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탄소나노튜브 대면적 어셈블리를 통한 고감도-고선택성 과산화수소 센서 개발
이동욱 ( Dongwook Lee ),안희호 ( Heeho Ahn ),서병관 ( Byeong-Gwuan Seo ),이승우 ( Seung-woo Lee ) 한국센서학회 2021 센서학회지 Vol.30 No.4
This study presents a simple approach for the assembly of a free-standing conductive electronic nanofilm of single-walled carbon nanotubes (SWNTs) suitable for enzymatic electrochemical biosensors. A large-scale SWNT electronic film was successfully produced by the dialysis of p-Terphenyl-4,4´´-dithiol (TPDT)-treated SWNTs. Furthermore, Horseradish peroxidase (HRP) was immobilized on the TPDT-SWNT electronic film, and the enzymatic detection of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) was demonstrated without mediators. The detection of H<sub>2</sub>O<sub>2</sub> in the negative potential range (-0.4 V vs. Ag/AgCl) was achieved by direct electron transfer of heme-based enzymes that were immobilized on the TPDT-SWNT electronic film. The SWNT-based biosensor exhibited a wide detection range of H<sub>2</sub>O<sub>2</sub> from 10 μM to 10mM. The HRP-doped SWNT electronic film achieved a high sensitivity of 342 μmA/mM·㎠ and excellent selectivity against a variety of redox-active interfering substances, such as ascorbic acid, uric acid, and acetaminophen.
탄소나노튜브 전자 필름을 이용한 고감도-고선택성 전기화학 글루코스 센서
이승우,이동욱,서병관,Lee, Seung-Woo,Lee, Dongwook,Seo, Byeong-Gwuan 한국공업화학회 2022 공업화학 Vol.33 No.2
This work presents a non-destructive and straightforward approach to assemble a large-scale conductive electronic film made of a pre-treated single-walled carbon nanotube (SWCNT) solution. For effective electron transfer between the immobilized enzyme and SWCNT electronic film, we optimized the pre-treatment step of SWCNT with p-terphenyl-4,4"-dithiol and dithiothreitol. Glucose oxidase (GOx, a model enzyme in this study) was immobilized on the SWCNT electronic film following the positively charged polyelectrolyte layer deposition. The glucose detection was realized through effective electron transfer between the immobilized GOx and SWCNT electronic film at the negative potential value (-0.45 V vs. Ag/AgCl). The SWCNT electronic film-based glucose biosensor exhibited a sensitivity of 98 ㎂/mM·cm<sup>2</sup>. In addition, the SWCNT electronic film biosensor showed the excellent selectivity (less than 4 % change) against a variety of redox-active interfering substances, such as ascorbic acid, uric acid, dopamine, and acetaminophen, by avoiding co-oxidation of the interfering substances at the negative potential value.