A Gene Regulatory Network for Root Epidermis Cell Differentiation in Arabidopsis

Bruex, Angela,Kainkaryam, Raghunandan M.,Wieckowski, Yana,Kang, Yeon Hee,Bernhardt, Christine,Xia, Yang,Zheng, Xiaohua,Wang, Jean Y.,Lee, Myeong Min,Benfey, Philip,Woolf, Peter J.,Schiefelbein, John Public Library of Science 2012 PLoS genetics Vol.8 No.1

<▼1><P>The root epidermis of Arabidopsis provides an exceptional model for studying the molecular basis of cell fate and differentiation. To obtain a systems-level view of root epidermal cell differentiation, we used a genome-wide transcriptome approach to define and organize a large set of genes into a transcriptional regulatory network. Using cell fate mutants that produce only one of the two epidermal cell types, together with fluorescence-activated cell-sorting to preferentially analyze the root epidermis transcriptome, we identified 1,582 genes differentially expressed in the root-hair or non-hair cell types, including a set of 208 “core” root epidermal genes. The organization of the core genes into a network was accomplished by using 17 distinct root epidermis mutants and 2 hormone treatments to perturb the system and assess the effects on each gene's transcript accumulation. In addition, temporal gene expression information from a developmental time series dataset and predicted gene associations derived from a Bayesian modeling approach were used to aid the positioning of genes within the network. Further, a detailed functional analysis of likely bHLH regulatory genes within the network, including <I>MYC1</I>, <I>bHLH54</I>, <I>bHLH66</I>, and <I>bHLH82</I>, showed that three distinct subfamilies of bHLH proteins participate in root epidermis development in a stage-specific manner. The integration of genetic, genomic, and computational analyses provides a new view of the composition, architecture, and logic of the root epidermal transcriptional network, and it demonstrates the utility of a comprehensive systems approach for dissecting a complex regulatory network.</P></▼1><▼2><P><B>Author Summary</B></P><P>A current challenge in the field of developmental biology is to define the composition and organization of gene networks that direct the pattern and differentiation of cells, tissues, and organs. In this study, we address this problem using Arabidopsis root epidermis development, a relatively simple model for studies of cell pattern formation and differentiation in plants. We used a tissue-specific cell sorting approach to define more than 1,500 genes whose transcripts differentially accumulate in the developing root epidermis. A series of transcriptome analyses were performed with 17 root epidermal mutants and 2 plant hormone treatments to dissect the regulatory relationships between 208 core genes. In addition, gene expression information from a developmental time series dataset was used to organize genes temporally. The results provide insight into the composition, organization, and logic of a developmental gene regulatory network. Furthermore, this work demonstrates the utility of an integrated analysis in gene regulatory network construction using genetic, genomic, and computational approaches.</P></▼2>

MANAGING CULTURAL DISTINCTIVENESS WITHIN HOME SWAPPING

Nicole Ye Yang,Angela Paladino 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07

Consumers actively seek out authentic cultural experiences both in everyday consumption and when they travel. In this study, I investigate the processes through which personal homes are shared cross-culturally. I incorporate insights from the home and sharing literature to explore how consumers negotiate cultural distinctiveness and in-group boundaries when sharing their most valuable possessions. Using in-depth interviews, online archival data, and home photos, I find a swapping community where swappers share an in-group identity and enact loosely-defined conventions. Using these loosely-defined conventions, swappers negotiate a good working order that transcends cultural distinctiveness (Torelli, Ahluwalia, Cheng, Olson, & Stoner, 2017). As swappers localize these conventions in individual swap trades, they justify and temporarily tolerate the cultural distinctiveness. Or more experienced swappers normalize them as authentic experience. This research contributes to understanding how consumers actively negotiate cultural differences and authentic experiences in the sharing economy.

Characteristics of Sham/Placebo Acupuncture Controlled Trials for Headache

정수형,서정철,Angela Yang,Charlie Xue 대한침구의학회 2009 대한침구의학회지 Vol.26 No.1

목적 : 두통의 침술효과에 대한 연구가 꾸준히 이루어지고 있다. 하지만 많은 연구들에서 서로 상충되는 결과를 내놓고 있다. 이에 저자는 대조군 집단 시술의 역할과 시험의 특성에 대해 연구하는 데 있어 몇 가지 유형의 두통, 특히 긴장성 두통(Tension-type headache, TTH)과 편두통에 대한 침술의 대조군 시험에서 나타나는 일관성 없는 발견 사항들의 원인이 무엇인지에 대해 밝히려고 하였다. 방법 : 두통의 침술효과에 대해 보고하고 있는 11개의 임의대조군 시험(Randomized controlled trials, RCTs)을 대상으로 하여 위약 침술과 방법론적 특성 범주 III과 IV를 중심으로 위약 시술 분석, 비교를 실시하였다. 결과 : 임상적 유효성과의 상관관계에 있어 범주 III과 IV 사이에는 유의한 차이가 없었다. 표본 크기 계산, 표본 전체 크기, 진짜 집단과 위약 집단에 속한 피험자의 수에는 긍정적 결과와 중립적 결과 사이에 유의한 차이가 없었다. 결론 : 두통의 임의대조군 시험에 대한 다양한 유형의 위약 대조군과 침술의 방법론적 특성이 임상적 효과를 예측해 주는지의 여부에 대해 추가 연구가 이루어져야 하겠다.

FACILITATING NEGOTIATIONS IN AGENT MEDIATED ELECTRONIC COMMERCE

Chun Yan Miao,Angela Goh,Zhong Hua Yang 한국지능정보시스템학회 2001 한국지능정보시스템학회 기타 간행물 Vol.- No.-

Herbal formula (Danggui Beimu Kushen Wan) for prostate disorders: a systematic review of classical literature

Hong Li,Andrew Hung,Angela Wei Hong Yang 한국한의학연구원 2019 Integrative Medicine Research Vol.8 No.4

Background: Danggui Beimu Kushen Wan (DBKW) was initially known for difficult urination in pregnancy and has been widely used for prostate disorders in modern days. This study aimed to comprehensively investigate the implications of DBKW in traditional evidence. Methods: The Encyclopedia of Traditional Chinese Medicine was searched to identify the ingredients, dosage, etiologies, pathogeneses, actions and indications related to DBKW documented in ancient books. Descriptive summary was provided to their characteristics. Results: A total of 41 texts in 36 classic books were included. Two etiologies and 10 pathogeneses were investigated. All the identified formulas contain Angelicae Sinensis Radix, Fritillariae Thunbergii Bulbus and Sophorae Flavescentis Radix with the ratio of 1:1:1. The treatment dosage is three to 10 pills each time. The primary indication of DBKW is difficult urination with heat stagnation. Nine included texts specified that this formula could also be used for male. Conclusion: Included classic literature has provided fundamental evidence for the management of difficult urination in female and male. Further studies should investigate its mechanisms of actions for difficult urination related conditions, such as prostate disorders.

Quantification of airway diameters and 3D airway tree rendering from dynamic hyperpolarized ³He magnetic resonance imaging

Lewis, Tina A.,Tzeng, Yang-Sheng,McKinstry, Erin L.,Tooker, Angela C.,Hong, Kwansoo,Sun, Yanping,Mansour, Joey,Handler, Zachary,Albert, Mitchell S. Wiley Subscription Services, Inc., A Wiley Company 2005 Magnetic resonance in medicine Vol.53 No.2

<P>As another step toward extracting quantitative information from hyperpolarized <SUP>3</SUP>He MRI, airway diameters in humans were measured from projection images and multislice images of the lungs. Values obtained were in good agreement with the Weibel lung morphometry model. The measurement of airway caliber can now be achieved without the use of ionizing radiation. Furthermore, it was demonstrated that 3D airway tree renderings could be constructed from the multislice data. Both the measurement of airway diameters and the rendering of 3D airway information hold promise for the clinical assessment of bronchoconstrictive diseases such as asthma and the associated evaluation of treatment effectiveness. Work is being done to address the uncertainties of the manually intensive methods we have developed. Magn Reson Med 53:474–478, 2005. © 2005 Wiley-Liss, Inc.</P>

Characteristics of Sham/Placebo Acupuncture Controlled Trials for Headache

Jung, Su-Hyung,Seo, Jung-Chul,Yang, Angela,Xue, Charlie The Korean Acupuncture Moxibustion Medicine Societ 2009 대한침구의학회지 Vol.31 No.2

목적 : 두통의 침술효과에 대한 연구가 꾸준히 이루어지고 있다. 하지만 많은 연구들에서 서로 상충되는 결과를 내놓고 있다. 이에 저자는 대조군 집단 시술의 역할과 시험의 특성에 대해 연구하는 데 있어 몇 가지 유형의 두통, 특히 긴장성 두통(Tension-type headache, TTH)과 편두통에 대한 침술의 대조군 시험에서 나타나는 일관성 없는 발견 사항들의 원인이 무엇인지에 대해 밝히려고 하였다. 방법 : 두통의 침술효과에 대해 보고하고 있는 11개의 임의대조군 시험(Randomized controlled trials, RCTs)을 대상으로 하여 위약 침술과 방법론적 특성 범주 III과 IV를 중심으로 위약 시술 분석, 비교를 실시하였다. 결과 : 임상적 유효성과의 상관관계에 있어 범주 III과 IV 사이에는 유의한 차이가 없었다. 표본 크기 계산, 표본 전체 크기, 진짜 집단과 위약 집단에 속한 피험자의 수에는 긍정적 결과와 중립적 결과 사이에 유의한 차이가 없었다. 결론 : 두통의 임의대조군 시험에 대한 다양한 유형의 위약 대조군과 침술의 방법론적 특성이 임상적 효과를 예측해 주는지의 여부에 대해 추가 연구가 이루어져야 하겠다.

3D surface mapping of freeform optics using wavelength scanning lateral shearing interferometry.

Ghim, Young-Sik,Rhee, Hyug-Gyo,Davies, Angela,Yang, Ho-Soon,Lee, Yun-Woo Optical Society of America 2014 Optics express Vol.22 No.5

<P>Freeform optics have emerged as promising components in diverse applications due to the potential for superior optical performance. There are many research fields in the area ranging from fabrication to measurement, with metrology being one of the most challenging tasks. In this paper, we describe a new variant of lateral shearing interferometer with a tunable laser source that enables 3D surface profile measurements of freeform optics with high speed, high vertical resolution, large departure, and large field-of-view. We have verified the proposed technique by comparing our measurement result with that of an existing technique and measuring a representative freeform optic.</P>

Epigenetically regulated MIR941 and MIR1247 target gastric cancer cell growth and migration

Kim, Joong-Gook,Kim, Tae-Oh,Bae, Jin-Han,Shim, Jae-Woong,Kang, Myoung Joo,Yang, Kwangmo,Ting, Angela H,Yi, Joo Mi LANDERS BIOSCIENCE 2014 Epigenetics Vol.9 No.7