Comparison of Proteome Components of Helicobacter pylori Before and After Mouse Passage

Lee, Kyoung-Ja,Kim, Bok-Ran,Cho, Young-A,Song, Yun-Gyu,Song, Jae-Young,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Cho, Myung-Je,Rhee, Kwang-Ho,Seo, Ji-Hyun,Youn, Hee-Shang,Lee, Woo-Kon 대한미생물학회 2011 Journal of Bacteriology and Virology Vol.41 No.4

The mouse model is alleged to be a useful tool for understanding of pathophysiological roles of Helicobacter pylori in the development of gastric disorders. However, it has been observed that H. pylori strains significantly differed in their fitness in mice and even mouse strains differed in their susceptibilities to a H. pylori strain. Bacterial components of H. pylori which could affect on its fitness in mice have to be elucidated for the establishment of the mouse model for H. pylori infections. In the comparison of colonization ability between two H. pylori Korean isolates, 51 (isolated from a patient with duodenal ulcer) and 52 (isolated from a patient with gastric cancer), 52 could colonize better than 51 on the gastric mucosa of mouse. Proteome components of H. pylori 52, as a good colonizer and H. pylori 51, as a poor one were quantitatively compared each other. Five bacterial proteins including catalase, urease subunit alpha/beta, enolase and ferritin, were up-regulated in 52. In addition, the respective proteome components of the two strains were also compared with their mouse-passaged homologous strains. Seven and five proteins, which included catalase, flagellin A/B in common, were up-regulated in mouse-adapted 51 and 52, respectively. Among the fourteen identified proteins, urease subunit alpha/beta, flagellin A/B, catalase, ferritin, superoxide dismutase and neutrophil-activation protein have been previously known to be necessary to gastric colonization of H. pylori in animal models. The other up-regulated proteins including enolase, elongation factor Tu and fructose-bisphosphate aldolase have been reported to be associated with acid tolerance of H. pylori. These data provide confirmatory evidence for the importance of those proteins in the development of H. pylori-associated gastric disorders.

Antimutagenic Effect of Kimchi

Rhee, Sook Hee,Park, Kun Young,Baek, Kuung A,Cheigh, Hong Sik 부산대학교 김치연구소 1995 김치의 과학과 기술 Vol.1 No.-

The methanol extract of kimchi (control and anchovy added' baechu kimchi) showed antimutagenic activities toward a0atoxin B_1 (AFB_1), and 4-nitroquinoline-l-oxide (4-NQO) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in the Ames test and SOS chromotest, respectively. The kimchi samples were fermented at 5℃ for 0, 3 and 6 weeks. The 3-week-fermented kimchi was optimally ripened with the pH of 4.4∼4.5, while 6-week-fermented kimchi was overripened with the pH of 3.5∼4.0. The mutagenicities mediated by AFB_1 reduced by 35∼75% when the methanol extracts of control or anchovy added kimchi added at the levels of 1% and 5% in the Ames test. The 3-week-fermented control kimchi showed the highest inhibition rate of 75% when 5% of the sample was added. The antimutagenic effects of the kimchi samples also observed in MNNG and 4-NQO on SOS chromotest with the same fashions as shown in the AFB,. The inhibition rates of the mutagenicities were the highest at 3 week-fermented samples, and when the adding concentration increased the effect increased. There was no difference on the activities of the antimutagenicity between the control and anchovy added kimchi. The active compound(s) was found in the chloroform fraction from the methanol extract of the kimchi.

전산유체해석을 이용한 다양한 요철 형상에 대한 고압터빈 노즐 냉각유로 최적화 및 냉각 성능 비교

이상아(S. A. Lee),이동호(D. H. Rhee),강영석(Y. S. Kang),이관중(K. J. Yee),김규홍(K. H. Kim) 한국전산유체공학회 2014 한국전산유체공학회지 Vol.19 No.4

This study conducts shape optimization of rib turbulator on the internal cooling passage that has triangular cross-section of high pressure turbine nozzle. During optimization, various types of rib turbulator including angled, V-shaped, A-shaped and angled rib with intersecting rib are considered. Each type of rib turbulator is parameterized with attack angle(s), rib height, spacing ratio and bending/intersecting location. For optimization, Design of Experiment (DOE) and Kriging surrogate model are used to utilize computational resource more efficiently and Genetic Algorithm (GA) is used to search the optimum points. As a result, Pareto front of each type of rib turbulator with friction factor that relates to pressure drop in cooling passage and spatially averaged Nusselt number that relates to heat transfer on the wall is drawn and optimum points on the Pareto front are suggested.

Spinach Saponin-Enriched Fraction Inhibits Platelet Aggregation in cAMP- and cGMP-Dependent Manner by Decreasing TXA2 Production and Blood Coagulation

( Hyun Jeong Cho ),( Sun A Choi ),( Chun Gyu Kim ),( Tae Sung Jung ),( Jeong Hwa Hong ),( Man Hee Rhee ),( Hye Jin Park ),( Hwa Jin Park ) 한국응용약물학회 2011 Biomolecules & Therapeutics(구 응용약물학회지) Vol.19 No.2

In this study, we investigated the effect of spinach saponin-enriched fraction (SSEF) on collagen (10 μg/ml)-stimulated platelet aggregation. SSEF inhibited collagen-induced platelet aggregation, and which was involved in the inhibition of thromboxane A2 (TXA2) production, an intracellular Ca2+ -agonist as an aggregation-inducing autacoidal molecule. In addition, SSEF significantly increased the formation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), intracellular Ca2+ -antagonists as aggregation-inhibiting molecules, in collagen-stimulated platelets. These results suggest that SSEF might inhibit Ca2+-elevation and TXA2 formation by increasing the production of Ca2+-antagonistic molecules cAMP and cGMP. These mean that SSEF is a potent inhibitor of collagen-stimulated platelet aggregation. On the other hand, prothrombin time (PT) and activated partial thromboplastin time (APTT) were potently prolonged by SSEF. These findings suggest that SSEF prolongs the internal time between the conversion of fibrinogen to fibrin. Accordingly, our data demonstrate that SSEF may be a crucial tool for a negative regulator during platelet activation and blood coagulation on thrombotic diseases.

Inhibitory Effects of Panaxatriol from Panax ginseng C. A. Meyer on Phosphoinositide Breakdown Induced by Thrombin in Platelets

Park, Kyeong-Mee,Rhee, Man-Hee,Shin, Han-Jae,Song, Yong-Bum,Hyun, Hak-Chul,Park, Ki-Hyun,Cho, Hyun-Jeong,Choi, Sun-A,Kang, Hyo-Chan,Kim, Kyoung-Jin,Kim, Hyeong-Soo,Kang, Hee-Jin,Ok, Woo-Jeong,Lee, Don The Korean Society of Ginseng 2008 Journal of Ginseng Research Vol.32 No.2

In this study, we have investigated the effect of panaxatriol (PT) on phosphoinositides (PIS) breakdown and $Ca^{2+}$-elevation in thrombin-induced platelet aggregation. Thrombin (5U/ml), a potent platelet agonist which activates phospholipase $C_{\beta}$ via protease activated receptor (PAR), hydrolyzed PIS in platelet membrane. The phosphatidylinositol 4, 5-bisphosphate $(PIP_2)$ was hydrolyzed after 10 sec of the thrombin-stimulation, and both the phosphatidylinositol 4-monophosphate (PIP) and phosphatidylinositol (PI) were brokendown after 30 sec of the thrombin-stimulation. However, PT inhibited the thrombin-stimulated hydrolysis of $PIP_2$, PIP, and PI. On the other hand, thrombin increased the level of phosphatidic acid (PA) which is phosphorylated from diacylglycerol (DG) generated by PIS-hydrolysis. However, Pr inhibited the thrombin-increased PA level non-significantly. Thrombin increased cytosolic free $Ca^{2+}([Ca^{2+}])_i$) up to 72% as compared with control $(30.8{\pm}0.9 nM)$ in intact platelet. However, PT (100 ${\mu}g/ml$) inhibited the thrombin-elevated $[Ca^{2+}]_i$ to 100%. These results suggest that PT may have a beneficial effect on platelet aggregation-mediated thrombotic disease by inhibiting thrombin-induced platelet aggregation via suppression of the $[Ca^{2+}]_i$ level and PIS breakdown.

Inhibitory Effects of Panaxatriol from Panax ginseng C. A. Meyer on Phosphoinositide Breakdown Induced by Thrombin in Platelets

Kyeong-Mee Park,Man-Hee Rhee,Han-Jae Shin,Yong-Bum Song,Hak-Chul Hyun,Ki-Hyun Park,Hyun-Jeong Cho,Sun-A Choi,Hyo-Chan Kang,Kyoung Jin Kim,Hyeong-Soo Kim,Hee-Jin Kang,Woo-Jeong Ok,Dong-Ha Lee,Hwa-Jin P 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.2

In this study, we have investigated the effect of panaxatriol (PT) on phosphoinositides (PIS) breakdown and Ca<SUP>2+</SUP>-elevation in thrombin-induced platelet aggregation. Thrombin (5U/ml), a potent platelet agonist which activates phospholipase Cβ via protease activated receptor (PAR), hydrolyzed PIS in platelet membrane. The phosphatidylinositol 4, 5-bisphosphate (PIP₂) was hydrolyzed after 10 sec of the thrombin-stimulation, and both the phosphatidylinositol 4-monophosphate (PIP) and phosphatidylinositol (PI) were brokendown after 30 sec of the thrombin-stimulation. However, PT inhibited the thrombin-stimulated hydrolysis of PIP₂, PIP, and PI. On the other hand, thrombin increased the level of phosphatidic acid (PA) which is phosphorylated from diacylglycerol (DG) generated by PIS-hydrolysis. However, PT inhibited the thrombin-increased PA level non-significantly. Thrombin increased cytosolic free Ca<SUP>2+</SUP>[Ca<SUP>2+</SUP>]i) up to 72% as compared with control (30.8±0.9 nM) in intact platelet. However, PT (100 ㎍/ml) inhibited the thrombin-elevated [Ca<SUP>2+</SUP>]i to 100%. These results suggest that PT may have a beneficial effect on platelet aggregation-mediated thrombotic disease by inhibiting thrombin-induced platelet aggregation via suppression of the [Ca<SUP>2+</SUP>]i level and PIS breakdown.

국산 아피오스(Apios americana Medikus) 감자의 식품학적 성분분석

김영현 ( Y. H. Kim ),이성갑 ( S. K. Rhee ),이애리 ( A. R. Lee ),김단비 ( D. B. Kim ),이옥환 ( O. H. Lee ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2014 강원 농업생명환경연구 Vol.26 No.1

본 연구에서는 국내산 아피오스 감자의 식품학적 성분을 분석하고자 동결건조 된 시료의 일반성분, 아밀로오스, 식이섬유, 비타민 B2, 비타민 C 및 색도를 분석하였다. 분석결과, 아피오스 감자의 조단백질, 조지방, 조회분및 탄수화물 함량은 각각 13.79%, 3.03%, 5.10% 및 78.08%를 차지하고 있는 것으로 나타났다. 아피오스 감자의 아밀로오스 함량은 9.34%이었고, 식이섬유 함량은 20.38%로 나타났다. 아피오스 감자의 비타민 B2 및 비타민 C의 함량은 각각 0.07 mg/100 g, 74 mg/100 g이었으며, Hunter 색체계를 이용하여 측정한 아피오스 감자의 명도(L값), 적색도(a값), 황색도(b값)는 각각 84.08 ± 0.01, 0.10± 0.02, 5.19 ± 0.01이었다. 본 연구는 아피오스 감자의 식품성분을 분석한 것으로 아피오스 감자의 식품가공 및 건강기능식품 이용을 위한 기초자료로 활용이 가능한 것으로 판단된다. This study was to investigate the food components of apios pota b values of apios potato were 84.08 ± 0.01, 0.10 ± 0.02, 5.19 ± 0.01, respectively. The contents of vitamin B2 and vitamin C were 0.07 mg and 74 mg, respectively. In addition, amylose and dietary fiber contents were 9.34 and 20.38% respectively. These result indicate that apios potato has a rich dietary fiber and nutritional components as a food ingredient and functional food materials.

진공증착박막 MnAl의 자기적 성질에 관한 연구

李章魯,우안진,장현숙 숙명여자대학교 자연과학연구소 1989 자연과학논문집 Vol.- No.1

Magnetic and other properties of MnAl films prepared by vacuum evaporation were examined. Mixed powders of Mn and Al were used as an evaporation source. To find the optimum conditions of fabrication of MnAl evaporated film, the dependence on substrate temperature and the concentration of evaporation source was investigated. The optimum conditions of fabrication of films were found to be as follows, substrate temperature : 400℃, concentration of Mn: 72∼74 wt %. Magnetic properties of the films evaporated under these condition were 550∼554 Oe in coercivity, 0.62 in squareness, 2.14∼2.90×10³ emu/cm³ in saturation induction and 410℃ in the Curie temperature. The easy axis of magnetization was normal to the coated film surface. In order to increase the saturation induction, element cu was added. Addition of Cu has an effect on increasing of the saturation induction at the sacrifice of deterioration of the coercivity.

Foxa2 and Nurr1 Synergistically Yield A9 Nigral Dopamine Neurons Exhibiting Improved Differentiation, Function, and Cell Survival

Lee, H. S.,Bae, E. J.,Yi, S. H.,Shim, J. W.,Jo, A. Y.,Kang, J. S.,Yoon, E. H.,Rhee, Y. H.,Park, C. H.,Koh, H. C. ALPHAMED PRESS INC 2010 Stem Cells Vol.28 No.3

Avoidance of Internal Resonances in Hemispherical Resonator Assemblies from Fused Quartz Connected by Indium Solder

Sergii A. Sarapuloff(세르게이 사라플로프),Huinam Rhee(이희남),Sang-Jin Par(박상진) 한국소음진동공학회 2013 한국소음진동공학회 학술대회논문집 Vol.2013 No.4

Modern solid-state gyroscopes (HRG) with hemispherical resonators from high-purity quartz glass and special surface superfinishing and ultrathin gold coating become the best instruments for precise-grade inertial reference units (IRU) targeting long-term space missions. Designing of these sensors could be a notable contribution into development of Korea as a space nation. In participial, 40mm diameter thin-shell resonator from high-purity fused quartz, fabricated as a single-piece with its supporting stem has been designed, machined, etched, tuned, tested, and delivered by STM Co. (ATS of Ukraine) several years ago; an extremely-high Q-factor (upto 10~20 millions) has been shown. Understanding of the best way how to match such a unique sensor with inner glass assembly of the gyro means how to use the high potential in a maximal extent; and this has become the urgent task. Inner quartz glass assembly has a very thin indium (In) layer soldered the resonator and its silica base (case), but effects of internal resonances between operational modal pair of the shell-cup and its side (parasitic) modes can notable degrade the potential of the sensor as a whole, instead of so low level of resonator’s intrinsic losses. Unfortunately, there are special combinations of dimensions of the parts (so-called, “resonant sizes”), when intensive losses of energy occurs. The authors proposed to use the length of stem’s fixture as an additional design parameter to avoid such cases. So-called, a cyclic scheme of finite element method (FEM) and ANSYS software were employed to estimate different combinations of gyro assembly parameters. This variant has no mismatches of numerical origin due to FEM’s discrete mesh. The optimum length and dangerous “resonant lengths” have been found. The special attention has been paid to analyses of 3D effects in a cup-stem transient zone, including determination of a difference between the positions of geometrical Pole of the resonant hemisphere and of its “dynamical Pole”, i.e., its real zone of oscillation node. Boundary effects between the shell (cup) and 3D short “beams” (inner and outer stems) have been ranged. The results of the numerical experiments have been compared with the classic model of a quasi-hemispherical shell band with inextensional midsurface, and the solution using Rayleigh’s functions of the 1<SUP>st</SUP> and 2<SUP>nd</SUP> kinds. To guarantee the truth of the recommended sizes to a designer of the real device, the analytical and FEM results have been compared with experimental data for a party of real resonators. The consistency of the results obtained by different means has been shown with errors less than 5%. The results notably differ from the data published earlier by different researchers.