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IMS-RT-PCR을 활용한 육성돈 분변으로부터 간염 A형 바이러스의 신속순수분리 및 검출법 연구
이희민(Hee-Min Lee),김두운(Duwoon Kim) 한국식품과학회 2012 한국식품과학회지 Vol.44 No.5
Human pathogenic viruses such as hepatitis A and E virus (HAV and HEV), which lead to acute liver failure and death, are foodborne pathogens associated with the consumption of virus-contaminated meats, filter-feeding bivalves, fruits, and salads. Two of the three swine farms examined in this study had HAV and HEV positive stool samples in a nested RT-PCR assay. The use of the immunomagnetic separation (IMS) facilitated the separation of HAV through interactions between the ligand on the virion surface and the antibody from the swine feces containing both HAV and HEV. The nested RT-PCR analysis was performed for the detection of HAV obtained from hepatocarcinoma cell line (PLC/PRF/5) contaminated with eluent fraction of IMS. This indicated that IMS has the potential to simultaneously isolate and concentrate target viruses by changing antibodies linked on the magnetic beads.
레스토랑 고객성격이 감정과 행동의도에 미치는 영향에 관한 연구
이희민(Lee, Hee-min),남궁영(Namkung, Young) 한국외식경영학회 2018 외식경영연구 Vol.21 No.5
본 연구의 목적은 첫째, 고객 성격이 레스토랑의 식사 중에 고객이 경험하는 감정에 미치는 영향력을 검증하며, 둘째, 고객 성격이 행동의도에 미치는 영향력을 검증하며, 셋째, 고객이 경험한 감정이 행동의도에 미치는 영향력을 검증하고자 하였다. 본 연구에서는 최근 3개월 이내에 테이블 서비스 레스토랑을 방문한 소비 자를 대상으로 조사를 하였으며, 유효 표본은 388개가 사용되었다. 연구 결과는 다음과 같다. 첫째, 요인분석 결과 고객 성격은 5개로 분류하였으며, 외향성, 우호 성, 성실성, 신경증, 개방성으로 구분하였다. 둘째, 회귀분석 결과 고객 성격의 5 가지 유형 중 외향성, 우호성, 개방성은 긍정적 감정에 정의(+)영향을 미치며, 신경증, 개방성은 부정적 감정에 각각 정(+)의 영향과 부(-)의 영향을 미치는 것으로 나타났다. 셋째, 고객 성격 유형 5가지 유형 중 외향성, 우호성, 개방성은 행동 의도에 정(+)의 영향을 미치는 것으로 나타났다. 넷째, 고객이 경험한 긍정적 감정은 행동의도에 정(+)의 영향력을 미치는 것으로 나타났고, 부정적 감정은 행동 의도에 부(-)의 영향력을 미치는 것으로 나타났다. 이러한 연구 결과를 바탕으로 레스토랑 경영자 및 실무자를 위하여 이론, 실무적 마케팅 시사점을 제시하였다. The purpose of this study is to 1) examine the effect of customer personality on the emotions experienced by the customers during restaurant dining, 2) investigate the effect of customer personality on behavior intention, and 3) assess the effect of emotions on behavioral intention. A total of 388 samples who had visited a table service restaurant within the last three months participated in this study. The results of the study are as follows. First, factor analysis identified five customer personality such as extroversion, agreeableness, conscientiousness, neuroticism, and openness. Second, regression analysis showed that extroversion, agreeableness, and openness had positive(+) significant effects on positive emotions, while neuroticism showed a positive(+) significant effect on negative emotion, and openness has a negative(-) significant effect on negative emotion. Third, extraversion, agreeableness, and openness among customer personality types had positive(+) effects on behavioral intention. Fourth, positive emotions experienced by customers had a positive(+) effect on behavioral intention while negative emotion had a negative(-) effect on behavioral intention. Based on these results, theoretical and practical implications for effective marketing for restaurant practitioners and operator were suggested.
Nitroprusside가 인간정자의 생존력, 운동성, Reactive Oxygen Species 발생에 미치는 영향
민부기,이희민,김기석,이희섭,김흥곤,홍기연,이봉주,Min, Bu-Kie,Lee, Hee-Min,Kim, Ki-Seok,Lee, Hee-Sup,Kim, Heung-Gon,Hong, Gi-Youn,Lee, Bong-Ju 대한생식의학회 1996 Clinical and Experimental Reproductive Medicine Vol.23 No.3
Objective: To analyze the direct effect of nitre oxide, generated from sodium nitroprusside, on sperm motility and reactive oxygen species. Design: Human sperm samples were treated to allow swim-up and washing. And the samples were devided into four aliquots. Each aliquot was incubated with either concentration at 0, 100nM, $10{\mu}M$, 1mM of nitroprusside. Intervention: Samples were measured chemiluminosence for reactive oxygen species of each aliquot with concentrations at 0, 100nM, $10{\mu}M$, 1mM of nitroprusside at allowing swim-up and washing of sperm. Main Outcome Measures: Percent motion parameters and viability were asse-ssed at 0, 3, 6, 12, 24 hours incubation. Results: The percent viablity was lower slightly in control group (50.2%) than that in sperm treated with 100nM of nitroprusside(57.5%) at 24 hours after incubation, while was reduced significantly in sperm with concentra-tion of $10{\mu}M(42.1%)$ and 1mM(21.3%)of nitroprusside at 6 hours after incubation. And the sperm treated with 1mM of nitroprusside was immotile totally at 6 hours after incubation. The straight line$(35.3{\pm}5.6%)$, the rapid forward$(37.2{\pm}6.4%)$ and the weak curvilinear velocity$(9.6{\pm}2.4%)$were more favorable comparing with those ($32.4{\pm}4.2%$, $30.0{\pm}7.8%$ and $18.0{\pm}4.6%$ respectively) in control group at 3 hours after incubation, but reduced significantly in sperm treated with $10{\mu}M$ and 1mM of nitroprusside. The levels of reactive oxygen species in control(700 c.p.m.) is lower significantly than that in each experimental groups of sperm treated with nitroprusside. And the levels of reactive oxygen species were 2200 c.p.m. in 100nM, 6200c.p.m. in $1{\mu}M$ and 12800c.p.m. in 1mM respectively. Conclusion: These results suggested that the concentration of 100nM of nitroprusside on sperm is beneficial to the maintanance of viablity and motile velocity, but detriment in high concentration of $10{\mu}M$ or 1mM of nitroprusside.
프로필렌 카보네이트로 가소화시킨 폴리(L-락트산) 필름의 열적 및 기계적 특성
이강국(Ganggook Lee),이희민(Hee Min Lee),김영호(Young Ho Kim) 한국고분자학회 2019 폴리머 Vol.43 No.1
폴리(L-락트산)(PLA)에 프로필렌 카보네이트(PC)를 첨가하여 PLA의 낮은 파단신도와 충격강도를 개선하면서 PC가 PLA의 가소제로 작용하는지에 대한 연구를 진행하였다. 이를 위해 여러 함량의 PC가 포함된 PLA/PC 필름을 용융압착법으로 제조한 후 열중량분석기, 시차주사열량분석기, 동적기계적분석기로 열적 특성을, 만능시험기 및 Charpy 충격시험기로 기계적 물성을, 광각 X-선과 주사전자현미경으로 결정형태와 모폴로지를 분석하였다. 실험결과, PLA/PC 필름에 PC 함량이 많아짐에 따라 유리전이온도, 냉결정화온도, 용융온도가 모두 낮아지면서 결정화도가 감소하였지만, PLA 결정형태는 일정하였다. 또한 PLA/PC 필름의 항복응력, 초기탄성률 및 파단응력은 감소하였지만 파단변형률과 충격강도는 크게 증가하였다. 이러한 모든 결과들이 PC가 PLA의 가소제로 작용한다는 것을 나타내었다. Propylene carbonate (PC) was added to poly(L-lactic acid) (PLA) film in order to improve both low elongation-at-break and low impact strength, and the plasticization effect of PC on PLA was examined. PLA films containing various amounts of PC were prepared by melt pressing after melt pre-mixing, and their thermal properties were analyzed with a thermogravimetric analyzer, differential scanning calorimeter, and dynamic mechanical analyzer; mechanical properties with a universal testing machine and charpy impact testing machine; crystallinity and morphology with a wide angle X-ray diffractometer and scanning electron microscope. As the PC content in PLA/PC film increased, glass transition temperature, cold crystallization temperature, and melting temperature of PLA shifted to lower temperature and the crystallinity decreased, while the crystal form was unchanged. The yield stress, initial modulus, and tensile stress-at-break of the films decreased but the tensile strain-at-break and impact strength increased dramatically with increasing PC content. All the results indicated that PC acts as a plasticizer for PLA.
건축물 해체공사의 통합 안전관리 가이드라인 개발 및 적용성 평가
최종수,허성태,고원준,이희민,Choi, Jong-Soo,Heo, Seong-Tae,Go, Won-Joon,Lee, Hee-Min 한국건설관리학회 2010 한국건설관리학회 논문집 Vol.11 No.6
Safety management for building deconstruction works has been received extremely limited attention in the domestic construction society. Lack of systematic safety management practices and defective education system lead to the high level of fatality compared to that of advanced countries. Under the above domestic deconstruction environment, the authors intend to develope an integrated safety management guidelines that covers both mechanical and partial deconstruction works. Research outcome includes such specific educational materials as safety manual, checklists, pocket pamphlets, and e-manuals. Furthermore, to validate the applicability of the above materials, questionnaire survey and interviews were conducted. Detailed descriptions are provided for each research outcome. 국내 건축물 해체공사의 안전관리 분야는 연구, 제도정비 및 실무 차원에서 소외되어온 경향이 있다. 해체공사의 수행과정에 있어서 체계적이지 못한 안전관리 실태, 부실한 교육체계, 활용할 수 있는 교육용 교재의 미흡 등은 안전사고의 빈도 및 사고발생시 중대사고로 이어지는 주요 원인이 되며 그 수준은 외국의 경우와 비교하여 볼 때 대단히 높은 실정이다. 본 연구에서는 이러한 국내 해체업계 현황의 개선을 목표로 기계식 전면해체공사 및 리모델링을 위한 부분해체공사를 포괄하는 통합 안전관리 가이드라인을 개발하였다. 구체적인 연구 성과물은 안전관리매뉴얼, 체크리스트, 포켓용 팸플릿, 전자매뉴얼 등이며, 연구성과의 검증을 위하여 해체공사 유경험자를 대상으로 실무에 대한 적용성 평가를 수행하였다.