Photo protective effect of Coumarine on UVB stressed human keratinocyte (HaCaT) cells

Byul-Nim AHN,Jung-Ae KIM,Chang-Suk KONG,Se-Kwon KIM 한국생물공학회 2012 한국생물공학회 학술대회 Vol.2012 No.4

Effect of pH on the Iron Autoxidation Induced DNA Cleavage

Kim, Jong-Moon,Oh, Byul-Nim,Kim, Jin-Heung,Kim, Seog-K. Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.4

Fenton reaction and iron autoxidation have been debated for the major process in ROS mediated DNA cleavage. We compared both processes on iron oxidation, DNA cleavage, and cyclic voltammetric experiment at different pHs. Both oxidation reactions were preferred at basic pH condition, unlike DNA cleavage. This indicates that iron oxidation and the following steps probably occur separately. The ROS generated from autoxidation seems to be superoxide radical since sod exerted the best inhibition on DNA cleavage when $H_2O_2$ was absent. In comparison of cyclic voltammograms of $Fe^{2+}$ in NaCl solution and phosphate buffer, DNA addition to phosphate buffer induced significant change in the redox cycle of iron, indicating that iron may bind DNA as a complex with phosphate. Different pulse voltammogram in the presence of ctDNA suggest that iron ions are recyclable at acidic pH, whereas they may form an electrically stable complex with DNA at high pH condition.

Protective effect of Corydalis heterocarpa on UVB-induced keratinocyte damage

Byul-Nim AHN,Jung-Ae KIM,Chang-Suk KONG,Youngwan SEO,Se-Kwon KIM 한국생물공학회 2011 한국생물공학회 학술대회 Vol.2011 No.4

Mathematical Model for Tsutsugamishi Transmission and Control Strategy of Tsutsugamishi

Byul Nim Kim,Yasuhisa Saito,Yongkuk Kim 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.05

Tsutsugamushi disease, caused by a rickettsial bacteria known as Oriental tsutsugamushi, is one of the most febrile illnesses occurring throughout Korea during fall. O.tsutsugamushi is transmitted by the bite of infected, immature mites of the genus Leptotrombidium. The mites have four-stage life cycles : egg, larva, nymph, and adult. The larva is the only stage that can transmit the disease to humans and other vertebrates, since the other life stages do not feed on the host. During the process of obtaining a blood meal, they may either acquire the infection from the host or transmit the rickettiae to the other rodents or humans. The model can be divided the life cycle of mite into 2 stages : Larva and Adult. And the larva stage can be subdivided according to the phase of activity : questing and feeding. Mites enter either the susceptible questing larvae or infectious questing larvae through birth. When the larva bites an infectious hosts, then the larva moves from the susceptible questing to the infectious feeding class with some probability. On the other hand, hosts also enter the susceptible class through birth. And when an infectious larva bites a susceptible host, then the host enters the infectious class with some probability. In this talk, we introduce our mathematical model for the tsutsugamushi disease, and show the existence and stability condition of the disease-free equilibrium, which is a kind of threshold for the tsutsugamushi disease transmission, and also define the basic reproduction ratio R0. And we discuss about R0 related to all parameters.

Bioactive quinone derivatives from the marine brown alga <i>Sargassum thunbergii</i> induce anti‐adipogenic and pro‐osteoblastogenic activities

Kim, Jung‐,Ae,Karadeniz, Fatih,Ahn, Byul‐,Nim,Kwon, Myeong Sook,Mun, Ok‐,Ju,Bae, Min Joo,Seo, Youngwan,Kim, Mihyang,Lee, Sang‐,Hyeon,Kim, Yuck Yong,Mi‐,Soon, Jang,Kong, C John Wiley Sons, Ltd 2016 Journal of the Science of Food and Agriculture Vol.96 No.3

<P>BACKGROUND: Health problems related to the lack of bone formation are a major problem for ageing populations in the modern world. As a part of the ongoing trend to develop natural substances that attenuate bone loss in osteoporosis, the effects of the edible brown alga Sargassum thunbergii and its active contents on adipogenic differentiation in 3T3-L1 fibroblasts and osteoblast differentiation inMC3T3-E1 pre-osteoblasts were evaluated. RESULTS: Treatment with S. thunbergii significantly reduced lipid accumulation and expression of adipogenic differentiation markers such as peroxisome proliferator-activated receptor gamma , CCAAT/enhancer- binding protein.. and sterol regulatory element binding protein 1c. In addition, S. thunbergii successfully enhanced osteoblast differentiation as indicated by increased alkaline phosphatase activity along raised levels of osteoblastogenesis indicators, namely bone morphogenetic protein-2, osteocalcin and collagen type I. Two compounds, sargaquinoic and sargahydroquinoic acid, were isolated fromactive extract and shown to be active by means of osteogenesis inducement. CONCLUSION: S. thunbergii could be a source for functional food ingredients for improved treatment of osteoporosis and obesity. (C) 2015 Society of Chemical Industry</P>

현대 헤어디자인에 표현된 묶기(tying)기법의 조형성

김별님 ( Byul-nim Kim ),정현진 ( Hyun-jun Jung ),양은진 ( Eun-jin Yang ) 한국미용예술경영학회 2013 미용예술경영연구 Vol.7 No.3

This study examines tying in hair design as they are expressed in the formative arts from the perspective of Post Minimalism to discover its characteristic and formativeness. Expecially. 'tying' form the tree categories of formative arts classified by Maurice Fr´e churent was applied to hair, which is flexible and soft material, and changeability, unpredictability, combinability, and functionality were drawn as the formativeness of tying in modern hair design. Firstly, changeability means that the soft texture of hair is changed from incomplete from hair in th hard, complete fixture through the art tying. Secondly, unpredictability indicates that the soft material of hair can be fixed into a hard structure realizing Avant-garde designs through creative effects. Thirdly, combinability, is achieved when hair is transformed into a new shape by being placed in juxtaposition with image through the tying method to create mystery. Fourthly, hair can be gathered and be tied to accentuate the part where it is tied to draw attention and focus to it. In conclusion, hair as a part of human body can be integrated into the process of creation by adding parameters such as spontaneity to the ever-changing characteristic of hair.

미용서비스 종사자의 미적 노동(Aesthetic Labor) 분석

김별님(Byul Nim Kim),박두현(Doo Hyun Park),양은진(Eun Jin Yang) 한국디자인문화학회 2015 한국디자인문화학회지 Vol.21 No.1

본 연구는 미용서비스 종사자의 시각에서 그들이 종사하는 기업 내에서 미적노동의 과정을 심층면접을 통해 알아보고, 그들의 경험과 변화의 과정을 통해서 미적노동을 이해한 후 미용서비스의 다각적 접근을 조명하고자 수행하였다. 이를 위한 연구문제는 첫째, 미용서비스 종사자들은 미적노동과 관련해 어떤 경험을 해왔는가? 둘째, 미용서비스 종사자들은 미적노동을 통해어떤 변화가 생겼는가? 로 설정하였고, Strauss & Corbin(1998)이 제시한 근거이론 방법을 적용하여 분석하였다. 연구 참여자는 목적표집(Purposive Sampling)에 의해 선정된 프랜차이즈와 토털 뷰티살롱에 근무하는 3년 이상 경력의 뷰티서비스 종사자 9명이었다. 자료수집은 2014년 7월 셋째 주에서 9월 넷째 주에 걸쳐심층면접(In-Depth Interview)을 통해 이루어졌다. 본연구의 결과 개방코딩에서는 67개의 개념과 15개의 하위범주, 6개의 상위범주로 통합되었다. 축코딩에서는 패러다임 모형에 의한 범주분석 결과, 인과적 조건은 채용과 고용과정에서의 미적요구로, 중심현상은 미적노동의 경험으로 나타났다. 맥락적 조건은 기업의 이미지에 따른 외적규율과 교육이었고, 작용/상호작용 전략에 대한 중재조건은 외모관리 필요성의 인지였다. 작용/상호작용 전략은 적극적 외모관리 행동으로 결과는 미적 노동에 대한 내.외적 변화로 도출되었다. 선택코딩에 나타난 핵심범주는 ‘상호적으로 체화되어가는 미용서비스 종사자의 몸과 마음’으로 나타났다. 결론 및 논의에서는 연구의 결과를 토대로 기업의 채용과 고용 그리고 인적관리의 구축에 대한 기여와 미적노동에 대한 미용서비스 종사자와 기업의 참여적인 방향과 후속연구를 위한 학문적, 실무적 시사점과 한계점을 제시하였다. The purpose of this study is to find the experience of “Aesthetic Labor” in the specific work environment from the beauty service employees perspective, and through that process of their experience and change we understand and highlight their aesthetic labor and to contribute presenting various approaches to beauty service studies. The research questions are as followings: First, what experience have the beauty service employees done related to the aesthetic labor? Second, what changes have the beauty service employees experienced through the aesthetic labor? It was analyzed by applying Grounded Theory Approach introduced by Strauss and Corbin(1998). Participants of the study were collected by purposive sampling in 9 beauty service employees who have relevant working experience in franchise or total beauty salon for 3 years or more. Data were collected from third week of July 2014 to fourth week of September 2014 through in-depth interview. As a result of the study, in open coding, the concepts are all 67 in number, the concepts were classified to 15 sub-categories, and integrated to 6 upper-categories. As a result of category analysis for paradigm, in axial coding, causal condition was realized as ‘demand of aesthetic skills in recruitment and selection’, contextual condition was realized as ‘aesthetic training and standard according to the corporate image’, intervening condition that influenced the action/ interaction strategies was ‘recognition of the necessity toward the apparence management’, action/ interaction was ‘active apparence management attitude’, and the result was induced to ‘internal and external change of mind and body about aesthetic labor. Core category in selective coding is ‘reciprocal acquisition of the beauty service employees’ body and mind.’ In conclusion and discussion, based on the result of this study, we contribute to the recruitment and selection of the company and to the establishment of human resource management. Also, we present the theoretical and practical implications and limitations for the follow up studies and the direction for the beauty service employees and corporations to participate on the aesthetic labor.

Emission Detection of Mercuric Ions in Aqueous Media Based-on Dehybridization of DNA Duplexes

Oh, Byul-Nim,Wu, Qiong,Cha, Mi-Sun,Kang, Hee-Kyung,Kim, Jin-Ah,Kim, Ka-Young,Rajkumar, Eswaran,Kim, Jin-Heung Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.9

To quantify the presence of mercuric ions in aqueous solution, double-stranded DNA (dsDNA) of poly(dT) was employed using a light switch compound, $Ru(phen)_2(dppz)^{2+}$ (1) which is reported to intercalate into dsDNA of a right-handed B-form. Addition of mercuric ions induced the dehybridization of poly(dT)${\cdot}$poly(dA) duplexes to form a hairpin structure of poly(dT) at room temperature and the metal-to-ligand charge transfer emission derived from the intercalation of 1 was reduced due to the dehybridization of dsDNA. As the concentration of $Hg^{2+}$ was increased, the emission of 1 progressively decreased. This label-free emission method had a detection limit of 0.2 nM. Other metal ions, such as $K^+$, $Ag^+$, $Ca^{2+}$, $Mg^{2+}$, $Zn^{2+}$, $Mn^{2+}$, $Co^{2+}$, $Ni^{2+}$, $Cu^{2+}$, $Cd^{2+}$, $Cr^{3+}$, $Fe^{3+}$, had no significant effect on reducing emission. This emission method can differentiate matched and mismatched poly(dT) sequences based on the emission intensity of dsDNA.

현대 헤어디자인에 표현된 기하학적 추상 연구

김별님 ( Byul-nim Kim ),양은진 ( Eun-jin Yang ) 한국미용예술경영학회 2013 미용예술경영연구 Vol.7 No.2

New modern hair designs have been created while it has been inseparably related to art styles. Among them, geometric abstraction seeks absolute purity, thus consisting of horizontal and vertical forms with no compositional elements. One of the reasons that geometric forms are well received in these modern times is that they are well arranged and have regular impressions, compared to figurative forms. Geometric hair designs can be defined as works that express abstract forms or inner elements of designers intermingled with their pure formative intention and whose artistry enabling to arouse innovative, novel or visual interest. This study analyzed twentieth-century abstract art styles and artworks for the aesthetic and philosophical investigation of abstraction in art and used such analysis to examine geometric abstract expressions depicted in modern hair designs. As empirical data, it analyzed the works that had won “British Hairdressing Awards.” The study showed that modern geometric hair designs were characterized by clarity, simplicity, repetition and classical tendencies and underlined the formativeness of designs themselves when creating geometric hair design. By investigating modern hair designs through specific art styles, this study could seek for the creative development of design and the expansion of its scope.

Chitooligosaccharides attenuate UVB-induced damages in human dermal fibroblasts.

Ahn, Byul-Nim,Kim, Jung-Ae,Himaya, S W A,Bak, Soon-Sun,Kong, Chang-Suk,Kim, Se-Kwon Springer-Verlag 2012 Naunyn-Schmiedeberg's archives of pharmacology Vol.385 No.1

<P>As a continuation of our research on chitooligosaccharides (COS), this study focused on the protective effect of COS of various molecular weights (1-3, 3-5, and 5-10?kDa) on cellular damage caused by ultraviolet B (UVB)-induced oxidative stress in human dermal fibroblast cells. The results show that the protective effect of COS on UVB-stressed human fibroblasts was dependent on molecular weight. COS suppressed UVB irradiation-induced reactive oxygen species generation and DNA damage, accompanied by the downregulation of matrix metalloproteinase (MMP)-1 and MMP-13. In a comparative analysis, COS (3-5?kDa) exhibited the most potent protective effect on UVB-stressed fibroblasts. The presence of COS (3-5?kDa) attenuated UVB-induced collagenolytic MMP production and collagen degradation. The photoprotective activity of COS (3-5?kDa) was confirmed by transcriptional phosphorylation of mitogen-activated protein kinase-responsive signaling pathways.</P>