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YouTube videos provide low-quality educational content about rotator cuff disease
Kyle N. Kunze,Kevin H. Alter,Matthew R. Cohn,Amar S. Vadhera,Nikhil N. Verma,Adam B. Yanke,Jorge Chahla 대한견주관절의학회 2022 대한견주관절의학회지 Vol.25 No.3
Background: YouTube has become a popular source of healthcare information in orthopedic surgery. Although quality-based studies of YouTube content have been performed for information concerning many orthopedic pathologies, the quality and accuracy of information on the rotator cuff have yet to be evaluated. The purpose of the current study was to evaluate the reliability and educational content of YouTube videos concerning the rotator cuff. Methods: YouTube was queried for the term “rotator cuff.” The first 50 videos from this search were evaluated. Video reliability was assessed using the Journal of the American Medical Association (JAMA) benchmark criteria (range, 0–5). Educational content was assessed using the global quality score (GQS; range, 0–4) and the rotator cuff-specific score (RCSS; range, 0–22). Results: The mean number of views was 317,500.7±538,585.3. The mean JAMA, GQS, and RCSS scores were 2.7±2.0, 3.7±1.0, and 5.6±3.6, respectively. Non-surgical intervention content was independently associated with a lower GQS (β=–2.19, p=0.019). Disease-specific video content (β=4.01, p=0.045) was the only independent predictor of RCSS. Conclusions: The overall quality and educational content of YouTube videos concerned with the rotator cuff were low. Physicians should caution patients in using such videos as resources for decision-making and should counsel them appropriately.
Prapas Patchanee,Pakpoom Tadee,Orapun Arjkumpa,David Love,Karoon Chanachai,Thomas Alter,Soawapak Hinjoy,Prasit Tharavichitkul 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.4
This study was conducted to determine the prevalence oflivestock-associated methicillin-resistant Staphylococcus aureus(LA-MRSA) in pigs, farm workers, and the environment innorthern Thailand, and to assess LA-MRSA isolatephenotypic characteristics. One hundred and four pig farmswere randomly selected from the 21,152 in Chiang Mai andLamphun provinces in 2012. Nasal and skin swab sampleswere collected from pigs and farm workers. Environmentalswabs (pig stable floor, faucet, and feeder) were also collected. MRSA was identified by conventional bacterial culturetechnique, with results confirmed by multiplex PCR and multilocus sequence typing (MLST). Herd prevalence of MRSA was9.61% (10 of 104 farms). Among pigs, workers, and farmenvironments, prevalence was 0.68% (two of 292 samples),2.53% (seven of 276 samples), and 1.28% (four of 312 samples),respectively. Thirteen MRSA isolates (seven from workers,four from environmental samples, and two from pigs) wereidentified as Staphylococcal chromosomal cassette mec IVsequences type 9. Antimicrobial sensitivity tests found 100% ofthe MRSA isolates resistant to clindamycin, oxytetracycline, andtetracycline, while 100% were susceptible to cloxacillin andvancomycin. All possessed a multidrug- resistant phenotype. This is the first evidence of an LA-MRSA interrelationship among pigs, workers, and the farm environment in Thailand.
( Patchanee Prapas ),( Chomporn Chokboonmongkol ),( Karl Hans Zessin ),( Thomas Alter ),( Sarinya Pornaem ),( Nipa Chokesajjawatee ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.11
We compared rapid fingerprinting using repetitive sequencebased PCR (rep-PCR) for subtyping Campylobacter jejuni isolates to the widely used multilocus sequence typing (MLST). Representative C. jejuni isolates (n=16) from broilers were analyzed using MLST and rep-PCR. Both techniques demonstrated an equal discriminatory power of 0.8917, and 9 subgroups were identified. Clonal identification of all 16 isolates was identical for both techniques. The rep-PCR as described in this study may be used as a rapid and cost-effective alternative for subtyping of C. jejuni isolates, or as an effective screening tool in large epidemiological studies.
Schultz, Bruce T.,Teigler, Jeffrey E.,Pissani, F.,Oster, Alexander F.,Kranias, G.,Alter, G.,Marovich, M.,Eller, Michael A.,Dittmer, U.,Robb, Merlin L.,Kim, Jerome H.,Michael, Nelson L.,Bolton, D.,Stre Cell Press 2016 Immunity Vol.44 No.1
<P>A central effort in HIV vaccine development is to generate protective broadly neutralizing antibodies, a process dependent on T follicular helper (Tfh) cells. The feasibility of using peripheral blood counterparts of lymph node Tfh cells to assess the immune response and the influence of viral and vaccine antigens on their helper functions remain obscure. We assessed circulating HIV-specific IL-21(+) CD4(+) T cells and showed transcriptional and phenotypic similarities to lymphoid Tfh cells, and hence representing peripheral Tfh (pTfh) cells. pTfh cells were functionally active and B cell helper quality differed depending on antigen specificity. Furthermore, we found higher frequency of pTfh cells in peripheral blood mononuclear cell specimens from the ALVAC+AIDSVAX (RV144) HIV vaccine trial associated with protective antibody responses compared to the non-protective DNA+Ad5 vaccine trial. Together, we identify IL-21(+) CD4(+) T cells as pTfh cells, implicating them as key populations in the generation of vaccine-evoked antibody responses.</P>
Molecular Surveillance Identifies Multiple Transmissions of Typhoid in West Africa
Wong, Vanessa K.,Holt, Kathryn E.,Okoro, Chinyere,Baker, Stephen,Pickard, Derek J.,Marks, Florian,Page, Andrew J.,Olanipekun, Grace,Munir, Huda,Alter, Roxanne,Fey, Paul D.,Feasey, Nicholas A.,Weill, F Public Library of Science 2016 PLoS neglected tropical diseases Vol.10 No.9
<▼1><P><B>Background</B></P><P>The burden of typhoid in sub-Saharan African (SSA) countries has been difficult to estimate, in part, due to suboptimal laboratory diagnostics. However, surveillance blood cultures at two sites in Nigeria have identified typhoid associated with <I>Salmonella enterica</I> serovar Typhi (<I>S</I>. Typhi) as an important cause of bacteremia in children.</P><P><B>Methods</B></P><P>A total of 128 <I>S</I>. Typhi isolates from these studies in Nigeria were whole-genome sequenced, and the resulting data was used to place these Nigerian isolates into a worldwide context based on their phylogeny and carriage of molecular determinants of antibiotic resistance.</P><P><B>Results</B></P><P>Several distinct <I>S</I>. Typhi genotypes were identified in Nigeria that were related to other clusters of <I>S</I>. Typhi isolates from north, west and central regions of Africa. The rapidly expanding <I>S</I>. Typhi clade 4.3.1 (H58) previously associated with multiple antimicrobial resistances in Asia and in east, central and southern Africa, was not detected in this study. However, antimicrobial resistance was common amongst the Nigerian isolates and was associated with several plasmids, including the IncHI1 plasmid commonly associated with <I>S</I>. Typhi.</P><P><B>Conclusions</B></P><P>These data indicate that typhoid in Nigeria was established through multiple independent introductions into the country, with evidence of regional spread. MDR typhoid appears to be evolving independently of the haplotype H58 found in other typhoid endemic countries. This study highlights an urgent need for routine surveillance to monitor the epidemiology of typhoid and evolution of antimicrobial resistance within the bacterial population as a means to facilitate public health interventions to reduce the substantial morbidity and mortality of typhoid.</P></▼1><▼2><P><B>Author Summary</B></P><P>Typhoid fever, a serious bloodstream infection caused by the bacterium <I>Salmonella</I> Typhi, is a major cause of disease and death around the world. There have been limited data on the epidemiology of typhoid in many countries in sub-Saharan African, including Nigeria. Recent evidence, however, showed that typhoid was an important cause of bacteraemia in children residing in two regions of Nigeria. Here, we analyzed the whole genome sequences of 128 <I>S</I>. Typhi isolates from two studies in order to elucidate the population structure and characterize the genetic components of antimicrobial resistance. We found that the multiple <I>S</I>. Typhi genotypes identified were closely related to other <I>S</I>. Typhi from neighboring regions of Africa and that multidrug resistance (MDR) was common among these isolates, and in many cases was associated with the IncHI1 plasmid known to cause MDR typhoid. These results provide evidence that typhoid was established in Nigeria as a result of several independent introductions into the country and that there has been extensive exchange of <I>S</I>. Typhi in and around the region of West Africa. This study emphasizes the importance of surveillance to improve our understanding of the epidemiology of typhoid, which is needed to underpin public health measures to reduce the spread of disease and facilitate patient management.</P></▼2>