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- 저자 : Binlei Liu (검색결과 2 건)
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Han Hu,Changsheng Jiang,Binzhou Zhan,Nan Guo,Zhonghua Li,Xiaozhen Guo,Yang Wang,Binlei Liu,Qigai He 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.5
Background: Antimicrobial peptides (AMPs) have been identified as promising compounds for consideration as novel antimicrobial agents. Objectives: This study analyzed the efficacy of cecropin B against Haemophilus parasuis isolates through scanning electron microscopy (SEM) and atomic force microscopy (AFM) experiments. Results: Cecropin B exhibited broad inhibition activity against 15 standard Haemophilus parasuis (HPS) strains and 5 of the clinical isolates had minimum inhibition concentrations (MICs) ranging from 2 to 16 μg/mL. Microelectrophoresis and hexadecane adsorption assays indicated that the more hydrophobic and the higher the isoelectric point (IEP) of the strain, the more sensitive it was to cecropin B. Through SEM, multiple blisters of various shapes and dents on the cell surface were observed. Protrusions and leakage were detected by AFM. Conclusions: Based on the results, cecropin B could inhibit HPS via a pore-forming mechanism by interacting with the cytoplasmic membrane of bacteria. Moreover, as cecropin B concentration increased, the bacteria membrane was more seriously damaged. Thus, cecropin B could be developed as an effective anti-HPS agent for use in clinical applications.
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Bin Lei,Wengui Li,Huajian Liu,Zhuo Tang,Vivian W. Y. Tam 한국콘크리트학회 2020 International Journal of Concrete Structures and M Vol.14 No.5
To better understand the synergistic effects of combined fibers on mechanical properties and durability of recycled aggregate concrete (RAC), different types of fibers with various lengths and mass ratios were adopted in this study. Experimental investigations were conducted to study the 28-day compressive strength and strength loss after exposed to salt-solution freeze–thaw cycles and the coupled action of mechanical loading and salt-solution freeze–thaw cycles. The microstructure was also characterized to evaluate the mechanism of this synergistic effect. To determine the effectiveness of the combined fibers on improving the mechanical properties and durability of RAC, the synergistic coefficient was proposed and applied for various combinations of fibers. The results indicate that the incorporation of fibers slightly decreased the 28-day compressive strength of RAC, but combining different sizes and types of fibers can mitigate this negative effect. Moreover, the incorporation of fibers greatly improves the freeze–thaw resistance of RAC. The combining different fibers exhibited a synergistic effect on the enhancement in properties of RAC, which could not be predicted with only one simplistic rule of fibre mixtures. In addition, microstructural characterization shows that the bonding strength of the interfacial transition zone (ITZ) between the fiber and cement matrix is mainly determined by the chemical bonding force which is due to the hydration reaction between fiber surface and cement matrix.
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