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RISS 인기검색어
- 검색키워드
- 저자 : Boping Zhang (검색결과 5 건)
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The Study of Visual Measurement Based on GPS Navigation
Boping Zhang 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.4
GPS has its weaknesses in reality. This paper introduces vision measurement for the GPS system. Through feature detection, stereo matching, feature tracking and motion estimation to 3D drawings, GPS is able to get an accurate location and an estimation of direction with less error. Experiment has proved that vision measurement can be applied into navigation in the steep and rugged terrain. The improved GPS system is more accurate and can be put into practice in vaster areas.
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Copper Acetate Etching of Colloidal CdSe Nanocrystals
Boping Yang,Huichao Zhang,Jiayu Zhang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.2
Colloidal CdSe nanocrystals (NCs) were etched after Se/TBP and Zinc stearate/ODE were injected into the mixture of as-prepared CdSe NCs and Copper (II) acetate in ODE solvent. Spectroscopic and structural investigations demonstrate the etching process. Along with the etching time, both the absorption and photoluminescence (PL) spectra of etched NCs showed blue-shift while the transmission electron microscopy (TEM) images indicated that the size of the NCs became from 5.6 nm to 2.6 nm. X-ray diffraction (XRD) patterns suggested that no other clusters or core/shell NCs were formed in the etching process and inductively coupled plasma (ICP) data demonstrated that only selenium and cadmium comprised the etched NCs. Electronic paramagnetic resonance (EPR) spectra indicated the deoxidization of Cu2+ ions and suggested the etching mechanism through cation exchange process.
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Chenchen Shen,Dan Tang,Yiping Zhang,Lv Wu,Yaqi Luo,Boping Tang,Zhengfei Wang 한국유전학회 2021 Genes & Genomics Vol.43 No.5
Background Olfaction plays a central role in mating, spawning, obtaining food and escaping predators, which is essential for survival and reproduction of animals. The nature of the olfactory perception in crabs, which is a major group of crustaceans, has remained elusive. Objective This project aims to explore the molecular mechanism of olfaction in crabs and further improve our understanding of olfactory perception in crustaceans. Methods The olfactory receptors and ingestion-related gene expression in Eriocheir japonica sinensis were studied by transcriptomic techniques. The de novo assembly, annotation and functional evaluation were performed with bioinformatics tools. Results A series of chemosensory receptors associated with olfaction were identifed including 33 EsIRs, 24 EsIGluRs, 58 EsVIGluRs, 1 EsOR and 1 EsGC-D. We found IRs were key odorant receptors demonstrating a specifc species evolutionary trend in crustaceans. Furthermore, we identifed ORs in E. j. sinensis and Litopenaeus vannamei. The incomplete EsOR and LvOR1 structures implied that ORs exist in crustaceans, and may have been degenerated or even lost in the olfactory evolutionary process. In addition, comparative transcriptome analysises demonstrated two possible olfactory transduction pathways of E. j. sinensis: the cGMP-mediated olfactory pathway related to vegetable odor molecules and the cAMP-mediated olfactory pathway related to meat odor molecules. The above results were consistent with its omnivorous ingestion of E. j. sinensis. Conclusions Our study revealed the unique olfactory molecular mechanism of omnivorous crabs and provided valuable information for further functional research on the chemoreception mechanisms in crustaceans.
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Molecular cloning, subcellular localization, and expression of BsWRKY51 gene from Bletilla striata
Wang Shuangshuang,Zheng Yuxia,Dou Quanli,Zhang Zhengling,Zeng Boping,Li Ying,Qian Yongchun,Ma Li 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.2
The WRKY transcription factor family plays a key role in plant growth and development, hormone signaling, and resistance to environmental stress. In this study, we investigated the gene sequence, subcellular localization, and response pattern of a member of the WRKY transcription factor family to reveal its protein structure and involvement in the resistance signaling pathway.The BsWRKY51 gene was cloned by RT-PCR, and the structural characteristics of its encoded protein WRKY51 were analyzed by bioinformatics. The vector was next transiently transformed into tobacco to analyze the subcellular locali- zation, and real-time fluorescence quantitative PCR was performed to analyze the changes in the expression pattern of BsWRKY51. The BsWRKY51 gene has a coding sequence (CDS) length of 987 bp.The respective unstable hydrophilic protein BsWRKY51 is localized in the nucleus. It most closely related to the WRKY protein of Dendrobium catenatum in the Orchidaceae family. Fluorescence quantitative PCR results showed that the BsWRKY51 expression in the leaves was significantly higher than that in the roots, stems, and pseudobulbs of Bletilla striata seedlings. Under the conditions of salt and drought stress, the BsWRKY51 expression gradual increased and then a slightly decreased, and under salicylic acid (SA) treatment, the expression of BsWRKY51 showed an overall decreasing trend.The BsWRKY51 gene of Bletilla striata may play an important regulatory role in its salt and drought stress responses. Our present findings provide the foundation for elucidating the mechanisms of salt and drought tolerance in Bletilla striata and for breeding new varieties.
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( Wen Fei Xue ),( Jing Fu Peng ),( Xiao Li Yu ),( Shu Lin Zhang ),( Boping Zhou ),( Dan Qing Jiang ),( Jian Bo Chen ),( Bing Bing Ding ),( Bin Zhu ),( Yao Li ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1
The widespread occurrence of drug-resistant Mycobacterium tuberculosis places importance on the detection of TB (tuberculosis) drug susceptibility. Conventional drug susceptibility testing (DST) is a lengthy process. We developed a rapid enzymatic color-reaction-based biochip assay. The process included asymmetric multiplex PCR/templex PCR, biochip hybridization, and an enzymatic color reaction, with specific software for data operating. Templex PCR (tem- PCR) was applied to avoid interference between different primers in conventional multiplex- PCR. We applied this assay to 276 clinical specimens (including 27 sputum, 4 alveolar lavage fluid, 2 pleural effusion, and 243 culture isolate specimens; 40 of the 276 were non-tuberculosis mycobacteria specimens and 236 were M. tuberculosis specimens). The testing process took 4.5 h. A sensitivity of 50 copies per PCR was achieved, while the sensitivity was 500 copies per PCR when tem-PCR was used. Allele sequences could be detected in mixed samples at aproportion of 10%. Detection results showed a concordance rate of 97.46% (230/236) in rifampicin resistance detection (sensitivity 95.40%, specificity 98.66%) and 96.19% (227/236) in isoniazid (sensitivity 93.59%, specificity 97.47%) detection with those of DST assay. Concordance rates of testing results for sputum, alveolar lavage fluid, and pleural effusion specimens were 100%. The assay provides a potential choice for TB diagnosis and treatment.
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