Distinct Transcriptional and Functional Differences of Lung Resident and Monocyte-Derived Alveolar Macrophages During the Recovery Period of Acute Lung Injury

Hou Fei,Wang Huan,Zheng Kun,Yang Wenting,Xiao Kun,Rong Zihan,Xiao Junjie,Li Jing,Cheng Baihe,Tang Li,Xie Lixin 대한면역학회 2023 Immune Network Vol.23 No.3

In acute lung injury, two subsets of lung macrophages exist in the alveoli: tissue-resident alveolar macrophages (AMs) and monocyte-derived alveolar macrophages (MDMs). However, it is unclear whether these 2 subsets of macrophages have different functions and characteristics during the recovery phase. RNA-sequencing of AMs and MDMs from the recovery period of LPS-induced lung injury mice revealed their differences in proliferation, cell death, phagocytosis, inflammation and tissue repair. Using flow cytometry, we found that AMs showed a higher ability to proliferate, whereas MDMs expressed a larger amount of cell death. We also compared the ability of phagocytosing apoptotic cells and activating adaptive immunity and found that AMs have a stronger ability to phagocytose, while MDMs are the cells that activate lymphocytes during the resolving phase. By testing surface markers, we found that MDMs were more prone to the M1 phenotype, but expressed a higher level of pro-repairing genes. Finally, analysis of a publicly available set of single-cell RNA-sequencing data on bronchoalveolar lavage cells from patients with SARS-CoV-2 infection validated the double-sided role of MDMs. Blockade of inflammatory MDM recruitment using CCR2−/− mice effectively attenuates lung injury. Therefore, AMs and MDMs exhibited large differences during recovery. AMs are long-lived M2-like tissue-resident macrophages that have a strong ability to proliferate and phagocytose. MDMs are a paradoxical group of macrophages that promote the repair of tissue damage despite being strongly pro-inflammatory early in infection, and they may undergo cell death as inflammation fades. Preventing the massive recruitment of inflammatory MDMs or promoting their transition to pro-repairing phenotype may be a new direction for the treatment of acute lung injury.

Baicalein facilitates gastric cancer cell apoptosis by triggering endoplasmic reticulum stress via repression of the PI3K/AKT pathway

Shen Junjie,Yang Zhiwen,Wu Xinlin,Yao Guodong,Hou Mingxing 한국응용생명화학회 2023 Applied Biological Chemistry (Appl Biol Chem) Vol.66 No.-

Objective Gastric cancer (GC) remains a prevailing threat to life. Baicalein exhibits anti-cancer properties. This study estimated the mechanism of baicalein in GC cell apoptosis by mediating endoplasmic reticulum stress (ERS) through the PI3K/AKT pathway. Methods After treatment with different concentrations of baicalein, GC cell (HGC-27 and AGS) viability was detected by MTT assay. AGS cells more sensitive to baicalein treatment were selected as study subjects. The IC50 of baicalein on AGS cells was determined. Colony formation, cell cycle, and apoptosis were detected using crystal violet staining and flow cytometry. Levels of ERS-related and BTG3/PI3K/AKT pathway-related proteins were determined by Western blot. Intracellular Ca2+ level was measured using Fluo-3 AM fluorescence working solution. GC mouse models were established by subcutaneously injecting AGS cells into the right rib and were intragastrically administrated with baicalein. Tumor volume and weight were recorded. Expression of Ki67 in tumor tissues and positive expression of apoptotic cells were detected by immunohistochemistry and TUNEL staining. Results Baicalein inhibited cell proliferation and induced G0/G1 arrest and apoptosis by regulating the cell cycle, and triggered ERS in GC cells. Baicalein impeded the PI3K/AKT pathway by activating BTG3, thereby triggering ERS and inducing apoptosis. BTG3 inhibition reversed baicalein-induced apoptosis and ERS. Baicalein regulated GC cells in a concentration-dependent manner. Moreover, in xenograft mice, baicalein prevented tumor growth, decreased Ki67- positive cells, activated BTG3, and inhibited the PI3K/AKT pathway, thus activating ERS and increasing apoptotic cells. Conclusion Baicalein facilitates GC cell apoptosis by triggering ERS via repression of the PI3K/AKT pathway.

Effect of Resin Viscosity and Lubricants on Surface and Mechanical Properties of Glass Fiber Reinforced Polymer in Rapid Heat Cycle Molding

Aimin Zhang,Yang Hui,Junji Hou 한국고분자학회 2018 폴리머 Vol.42 No.6

Conventional injection molding (CIM) and rapid heat cycle molding (RHCM) methods are used to prepare ABS and (acrylonitrile-butadiene-styrene)/glass fiber copolymer (ABS/GF) plastic parts. Pentaerythritol stearate (PETS) and silicon particles are chosen as lubricants. Effect of resin viscosity and lubricants on properties of plastic parts was investigated. The results show that resin viscosity has some effect on surface quality of plastic parts in CIM process but no effect in RHCM process. ABS/GF plastic parts molded in RHCM process exhibit higher tensile, higher flexural strength and lower impact strength. Silicon is more effective in RHCM process than in CIM process.

A Comprehensive Evaluation Method for Reliability Confidence Capacity of Renewable Energy Based on Improved DEMATEL-AHP-EWM

Bai Xueyan,Fan Yanfang,Hou Junjie,Liu Yujia 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.3

With the continuous increase in the proportion of renewable energy access, reliability confdence capacity, as an important decision-making reference for measuring the contribution of power sources to the power system, has received widespread attention in power system planning and operation. It is of great signifcance to scientifcally and reasonably evaluate the reliability confdence capacity of renewable energy. This paper proposes a comprehensive evaluation method for renewable energy reliability confdence capacity based on improved Decision-Making Trial and Evaluation Laboratory-Analytic Hierarchy Process-Entropy Weight Method. Firstly, construct a reliability confdence capacity assessment model for renewable energy. Secondly, based on the comprehensive infuence weight calculated by improving the DEMATEL method, the AHPEWM subjective and objective weighting method based on game theory is used to establish the renewable energy confdence capacity index system and calculate the weight. Finally, simulation verifcation is conducted using an actual power grid in the northwest region of China as an example. The simulation results demonstrate that the proposed method has good feasibility and accuracy, and can provide decision-making support for the planning and operation of the new power system.

Capacitor commutation type DC circuit breaker with fault character discrimination capability

Yao Sun,Yanfang Fan,Junjie Hou 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.6

Flexible direct current (DC) grids face a serious challenge in terms of rapidly isolating DC faults. A DC circuit breaker is an effective solution for DC fault isolation. To improve the fault-isolation and reclosing capability of fl exible DC systems, a new high voltage direct current (HVDC) circuit breaker topology with adaptive reclosing capability is proposed in this paper. The topology of the circuit breaker is a T-shaped structure, which has the ability to break the current in both directions and effectively reduce the cost of components. Meanwhile, after the fault is cleared, the circuit breaker is controlled to inject a voltage signal into the line. Based on this, to prevent the circuit breaker reclosing in the event of a permanent fault from having an impact on the system, a method for fault identification based on Euclidean distance that uses the voltage signal to identify the fault properties is proposed. Finally, the performance of the circuit breaker is simulated and verified by PSCAD/EMTDC simulation software, and compared with the typical existing circuit breakers to verify the effectiveness of the circuit breaker and reclosing scheme.

Crystallization and Mechanical Properties of Glass Fiber Reinforced Polypropylene Composites Molded by Rapid Heat Cycle Molding

Aimin Zhang,Guoqun Zhao,Jialong Chai,Junji Hou,Chunxia Yang,Guilong Wang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.12

The crystalline behavior and mechanical properties of PP/GF (glass fibers) composites molded by rapid heat cyclemolding (RHCM) and conventional injection molding (CIM) were compared. SEM, DSC and XRD were utilized to studycrystallization behavior of PP and PP/GF composites. Furthermore, universal testing machine was employed to investigatethe mechanical properties. Results proved that higher degree of crystallinity and larger crystal size can be obtained in RHCMin comparison to CIM. GF can induce more crystal nuclei and then reduce the crystal size due to shear stress which isgenerated in polymer matrix around fibers. Nucleating agent (NA) has a positive effect on refine grains. The average crystaldiameter of PP/NA/30 %GF is about 1.7 μm which is one-tenth of PP/30 %GF (14 μm) in RHCM. XRD tests illustrated thatα-form crystal is the main crystal type for PP and PP/GF composites in RHCM and CIM. However, there is a little β-formcrystal in RHCM for PP/GF composites without NA. NA accelerates the formation of α-form crystal and restrains theemergence of β-form crystal. The plastic parts obtained in RHCM exhibited higher strength and modulus compared with thatobtained in CIM for both tensile and flexural tests.