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Mariano Michelon,Thais de Matos de Borba,Ruan da Silva Rafael,Carlos André Veiga Burkert,Janaína Fernandes de Medeiros Burkert 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.1
The yeast Phaffia rhodozyma is known for producing carotenogenic pigments, commonly used in aquaculture feed formulation as well as in cosmetic,pharmaceutical, and food industries. Despite the high production of carotenoids from microorganisms by biotechnology, their use has limitation due to the cell wall resistance, which constitutes a barrier to the bioavailability of carotenoids. Therefore, there is a need to improve carotenoids recovering technique from microorganisms for the application of food industries. This study aimed to compare mechanical, chemical, and enzymatic techniques of cell disruption for extracting carotenoids produced by P. rhodozyma NRRL Y-17268. Among the techniques studied, the highest specific concentration of carotenoids (190.35 μg/g) resulted from the combined techniques of frozen biomass maceration using diatomaceous earth and enzymatic lysis at pH of the reaction medium of 4.5 at 55oC, with initial activity of β-1,3 glucanase of 0.6 U/mL for 30 min.
Nathalie Almeida Lopes,Rafael Diaz Remedi,Carolina dos Santos Sa,Carlos Andre´ Veiga Burkert,Janaina Fernandes de Medeiros Burkert 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.3
Since carotenoids are synthesized inside the cell, it is desirable to find an efficient method to extract carotegenic pigments. This study aimed at comparing the effectiveness of different chemical and mechanical techniques to disrupt the cell wall of Sporidiobolus pararoseus and Rhodotorula mucilaginosa yeasts isolated from environmental samples. Among the techniques under study, the ultrasonic bath and the abrasion with glass beads yielded the most promising results for S. pararoseus (84.8 ± 2.3 and 76.9 ± 2.1 lg/g, respectively). The ultrasonic bath yielded the highest specific concentration of carotenoids for R. mucilaginosa (193.5 ± 25.8 lg/g), while the biomass freezing process improved neither the extractability nor the specific concentration of carotenoids. Lyophilization increased the specific concentrations of carotenoids from S. pararoseus and R. mucilaginosa by 20 and 13.7%, respectively, while the freezing process did not significantly affect (p[0.05) the recovery of carotenoids from both yeasts; thus, it may be eliminated from the process.
Cristiane Reinaldo Lisboa,Luciana de Simoni Martinez,Renata Aguirre Trindade,Fátima Aparecida de Almeida Costa,Janaína Fernandes de Medeiros Burkert,Carlos André Veiga Burkert 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6
In this study, a strategy was proposed for making galacto-oligossaccharides (GOS), a high valueadded product, from a byproduct of the dairy industry,cheese whey, using a commercial β-galactosidase from Kluyveromyces lactis (Lactozym® 3000L). The effects of the substrate concentration, temperature, and enzyme dosage were statistically studied and their optimum combinations were determined using response surface methodology. The increase in lactose concentration, temperature, and enzyme concentration favored a transgalactosylation reaction. The maximum values for GOS concentration (119.8 mg/mL)and yield (29.9%) in a 4 h process were obtained in the reaction system, composed of 400 mg/mL of lactose and 10 U/mL of enzyme at 40°C. Under these conditions, the lactose conversion was 68.7%. The maximum value for lactose conversion (87.8%) was observed at the same temperature and enzyme concentration, although the lactose level was 20%.
Moments of the spin structure functions g1p and g1d for 0.05<<sup>Q2</sup><3.0<sup> GeV2</sup>
Prok, Y.,Bosted, P.,Burkert, V.D.,Deur, A.,Dharmawardane, K.V.,Dodge, G.E.,Griffioen, K.A.,Kuhn, S.E.,Minehart, R.,Adams, G.,Amaryan, M.J.,Anghinolfi, M.,Asryan, G.,Audit, G.,Avakian, H.,Bagdasaryan, Elsevier 2009 Physics letters: B Vol.672 No.1
<P><B>Abstract</B></P><P>The spin structure functions <SUB>g1</SUB> for the proton and the deuteron have been measured over a wide kinematic range in <I>x</I> and <SUP>Q2</SUP> using 1.6 and 5.7 GeV longitudinally polarized electrons incident upon polarized NH<SUB>3</SUB> and ND<SUB>3</SUB> targets at Jefferson Lab. Scattered electrons were detected in the CEBAF Large Acceptance Spectrometer, for 0.05<<SUP>Q2</SUP><5<SUP> GeV2</SUP> and W<3 GeV. The first moments of <SUB>g1</SUB> for the proton and deuteron are presented – both have a negative slope at low <SUP>Q2</SUP>, as predicted by the extended Gerasimov–Drell–Hearn sum rule. The first extraction of the generalized forward spin polarizability of the proton γ0p is also reported. This quantity shows strong <SUP>Q2</SUP> dependence at low <SUP>Q2</SUP>. Our analysis of the <SUP>Q2</SUP> evolution of the first moment of <SUB>g1</SUB> shows agreement in leading order with Heavy Baryon Chiral Perturbation Theory. However, a significant discrepancy is observed between the γ0p data and Chiral Perturbation calculations for γ0p, even at the lowest <SUP>Q2</SUP>.</P>
Kim, A.,Avakian, H.,Burkert, V.,Joo, K.,Kim, W.,Adhikari, K.P.,Akbar, Z.,Anefalos Pereira, S.,Badui, R.A.,Battaglieri, M.,Batourine, V.,Bedlinskiy, I.,Biselli, A.S.,Boiarinov, S.,Bosted, P.,Briscoe, W North-Holland Pub. Co 2017 Physics letters. Section B Vol.768 No.-
<P><B>Abstract</B></P> <P>The target and double spin asymmetries of the exclusive pseudoscalar channel e → p → → e p <SUP> π 0 </SUP> were measured for the first time in the deep-inelastic regime using a longitudinally polarized 5.9 GeV electron beam and a longitudinally polarized proton target at Jefferson Lab with the CEBAF Large Acceptance Spectrometer (CLAS). The data were collected over a large kinematic phase space and divided into 110 four-dimensional bins of <SUP> Q 2 </SUP> , <SUB> x B </SUB> , −<I>t</I> and <I>ϕ</I>. Large values of asymmetry moments clearly indicate a substantial contribution to the polarized structure functions from transverse virtual photon amplitudes. The interpretation of experimental data in terms of generalized parton distributions (GPDs) provides the first insight on the chiral-odd GPDs <SUB> H ˜ T </SUB> and <SUB> E T </SUB> , and complement previous measurements of unpolarized structure functions sensitive to the GPDs <SUB> H T </SUB> and <SUB> E ¯ T </SUB> . These data provide a crucial input for parametrizations of essentially unknown chiral-odd GPDs and will strongly influence existing theoretical calculations based on the handbag formalism.</P>
Karine Rech Begnini,Fernanda Nedel,Rafael Guerra Lund,Pedro Henrique de Azambuja Carvalho,Maria Regina Alves Rodrigues,Fatima Tereza Alves Beira,Francisco Augusto Burkert Del-Pino 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.10
Cancer is a leading cause of death and is responsible for one in eight deaths worldwide. The use of herbs as complementary medicine for cancer, especially advanced cancer, has recently increased. The aim of this study was to evaluate in vitro, the antiproliferative effect of Origanum vulgare against human breast adenocarcinoma (MCF-7), and human colon adenocarcinoma (HT-29). The essential oil (EO) was extracted from a bought amount of O. vulgare dried leaves and analyzed in a gas chromatograph interfaced with a mass selective detector. The cytotoxicity test was performed by sulforhodamine B assay. The results show that the EO is composed mostly of 4-terpineol and induces a high cytotoxicity effect in HT-29. In the MCF-7 cell line the EO was less effective. In conclusion, this study showed that O. vulgare main component is 4-terpineol and was effective in inducing cancer cell growth inhibition.