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Patil Suvarna,Bashasab Fakrudin,Vijaykumar Vijaykumar,Basavanagoud Basavanagoud,Kuruvinashetti Mahaling S,Patil Basavaraj V Korean Society of Applied Entomology 2006 Journal of Asia-Pacific Entomology Vol.9 No.3
The genetic relatedness among Helicoverpa armigera ($H\"{u}bner$) occurring on different host plants prevailing in South India was studied using PCR-RAPD. Genomic DNA was isolated individually from five larvae collected from each of 10 different host plants (except in okra). PCR-RAPD analysis was carried out using a set of 20 random primers which had produced repeatable banding patterns from a original set of 60 primers. A set of 155 amplicon levels were available for analysis, of which 154 were polymorphic. An average of 7.75 bands per primer was recorded. Similarity coefficients based on the frequency of band sharing among host strains varied from 0.25 in cotton and sunflower to 0.72 in groundnut. Clustering analysis on the basis of the PCR-RAPD-generating band sharing indicated that most of the individuals occurring on niger, safflower, green gram, abutilon and lagasca clustered together, indicating greater genetic similarity among themselves, than those occurring on other crops. Furthermore, the pattern of genetic variation in the individuals collected from niger, safflower, green gram, groundnut, abutilon and lagasca was seem to be largely host-dependent.
Development and evaluation of psyllium seed husk polysaccharide based wound dressing films
Patil, Basavaraj S.,Mastiholimath, Vinayak S.,Kulkarni, Anandrao R. 경희한의학연구센터 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.2
In the present study, wound dressing films were fabricated using Seed Husk of Psyllium (SHP) complexed with povidone iodine and were evaluated for various physicochemical properties as well as wound healing activity in albino rats. The required film properties for the successful wound dressings, such as film elongation (elasticity), tensile strength, water uptake and water vapor transmission rates (WVTR) were examined. Results indicated that SHP films showed good elasticity (80.27-116.24%) and acceptable tensile strengths (8.33-22.13 N/$^mm2$). SHP films were evaluated for water absorption pattern as well as permeation to water vapours and results showed that films were able to swell in water upto 167 to 191% w/w and WVTR were in the range between $8.23{\times}10^{-3}$ to $1.12{\times}10^{-4}g.cm^2/day$. Selected formulation of SHP films with optimum characteristics were loaded with Povidone iodine (PI) solution by soaking method and were evaluated for antimicrobial and wound healing activity on excision wound model. Results indicated that PI loaded films showed significant antibacterial activity against both gram positive and gram negative selected bacteria. PI loaded SHP films showed comparatively better wound healing property to control group with faster epithelialization and greater rates of wound contraction.
Development and evaluation of psyllium seed husk polysaccharide based wound dressing films
Basavaraj S. Patil,Vinayak S. Mastiholimath,Anandrao R. Kulkarni 경희대학교 융합한의과학연구소 2011 Oriental Pharmacy and Experimental Medicine Vol.11 No.2
In the present study, wound dressing films were fabricated using Seed Husk of Psyllium (SHP) complexed with povidone iodine and were evaluated for various physicochemical properties as well as wound healing activity in albino rats. The required film properties for the successful wound dressings, such as film elongation (elasticity), tensile strength, water uptake and water vapor transmission rates (WVTR) were examined. Results indicated that SHP films showed good elasticity (80.27–116.24%) and acceptable tensile strengths (8.33–22.13 N/mm2). SHP films were evaluated for water absorption pattern as well as permeation to water vapours and results showed that films were able to swell in water upto 167 to 191% w/w and WVTR were in the range between 8.23×10−3 to 1.12×10−4 g.cm2/day. Selected formulation of SHP films with optimum characteristics were loaded with Povidone iodine (PI) solution by soaking method and were evaluated for antimicrobial and wound healing activity on excision wound model. Results indicated that PI loaded films showed significant antibacterial activity against both gram positive and gram negative selected bacteria. PI loaded SHP films showed comparatively better wound healing property to control group with faster epithelialization and greater rates of wound contraction.
Suvarna Patil,Fakrudin Bashasab,Vijaykumar,Basavanagoud,Mahaling S Kuruvinashetti,Basavaraj V Patil 한국응용곤충학회 2006 Journal of Asia-Pacific Entomology Vol.9 No.3
The genetic relatedness among Helicoverpa armigera (Hübner) occurring on different host plants prevailing in South India was studied using PCR- RAPD. Genomic DNA was isolated individually from five larvae collected from each of 10 different host plants (except in okra). PCR-RAPD analysis was carried out using a set of 20 random primers which had produced repeatable banding patterns from a original set of 60 primers. A set of 155 amplicon levels were available for analysis, of which 154 were polymorphic. An average of 7.75 bands per primer was recorded. Similarity coefficients based on the frequency of band sharing among host strains varied from 0.25 in cotton and sunflower to 0.72 in groundnut. Clustering analysis on the basis of the PCR-RAPD-generating band sharing indicated that most of the individuals occurring on niger, safflower, green gram, abutilon and lagasca clustered together, indicating greater genetic similarity among themselves, than those occurring on other crops. Furthermore, the pattern of genetic variation in the individuals collected from niger, safflower, green gram, groundnut, abutilon and lagasca was seem to be largely host-dependent.
Recent advancements in lipid–mRNA nanoparticles as a treatment option for cancer immunotherapy
Karmacharya Prajeena,Patil Basavaraj Rudragouda,김종오 한국약제학회 2022 Journal of Pharmaceutical Investigation Vol.52 No.4
Background Cancer remains a serious health concern worldwide, and different approaches are being developed for its treatment. The strategy to use the immune system as an approach for treating cancer has recently gained momentum. Messenger RNA (mRNA) has been assessed as an up-and-coming resource for the evolution of advanced cancer immunotherapies over the past decades. However, degradation in extracellular compartments and during endosomal escape remain obstacles for efficient mRNA delivery and limit the therapeutic applications of this approach. Area covered Lipid-based nanocarriers are gaining significant attention as non-viral mRNA vectors. Various lipid-based nanocarrier types have been developed to enhance the stability of mRNA molecules, facilitate their transfection, and ensure delivery to an intracellular compartment suitable for further processing. This review discusses the development of novel mRNA delivery systems using lipids for effective cancer immunotherapy. Expert opinion mRNAs are superior to other biomolecules for developing therapeutic drugs and vaccines with multiple medical applications that are currently being explored by researchers in various biomedical fields. Lipid-based mRNA nanoparticles can improve the potency of the mRNA by enhancing its stability, enabling its cellular uptake, and facilitating its endosomal escape. Targetability of these therapeutics can be increased by conjugating their surface with the desired ligands or targeting agents. Lipid–mRNA nanoparticles are increasingly being incorporated in cancer immunotherapy applications, including vaccines, monoclonal antibodies, and chimeric antigen receptor T-cell treatment, and several such nanoparticles are being assessed in clinical trials. Further research that assesses key variables for transfection efficiency of lipid–mRNA nanoparticles will expedite the development of improved therapeutics.
Bashasab Fakrudin,Vijaykumar Vijaykumar,Kambalapally Krishnareddy Bayyareddy,Kuruvinashetti Mahaling Shrishailappa,Patil Basavaraj Veeranagouda Korean Society of Applied Entomology 2007 Journal of Asia-Pacific Entomology Vol.10 No.1
Morphometric characterization of twelve geographic populations of cotton bollworm, Helicoverpa armigera occurring in south Indian cotton ecosystems was done at larval, pupal and adult stages over three cropping seasons. Traits such as length and weight of larvae, pupa and length and width of the wing, length of fore-, mid- and hind femur, male reproductive organ-length of genital capsule, valves, and ejaculatory duct, female reproductive organ-length of appendix bursae and ductus bursae at adult stage were measured across three years. Populations significantly differed for most of the traits studied. It was evident that populations from northern parts recorded higher phenotypic attributes compared to those from southern parts of south Indian cotton ecosystem. Besides larval, pupal and adult external phenotypic traits, attributes of male reproductive organ viz., length of genital capsule, valves, and ejaculatory duct and female reproductive organ viz., length of appendix bursae and ductus bursae differed significantly among populations. Information on population structure and differences based on morphometry would be useful in better understanding of population dynamics and management of this pest in cotton and other crops in south India.
Fakrudin Bashasab,Vijaykumar,Krishnareddy Bayyareddy Kambalapally,Mahaling Shrishailappa Kuruvinashetti,Basavaraj Veeranagouda Patil 한국응용곤충학회 2007 Journal of Asia-Pacific Entomology Vol.10 No.1
Morphometric characterization of twelve geographic populations of cotton bollworm, Helicoverpa armigera occurring in south Indian cotton ecosystems was done at larval, pupal and adult stages over three cropping seasons. Traits such as length and weight of larvae, pupa and length and width of the wing, length of fore-, mid- and hind femur, male reproductive organ-length of genital capsule, valves, and ejaculatory duct, female reproductive organ-length of appendix bursae and ductus bursae at adult stage were measured across three years. Populations significantly differed for most of the traits studied. It was evident that populations from northern parts recorded higher phenotypic attributes compared to those from southern parts of south Indian cotton ecosystem. Besides larval, pupal and adult external phenotypic traits, attributes of male reproductive organ viz., length of genital capsule, valves, and ejaculatory duct and female reproductive organ viz., length of appendix bursae and ductus bursae differed significantly among populations. Information on population structure and differences based on morphometry would be useful in better understanding of population dynamics and management of this pest in cotton and other crops in south India.