Differential Protein Expression Profiling in Pleurotus ferulae Mycelium Caused by Asafoetida Extracts Using a Proteomics Approach

Yujia Bai,Wenshu Huang,Yongxia Tao,Zuoshan Feng 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.1

The precise mechanism by which asafoetida extractinfluenced the growth of Pleurotus ferulae mycelium wasinvestigated using a comparative proteomic analysis approach. Two types of asafoetida extracts were added to the medium of P. ferulae, and the effects of the extract on microstructure of P. ferulae mycelium were observed. Compared to the control group,the two asafoetida extracts played a significant role in promotingthe growth of P. ferulae. Two-dimensional electrophoresis andLC-MS/MS were performed to examine changes in the expressionof proteins in the mycelium of P. ferulae. A total of eightdifferentially expressed proteins were identified, including a heatshock protein, a flavin-containing monooxygenase, and a NADPH:quinone oxidoreductase type IV, as well as hypothetical andunknown proteins. Expression change of these proteins confirmedthat asafoetida extracts significantly affected P. ferulae growth,metabolism, and secondary product metabolism. These resultsprovide new insights into biochemical mechanisms underlying therelationship between P. ferulae and asafoetida.

Phenylpropanoid metabolism enzyme activities and gene expression in postharvest melons inoculated with Alternaria alternata

Bai Yujia,Feng Zuoshan,Paerhati Maerhaba,Wang Jin 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.6

This study explored the mechanism of melon resistance to Alternaria alternata (A. alternata) infection in Jiashi and 86-1 melons. Melons were inoculated with A.alternata and the change in lesion diameter was measured. The changes in cinnamic acid-4-hydroxylase (C4H), phenylalanine ammonia lyase (PAL), and 4-coumaric acid coenzyme A ligase (4CL) activity and gene expression were studied in the pericarp tissues of Jiashi and 86-1 melons. The lesion diameter was smaller in Jiashi melon than in 86-1 melon, and the pericarp lesions were smaller than pulp lesions, indicating that Jiashi melon can resist A. alternata infection better than 86-1 melon. After inoculation with A. alternata, the C4H, PAL, and 4CL activities of Jiashi and 86-1 melons peaked in the middle and late storage period, and the peak was higher in Jiashi melons. The gene expression changes were consistent with the enzyme activity. The C4H, PAL, and 4CL gene expression was significantly higher in Jiashi melon pericarp than in 86-1 melon, and the C4H, PAL, and 4CL activities in Jiashi melon were positively correlated with their gene expression, confirming the role of phenylpropanoid metabolism enzymes in resistance to A. alternata.

Transcriptomic analysis of developing embryos of apricot (Prunus armeniaca L.)

Yujia Bai,Weicheng Hu,Min Wang,Jing He,Yongxia Tao,Wei Huang,Zuoshan Feng 한국원예학회 2016 Horticulture, Environment, and Biotechnology Vol.57 No.2

Apricot (Prunus armeniaca L.) is an important, nutritionally valuable agricultural crop. A scarcity of transcriptomic and genomic data hinders progress toward understanding the molecular events of apricot embryogenesis, which is the primary determinant of fruit yield and kernel quality. In this study, three cDNA libraries were sequenced using an Illumina/Solexa platform. We obtained 35,928,496, 40,656,940, and 35,970,214 clean reads from endosperm formation (LT-A), embryo development (LT-B), and mature embryo (LT-C) cDNA libraries, respectively. De novo assembly of cDNA sequence data generated 35,614 unigenes with an average length of 973 bp. A total of 29,971 unigenes were matched in Nr, Nt, Swiss-Prot, KEGG, GO, and COG databases. The top matching species in the Nr database was P. persica (88.3% of matched Nr database accessions). A total of 8,327 differentially expressed genes (DEGs) were detected between LT-A and LT-B, of which 3,663 were upregulated and 4,664 were downregulated. Between LT-B and LT-C, 12,673 DEGs were detected, while 13,892 DEGs were uncovered between LT-A and LT-C. This work is the first reported application of next-generation sequencing techniques for transcriptome analysis of apricot embryo development. Besides providing valuable information on apricot embryo development, the comprehensive set of transcript sequences generated in this study may serve as additional molecular resources for the development of apricot functional genomics.

Differential Protein Expression Profiling in Pleurotus ferulae Mycelium Caused by Asafoetida Extracts Using a Proteomics Approach

Bai, Yujia,Huang, Wenshu,Tao, Yongxia,Feng, Zuoshan The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.1

The precise mechanism by which asafoetida extract influenced the growth of Pleurotus ferulae mycelium was investigated using a comparative proteomic analysis approach. Two types of asafoetida extracts were added to the medium of P. ferulae, and the effects of the extract on microstructure of P. ferulae mycelium were observed. Compared to the control group, the two asafoetida extracts played a significant role in promoting the growth of P. ferulae. Two-dimensional electrophoresis and LC-MS/MS were performed to examine changes in the expression of proteins in the mycelium of P. ferulae. A total of eight differentially expressed proteins were identified, including a heat shock protein, a flavin-containing monooxygenase, and a NADPH: quinone oxidoreductase type IV, as well as hypothetical and unknown proteins. Expression change of these proteins confirmed that asafoetida extracts significantly affected P. ferulae growth, metabolism, and secondary product metabolism. These results provide new insights into biochemical mechanisms underlying the relationship between P. ferulae and asafoetida.

A Comprehensive Evaluation Method for Reliability Confidence Capacity of Renewable Energy Based on Improved DEMATEL-AHP-EWM

Bai Xueyan,Fan Yanfang,Hou Junjie,Liu Yujia 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.3

With the continuous increase in the proportion of renewable energy access, reliability confdence capacity, as an important decision-making reference for measuring the contribution of power sources to the power system, has received widespread attention in power system planning and operation. It is of great signifcance to scientifcally and reasonably evaluate the reliability confdence capacity of renewable energy. This paper proposes a comprehensive evaluation method for renewable energy reliability confdence capacity based on improved Decision-Making Trial and Evaluation Laboratory-Analytic Hierarchy Process-Entropy Weight Method. Firstly, construct a reliability confdence capacity assessment model for renewable energy. Secondly, based on the comprehensive infuence weight calculated by improving the DEMATEL method, the AHPEWM subjective and objective weighting method based on game theory is used to establish the renewable energy confdence capacity index system and calculate the weight. Finally, simulation verifcation is conducted using an actual power grid in the northwest region of China as an example. The simulation results demonstrate that the proposed method has good feasibility and accuracy, and can provide decision-making support for the planning and operation of the new power system.

Effect of postharvest dehydration on the microstructure and anthocyanin content of grapes

Wancai Zheng,Aygul Alim,Yujia Bai,Zuoshan Feng,Jianmei Zhang,Na Xia,Zeren Ding 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.3

Postharvest dehydration temperature is important for the quality of grapes, especially the content of anthocyanin. Therefore,this article investigated the changes in the contents of total phenolic and anthocyanin compounds as well as the tissue microstructureof grapes. Furthermore, the correlation between the drying temperature and the total phenolic and anthocyanincompounds was analyzed. The results showed that the total phenol content was 1188.25 ± 37.32 mg/kg in the control groupand 998.13 ± 52.28 mg/kg and 886.00 ± 12.33 mg/kg in the groups treated at 25 °C and 45 °C, respectively, with 60% massloss. The microstructure of the grape peel showed shrinkage, rupture, and collapse with the disappearance of fi ne structuresduring dehydration at diff erent temperatures. The drying temperature was positively correlated with the dehydration rate( p < 0.01, sig = 0.781), which was negatively correlated with the total phenolic content ( p < 0.01, sig = − 0.985). Overall, theresults provide a reliable scientifi c basis for the extraction and utilization of anthocyanins in grapes.

Heart Failure Is Associated with Increased Risk of Long-Term Venous Thromboembolism

Tianyu Xu,Yuli Huang,Zuheng Liu,Yujia Bai,Zhuang Ma,Xiaoyan Cai,Yuhui Zhang,Jian Zhang 대한심장학회 2021 Korean Circulation Journal Vol.51 No.9

Background and Objectives: Venous thromboembolism (VTE), consisting of deep vein thrombosis (DVT) and pulmonary embolism (PE), is highly prevalent in in-hospital HF patients and contributes to worse prognoses. However, the risk of VTE in out-patients with HF in long-term period is controversial. This study aimed to evaluate the associations between HF and the risk of VTE in a long-term follow-up duration. Methods: We searched for studies investigating the risk of VTE, PE, and DVT in patients with HF before April 15, 2020, in PubMed, MEDLINE, and Embase databases. Cohort studies and post hoc analysis of RCTs were eligible for inclusion if they reported relative risk of VTE, DVT or PE in patients with HF in more than 3-month follow-up period. Results: We identified 31 studies that enrolled over 530,641 HF patients. Overall, patients with HF were associated with an increased risk of VTE (risk ratio [RR]=1.57, 95% confidence interval [CI]=1.34–1.84) and PE (RR=2.00, 95% CI=1.38–2.89). However, the risk of DVT was not significantly increased in HF patients (RR=1.33, 95% CI=0.67–2.63). Subgroup analysis showed that patients with chronic HF (RR=1.54, 95% CI=1.32–1.80) had a higher risk of VTE than those with acute HF (RR=0.95, 95% CI=0.68–1.32). Conclusions: In conclusion, HF was an independent risk for VTE and PE but not DVT in a long-term follow-up period. Patients with chronic HF were prone to suffer from VTE than acute HF.

Proteomic analyses on the browning of shade-dried Thompson seedless grape

Liu Fengjuan,Huang Wenshu,Feng Zuoshan,Tao Yongxia,Fan Yingying,He Weizhong,Li XiaoLi,Fang Xiaotong,Wang Cheng,Bai Yujia 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.3

China is one of the main producers in the worldwide raisin market. Most China’s raisins are produced in Xinjiang where the Thompson seedless grape ( Vitis vinifera L.cv.Thompson seedless) is the main variety of green raisin. However, the browning of Thompson seedless grape during drying has been well-acknowledged as the primary factor affecting the development of the raisin industry. Data independent acquisition (DIA)-based protein profiling was performed on fresh and shade-dried Thompson seedless grapes. As a result, 5431 proteins were identified, among which the amounts of 739 proteins in fresh grape were found to be significantly different with those in dried grape. The functional annotation based on the Blast2GO showed that the ‘organic substance metabolic process’, ‘regulation of molecular function’, ‘enzyme regulator activity’, and ‘isomerase activity’ related proteins became very active during browning. Further analyses revealed that the browning-related proteins, which with significant different amounts in fresh and in dried grapes, are primarily involved in the phenylpropanoid biosynthesis, tyrosine metabolism, phenylalanine metabolism, oxidative phosphorylation metabolism, plutathione metabolism, peroxisome pathway, and fatty acid degradation. And five random differential proteins were verified with parallel reaction monitoring (PRM). The PRM results were in agreement with the DIA data. The main browning-related proteins of Thompson seedless grape were identified in this study. Their properties were tested, and their roles in the browning mechanism were indicated. This will lay base to a better understanding on the enzymatic browning of Thompson seedless grape, and it will also provide guidance for controlling the quality of Thompson seedless grapes in industry.