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RISS 인기검색어
- 검색키워드
- 저자 : Anchun Chen (검색결과 3 건)
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Apoptosis induced in vivo by new type gosling viral enteritis virus
Shun Chen,Anchun Cheng,Mingshu Wang,Dekang Zhu,Renyong Jia,Qihui Luo,Hengmin Cui,Yi Zhou,Yin Wang,Zhiwen Xu,Zhengli Chen,Xiaoyue Chen,Xiaoyu Wang 대한수의학회 2011 JOURNAL OF VETERINARY SCIENCE Vol.12 No.4
In this study, apoptosis was induced by new type gosling viral enteritis virus (NGVEV) in experimentally infected goslings is reported in detail for the first time. After 3-day-old goslings were orally inoculated with a NGVEV-CN strain suspension, the time course of NGVEV effects on apoptotic morphological changes of the internal tissues was evaluated. These changes were observed by histological analysis with light microscopy and ultrastructural analysis with transmission electron microscopy. DNA fragmentation was assessed with a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay and DNA ladder analysis. A series of characteristic apoptotic morphological changes including chromatin condensation and margination, cytoplasmic shrinkage, plasma membrane blebbing, and formation of apoptotic bodies were noted. Apoptosis was readily observed in the lymphoid and gastrointestinal organs, and sporadically occurred in other organs after 3 days post-infection (PI). The presence and quantity of TUNEL-positive cells increased with infection time until 9 days PI. DNA extracted from the NGVEV-infected gosling cells displayed characteristic 180~200 bp ladders. Apoptotic cells were ubiquitously distributed, especially among lymphocytes, macrophages, monocytes, and epithelial and intestinal cells. Necrosis was subsequently detected during the late NGVEV-infection phase, which was characterized by cell swelling, plasma membrane collapse, and rapidly lysis. Our results suggested that apoptosis may play an important role in the pathogenesis of NGVE disease.
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An analytical model for PVC-FRP confined reinforced concrete columns under low cyclic loading
Yuan Fang,Feng Yu,Anchun Chen,Shilong Wang,Guoshi Xu 국제구조공학회 2021 Structural Engineering and Mechanics, An Int'l Jou Vol.77 No.2
Experimental investigations on the seismic behaviors of the PVC-FRP Confined Reinforced Concrete (PFCRC) columns under low cyclic loading are carried out and two variable parameters including CFRP strips spacing and axial compression ratio are considered. The PFCRC column finally fails by bending and is characterized by the crushing of concrete and yielding of the longitudinal reinforcement, and the column with a high axial compression ratio is also accompanied by the cracking of the PVC tube and the fracture of CFRP strips. The hysteretic curves and skeleton curves of the columns are obtained from the experimental data. With the increase of axial compression ratio, the stiffness degradation rate accelerates and the ductility decreases. With the decrease of CFRP strips spacing, the unloading sections of the skeleton curves become steep and the ductility reduces significantly. On the basis of fiber model method, a numerical analysis approach for predicting the skeleton curves of the PFCRC columns is developed. Additionally, a simplified skeleton curve including the elastic stage, strengthening stage and unloading stage is suggested depending on the geometric drawing method. Moreover, the loading and unloading rules of the PFCRC columns are revealed by analyzing the features of the skeleton curves. The quantitative expressions that are used to predict the unloading stiffness of the specimens in each stage are proposed. Eventually, an analytical model for the PFCRC columns under low cyclic loading is established and it agrees well with test data.
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Meng Lin,Renyong Jia,Mingshu Wang,Xinghong Gao,Dekang Zhu,Shun Chen,Mafeng Liu,Zhongqiong Yin,Yin Wang,Xiaoyue Chen,Anchun Cheng 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.3
The UL49.5 gene of most herpesviruses is conserved andencodes glycoprotein N. However, the UL49.5 protein ofduck enteritis virus (DEV) (pUL49.5) has not been reported. In the current study, the DEV pUL49.5 gene was firstsubjected to molecular characterization. To verify thepredicted intracellular localization of gene expression, therecombinant plasmid pEGFP-C1/pUL49.5 was constructedand used to transfect duck embryo fibroblasts. Next, therecombinant plasmid pDsRed1-N1/ glycoprotein M (gM)was produced and used for co-transfection with thepEGFP-C1/pUL49.5 plasmid to determine whether DEVpUL49.5 and gM (a conserved protein in herpesviruses)colocalize. DEV pUL49.5 was thought to be an envelopeglycoprotein with a signal peptide and two transmembranedomains. This protein was also predicted to localize in thecytoplasm and endoplasmic reticulum with a probability of66.7%. Images taken by a fluorescence microscope atdifferent time points revealed that the DEV pUL49.5 andgM proteins were both expressed in the cytoplasm. Overlapof the two different fluorescence signals appeared 12 h aftertransfection and continued to persist until the end of theexperiment. These data indicate a possible interaction between DEV pUL49.5 and gM.
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