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( Sakatoku akihiro ),( Daisuke Tanaka ),( Shogo Nakamura ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.6
In a previous study, we isolated and reported a second species of the Saccharophagus genus, Saccharophagus sp. strain Myt-1. In the present study, an alginate lyase gene (algMytC) from the genomic DNA of Myt-1 was cloned and characterized. The DNA sequence fragment obtained contained an open reading frame of 1,032 bp that encoded a protein of 343 amino acids with an estimated molecular mass of 37.6 kDa and a pI of 6.60. The deduced protein, AlgMytC, had the conserved amino acid sequences (RTELREM, QIH, YFKAGVYNQ) of the polysaccharide lyase family 7. A BLAST homology search indicated that AlgMytC shared an amino acid sequence identity of 95.9% with alg7A of S. degradans 2-40. The cloned and purified AlgMytC protein showed optimal activity at 40oC, and retained more than 90% of its total activity even after treatment at 25oC for 24 h. AlgMytC was very alkaliphilic with an optimal pH of 9.0, and more than 90% of its activity was retained in the pH range 8.5-10.0. Moreover, AlgMytC was stable over a wide pH range. The activity of AlgMytC was also stable in the presence of various detergents.
Akihiro Furube,Ryuzi Katoh,Yoshiaki Tamaki 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.47 No.1
We developed a femtosecond time-resolved scanning near-field optical microscope (SNOM) by combining a SNOM having an apertured atomic-force microscope cantilever tip with an amplified Ti:sapphire laser system. The time resolution of the apparatus was estimated to be less than 200 fs by measuring the autocorrelation function of 660-nm femtosecond laser pulses on the basis of a two-photon absorption method, even when the group-velocity dispersion of femtosecond pulses was not compensated.
4-Thiazinyl-1235-dithiadiazolyl cation: robustferromagnetic coupling against conformational change
Akihiro Ito,Masashi Urabe,Haruhiro Ino,Kazuyoshi Tanaka 한국물리학회 2005 Current Applied Physics Vol.5 No.2
Quantum chemical calculations employing two-conguration self-cosistent eld theory and density functional theory have beencarried out for a new diradical cation composed of two ring systems, 1,4-thiazine and 1,2,3,5-dithiadiazolyl. These ring systems arecoupled through a central CAN bond, and hence, the free rotation are allowed for this diradical cation. However, the singlettripletenergy splitting has been predicted to be positive for every dihedral angle between two ring systems. Moreover, the cation diradicalwas found to be isoelectronic with trimethylenemethane in the planar conformation. This fact is deeply related with the robustnessof high-spin correlation of the diradical cation..