,Kim, A Rum,Hwang, Jun-Ha,Sung, Mi Kyung,Lee, Yeon Kyung,Hwang, Buyng Su,Rho, Jung-Rae,Hwang, Eun Sook,Hong, Jeong-Ho Elsevier 2012 FEBS letters Vol.586 No.8

<P><B>Highlights</B></P><P>► Phorbaketal A stimulates osteogenesis. ► Phorbaketal A increases TAZ-mediated osteoblast marker gene expression. ► ERK is an important kinase for phorbaketal A induced osteogenic differentiation. ► Phorbaketal A is suggested as a potential compound for stimulating bone formation.</P> <P><B>Abstract</B></P><P>Osteoporosis arises from an imbalance between osteoblastic bone formation and osteoclastic bone resorption. In this study, we screened molecules from marine natural products that stimulate osteoblast differentiation. We found that phorbaketal A significantly stimulates osteoblast differentiation in mesenchymal cells. Increased interaction of TAZ and Runx2 stimulated phorbaketal A-induced expression of osteoblastic marker genes. The activation of ERK was important for the stimulation of differentiation because an inhibitor of ERK blocked phorbaketal A-induced osteogenic differentiation. Taken together, the results showed that phorbaketal A stimulates TAZ-mediated osteoblast differentiation through the activation of ERK.</P><P><B>Structured summary of protein interactions</B></P><P><B>TAZ</B> physically interacts with <B>RUNX2</B> by pull down (View interaction)</P>

마우스에서 Bisphenol A의 아급성노출이 IgM-PFC 형성능과 비장세포 증식능에 미치는 영향

변정아,표명윤 한국환경독성학회 2003 환경독성보건학회지 Vol.18 No.3

To determine whether or not bisphenol A affects the immune system, female, ICR mice were treated bisphenol A(BPA) orally at the doses of 100, 500 and 1,000mg/kg for 30 consecutive days. Four days before enumerating plaque-forming cells(PFCs) mice were immunized intraperitoneally with sheep red blood cells(SRBCs). The spleen cellularity and PFC/spleen were significantly reduced by 30-day exposure to BPA(1,000mg/kg/day), but the PFC/10^(6) spleen cells was slightly decreased.. When splenocytes isolated from the mice exposed to BPA for 30days were cultured in the presence of LPS, Con A or PHA with IL-2, the lymphocyte proliferation ex vivo was not significantly suppressed by BPA. Our present results indicated that 30-day exposure of mice of BPA might have mild immunotoxic potential.

Bisphenol A의 급성노출이 마우스의 면역기능에 미치는 영향

표명윤(Myoung-Yun Pyo),변정아(Jung-A Byun) 大韓藥學會 2001 약학회지 Vol.45 No.1

In order to investigate the effects of bisphenol A (BPA) on immune system in mice we examined the various immunological parameters. After single oral adminstration of BPA to female ICR mice, the weights of bodies and lymphoid organs, splenic cellularity and hematological parameters were examined on day 2 and 7. Among them WBC and splenic cellularity were slightly decreased on day 2. To assess the effects of BPA on humoral immune responses, splenic IgM plaque forming cell (PFC) and serum IgM level were assayed. When BPA was administered after immunization with SRBC, but not before immunization, IgM level was also decreased on day 4 when high dose (2000mg/kg) of BPA was administrated after injection of OVA-antigen. The indexes of splenocyte proliferation (SP) to concanavalin A (Con A) and bacterial lipopolysaccharide (LPS) were measured in vitro by MTT assay. At low concentration BPA slightly increased splenocyte proliferation but at higher concentration it showed signficant inhibitory effects on cell proliferation Mitogen-stimulated SP was also determined with spleen cells from BPA treated mice. Con A-induced SP was slightly decreased and LPS-induced SP was eapecially inhibited at 1000mg/kg and 2000mg/kg of BPA. These results indicate that BPA is able to acutly evoke humoral and cell mediated immune suppression in mice.

난관수종액이 초기 임신 과정에 태반 세포 증식에 미치는 영향

김정욱,김계현,이지애,한정렬,최범채,변혜경,유근재,궁미경,조셉 힐 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.8

Objective : To assess the effect of hydrosalpingeal fluid on trophoblast cell proliferation and evaluate the cytokine level in hydrosalpingeal fluid Materials and Methods : Hydrosalpingeal fluid samples obtained from 10 women at the time of laparoscopic surgery. Trophoblast cell proliferation was assayed by a colorimetric immunoassay, based on the measurement of BrdU incorporation during DNA synthesis in response to hydrosalpingeal fluid and the levels of the Th-1 and Th-2 type cytokine determined by sandwich ELISA. Statistical analysis was performed by regression analysis and nonparametric testing(Mann-Whitney U test). Results : Samples from 7 out of 10 patients significantly suppressed trophoblast proliferation in a dose- dependent manner compared to controls(r=-0.0673, p$lt;0.05). IFN-γ and IL-6 were present in 2 out of 10 hydrosalpingeal fluid samples. IL-10 was not detected in all 10 samples. TNF-α, Th-1 type cytokine, was checked in all samples [median(range); 13.12 pg/ml(8.27 pg/ml~19.67 pg/ml)], and the mean concentration of TNF-α was significantly greater in group that suppressed trophoblast proliferation than in group that did not(14.74 pg/ml ± 1.05 pg/ml versus 9.33 pg/ml ± 0.55 pg/ml, p=0.0164). Conclusions : These findings suggest that hydrosalpingeal fluid may negatively affect trophoblast cell proliferation leading to implantation failure and also high level of the Th-1 type cytokine, TNF-α, in hydrosalpingeal fluid may be a potential mechanism for early pregnancy loss.

Rhizopus oligosporus에 의한 Oncom제조

박성오,한영란,변정아 서울여자대학교 자연과학연구소 1991 자연과학연구논문집 Vol.2 No.-

Peanut presscake powder with 1% different starch sources (tapioca, corn, potato) was used for preparation of Oncom and inoculated with Rhizopus oligosporus and incubated at 30℃ for 48 hours. During incubation, it was analyzed every 12 hours for the determination of moisture, reducing sugar, soluble sugar, total sugar, crude lipid, pH, crude protein, soluble nitrogen and total amino acids, and, for mineral determination, final product was used. Oncom prepared with peanut presscake as control (group Ⅰ), 1% tapioca (group Ⅱ), 1% corn starch (group Ⅲ) and 1% potato starch (group Ⅳ) added to peanut presscake were determined. The results of 48 hours incubation product were as follows : the moisture content for group Ⅰ,Ⅱ,Ⅲ and Ⅳ were 55.84, 53.50, 54.47 and 58.86 (wt%), respectively and its content was increased compared with unfermented peanut presscake. Reducing sugar content were 0.98, 1.06, 0.78 and 0.60(wt%), respectively and soluble sugar content were 1.06, 0.89, 1.05 and 0.99(wt%), respectively and it was increased slightly compared with unfermented peanut presscake. Total sugar content were 5.01, 4.54, 2.66 and 5.16(wt%), respectively and it was decreased greatly compared with unfermented one. Crude lipid content were 7.24, 7.24, 7.08 and 6.94(wt%), respectively and it was decreased slightly. The value of pH were 6.38, 6.31, 6.20 and 6.22, respectively and it was increased compared with unfermented one. The crude protein content were 5.25, 4.97, 5.99 and 5.31(wt%), respectively and it was almost same as unfermented one. The soluble nitrogen content were 26.0, 23.0, 25.0 and 20.0(mg%), respectively and it was increased almost 7 times. Total amino acids content were 1404.0, 1468.4, 1342.4 and 1377.4(mg%), respectively and it was increased very slightly. The minerals (K, Na, Ca, Cu, Mg, Mn, Fe, Zn) contents were determinted. The content of total sugar, K, Na, Mg, Mn were higher in Ⅳ group than any other groups. The content of reducing sugar, crude lipid, total amino acids were higher in Ⅱ group than any other groups. The content of soluble sugar, crude lipid, soluble nitrogen were higher in Ⅰ group than other groups and the content of crude protein in group Ⅲ were higher than other groups.

장소자산(場所資産)의 변용(變容) 양상(樣相)으로 본 ‘시코쿠헨로’

변정아 ( Byun Jung-a ) 한국일본근대학회 2019 일본근대학연구 Vol.0 No.65

본 연구는 ‘시코쿠헨로’가 가지고 있던 역사자원을 현재의 시코쿠의 관광콘텐츠로서 활용할 수 있는 장소자산의 변용에 주안점을 두고 분석하고자 한다. ‘시코쿠헨로 88개소’는 시코쿠 4개 현 전체를 관통하며 분포되어 있는 시코쿠 전체 자산이다. 따라서 4개현 지자체의 지역 정책 시스템 하에 지역 간 협의와 협력이 이루어져야 시코쿠 전체 장소자산으로서 효과를 상승시킬 것이다. ‘시코쿠헨로’는 일본 불교의 종류를 총 망라하고 있는 독특한 순례길이므로 대중은 종파에 얽매이지 않고 순례를 할 수 있다. 현재의 시코쿠헨로는 종교적 가치의 엄중함 없이 유연한 방식의 순례를 권장하기 때문에 국적, 성별, 연령에 상관없이 관광객 개인의 상황에 따라 자유롭게 순례 방법을 선택할 수 있다. 순례 행위 자체는 현재까지 전통으로서 명맥을 유지하지만 시간에 따라 형태, 의미, 목적은 다소 변용되었다. 따라서 시코쿠헨로도 시대에 맞게 종교적인 수행뿐만 아니라 병의 치유·공양 외에, 마음의 위안을 찾아 휴식과 건강증진, 등산이나 하이킹의 연장, 관광여행의 일환 등, 다양한 형태로 현대인의 힐링 여행으로서 재발견되며 관광콘텐츠로 부활하고 있다. This research focused on the change of place asset to utilize the historical resources of ‘Shikoku Henro’ as tour contents of present Shikoku. ’88 Places in Shikoku Henro’ are the asset of the whole Shikoku, distributed throughout 4 hyun of Shikoku. Therefore, the effect of the place asset of the entire Shikoku can be improved when regional agreement and cooperation is made under the regional policy system of the local governments of 4 hyun. ‘Shikoku Henro’ is a unique pilgrimage that encompasses the kinds of Japanese Buddhism, so the people can make a pilgrimage without being tied to a sect. Since current Shikoku Henro encourages a flexible way of pilgrimage without a strict religious value, visitors are free to choose how to make a pilgrimage regardless of their nationality, gender, or age. The pilgrimage act itself retains life as a tradition so far, however its form, meaning, and purpose have been somewhat changed along with time. Accordingly, Shikoku Henro is rediscovered as a healing journey of modern people in various ways such as relaxation, health promotion, extension of the mountain climbing and hiking, a part of travel, besides religious performance proper for Shikoku Henro Era, and is revived as a tourism content.

김달수의 「후예의 거리」 연구 - 도쿄의 모조도시인 경성에서 부유하는 지식인들-

변정아 ( Byun Jung-a ) 한국일본근대학회 2018 일본근대학연구 Vol.0 No.62

김달수의 최초의 장편이며 최초로 출판된 「후예의 거리」는 그의 경성 방문과「경성일보」기자의 경험을 토대로 한 작품이다. 특히, 「경성일보」의 사회부 기자를 했던 것은 김달수에게 있어 피지배자로서 당하는 동포들의 비참한 생활상과 일제 권력의 압박을 받으면서도 암암리에 활동하고 있는 항일 운동가들의 투쟁에 대해서도 직접 접할 수 있는 최적의 경험이었을 것이다. 이런 고국에서의 생활은 그가 조선인의 하나로서 스스로를 자각하게 하고, 작가로서 민족의식을 확립해가는 과정이 되었을 것이다. 짧은 기간이었지만 고국에서의 체험은「후예의 거리」의 주인공 ‘고창륜’이 경성에서의 생활과 그곳에서 만난 지식인들의 실상을 통해 민족의식을 자각하여 변해가는 과정을 그리고 있다. 일본제국주의는 조선을 식민화하면서 경성을 서양의 모조품인 일본 도쿄를 모방한 더 심각한 이중의 모조도시로 만들고 있었다. 한 거리에 근세와 근대가 뒤죽박죽인 모습이었고 일반 사람들은 이상과 현실, 신념과 행동이 어지럽게 뒤엉켜있었다. 이러한 이중적인 모조도시 경성이란 공간은 그 시대 지식인들의 굴욕과 모순된 조선의 현실을 부각시키고 있다. 김달수는 작품의 무대를 일본이 아닌 조선으로 옮겨와 고창륜의 시선으로 관찰되는 경성의 모습과 그가 만나는 조선 지식인들의 고민과 안주하지 못하는 삶을 통해 민족의식을 표출하고 있다. 김달수가 「후예의 거리」에서 고국을 잃어버린 고창륜을 통해 식민지지배의 현실, 그 중에서도 지식인들의 고뇌와 죽음을 두려워하지 않고 의연히 투쟁하는 독립운동에 대한 의지를 보여줌으로써 그 당시의 조선의 실상을 그리고 있다. Kim Dal Soo’s first feature and first published 「Descendant’s Street」is based on his visit to Kyungseong and his experience as the journalist of Kyungseong Ilbo. In particular, as a local news reporter of the Kyungseong Ilbo was optimum experience for Kim Dal Soo to be able to access miserable life of his compatriots and the struggles of anti-Japanese activists under the pressure of Japanese rule. Life in this home country would have been a process for him to recognize himself as one of the Joseon people and to establish a national consciousness as a writer. Although it was only a short period of time, the experience in the home country helped him to write the process of changing national consciousness of Ko Chang Ryun, the main character in 「Descendant’s Street」, through his life in Kyungseong and the realities of intellectuals who he met. Japanese imperialism had colonized Joseon, turning Kyungseong into doubled-imitation city by imitating Tokyo which already had imitated the West. The street was in a mess with modern and modern people and people were confused by ideals, realities, beliefs and actions. This dual imitation city of Kyungseong highlights the humiliation of intellectuals of the time and the reality of Joseon in contradiction. Kim Dal Soo is expressing his national consciousness by setting the stage of his work to Joseon, not Japan, through Ko Chang Ryun’s perspective of the Kyungseong life that cannot be settled down and concerns of the Joseon intellectuals who he meets. In 「Descendant’s Street」, Kim Dal Soo is showing the reality of colonization through Ko Chang Ryun, who has lost his homeland, especially by showing the intellectuals’ torment and their will to fight for independence without fear of death.

마우스에서 bisphenol A 노출로 인한 면역병리학적 연구

변정아(Jung A Byun),표명윤(Myoung Yun Pyo) 한국환경성돌연변이발암원학회 2002 한국환경성돌연변이·발암원학회지 Vol.22 No.4

Bisphenol A (BPA) is a monomer widely used in the manufacturing polycarbonate plastics or epoxy resin, and xenobiotics recently known as endocrine disrupting chemical. In this paper, to assess the effects of bisphenol A on immunopathological parameters (body weight, organ weight, hematological parameters, cellularity and surface marker) in mice, ICR female mice were orally exposed to BPA dissolved in olive oil as concentrations of 100, 500, 1000 mg/kg/day b.w. 5 days a week for 30 days (subacute exposure). Liver- and kidney- weight was significantly increased as dose-dependent manner, but body- , spleen- and thymus- weight didn't changed. In hematological parameters, WBC and MCHC were lowered but HCT and MCV were siginificantly enhanced. There was no significant differences in peritoneal macrophages number of the mice exposed to BPA.<br/> However, number of splenocytes of spleen, CD3+ and CD4+ cell in splenocytes, CD4+ and CD8+ cell in thymocytes were decreased at the mice subacutely exposed to BPA. In addition, BPA decreased expression of B7-1 and B7-2 on macrophages. Therefore, these results showed BPA may affect hematological parameters, cellularity<br/> and surface marker of immunocytes.

THE FIRST VERY LONG BASELINE INTERFEROMETRY IMAGE OF A 44 GHz METHANOL MASER WITH THE KVN AND VERA ARRAY (KaVA)

Matsumoto, Naoko,Hirota, Tomoya,Sugiyama, Koichiro,Kim, Kee-Tae,Kim, Mikyoung,Byun, Do-Young,Jung, Taehyun,Chibueze, James O.,Honma, Mareki,Kameya, Osamu,Kim, Jongsoo,Lyo, A-Ran,Motogi, Kazuhito,Oh, C IOP Publishing 2014 ASTROPHYSICAL JOURNAL LETTERS - Vol.789 No.1

<P>We have carried out the first very long baseline interferometry (VLBI) imaging of a 44 GHz class I methanol maser (70-61A(+)) associated with a millimeter core MM2 in a massive star-forming region IRAS 18151-1208 with KaVA (KVN and VERA Array), which is a newly combined array of KVN (Korean VLBI Network) and VERA (VLBI Exploration of Radio Astrometry). We have succeeded in imaging compact maser features with a synthesized beam size of 2.7 milliarcseconds x 1.5 milliarcseconds (mas). These features are detected at a limited number of baselines within the length of shorter than approximate to 650 km corresponding to 100 M. in the uv-coverage. The central velocity and the velocity width of the 44 GHz methanol maser are consistent with those of the quiescent gas rather than the outflow traced by the SiO thermal line. The minimum component size among the maser features is similar to 5 mas x 2 mas, which corresponds to the linear size of similar to 15 AU x 6 AU assuming a distance of 3 kpc. The brightness temperatures of these features range from similar to 3.5 x 10(8) to 1.0 x 10(10) K, which are higher than the estimated lower limit from a previous Very Large Array observation with the highest spatial resolution of similar to 50 mas. The 44 GHz class I methanol maser in IRAS 18151-1208 is found to be associated with the MM2 core, which is thought to be less evolved than another millimeter core MM1 associated with the 6.7 GHz class II methanol maser.</P>