Biosurfactant production by the hydrocarbon-degrading bacteria (HDB) Serratia marcescens: Optimization using central composite design (CCD)

Asia Fadhile Almansoory,Hassimi Abu Hasan,Mushrifah Idris,Siti Rozaimah Sheikh Abdullah,Nurina Anuar,El Mubarak Musa Tibin 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.47 No.-

Based on the nutritional and physiological requirements of a native hydrocarbon-degrading bacterium(HDB), Serratia marcescens, an investigation on the potential biosurfactant production by this bacterialspecies was conducted. The effects of different carbon sources, nitrogen sources, salinity, pH,temperature, and agitation speeds were extensively studied. The optimal conditions of biosurfactantproduction and surface tension were determined using central composite design (CCD) by settingglycerol, peptone and ammonium sulphate (NH4)2SO4 in a range of 3–7%, 2–6 g/L and 3–7 g/L,respectively. The results showed that biosurfactant was highly produced at pH 8, a temperature of 30 C, asalinity of 1% and a speed of 200 rpm after 5 days (120 h) of incubation. The optimal conditions wereobtained at 5% glycerol, 4 g/L peptone and 5 g/L (NH4)2SO4 with a maximum biosurfactant production of1.42 g/L and a minimum surface tension of 28.4 mN/m. Thus, the HDB S. marcescens shows good potentialas a biosurfactant-producing bacterium to be used in any environmental application and as an alternativeto chemical surfactants.

Rabbit submandibular salivary gland replantation

Akram A. Almansoori,Namuun Khentii,Wei Hong Hei,Nari Seo,Sung Ho Lee,Soung Min Kim,Jong Ho Lee 대한구강악안면외과학회 2017 대한구강악안면외과학회지 Vol.43 No.5

Objectives: To test the feasibility of submandibular salivary gland (SMG) replantation techniques and the survival of the replanted glands. Such a study can provide a rationale for later allotransplantation procedures, along with implementation of conventional and advanced immunosuppression therapy. Materials and Methods: Six SMG replantations were performed in New Zealand white rabbits. One week postoperatively, 99mTc scintigraphy was performed and the uptake ratio and salivary excretion fraction were calculated. Two to four weeks later, submandibular glands were excised, fixed, and stained with H&E for histomorphometric evaluation. Results: Intraoperatively, all glands showed patent blood perfusion except gland 5. Positive tracer uptake and saliva excretion were documented by scintigraphy. On excision, all of the glands except glands 4 and 5 looked viable, with a red color and patent pedicles. Gland 4 was infected and filled with creamy pus, while gland 5 looked pale and necrotic. Histologically, glands 1, 2, 3, and 6 had preserved normal glandular tissue with slight variations from the contralateral normal glands, as their parenchyma was composed of mildly atrophic acini. Conclusion: Four out of six replanted SMGs successfully survived. The glands maintained good viability and function. Such success depends on safe harvesting, short anastomosis time, and strict control of infection.

Hydroxyapatite coated magnesium alloy for peripheral nerve regeneration

( Akram Abdo Almansoori ),( Kyung Won Ju ),( Bongju Kim ),( Soung Min Kim ),( Sung-mi Lee ),( Jong-ho Lee ) 조선대학교 치의학연구원(구 조선대학교 구강생물학연구소) 2018 Oral Biology Research (Oral Biol Res) Vol.42 No.3

Magnesium (Mg) has been used as a neuroprotective agent and for orthopedic and cardiovascular stent applications for a long time. Mg is a promising material for neural regeneration own to its metallic nature, biodegradability, and biocompatibility. To evaluate the benefit of hydroxyapatite (HA) coated Mg alloy (WE43) nerve conduit in peripheral nerve injury regeneration. In vitro cell adhesion and proliferation of PC12 cells and Schwann cells over pure Mg, WE43, HA coated pure Mg (HA-Mg), and HA coated WE43 (HA-WE43) disks were assessed using scanning electron microscope and EZ-CYTOX assay. In vivo study was also performed for sham, silicone nerve conduits, and HA-WE43 nerve conduits to evaluate rat sciatic nerve regeneration over 10 mm gap. These rats were observed for three months with weekly functional assessment using sciatic functional index. Regeneration was evaluated through retrograde neuron labeling and histomorphometric analysis at the end of the experiment. Non-coated pure Mg and WE43 conduits showed fast resorption rate and high gas formation. Higher adhesion and proliferation of PC12 cells were found in HA-WE43 disks. At the end of three months, HA-WE43 nerve conduit showed mild resorption without detectable gas formation in the surrounding tissue. However, only scanty regenerated neural tissue was found within the conduit. HA-WE43 nerve conduit showed controlled degradation and absence of gas formation with scanty regenerated neural tissue.

Maxillary Sinus Augmentation and Implant Installation with Commissuroplasty in a Patient with Burn-induced Microstomia

Akram Abdo Almansoori,이원욱,유수연,권익재 대한구강악안면임플란트학회 2023 대한구강악안면임프란트학회지 Vol.27 No.3

Microstomia is caused by congenital growth abnormalities, exposure to electricity or chemicals, or direct burns. In the case of burn-induced microstomia, treatment is difficult because of fibrosis at the burn site. During dental treatment, buccal retraction is necessary to access the posterior teeth. When performing maxillary sinus augmentation using the lateral approach, sufficient buccal retraction through the buccal vestibule is required. Implant placement with sinus augmentation in patients with burn-induced microstomia caused by is very difficult to treat. Herein, we describe the successful results of simultaneous bilateral sinus augmentation and implant placement along with commissuroplasty and upper

FK506 immunosuppression for submandibular salivary gland allotransplantation in rabbit

Akram Abdo Almansoori,Namuun Khentii,Kyung Won Ju,Bongju Kim,Soung Min Kim,Jong-Ho Lee 대한구강악안면외과학회 2020 대한구강악안면외과학회지 Vol.46 No.3

Objectives: We compared the outcomes of two different doses of FK506 (tacrolimus) for immunosuppression in submandibular salivary gland (SMG) allotransplantation. Materials and Methods: Three SMG allotransplantation groups were established (n=6 per group) as follows: allograft rejection control (Allo-Ctrl), low dose (0.08 mg/kg) of FK506 (FK506-L), and high dose (0.16 mg/kg) of FK506 (FK506-H). Allograft survival and rejection were assessed by clinical observation, interleukin-2 levels as determined by enzyme-linked immunosorbent assay, blood sampling for complete blood count (CBC), and histological evaluation. Results: Body weight and anorexia were higher in the FK506-H group but without a significant difference compared with the FK506-L population. CBC revealed a non-significantly reduced number of changes in the FK506-L group. Four glands in the FK506-H group and two glands in the FK506- L group were viable and functioning post-transplantation. Conclusion: The survival rate of allotransplanted glands was higher in conjunction with the high dose of 0.16 mg/kg of FK506, with no major difference in the side-effect profile when compared with the low dose of 0.08 mg/kg short-term outcomes.

Determinants intention usage of Islamic E-Wallet Among Millennials

Mohammed Hariri Bakri(Mohammed Hariri Bakri ),Khalifa Khalfan Salem Muftah Almansoori(Khalifa Khalfan Salem Muftah Almansoori ),Nur Syazni Mohd Azlan(Nur Syazni Mohd Azlan ) People&Global Business Association 2023 Global Business and Finance Review Vol.28 No.1

Purpose: The main purpose of this study is to investigate the behavioural intention of the millennials in Malaysia toward the acceptance of Islamic e-wallet usage using Unified Theory of Acceptance and Use of Technology(UTAUT) via Structural Equation Model. Design/methodology/approach: The data have been collected by the 384 millennials in Malaysia. A person has aged between 25 until 40 years old is considered the millennials group The method that used in this paper is the Unified theory of acceptance and use of technology (UTAUT). There will be five independent variables: performance expectancy, effort expectancy, social influence, facilitating condition, and Shariah-compliant. Shariah-compliant variables have been added to the study because the study investigates the Islamic e-wallet where it is needed the Shariah-compliant elements. Before the data is analysed into measurement, preliminary analysis such as Exploratory Factor Analysis (EFA) is conducted as well. Findings: The result showed that the performance expectancy (PE), effort expectancy (EE), social influence (SI) and Shariah complaint (SC) significantly affect the Islamic e-wallet acceptance among the millennials. In addition, the Shariah complaint has a strong effect on Islamic e-wallet acceptance. In contrast, facilitating condition (FC) showed no effect on Islamic e-wallet acceptance. In this study, all the requirements for model fit were achieved. The four exogenous constructs are performance expectancy (PE), effort expectancy (EE), social influence (SI) and facilitating condition (FC). The mediating construct is behavioural intention (BI) and the endogenous is user adoption (UA). All exogenous constructs show significant p-value except for effort expectancy (EE). Research limitation/implications: This paper offers important implications specifically for the digital economy as it is currently making its way throughout every aspect in the human life, be it social, religion, financial transaction, entertainment and many more. The impact of digital economy can be traced through the emergence of Fintech. One of the least academically discussed areas is the adoption of Islamic Fintech. This study is considered necessary to explore the prediction of consumer behaviour in Islamic Fintech intention, as part of digital economy. Originality/value: The paper fills the gap perceived within the existing literature of finance technology by showing Islamic financial technology (E-Wallet) intention via model measurement.

Per-oral cross-facial sural nerve graft for facial reanimation

Jeong, Joohee,Almansoori, Akram Abdo,Park, Hyun-Soo,Byun, Soo-Hwan,Min, Seung-Ki,Choung, Han-Wool,Park, Joo Yong,Choi, Sung Weon,Kim, Bongju,Kim, Soung-Min,Lee, Jong-Ho Korean Association of Maxillofacial Plastic and Re 2018 Maxillofacial Plastic Reconstructive Surgery Vol.40 No.-

Background: Cross-facial nerve graft is considered the treatment of choice for facial reanimation in patients with unilateral facial palsy caused by central facial nerve damage. In most cases, a traditional parotidectomy skin incision is used to locate the buccal and zygomatic branches of the facial nerve. Methods: In this study, cross-facial nerve graft with the sural nerve was planned for three patients with facial palsy through an intraoral approach. Results: An incision was made on the buccal cheek mucosa, and the dissection was performed to locate the buccal branch of the facial nerve. The parotid papillae and parotid duct were used as anatomic landmarks to locate the buccal branch. Conclusions: The intraoral approach is more advantageous than the conventional extraoral approach because of clear anatomic marker (parotid papilla), invisible postoperative scar, reduced tissue damage from dissection, and reduced operating time.

Leiomyosarcoma of the jaw: case series

Yong-Suk Choi,Akram Abdo Almansoori,Tae-Young Jung,Jae-Il Lee,Soung Min Kim,Jong-Ho Lee 대한구강악안면외과학회 2020 대한구강악안면외과학회지 Vol.46 No.4

Objectives: Leiomyosarcoma is a malignant neoplasm that affects smooth muscle tissue and it is very rare in the field of oral and maxillofcial surgery. The purpose of this study was to obtain information on diagnosis of and treatment methods for leiomyosarcoma by retrospectively reviewing of the cases. Patients and Methods: The study included nine patients who were diagnosed with leiomyosarcoma in the Department of Oral and Maxillofacial Surgery at Seoul National University Dental Hospital. The subjects were analyzed with respect to sex, age, clinical features, primary site of disease, treatment method, recurrence, and metastasis. Results: Particular clinical features included pain, edema, mouth-opening limitations, dysesthesia, and enlarged lymph nodes. All cases except one were surgically treated, and recurrence was found in two cases. Four of nine patients were followed up without recurrence and one patient underwent additional surgery due to recurrence. Conclusion: In our case series, notable symptoms included pain, edema, mouth-opening limitations, and dysesthesia; however, it was difficult to label these as specific symptoms of leiomyosarcoma. Considering the aggressive characteristics of the disease and poor prognosis, surgical treatment is necessary with careful consideration of postoperative radiotherapy and chemotherapy.

Sinus augmentation with poly(ε) caprolactone-β tricalcium phosphate scaffolds, mesenchymal stem cells and platelet rich plasma for one-stage dental implantation in minipigs

Jeong-Hun Nam,Akram Abdo Almansoori,Oh-Jun Kwon,Young-Kwon Seo,Bongju Kim,Young-Kyun Kim,Jong-Ho Lee,KangMi Pang Korean Academy of Periodontology 2023 Journal of Periodontal & Implant Science Vol.53 No.3

Purpose: This study evaluated the efficacy of a tube-shaped poly(ε) caprolactone - β tricalcium phosphate (PCL-TCP) scaffold with the incorporation of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) and platelet-rich plasma (PRP) for bone regeneration in the procedure of single-stage sinus augmentation and dental implantation in minipigs. Methods: Implants were placed in the bilateral sides of the maxillary sinuses of 5 minipigs and allocated to a PCL-TCP+hUCMSCs+PRP group (n=5), a PCL-TCP+PRP group (n=5), and a PCL-TCP-only group (n=6). After 12 weeks, bone regeneration was evaluated with soft X-rays, micro-computed tomography, fluorescence microscopy, and histomorphometric analysis. Results: Four implants failed (2 each in the PCL-TCP+hUCMSCs+PRP and PCLTCP+hUCMSC groups). An analysis of the grayscale levels and bone-implant contact ratio showed significantly higher mean values in the PCL-TCP+hUCMSCs+PRP than in the PCL-TCP group (P=0.045 and P=0.016, respectively). In fluoromicroscopic images, new bone formation around the outer surfaces of the scaffolds was observed in the PCLTCP+hUCMSCs+PRP group, suggesting a tenting effect of the specially designed scaffolds. Bone regeneration at the scaffold-implant interfaces was observed in all 3 groups. Conclusions: Using a tube-shaped, honeycombed PCL-TCP scaffold with hUCMSCs and PRP may serve to enhance bone formation and dental implants' osseointegration in the procedure of simultaneous sinus lifting and dental implantation.