무지개송어(Onchorhynchus mykiss) 위팽창증후군의 잠재적 원인체의 분자유전학적 동정

노형진,김도형,Roh, Heyong Jin,Kim, Do-Hyung 한국수산과학회 2017 한국수산과학회지 Vol.50 No.5

A rainbow trout Onchorhynchus mykiss farm located in Gangwon province, South Korea, experienced approximately 10% mortality in June 2017. Most diseased fish had a markedly distended, gas-filled stomach, and exhibited abnormal behavior at the water surface. In this study, we attempted to identify the cause of stomach distension syndrome in those rainbow trout. The stomach of most of the affected fish were full of unidentified gases and some exudate, and yeast was isolated from the stomach mucosa. Pure cultures of yeast were identified using a multilocus sequence typing scheme based on 18S rRNA, internal transcribed spacers, large subunit rRNA, and the gene encoding the largest subunit of RNA polymerase II (RPB1). The RPB1 gene sequences were compared with those of related species available in a database. The yeast was identified as Scheffersomyces coipomoensis (Candida coipomoensis) based on sequence analyses. This is the first study to reveal that Sch. coipomoensis is a potential causative agent of stomach distension syndrome in farmed rainbow trout. Our results will be helpful for future related studies, and indicate that farmers and stakeholders should observe this emerging disease closely.

제주도 연안 해역의 오염지표세균의 분포

노형진 ( Heyong Jin Roh ),임윤진 ( Yun-jin Lim ),김아란 ( Ahran Kim ),김남은 ( Nam Eun Kim ),김영재 ( Youngjae Kim ),박노백 ( Noh Back Park ),황지연 ( Jee-youn Hwang ),권문경 ( Mun-gyeong Kwon ),김도형 ( Do-hyung Kim ) 한국수산과학회(구 한국수산학회) 2018 한국수산과학회지 Vol.51 No.4

We examined correlations of the density of fish farms with the distributions of indicator bacteria (Escherichia coli, fecal streptococci) and a bacterial fish pathogen (Streptococcus parauberis) off the coastline of Jeju Island. Seawater samples were collected at four coastal sites on the Island [Aewol (control), Gujwa, Pyoseon and Daejeong] in June, August and October 2016. The indicator bacteria were generally more frequently isolated from samples taken in August when water temperatures and human activities on nearby beaches were highest. Although fish farms were least common at Daejeong, the numbers of isolated fecal indicator bacteria were highest in the seawater and effluent water collected from this site. Hence, fish farms were not likely major contributors of indicator bacteria at Daejeong. We found discrepancies between the isolated bacterial counts and the predicted bacterial copy numbers deduced from our qPCR results, indicating that this pathogen may exist in a viable but non-culturable (VBNC) state in seawater. Thus, livestock wastewater and chemical fertilizer loading off Jeju Island may negatively impact seawater quality more than the effluent released from fish farms does.

토너 바인더용 분지화된 탄성 폴리에스테르 공중합체의 합성

노형진(Hyung Jin Roh),임종관(Jong Kwan Lim),이동호(Dong Ho Lee),윤근병(Keun Byoung Yoon) 한국고분자학회 2012 폴리머 Vol.36 No.4

분지화된 폴리에스테르계 공중합체를 제조하고, 공중합체의 분자량, 용융점도, T g, 1/2 method temperature(T 1/2) 및 레올로지 특성을 고찰하여 레이저 프린터 토너 바인더로의 적용을 검토하였다. 디메틸테레프탈레이트(DMT), 에 틸렌글리콜(EG), 2,2-bis(4-(2-hydroxypropoxy)phenyl)propane(HPP)으로 제조한 선형공중합체는 분자량이 낮고, 용융 탄성이 낮아 이를 개선하기 위하여 분지제로 3 또는 4 관능기를 가진 2-(hydroxymethyl)-2-ethylpropane-1,3-diol (trimethylol propane, TMP), 2,2-bi(hydroxymethyl)-1,3-propanediol(pentaerythritol, PER), 1,2,4-benzenetricarboxylic anhydride(trimellitic anhydride, TMA), 글리세롤 등을 첨가하여 분지화된 공중합체를 제조하였다. 분지제의 함량과 종류에 따른 열적, 레올로지 특성을 고찰하였다. 분지제의 함량이 15 mol% 이상 첨가한 공중합체는 140 oC 정도의 T 1/2 값을 가지고, 높은 분자량과 용융탄성을 나타내어 토너를 융착 인쇄하는 레이저 프린터의 핫멜트 토너로서 적당 하였다. The branched copolyester was synthesized and its molecular weight, Tg, 1/2 method temperature (T1/2) and rheological properties were characterized for the application of toner binder. The linear copolyester had low molecular weight and melt elasticity obtained by dimethylterephthalate (DMT), ethylene glycol (EG) and 2,2-bis(4-(2-hydroxypropoxy) phenyl)propane (HPP). The branched copolyesters prepared with various branching agents such as 2- (hydroxymethyl)-2-ethylpropane-1, 3-diol (trimethylol propane, TMP), 2, 2-bi (hydroxymethyl)-1, 3-propanediol (pentaerythritol, PER), 1, 2, 4-benzenetricarboxylic anhydride (trimellitic anhydride, TMA) and glycerol to improve the physical properties of the linear copolyester. The effect of branching agents on the molecular weight and melt elasticity of the branched copolyester was examined. The branched copolyesters prepared by adding over 15 mol% of branching agent showed relatively high molecular weight and melt elasticity, and T1/2 value of 140˚C. Therefore, the highly branched copolyesters were deemed suitable as a hot-melt toner of laser print process.

양식 넙치에서 Parvicapsula anisocaudata의 감염 사례에 대한 보고

김남은,김아란,노형진,강경식,김도형,Kim, Nam Eun,Kim, Ahran,Roh, Heyong Jin,Gang, Kyoung Sik,Kim, Do-Hyung 한국어병학회 2018 한국어병학회지 Vol.31 No.2

Parvicapsula anisocaudata, a myxosporean parasite, is presumably one of causative agents of emaciation in olive flounder Paralichthys olivaceus in Korea. In this study, we report a case of unusual abdominal distension due to exceptionally enlarged liver in farmed olive flounder. For the identification of the causative agent, bacteria and nucleic acids of virus that are possibly present were attempted to isolate from internal organs of five fish sampled from a fish farm in Jeju. Although a few bacterial colonies were isolated from some samples, there was no evidence that fish were primarily affected by virus and/or bacteria. From histopathological analysis, myxosporean were found in almost all internal organs, particularly in the stomach. The causative agent was identified as P. anisocaudata by sequencing a part of small subunit rRNA. This study contains a very unusual case of olive flounder heavily and systemically infected with P. anisocaudata, showing excessively enlarged liver with a small amount of ascitic fluid.

Measurement of Total Plasma Homocysteine in Patients with Chronic Renal Failure Using HPLC/FLD

Kyung-Ok Lee(이경옥),Bo-Kyung Kang(강보경),Hyung-Jin Roh(노형진),Kwang-Suk Ryoo(류광석),Jeong-Yeon Yoo(유정연),Man-Jeong Paik(백만정),Kang-Hyeob Lee(이강협) 대한의생명과학회 1997 Biomedical Science Letters Vol.3 No.1

본 연구에서는 한국인 만성신부전증 환자에서 심혈관계 질환의 조기진단을 위한 생화학적 표지자로서 homocysteine의 임상적 유용성을 검토하기 위하여, 만성신부전증 환자와 건강인에서 HPLC/FLD (high performance liquid chromatography/fluorescence detector)를 이용하여 혈장 homocysteine 농도를 측정하고 그 결과를 비교하였다. 본 실험방법의 회수율은 98.6±5.8%를 나타내었으며, 0.2 n㏖/L보다 낮은 농도까지 측정이 가능하였고, 2~50 n㏖/㎖까지 직선성이 성립하였다 (correlation coefficient =0.9997). 한국인 중 건강한 정상인 (20명)과 만성신부전증 환자 (90명)에서 혈장 homocysteine은 각각 6.81±1.54 n㏖/㎖과 27.28±14.94n ㏖/㎖이었으며, 환자군에서는 정상인군에 비하여 약 4 배 정도 높은 수치를 나타내었다 (p<0.05). 본 실험의 결과로 볼 때 HPLC/FLD를 이용한 homocysteine 측정은 예민도와 재현성이 높아 routine 실험실 방법으로 유용성이 높을 것으로 생각되며, 또한 혈장 homocysteine의 측정은 만성신부전증 환자의 주요한 사망원인이 되는 폐쇄성 동맥질환을 조기에 진단하거나, 혹은 homocysteine 농도를 저하시키는 치료를 실시한 후 치료효과를 판단하기 위한 생화학적 marker로 활용될 수 있을 것으로 기대된다. Cardiovascular disease has been the leading cause of death among patients with chronic renal failure. Many reports have been described that homocysteine is one of the independent risk factor to the occulsive vascular disease. In this study, HPLC/FLD (high performance liquid chromatography-fluorescence detector) technique was used to measure homocysteine level in patients with chronic renal failure and normal control group. The detection limit and recovery of total plasma homocysteine using HPLC/FLD were 98.6±5.8% and 0.2 n㏖/㎖, respectively. The linearity of this method was established in concentration range of 2~50 n㏖/㎖ (correlation coefficient=0.9997). The concentrations of total plasma homocysteine were 6.81 ± 1.54 n㏖/㎖ and 27.28 ± 14.94 n㏖/㎖ in normal control (n=20) and patient group (n=90), respectively (p<0.05). In this study, the HPLC/FLD method showed high sensitivity and reproducibility for a routine clinical laboratory testing. Moreover determination of homocysteine level in plasma might be useful for a biochemical marker for predicting the cardiovascular diseases and for monitoring of therapeutic effect of lowering homocysteine in patients with chronic renal failure.

주사슬에 말로네이트기를 가지는 신규 폴리에스테르의 합성과 광분해 특성을 이용한 형광 이미지 패터닝

정선주(Seon Ju Jeong),곽기섭(Gi Seop Kwak),정인태(In Tae Jung),이동호(Dong Ho Lee),노형진(Hyung Jin Roh),윤근병(Keun Byoung Yoon) 한국고분자학회 2008 폴리머 Vol.32 No.1

日本式 생산시스템의 特長과 限界

노형진 京畿大學校 韓國産業經濟硏究所 1994 産業硏究 Vol.5 No.-

Measurement of Total Plasma Homocysteine in Patients with chronic Renal Failure Using HPLC/FLD

Lee,Kyung-Ok,Yoo,Jeong-Yeon,Roh,Hyung-Jin,Paik,Man-Jeong,Lee,Kang-Hyeob,Kang,Bo-Kyung,Ryoo,Kwang-Suk THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1997 Journal of biomedical laboratory sciences Vol.3 No.1

본 연구에서는 한국인 만성신부전증 환자에서 심혈관계 질환의 조기진단을 위한 생화학적 표지자로서 homocysteine의 임상적 유용성을 검토하기 위하여, 만성신부전증 환자와 건강인에서 HPLC/FLD (high performance liquid chromatography/fluorescence detector)을 이용하여 혈장 homocysteine 농도를 측정하고 그 결과를 비교하였다. 본 실험방법의 회수율은 98.6±5.8%를 나타내었으며, 0.2 nmol/L보다 낮은 농도까지 측정이 가능하였고, 2∼50 nmol/ml까지 직선성이 성립하였다 (correlation coefficient=0.9997). 한국인 중 건강한 정상인 (20명)과 만성신부전증 환자 (90명)에서 혈장 homocysteine은 각각 6.81±1.54 nmol/ml과 27.28±14.94 nmol/ml이었으며, 환자군에서는 정상인군에 비하여 약 4배 정도 높은 수치를 나타내었다 (p<0.05). 본 실험의 결과로 볼 때 HPLC/FLD를 이용한 homocysteine 측정은 예민도와 재현성이 높아 routine 실험실 방법으로 유용성이 높을 것으로 생각되며, 또한 혈장 homocysteine의 측정은 만성신부전증 환자의 주요한 사망원인이 되는 폐쇄성 동맥질환을 조기에 진단하거나, 혹은 homocysteine농도를 저하시키는 치료를 실시한 후 치료효과를 판단하기 위한 생화학적 marker로 활용될 수 있을 것으로 기대된다. Cardiovascular disease has been the leading cause of death among patients with chronic renal failure. Many reports have been described that homocysteine is one of the independent risk factor to the occulsive vascular disease. In this study, HPLC/FLD (high performance liquid chromatography-fluorescence detector) technique was used to measure homocysteine level in patients with chronic renal failure and normal control group. The detection limit and recovery of total plasma homocysteine using HPLC/FLD were 98.6±5.8% and 0.2 nmol/ml, respectively. The linearity of this method was established in concentration range of 2∼50 nmol/ml, (correlation coefficient=0.9997). The concentrations of total plasma homocysteine were 6.81±1.54 nmol/ml and 27.28±14.94 nmol/ml in normal control (n=20) and patient group (n=90), respectively (p<0.05). In this study, the HPLC/FLD method showed high sensitivity and reproducibility for a routine clinical laboratory testing. Moreover determination of homocysteine level in plasma might be useful for a biochemical marker for predicting the cardiovascular diseases and for monitoring of therapeutic effect of lowering homocysteine in patients with chronic renal failure.