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Namhyeok CHOI,Bumjun PARK,Yun Suk HUH 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Measuring the amounts of free amino acid (FAA) has been steadily needed in areas such as clinical pathology and food hygiene. Current methods to measure the amounts of FAAs is colorimetric assay using Nynhydrin and chromatography, but the Nynhydrin assay has different results depending on the species of FAA, so the results vary depending on the FAA composition of the analyte. For accurate analysis of FAA contents, it is almost the only way to measure and integrate the amount of each FAA by chromatography. However, chromatography is difficult to use in the field because it is time consuming and requires specialized skills. Therefore, there is a need for a technology that can easily and accurately determine the total amount of FAAs in the field. To this end, a carbon paper (CP) electrode modified with L-amino acid oxidase (LAAO) was designed. The LAAO breaks down all kinds of FAA except Proline to produce pyruvic acid, amine, and hydrogen peroxide (HP). HP was selected as the detection target of the electrochemical sensor because pyruvic acid has different species and amine is easily interfered. CP electrode for detecting HP has been functionalized with manganese oxide that can specifically detect HP. Therefore, the developed sensor replaces FAA with HP regardless of species through LAAO attached to the sensor and measures total FAA contents with a signal through HP.