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      • Preparation of aminoclay-polymer nanocomposite hydrogels

        BUI KHAC HOANG VU Gachon University 2017 국내석사

        RANK : 247375

        Aminoclay has been revealed to have potential in many applications. In this research, we used freezing-thawing method for 3 consecutive cycles to prepare poly(vinyl alcohol) (PVA) hydrogels composite with magnesium organophyllosilicate (MgAC) and zinc organophyllosilicate (ZnAC-[Cl2]). These hydrogels were tested by gel fraction, swelling ratio and SEM observation. In general, the presence of aminoclay materials reduced the gel fraction but increased the swelling ratio and porous size of hydrogels. The radial diffusion method was applied for antimicrobial test. Staphylococcus aureus and Escherichia coli were chosen as harmful bacteria. The ZnAC-[Cl2]/PVA hydrogels have stronger antimicrobial activity compared to MgAC/PVA hydrogels. We also prepared MgAC/PVA hydrogels with silver nanowire (AgNW) at different concentration. From initial experiments, silver nanowire enhanced antimicrobial activity but did not affect gel fraction and swelling ratio ability

      • Function of MST1/2 in the Regulation of Breast Cancer Cell Proliferation

        BUI THI CHAU PHUONG 세종대학교 대학원 2018 국내석사

        RANK : 247375

        Mammalian sterile 20-like kinase 1/2 (MST1/2)는 종양 형성에 중요한 역할을 하지만, 유방암 발병 시 MST1/2의 기능은 완전히 밝혀진 것은 아니다. 이 연구에서 MST1/2-siRNA를 이용한 MST1/2 억제를 통해 ER가 존재하는 MCF-7 유방암 세포주의 세포 증식이 효과적으로 억제됨을 확인하였고, ER, cyclin D1, pS2와 같은 유방암세포 성장에 중요한 인자들이 MST2억제로 인해 감소함을 확인할 수 있었다. 게다가 breast invasive carcinoma datasets (from Gene Expression Omnibus) 와 The Cancer Genome Atlas (from Gene set Enrichment Analysis)를 통하여 MST2의 발현과 세포주기 조절에 관련된 gene set의 발현 패턴이 양성적으로 관련이 있음을 확인 할 수 있었고, Kaplan Meier plotter를 통하여 MST2 발현에 따른 유방암 환자의 생존률을 확인한 결과 MST2 발현이 낮은 유방암 환자 그룹이 예후가 더 좋음을 확인할 수 있었다. 이 연구는 MST2와 유방암의 진행과의 관계를 이해하는데 도움을 줄 수 있을 것이라 생각된다. Mammalian sterile 20-like kinase 1/2 (MST1/2), upstream kinase of Hippo pathway, plays important roles in tumorigenesis, yet the function of this factor in breast cancer development is not fully characterized. In this study, I utilized distinct methods of checking cell viability by knock down of MST1/2 to show that the ablation of this kinase dramatically suppresses the proliferation of ER -positive breast cancer cell line (MCF7). I also identify some key proteins that regulate breast cancer cell growth, which are ER, cyclin D1, pS2, are downregulated specifically by MST2 elimination. In addition, breast invasive carcinoma datasets from Gene Expression Omnibus (GEO) (NCBI) and The Cancer Genome Atlas (TCGA), which were subjected to Gene Set Enrichment Analysis (GSEA), suggested that high expression values of STK3 (MST2) were positively coordinated with expression of cell cycle related gene sets. My observations are firmly supported by Kaplan Meier plotter which demonstrates that breast cancer patients with high expression of MST2 have a poorer prognosis than the low-expressed group. This investigation provides a new insight into the relationship between MST2 and the mediation of breast cancer progression. Mammalian sterile 20-like kinase 1/2 (MST1/2)는 종양 형성에 중요한 역할을 하지만, 유방암 발병 시 MST1/2의 기능은 완전히 밝혀진 것은 아니다. 이 연구에서 MST1/2-siRNA를 이용한 MST1/2 억제를 통해 ER가 존재하는 MCF-7 유방암 세포주의 세포 증식이 효과적으로 억제됨을 확인하였고, ER, cyclin D1, pS2와 같은 유방암세포 성장에 중요한 인자들이 MST2억제로 인해 감소함을 확인할 수 있었다. 게다가 breast invasive carcinoma datasets (from Gene Expression Omnibus) 와 The Cancer Genome Atlas (from Gene set Enrichment Analysis)를 통하여 MST2의 발현과 세포주기 조절에 관련된 gene set의 발현 패턴이 양성적으로 관련이 있음을 확인 할 수 있었고, Kaplan Meier plotter를 통하여 MST2 발현에 따른 유방암 환자의 생존률을 확인한 결과 MST2 발현이 낮은 유방암 환자 그룹이 예후가 더 좋음을 확인할 수 있었다. 이 연구는 MST2와 유방암의 진행과의 관계를 이해하는데 도움을 줄 수 있을 것이라 생각된다. Mammalian sterile 20-like kinase 1/2 (MST1/2)는 종양 형성에 중요한 역할을 하지만, 유방암 발병 시 MST1/2의 기능은 완전히 밝혀진 것은 아니다. 이 연구에서 MST1/2-siRNA를 이용한 MST1/2 억제를 통해 ER가 존재하는 MCF-7 유방암 세포주의 세포 증식이 효과적으로 억제됨을 확인하였고, ER, cyclin D1, pS2와 같은 유방암세포 성장에 중요한 인자들이 MST2억제로 인해 감소함을 확인할 수 있었다. 게다가 breast invasive carcinoma datasets (from Gene Expression Omnibus) 와 The Cancer Genome Atlas (from Gene set Enrichment Analysis)를 통하여 MST2의 발현과 세포주기 조절에 관련된 gene set의 발현 패턴이 양성적으로 관련이 있음을 확인 할 수 있었고, Kaplan Meier plotter를 통하여 MST2 발현에 따른 유방암 환자의 생존률을 확인한 결과 MST2 발현이 낮은 유방암 환자 그룹이 예후가 더 좋음을 확인할 수 있었다. 이 연구는 MST2와 유방암의 진행과의 관계를 이해하는데 도움을 줄 수 있을 것이라 생각된다.

      • Effective Tracking Algorithm for Human Following of Mobile Robot with Kinect Sensor

        BUI QUANG TAN 경희대학교 대학원 2017 국내석사

        RANK : 247375

        Abstract EFFECTIVE TRACKING ALGORITHM FOR HUMAN FOLLOWING OF MOBILE ROBOT WITH KINECT SENSOR By Bui Quang Tan Master in Mechanical Engineering Graduate Shool of Kyung Hee University Advised by Ph.D. Soon-Geul Lee Almost half of jobs in the United States are likely to be automated within the next 20 years. That is the prediction from researchers at the University of Oxford in England. They think more and more machines will be given jobs that people traditionally performed. Robots now work in the food industry and build cars. Other robots can be programmed to welcome people as they arrive at a store or hotel. Imagine that you are in an airport and you have to take care your children. You will be very happy if there is a robot it can follow you and help you to carry your luggage. To do the task the robot must have ability to detect, following human and collision a voidance. In this paper, a vacuum cleaning robot platform equipped by Kinect camera sensor to follow a moving human. The system is defined as the Tracking Human Robot System (THRS). This robot is used as a service robot it can track a person quickly and smoothly on a flat floor and maintain a safe distance to that person. There are several sensors they can be used for human tracking. Ultrasonic sensors transmit and receive ultrasonic waves and determine the distance to an object by calculating the time interval between sending the signal and receiving the echo. They are used to track a human and avoid obstacles by developing an ultrasonic-sensor array. However, ultrasonic-sensors cannot distinguish human from object. RGB cameras, which can deliver the three basic color components (red, green, and blue), are frequently used some image processing software for object detection and tracking. For example, OpenCV, Matlab, and Point Cloud Library (PCL) can be used for image processing. However, 3D motion sensors (or depth sensors) are very likely to supersede other sensors in human tracking due to their reliability and depth sensing ability. A 3D motion sensor is a device that can capture motions in 3D. There are two popular commercial 3D motion sensors, Kinect from Microsoft and Xtion PRO Live from Asus. The Kinect sensor has high resolution (1–75mm) and is more adaptable than the Xtion PRO Live. Human-tracking is easy to realize with 3D motion sensors because they usually come with a software development kit (SDK) that processes color, depth, and skeleton data. With these data, it is convenient to create human-tracking applications. Key Words Tracking Human Robot System (THRS), Kinect sensor, vacuum cleaning robot, collision avoidance.

      • A Study on Automatic Ship Berthing System Design with Side Thrusters

        Van Phuoc Bui 부경대학교 2010 국내석사

        RANK : 247373

        This thesis contains some analyses and new results on the problem of automatic ship berthing system design. The main motivation driving this work is to propose a new approach for ship berthing automatically by using only bow and stern thrusters. In this thesis, the ship steering model is considered and simplified to suit the proposed system. Prediction Error Method, one of System identification techniques, is used to estimate the hydrodynamic coefficients of ship motion model. The two-degree-of-freedom servosystem incorporating observer is described. It satisfies two purposes: - Optimal tracking response to step reference by using linear optimal regulator - Robust stability with the uncertainty of model and effect of environmental disturbances In order to evaluate the efficiency of proposed steering model and designed controller, the ship control experiments are performed in model basin. Motion of ship is measured and controlled based on SIMTOOL program through DAQ board. Experimental results show good performances with reduced overshoot and steady state error as well as robustness against environmental disturbances.

      • An Application of?Full AHP to identify the best use of Dong Ngo Closed Landfill Site in Bac Ninh City, Viet Nam

        Thi Hong Hieu BUI 서울시립대학교 2017 국내석사

        RANK : 247372

        Landfills should not be planned in urban areas where populations are densely populated as it is a source of pollutants for the environment. A landfill after reaching its limits will be closed to stop receiving waste, and a matter of concern here is how to reuse the landfill site efficiently. Developers and communities are beginning to consider closed landfills as a potential asset to the community and plan to use the landfill site for different purpose. Many closed landfills have been transformed to new land uses. The new land uses include peon space recreational uses such as parks, wildlife areas, and golf courses and more construction intensive applications such as hard end uos of parking lots and goverment or commercial buldings, woodland for forestry, energy generation, and agricultural land use. The purpose of the study is to find the best land use of a closed landfill site after studying the characterisitics of landfill site by suing Analysis Hierarchy Process (AHP) method. The data used in the study collected from local people surveys and the fifteen experts in three fields such as environment, urban planning, and policy development, the final results were based on their experience in evaluating the weight of criteria and alternatives in the same survey. To achieve the purpose of the study, three main criteria, six sub-criteria, and five alternatives were identified as being relevant to the redevelopment of Dong Ngo landfill site. Hierarchy of of decision levels for Dong Ngo case study has been tested and developed from a real source data and has successfully assessed the best use option as energy generation option for the landfill site under a scenario of decision-making. Keywords: Closed landfill site, redevelopment, after-options, AHP 매립은 환경오염원이기 때문에, 인구가 밀집된 도심지역에서는 피해야 한다. 한계점에 다다른 이후 쓰레기를 더 이상 매립할 수 없는 사용 종료 매립지는 향후 효율적인 이용 문제가 남게 된다. 최근 개발자들과 지역사회는 사용 종료 매립지를 지역사회의 잠재적 자산으로 생각하기 시작하였으며, 다른 용도로 활용할 계획도 수립한다. 이에 수많은 사용 종료 매립지가 새로운 용도의 땅으로 변화하여 왔다. 즉 공원, 야생동물 지역, 골프장, 등의 레크리에이션 목적의 공간으로 활용되거나, 주차장이나 정부청사, 상업 빌딩, 임업을 위한 삼림 지대, 에너지 재생 지역, 농업용지 등으로 재탄생되기도 한다. 본 연구의 목적은 단계적 분석과정(Analytic Hierarchy Process, AHP)을 사용하여, 매립지의 특성을 연구한 다음 사용 종료 매립지를 가장 잘 활용할 수 있는 방법을 모색하는 것이다. 본 연구에서는 현지인 조사 및 환경, 도시+J47계획, 정책개발 분야의 15명의 전문가로부터 수집한 데이터를 사용했고, 동일 조사에서 기준의 가중치 및 대안 평가에서 그들의 경험에 기반하여 최종 결과를 내놓았다. 본 연구의 목적상, 동엔고(Dong Ngo)매립지의 재개발과 관련하여 나온 세 개의 1차적 기준, 6개의 2차적 기준 및 5개의 대안을 사용하였다. 동엔고 사례 연구와 관련한 결정 수준의 계층을 실질원 데이터에서 개발, 시험하였고, 의사결정 시나리오 하에서, 매립지에 대한 에너지 생산 옵션으로서, 최선의 활용 옵션을 평가하는 데에 성공했다. 주요 용어: 사용종료 매립지, 재개발, 사용 후 옵션, AHP

      • Development of Fluorescence- base Rapid Diagnostic System for Infectious Disease : 감염병의 현장진단용 형광체기반진단 시스템개발

        BUI THI CUC 원광대학교 일반대학원 2017 국내석사

        RANK : 247359

        A new method is emergence for the detection of pandemic influenza caused by H5 subtype. The major advantages of quantum dots (QDs) such as high signal intensity and photostability are integrated with the immunochromatographic assay (ICA) leading to high sensitivity, good specificity for rapid fluorescent diagnostic kit of Avian influenza virus (AIV) infection. In this study, in order to improve the sensitivity of rapid diagnostic system, we have designed the application of advantages of quantum dots (QDs) to rapid diagnostic system, using a covalent QDs conjugation based on crosslinking reactions between amine and carboxylic acid groups. After conjugation of QD to latex, the complex of latex beads was conjugated with antibody (Latex- QDs- Ab) making the final bioconjugate complex. The bioconjugate complex was applied on nitrocellulose strips coated with monoclonal antibodies (mAbs) against hemagglutinin (HA) of the H5 subtype. By examination some parameters (amount of QDs, conjugation buffer, standard of dry conjugate on membrane and lysis buffer), we established rapid H5 subtype-specific fluorescent immunochromatographic test (QD-FICT) and it was compared with commercial diagnostic kit. As a result, we found that the optimal conditions of QD-FICT (Latex: QD molar ratio is 1: 500), MES conjugation buffer as conjugation buffer to improve the sensitivity. In addition, a solution of 4% sucrose and, 1% Triton X100 on the surface of strips was helpful for migration of conjugate. Besides, lysis buffer including 0.5% sodium deoxy cholate reduced the nonspecific interaction of antibody on the strip. The limit of detection (LOD) of our rapid kit was 5 HAU/mL of H5N3, which is 64 times more efficient than commercial rapid diagnostic kit. The specificity of QDs conjugate was confirmed by testing with other subtypes (H1N1, H7N1, and H7N7). These results suggest that H5 subtype-specific FICT using QD was developed successfully and will be useful to prevent and control Influenza A H5 subtype virus infection in the field 인플루엔자 A 바이러스 중 H5N1 아형 바이러스는 2003년 이래 동남아시아를 비롯하여 중동까지 확산되고 있고 치사율 60%로, 현재까지 700여명의 사망을 초래하고 있으며, 동물에서 사람에서 전파될 뿐 아니라 사람과 사람간 발생 가능성도 있다. 따라서 발생시 현장에서 환자를 바로 격리하고 급성 폐렴에 대해 바로 치료를 시작해야 사망률을 감소시킬 수 있으나, 현장검사방법은 RDT와 같은 진단키트로서 사람의 H5 아형을 바로 진단할 수 없는 단점이 있어 아형 특이 진단 시스템을 개발해야 한다. 본 연구에서는 quantum dots (QDs)를 H5 아형 특이 항체에 축합하여 이를 신속형광면역진단시스템 (QD-FICT)을 개발하였다. 신속형광면역진단시스템을 최적화 및 안정화 시키는 조건을 확립하여 축합 버퍼로는 (Latex: QD molar ratio is 1: 500), MES buffer, 4% sucrose 및 0.1% Triton X-100의 형광체 건조 효과, 바이러스 용해버퍼에 0.5% sodium deoxy cholate가 가장 QD-FICT에 적합함을 확인하였다. 이를 통해 H5-특이 QD-FICT는 상용화된 진단키트보다, 바이러스 검출율이 64배 증가되었고, H5 아형 특이성을 보유하고 있음을 확인하였다. 결론적으로 본 연구는 QD 및 H5-특이항체를 이용하여 신속형광면역진단시스템을 성공적으로 개발하였고, 이는 향후 현장에서 H5 아형 바이러스의 특이 진단에 유용하게 활용될 것으로 사료된다.

      • 온칩 커뮤니케이션용 등화기능을 갖는 고속 직병렬 데이터 전송회로 설계

        BUI CHINH HIEN 충북대학교 대학원 2013 국내석사

        RANK : 247359

        This thesis presents a wave-pipelined (WP) serializer - deserializer (SerDes) with asynchronous protocols and equalizers for high speed serial on-chip communications. The proposed equalization scheme consists of a 4-tap pre-emphasis or feedforward equalizer at the transmitter side and a 2-tap decision feed-back equalizer at the receiver side. A differential global on-chip interconnect pair is modeled and analyzed in a 3-D EM field solver to estimate the interconnect loss. Parameters of the target differential interconnect pair are 10-mm long, 0.5-μm thick, and 0.5-μm spacing. It is implemented on metal 5 layer in 130 nm CMOS process. A program built in Matlab is used to calculate equalizer parameters. Simulation results show that the proposed scheme can compensate for the intersymbol interference and the loss of the interconnects mentioned above at data rate up to 4Gb/s which is enabled by the proposed WP SerDes. Power consumption of the proposed WP SerDes with equalizers is 10 mW. Energy of the proposed equalizers is 2.45 pJ/bit. Using asynchronous protocols, the proposed WP SerDes reduces power consumption and circuit complexity compared with the conventional synchronous SerDes. The equalizers make the transmitted signals realizable after travelling through the lossy on-chip interconnects. The proposed WP SerDes can be applied in SoC or mobile applications that require high-speed and low-power on-chip communications.

      • Total phenolic compounds and flavonoids in the parts of artichoke (Cynara scolymus L.) in Vietnam : 베트남의 엉겅퀴(Cynara scolymus L.)에 함유된 페놀 화합물과 flavonoids

        BUI THI THU HA Graduate School Daegu Haany University 2008 국내석사

        RANK : 247359

        Artichoke extracts are widely used alone or in association with other herbs for embittering alcoholic and soft drinks and to prepare herbal teas or herbal medicinal products in Vietnam. The objective of this paper was a screening of flavonoids and total phenolic compounds content in the parts of artichoke (Cynara scolymus L.) as flowers, leaves, roots, trunks, stumps. The total phenolic compounds and flavonoids in the parts of artichoke were extracted among 3 extraction methods as methanol extraction (EM1), mixing methanol and water method (EM2) and water extraction method (EM3. Total phenolic compounds and flavonoids were determined by UV/VIS, HPLC techniques. The apigenin 7-O-glucosides, cynarin, narirutin, gallic acid, caffeic acid were found as the main flavonoids constituents in all parts of artichoke. It showed that value of total phenolic compounds and flavonoids by EM3 were higher than that of total phenolic compounds and flavonoids by EM1 and EM2. Furthermore, the results of this study revealed that total phenolic compounds and flavonoids, obtained by these convenient extraction methods, may show the quick efficacy of artichoke in all respects of their quality and quantity. 베트남에서 엉겅퀴 추출물은 허브차나 약제품을 만들거나 알콜 음료나 청량음료에 쓴맛을 가미하기 위해 사용된다. 본 연구에서는 엉겅퀴(Cynara scolymus L.)의 꽃, 잎, 뿌리, 줄기, 그루터기 부분에 함유되어 있는 페놀화합물과 flavonoids의 추출방법에 대해 비교 검토하였다. 추출방법은 methanol 추출방법(EM1), methanol과 물 혼합 추출방법(EM2) 그리고 물 추출방법(EM3)을 사용 하였으며, 페놀화합물과 flavonoids의 분석은 UV와 HPLC을 사용하였다. 엉겅퀴에서 꽃, 잎, 뿌리, 줄기, 그루터기에서 주요flavonoids로 apigenin 7-O-glucosides, cynarin, narirutin, gallic acid, caffeic acid가 학인되었다. 페놀화합물과 flavonoids의 추출은 EM1과EM2보다 EM3가 가장 효율적이었다. 따라서 본 연구에서는 물 추출법을 사용 함으로서 엉겅퀴 주요 성분인 페놀화합물과 flavonoids가 효율적으로 추출되는 것으로 나타났다.

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