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TBTCl 노출에 의한 대복, Gomphina veneriformis (Bivalvia: Veneridae) 외투막의 미세구조적 변화
박정준,김석렬,박명애,이정식,Park, Jung-Jun,Kim, Seok-Ryel,Park, Myoung-Ae,Lee, Jung-Sick 한국패류학회 2011 The Korean Journal of Malacology Vol.27 No.3
Equilateral venus, Gomphina veneriformis exposed to tribultyltin chloride (TBTCl) for 36 weeks was showed ultrastructural changes of the mantle. The fine mantle had 4-folds and its epidermal layer consisted of simple columnar epithelial cells and ciliated cells and secretory cells. Inner and outer epidermal layer covered connective tissue. The mantle exposed to TBTCl at 12 weeks was decreased cilia in the inner epidermal layer, and observed extension of the hemolymph sinus and destruction of the septum. At 20 weeks, it revealed vacuole formation and pycnosis in the cytoplasm, and scattered muscular fiber. After 28 weeks of exposure, the mantle revealed partially degenerative changes in the epidermal layer. In the ciliated cells, basal body was isolated from the cilia and rootlet complex and basal foot were scattered. The sarcolemma had debris fiber. At 36 weeks, it observed degenerative cells that it revealed disappearance of the cilia, atrophic nucleus, poorly membrane and destruction of the cresternae in the mitochondria, and increasing heterophagosome. The outer epithelial cell had necrotic nuclus, numeous lysosome and disappearance of the microvilli. Therefore, results of this study suggested that chronical TBTCl exposure in the Gomphina veneriformis induced the disorders of shell growth and physiological function with histopathological changes of the mantle. TBTCl에 36주 동안 노출된 대복, Gomphina veneriformis 의 미세구조적인 변화를 전자현미경으로 관찰하였다. 대복의 외투막은 가장자리에 4개의 주름을 가지며, 내부와 외부상피층이 단층의 원주형 상피세포, 섬모세포, 분비세포로 구성되어 있었다. 이들 상피층은 혈림프동이 존재하는 결합조직층을 둘러싸고 있었다. 대복의 외투막은 TBTCl 노출 12주째 내부상피층에 존재하는 섬모의 수가 감소하였으며, 혈림프동의 확장과 함께 격막의 파괴가 관찰되었다. 노출 20주째 세포질내의 공포형성, 핵응축과 같은 변화가 관찰되었고, 결합조직층에서는 근섬유다발의 분절이 부분적으로 관찰되었다. 노출 28주째 상피층의 부분적인 붕괴, 섬모의 변화 및 섬모세포에 존재하는 미세구조가 괴사되었다. 결합조직층에 존재하는 다수의 근형질막은 팽창되었으며, 막의 내부에는 근섬유들이 대부분 파괴되어 막 주변에 근섬유 잔여물들이 관찰되었다. 노출 36주째에는 섬모들은 모두 탈락되고, 핵 응축되고, 이형소체의 증가가 나타나는 퇴행성 상피세포가 나타났다. 외부상피층의 상피세포들이 괴상되어 핵이 파괴되고, 세포질 내에는 다수의 용해소체들이 가득 차 있었으며, 미세융모들은 대부분 소실되었다. 따라서 만성적인 TBTCl 노출은 대복의 외투막은 각장성장 및 생리적인 기능의 장애를 유발하는 것으로 생각된다.
마두영약침(馬兜鈴藥鍼)의 천식억제(喘息抑制) 및 면역조절효과(免疫調節效果)에 대(對)한 실험적(實驗的) 연구(硏究)
박정준,김영일,이현,Park, Jung-Jun,Kim, Young-Il,Lee, Hyun 경락경혈학회 2005 대한침구의학회지 Vol.21 No.1
Objectives : The aim of this study was to investigate the Asthma-suppressive and Immune-regulatory effect of AF-HA(Aristolochiae Fructus Herbal-acupuncture) at Joksamni(St36) in OVA(ovalbumin) induced asthma mouse model. Methods : C57BL/6 mice were sensitized and challenged with OVA(ovalbumin) for 12 weeks(once a week). The mice in the OVA-AF-HA group were treated with AF-HA at St36 for the later 8weeks(3times/week). The mice in the OVA-Needle-prick group were treated with single prick with an injection needle at St36 for the later 8 weeks(3times/week). Results : 1. The lung weight and the total cells in lung of the mice treated with AF-HA at St36 decreased significantly compared with those of the OVA-control group. 2. Total leukocytes and eosinophils in BALF of the mice group treated with AF-HA at St36 decreased remarkably compared with those of the OVA-control group. 3. The collagen accumulation in lung of OVA-AF-HA group decreased significantly compared with that of the OVA-control group, 4. The concentrations of IL-4, IL-5, IgE in BALF, and IL-4, IL-5, IL-13 in serum of the mice group treated with AF-HA at St36 decreased significantly compared with those of the OVA-control group. 5. The numbers of $Gr-1^+/CD11b^+\;and\;CD11b^+$ cells in lung of the mice group treated with AF-HA at St36 decreased significantly compared with those of the OVA-control group. 6. The numbers of $CCR3^+,\;CD4^+,\;CD8^+\;and \;CD3e^+/CD69^+$ cells in lung of the mice group treated with AF-HA at 5136 decreased significantly compared with those of the OVA-control group. 7. The mRNA expressions of $TNF-{\alpha}$, IL-4, IL-5, IL13 in lung of the mice group treated with AF-HA at St36 decreased significantly compared with those of the OVA-control group. Conclusion : These results suggest that Aristolochiae Fructus Herbal-acupuncture at Joksamni(St36) may be an effictive therapeutic method to treat asthma.
박정준,박명애,최혜승,김석렬,Park, Jung-Jun,Park, Myoung-Ae,Choi, Hye-Sung,Kim, Seok-Ryel 한국현미경학회 2011 Applied microscopy Vol.41 No.3
조피볼락, Sebastes schlegeli 소화관 주변에는 다수의 아니사키 스가 기생하고 있었으며, 몇몇 개체들은 간의 표면에 존재하고 있었다. 유전학적 결과 조피볼락에 기생한 아니사키스는 3종으로 분석되었으며, Hysterothylacium sp.가 가장 많이 분포하고 있었다. Hysterothylacium sp.는 Anisakis simplex보다 짧고 가늘었다. 두 종 모두 전방부분에는 lip, 입, 신경환, 분비공, 분비관이 관찰되었다. 두 종 모두 중간부분에는 식도, 위, 장이 존재하고 있었 지만, intestinal ceacum과 ventricular appendage는 Hysterothylacium sp.에서만 관찰되었다. Hysterothylacium sp.의 후방부분 끝에는 conical nodulose apex이 관찰되었지만, A. simplex의 후방부분 끝에는 spine이 관찰되었다. SEM으로 관찰한 결과 Hysterothylacium sp.의 입 주위에는 3개의 lip이 존재하고 있었는데, dorsal lip은 넓이 65 ${\mu}m$ 내외였고, 한쌍의 lateroventral lip은 넓이 60 ${\mu}m$ 내 외였다. Lip의 표면에는 넓이 8 ${\mu}m$ 내외의 유두돌기 한쌍이 존재하고 있었다. Hysterothylacium sp.와 A. simplex의 충체 넓이는 각각 480 ${\mu}m$와 900 ${\mu}m$ 내외로 측정되었다. Hysterothylacium sp. 의 lateral alae는 높이 7 ${\mu}m$ 내외였으며, cornical nodulose apex에 존재하는 돌기는 지름 약 3.3 ${\mu}m$였다. A. simplex의 후방부분 끝 에는 높이 약 20 ${\mu}m$의 spine이 관찰되었다. 조피볼락 장의 내강 에 존재하는 아니사키스들은 대부분 Hysterothylacium sp.였고, 점막주름 근처 혹은 점막하층에 기생하고 있었다. 아니사키스의 기생으로 인하여 숙주의 점막상피에는 다수의 점액세포가 증가하였다. Numerous anisakids were parasitic near the digestive tract of the black rockfish, Sebastes schlegeli and some anisakids observed on the liver of the host. Anisakids in the host were identified three species (Hysterothylacium sp., Anisakis simplex, A. pegreffii) and Hysterothylacium sp. was the high occurrence of anisakid worms in the host. Hysterothylacium sp. was shorter and thinner than A. simplex. Both of anisakids observed lip, mouth, nerve ring, excretory pore and excretory duct in the anterior portion. In the mid portion, anisakids had esophagus, ventriculus and intestine and especially, Hysterothylacium sp. had intestinal ceacum and ventricular appendage. There was conical nodulose apex at the end of the posterior portion in Hysterothylacium sp. and spine in A. simplex. SEM examination revealed that there was three lip near the mouth of Hysterothylacium sp. Dorsal lip was approximately 65 ${\mu}m$ and paried lateroventral lip were approximately 60 ${\mu}m$ in the width. All lips were found double papilla (approximately 8 ${\mu}m$ in the width). The body width of the Hysterothylacium sp. and A. simplex was approximately 480 ${\mu}m$ and 900 ${\mu}m$ respectively. The hight of the lateral alae was about 7 ${\mu}m$ and width of papilla on the cornical nodulose apex was about 3.3 ${\mu}m$ in Hysterothylacium sp. The hight of spine was approximately 20 ${\mu}m$ in A. simplex. There was mainly Hysterothylacium sp. in the intestinal lumen of the host. The nematod worms were parasitic near the mucosal fold and in the submucosal. In the mucosal epidermal layer, it was increased mucous cells by the infection of the parasites.
박정준 ( Park Jung-jun ),박기준 ( Park Gi-joon ),김원우 ( Kim Won-woo ),문재흠 ( Moon Jae-heum ),김성욱 ( Kim Sung-wook ) 한국구조물진단유지관리공학회 2017 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.21 No.2
In case of high performance fiber reinforced cementitious composite(HPFRCC) specimens with steel fiber of 30mm they exhibited poorer flexural strength than the straight fibers at a higher than 1.5% compared to steel fiber of 13mm and 19.5mm. In this study, Therefore we evaluated the flexural strength and cracking behavior of HPFRCC with steel fiber type such as fiber length, volume fraction.
잡음 분산 추정을 위한 개선된 Partial Sample Average 알고리즘
박정준(Park Jung-Jun),이진용(Lee Jinyong),임태민(Lim Taemin),김영록(Kim Younglok) 한국방송·미디어공학회 2010 한국방송공학회 학술발표대회 논문집 Vol.2010 No.11
잡음 분산 값은 SNR(signal-to-noise ratio) 추정이나 MMSE(minimum mean square error) 계산, 채널 임펄스 응답의 추정 등에 사용되는 중요한 파라미터이다. 채널이 시간에 따라 변하는 무선 통신 환경에서, 신호와 섞여 있는 잡음과 간섭 신호의 정확한 추정에는 그 한계가 있으며 이로 인해 발생하는 추정 오차는 수신기의 데이터 검출 성능을 저하시킨다. 훈련열을 이용하여 채널을 추정하였을 경우 추정된 채널 임펄스 응답 신호 중 다중 경로 신호는 소수에 불과하고 나머지 대부분의 계수는 잡음 성분만을 포함하는 신호이다. 이러한 특징을 이용하여 채널의 추정 계수로 잡음 분산을 추정하는 방법이 기존에 제시되어 있다. 여기서 제안하는 알고리즘은 기존 알고리즘인 PSA(partial sample average)와 비교해 연산량에서 차이가 거의 없이 구현되며, 3GPP TDD[1]에서의 모의 실험을 통하여 기존 알고리즘보다 더 정확한 분산 값을 찾아냄을 확인하였다.
전기촉진시험 방법에 의한 혼화재 혼입 콘크리트의 염화물 저항성 평가에 관한 연구
박정준 ( Park¸ Jung-jun ),김도겸 ( Kim¸ Do-gyeum ),고경택 ( Go¸ Gyung-taek ),김성욱 ( Kim¸ Sung-wook ),하진규 ( Ha¸ Jin-gyu ) 한국구조물진단유지관리공학회 2002 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.6 No.2
The test method ASTM C 1202 is critically examined in this paper. It is observed that the total charge passed is not a good basis for classification of concrete quality when different binders(such as fly-ash, bottom ash and silica fume etc etc) are considered. A change of voltage and simple modification test method are examined. The modification involved performing of additional test using distilled water as electrolyte. The difference in the total charges passed obtained between change of voltage in concrete and test performed in water is suggested as an indicator for concrete quality. The chloride ion penetration for concrete with mineral admixtures can correctly estimated in considring pozzolan reacts with long term ages of the concrete.
플라이애시를 사용한 콘크리트의 염화물 저항성 촉진시험 방법에 관한 검토
박정준 ( Park¸ Jung-jun ),구현본 ( Koo¸ Hyun-bon ),김도겸 ( Kim¸ Do-gyeum ),김성욱 ( Kim¸ Sung-wook ),하진규 ( Ha¸ Jin-gyu ) 한국구조물진단유지관리공학회 2002 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.6 No.1
The test method ASTM C 1202 "Electrical indication of concrete’s ability to resist chloride ion penetration" is critically examined in this paper. It is observed that the total charge passed is not a good basis for classification of concrete quality when different binders (such as fly-ash etc) are considered. A simple modification test method is proposed. The modification involved performing of additional test using distilled water as electrolyte. The difference in the total charges passed obtained between ASTM C 1202 test and test performed in water is suggested as an indicator for concrete quality. By using the modified total charge passed, improvements in relationship to direct measurements of chloride ion penetration are observed.