Mancozeb이 마우스 비장세포의 IFN-$\gamma$생성능에 미치는 영향

표명윤,정애희,Pyo Myoung-Yun,Cheong Ae-Hee 환경독성보건학회 2005 환경독성보건학회지 Vol.20 No.3

Mancozeb (MCZ), a polymeric complex of zinr and manganese salts of ethylene bisthiocarbamate, is widely used in agriculture as fungicidel, insecticides, and herbicides. MCZ can be occupationally and environmentally exposed to human and has been reported to have detrimental effects on the reproductive system, but the toxicity of MCZ on immune responses has not been systematically investigated. We investigated the effects of MCZ exposure on the activities of murine splenocytes through evaluation of splenocytes cellularity and INF-$\gamma$ synthesis. Splenocytes were examned ex vivo from mice orally treated with various doEes of MCZ for 1 day (acute exposure, 2,100, 5,000, 10,000 mg/kg) or ior 5 consecutive days per week for 4 weeks (subacute exposure,250, 1,000, 1,500 mg/kg/day) fellowed by culture for 2 days in the presence of Con A or PHA plus IL-2. Splenocytes Iron naive mice were cultured with various concentration of MCZ (50, 500, 1,000 ng/ml) in the presence of Con A or PHA plus IL-2 for 2 days in vitro. IFN-$\gamma$ production was decreased with the in vitro exposure to all concentration of MCZ. The spleen cellularity and IFN -$\gamma$ production by splenocytes from MCZ -acutely and - subacutely exposured mice were decreased in comparision with that oi control group.

食用버섯類의 아미노酸에 關한 硏究

表明允(Myoung Yun Pyo),魯一協(Ihl Hyeob Ro) 韓國營養學會 1975 Journal of Nutrition and Health Vol.8 No.1

Bisphenol A의 급성노출이 마우스의 면역기능에 미치는 영향

표명윤(Myoung-Yun Pyo),변정아(Jung-A Byun) 大韓藥學會 2001 약학회지 Vol.45 No.1

In order to investigate the effects of bisphenol A (BPA) on immune system in mice we examined the various immunological parameters. After single oral adminstration of BPA to female ICR mice, the weights of bodies and lymphoid organs, splenic cellularity and hematological parameters were examined on day 2 and 7. Among them WBC and splenic cellularity were slightly decreased on day 2. To assess the effects of BPA on humoral immune responses, splenic IgM plaque forming cell (PFC) and serum IgM level were assayed. When BPA was administered after immunization with SRBC, but not before immunization, IgM level was also decreased on day 4 when high dose (2000mg/kg) of BPA was administrated after injection of OVA-antigen. The indexes of splenocyte proliferation (SP) to concanavalin A (Con A) and bacterial lipopolysaccharide (LPS) were measured in vitro by MTT assay. At low concentration BPA slightly increased splenocyte proliferation but at higher concentration it showed signficant inhibitory effects on cell proliferation Mitogen-stimulated SP was also determined with spleen cells from BPA treated mice. Con A-induced SP was slightly decreased and LPS-induced SP was eapecially inhibited at 1000mg/kg and 2000mg/kg of BPA. These results indicate that BPA is able to acutly evoke humoral and cell mediated immune suppression in mice.

Vinblastine과 Vincristine이 1차 및 2차 세포성 면역반응에 미치는 영향

표명윤(Myoung-Yun Pyo) 한국생약학회 1986 생약학회지 Vol.17 No.3

Effects of vinblastine(VLB) and vincristine(VCR) on cell-mediated immunity (CMI) were studied with the microcytotoxicity test (MCT) after normal or pre-sensitized Balb/c mice had been treated in vivo with a combination of two different doses of VLB or VCR(single dose of 20% and 60% LD_(50), i.p.) at different times (from day -6 to day +4) plus allo-transplantation antigen(allo-TA, cells from C3H mice at day 0). The results were that LD_(50) of VLB for female Balb/c mouse was 7.3㎎/㎏ body weight (i.p.) and LD_(50) of VCR was 4.3㎎/㎏ body weight and that VLB and VCR acted as immunosuppressive agents on the primary CMI when administered after allo-TA (antigen-drug-phase), but showed no effect when administered prior to allo-TA (drug-antigen-phase). Change of doses of VLB and VCR(20% I-D_(50), 60% LD_(50)) caused quantitative or qualitative variations in the immunomodulating effects of these two drugs. Neither VLB nor VCR had any immunomodulating effect on the secondary CMI. Lastly, the results support that the four parameters (type of drug, sensitization status, time of drug treatment in relation to antigen injection, and drug dosis) are significant for the effects of the VLB and VCR on the CMI, and that VLB and VCR may inhibit the proliferation of antigen-stimulated T effector lymphocytes but not memory-cytotoxic T lymphocytes.

In vitro에서 Bisphenol A 노출에 의한 세포성 면역반응의 변화

표명윤(Myoung-Yun Pyo) 대한약학회 2005 약학회지 Vol.49 No.1

ln order to investigate the effetcts of bisphenol A (BPA) on cell mediated immune reaction in vitro we examined the allogenic mixed lymphocyte reaction (MLR), splenocytes proliferation (SP) to T cell mitogens and IFN- γ production. Splenocytes of Balb/c mice (1.5×105 cells/well) were co-cultured with different numbers of mitomycin C-treated mature dentritic cells (DCs) in presence of BPA (25, 50, 100 ㎛) and (3H)thymidine incorporation (cpm) was measured by scintilation counting. Splenocytes (2×106 cells/well) were cultured with mitogens, Con A (2 ㎍/㎖), PHA (5 ㎍/㎖) and IL-2 (0.1 ㎍/㎖), or PMA (5 ng/㎖) and INO (1㎍/㎖) in presence of BPA (1, 10, 25, 50, 100 ㎛) and SP was assessed by MTT assay. IFN-γ levels in culture supernant were determined by ELISA. At low concentration, BPA slightly increased MLR, SP and IFN-γ levels, but at higher concentration it showed significant inhibitory effects on these immunological parameters. These results indicate that BPA is able to alternate cell-mediated immune reaction.

상황버섯 추출물이 정상 마우스와 cyclophosphamide 로 처리된 마우스의 체액성 면역기능에 미치는 영향

표명윤(Myoung Yun Pyo),현수미(Su Mi Hyun),양기숙(Ki Sook Yang) 한국응용약물학회 2001 Biomolecules & Therapeutics(구 응용약물학회지) Vol.9 No.3

N/A Phellinus linteus (PL)-hot water extract (PL-W) or- methanol extract (PL-M) was orally administered alone (single dose of 400, 800, 1600 mg/kg; 800 mg/kg/day for 5 days) or with cyclophosphamide (CY, 20 mg/kg, i.p.) to female ICR mice. Within PL alone-treated group, WBC and plaque forming cells (PFC) to SRBC were slightly and significantly enhanced when compared with control group. The relative thymus and spleen weights, WBC and PFC numbers were significantly decreased by the treament of CY, whereas those values were markedly increased by the concomitant treatment of CY and PL when compared with CY administration alone. To assess the effects of PL and/or CY on the mitogen response of splenocytes to LPS, mouse splenocytes were stimulated with or without LPS in the presence of various concentration of PL and/or CY in vitro and splenocytes proliferation (SP) was measured by MTT assay. PL alone increased both SP and LPS-stimulated SP. Moreover, SP and LPS-induced SP suppressed by the treament of CY alone were significantly restored by PL-treatment. These activities were higher by PL-M than by PL-W. These results indicated that PL was able to increase humoral immunity and to inhibit immunotoxicity induced by CY.

Chitosan이 마우스의 정상 및 cyclophosphamide로 억제된 일차 체액성 면역반응에 미치는 영향

표명윤(Myoung Yun Pyo),곽영희(Young Hee Kwak) 대한약학회 2002 약학회지 Vol.46 No.2

In order to investigate the effects of chitosan on the normal and cyclophosphamide (CY)-suppressed primary humoral immune response in mice, chitosan was orally administered alone (single dose of 62.5, 250 mg/kg) or with CY (20 mg/kg,I.p.) to female ICR mice on the 2nd day before or after immunization with SRBC-antigen. When chitosan alone was administered before antigenic challenge, splenic IgM plaque forming cells (PFC) and splenic certlularity were slightly increased and serum IgM was not changed when compared with control group. Howeverl chitosan significantly enhanced PFC, serum IgM and splenic cellularity when administered after antigenic challenge. The PFC numbers, serum IgM and splenic cellularity were significantly decreased by the treatment of CY, whereas those values were slightly increased by the concomitant treament of CY and chitosan when compared with CY alone-administration. These results indicate that chitosan is able to increase normal humoral immunity (Hl) and to slightly inhibit the suppressive effects of CY on HI.

In vitro에서 chitosan이 항암제의 세포독성에 미치는 영향

민순홍,표명윤,Min, Soon-Hong,Pyo, Myoung-Yun 환경독성보건학회 2007 환경독성보건학회지 Vol.22 No.3

Chitosan is a depolymerized and partially deacetylated derivative of chitin. We investigated the cytotoxicity of chitosan in cancer cell lines, such as P388, L1210, HCT-15, SK-HepG-1 and mouse splenocytes as a normal cell by MTT assay. To clarify whether chitosan enhances cytotoxicity of anticancer drugs, we also examined the cytotoxicity of combined treatment with chitosan and anticancer drugs, such as cisplatin, mitomycin C, and 5-fluorouracil in cancer cell lines in vitro. Chitosan ($37.5\;{\mu}g/mL,\;75\;{\mu}g/mL,\;112.5\;{\mu}g/mL,\;and\;150\;{\mu}g/mL$) showed concentration-dependent cytotoxicity in the cancer cell lines. In addition, chitosan showed relatively lower cytotoxicity in normal cells than in the cancer cell lines. Particularly, this trend was significant at high doses of chitosan, i.e. $112.5\;{\mu}g/mL,\;and\;150\;{\mu}g/mL$. Thus, these results suggest that chitosan may selectively induce the growth inhibition in cancer cell lines, compared to normal cells. Furthermore. the co-treatment of chitosan and anticancer drugs exhibited an apparant synergistic cytotoxicity in murine lymphoma cell lines, i.e. P388 and L1210 at $37.5\;{\mu}g/mL$ of chitosan rather than at $75\;{\mu}g/mL$ of chitosan, but such phenomenon could not be observed in solid tumor cell lines, i.e. HCT-15 and SK-HepG-1. However, chitosan did'nt reduced the cytotoxicity against normal mouse splenocytes induced by anticancer drugs. Therefore, it is concluded that the combination of chitosan and anticancer drugs might be useful for the cancer chemotherapy.

낙엽송층버섯의 Nitric Oxide 생성저해 물질

장현진,김안근,표명윤,양기숙,Jang, Hyun-Jin,Kim, Ahn-Keun,Pyo, Myoung-Yun,Yang, Ki-Sook 대한약학회 2007 약학회지 Vol.51 No.6

The anti-inflammatory activity of fruit body of Phellinus pini was investigated by activity-guided fractionation. From the screening of each fraction for the inhibitory activity of NO production in lipopolysaccaride (LPS) activated RAW 264.7 cells, methanol extract and its hexane soluble fraction of Phellinus pini exhibited inhibition of NO production compared with LPS control without toxicity. The hexane soluble fraction showed dose-dependent inhibition of NO production. The active hexane fraction was repeatedly chromatographed over silica gel, ergosta-7,24(28)-dien-3-ol(1) and ergosterol peroxide (2) were isolated and identified. Ergosterol derivatives were inhibited NOS activation, $IC_{50}$ of them were $18.9{\pm}3.9{\mu}M$ (1) and $20.4{\pm}4.5{\mu}M$ (2).

Alterations of the immunopathological parameters and immune function of splenocytes in offsprings of mice exposed to bisphenol A

Hae-Ju Kim(김혜주),Myoung-Yun Pyo(표명윤) 환경독성보건학회 2006 한국독성학회 심포지움 및 학술발표회 Vol.- No.-