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한정렬 대한보건협회 2010 건강생활 Vol.- No.69
관동대 제일병원에서 임신부 507명을 조사한 결과 임신부 3명 중 1명인 36.8%가 임신 중 음주 경험이 있었고, 47%는 임신 12주 이후에 임신을 알고서도 음주를 했다고 응답했다. 알코올은 태아의 중추신경계에 영향을 미쳐 학령기 학습장애와 성인기 사회부적응자가 되는 2차 장애를 발생시키므로 임신부는 절대금주 해야 한다.
배양하지 않은 소량의 양수세포에서 Whole Genome Amplification(PEP,DOP)적용과 효율성
김진우,김진미,이봄이,김영미,한정렬,류현미,박소연 한국유전학회 2002 Genes & Genomics Vol.24 No.2
Whole genome amplification (WGA) method to analyze several genes at the same time has become an important issue in prenatal and preimplantation genetic diagnosis. Each 5 amniocytes obtained from 50 pregnant women was preamplified by primer extension preamplification (PEP) and degenerate oligonucleotide primed (DOP)-PCR. From a small aliquot of the each WGA products, we amplified the X-, Y-centromeric sequences, dystrophin gene exon 46 of Duchenne muscular dystrophy (DMD) and survival motor neuron (SMN) gene exon 7 of spinal muscular atrophy (SMA) simultaneously. Compared in 25 male fetuses confirmed with chromosome analysis after two weeks, the detection rates of PEP and DOP methods were 96 and 68 percent in Y-specific amplification, respectively. The amplification rates of X-centromeric region were 100 and 96 percent in PEP and DOP, respectively; 66 and 52 percent of dystrophin gene exon 46; 74 and 46 percent of SMN gene exon 7. Contaminated DNA sequences and false positive for female cells in Y-specific PCR were not detected. This study indicates that the efficiency of PEP was significantly greater than that of DOP in Y-specific and SMN gene (p<0.05).