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The Degradation of Chitin with Food Grade Papain
(Beom Ku Han,(Tak You,(Jong Kook Moon,(Sae Bom Kim,(Do Hyun Jo 한국응용생명화학회 2000 Journal of Applied Biological Chemistry (J. Appl. Vol.43 No.4
We investigated the production of chitin oligosaccharides using food grade papain. A solution of commercial food grade papain (FGP) was dialyzed for 12 h before measuring its chitinolytic activity. The effects of enzyme concentration, reaction temperature
CALCULATION OF THE STRUCTURAL STIFFNESS OF RUN-FLAT AND REGULAR TIRES BY CONSIDERING STRAIN ENERGY
Jong-Jin Bae,Youngbin You,Jong Beom Suh,Namcheol Kang 한국자동차공학회 2019 International journal of automotive technology Vol.20 No.5
The vertical stiffness of a tire is defined by the ratio of the vertical force to the deflection; it can be expressed as the combination of the structural stiffness and air stiffness. However, the calculation of the structural stiffness alone is challengeable, and therefore this paper presents a new methodology for extracting the structural stiffness from the strain energy of the regular and run-flat tires. In order to verify our proposed method, the applied force-deflection relationship was compared with the calculated force-deflection relationship using the proposed energy method at zero air pressure. Further, the structural stiffness of the inflated regular and run-flat tires was calculated. In addition, we obtained the structural stiffness of the tire components to investigate the effect of each component on the overall structural stiffness of both tires. Finally, we analyzed the vertical and structural stiffness of the run-flat tire when the inflation pressure and the elastic modulus of the components were varied.
Allelic analysis of low molecular weight glutenin subunits using 2-DGE in Korean wheat cultivars
Lee, Jong-Yeol,Jang, You-Ran,Beom, Hye-Rang,Altenbach, Susan B.,Lim, Sun-Hyung,Lee, Choung Keun Japanese Society of Breeding 2017 Breeding science Vol.67 No.4
<P>Two-dimensional gel electrophoresis (2-DGE) was used as a complement to SDS-PAGE to determine the allelic compositions of LMW-GS in 32 Korean wheat cultivars. Protein patterns generated by 2-DGE from each cultivar were compared to patterns from standard wheat cultivars for each allele. At the <I>Glu-A3</I> locus, thirteen <I>c</I>, twelve <I>d</I>, three <I>e</I> (null), two g and two new alleles were identified. At the <I>Glu-B3</I> locus, one <I>b</I>, nineteen <I>d</I>, four <I>h</I>, one <I>i</I> and five <I>ad</I> alleles were identified. At the <I>Glu-D3</I> locus, twenty-three <I>a</I>, four <I>b</I>, four <I>c</I> and one <I>l</I> alleles were identified. When compared to results obtained previously using SDS-PAGE, there were discrepancies in the allelic designations of 10 of 32 cultivars (31%). While SDS-PAGE is a rapid and relatively simple method for assessing LMW-GS composition, the similar mobilities of the proteins makes it difficult to discriminate certain alleles. 2-DGE is a more complicated technique, but provides a more accurate picture of the complement of the LMW-GS in a given cultivar. In addition to providing essential information for wheat breeders, the 2-DGE reference maps generated in this study will make it possible to study the contributions of individual LMW-GS to flour quality.</P>