명태(Gadus chalcogrammus)의 일차 간세포 배양에서 온도 스트레스에 따른 HSP70 mRNA와 단백질 발현

김소선,이창주,박장수,Kim, So-Sun,Lee, Chang-Ju,Park, Jang-Su 한국환경과학회 2020 한국환경과학회지 Vol.29 No.6

Water temperature is one of the most important factors of fish survival, affecting the habitat, migration route, development, and reproduction. This experiment studied the induction level of heat shock protein (HSP70) mRNA and protein in a walleye pollock (Gadus chalcogrammus) primary hepatocyte culture based on different temperatures. Hepatocytes were attached at 7.5℃ for 24 hours. Hsp70 induction levels were then measured for 48 hours at 5, 8, 11, 14, and 17℃. The induction level was lowest at 5℃ and generally increased with temperature until 14℃. The induction level was reduced at 17℃, indicating that 14℃ is the highest tolerable temperature for hepatocytes. These data indicate that primary hepatocyte cell culture is under no stress at 5 and 8℃. Temperatures greater than 11℃ induce stress, showing similar induction patterns in both mRNA and protein in hepatocytes. The results suggest that 14℃ is the maximum internal defense temperature of walleye pollock survival.

경량화 시스템에 적합한 유한체 $GF(2^m)$에서의 고속 역원기

김소선,장남수,김창한,Kim, So-Sun,Chang, Nam-Su,Kim, Chang-Han 대한전자공학회 2005 電子工學會論文誌-SD (Semiconductor and devices) Vol.42 No.9

The design of efficient cryptosystems is mainly appointed by the efficiency of the underlying finite field arithmetic. Especially, among the basic arithmetic over finite field, the rnultiplicative inversion is the most time consuming operation. In this paper, a fast inversion algerian in finite field $GF(2^m)$ with the standard basis representation is proposed. It is based on the Extended binary gcd algorithm (EBGA). The proposed algorithm executes about $18.8\%\;or\;45.9\%$ less iterations than EBGA or Montgomery inverse algorithm (MIA), respectively. In practical applications where the dimension of the field is large or may vary, systolic array sDucture becomes area-complexity and time-complexity costly or even impractical in previous algorithms. It is not suitable for low-weight and low-power systems, i.e., smartcard, the mobile phone. In this paper, we propose a new hardware architecture to apply an area-efficient and a synchronized inverter on low-complexity systems. It requires the number of addition and reduction operation less than previous architectures for computing the inverses in $GF(2^m)$ furthermore, the proposed inversion is applied over either prime or binary extension fields, more specially $GF(2^m)$ and GF(P) . 효율적인 암호 시스템의 설계는 환경에 적합한 유한체 연산이 뒷받침되어야 한다 특히 유한체에서의 역원 연산은 다른 연산에 비해 가장 많은 수행시간을 소비하므로, 개선에 대한 연구가 활발히 진행되고 있다. 본 논문에서는 다항식 기저를 기반으로 Extended binary god algorithm (EBGA)를 이용한 유한체 $GF(2^m)$에서의 고속 역원 알고리즘을 제안한다. 제안된 역원 알고리즘은 EBGA보다 $18.8\%$, Montgomery inverse algorithm (MIA)보다 $45.9\%$ 적은 수행횟수를 가진다. 또한 기존에 제안된 시스톨릭 어레이 구조 (Systolic array structure)는 유한체 차수 m이 증가하는 경우 많은 하드웨어 리소스가 요구된다. 따라서 스마트 카드나 모바일 폰 등과 같은 경량화와 저전력이 요구되는 환경에는 적용하기 힘들다. 본 논문에서는 경량화된 암호 시스템 환경을 바탕으로 공간복잡도가 적으면서 동기화된 연산을 수행하는 새로운 하드웨어 구조를 제시한다. 본 논문에서 제안된 하드웨어 구조는 유한체 $GF(2^m)$에서의 역원을 계산하기 위해 기존의 알고리즘보다 적은 덧셈 연산과 모듈러 감산 연산을 포함하고 있으며, 유한체 $GF(2^m)$와 GF(p)에 적용이 가능한 통합된 역원기이다.

Comparison of Thermal Stress Induced Heat Shock Factor 1 (HSF1) in Goldfish and Mouse Hepatocyte Cultures

So-Sun Kim(김소선),Jae-Hyeong So(소재형),Jang-Su Park(박장수) 한국생명과학회 2016 생명과학회지 Vol.26 No.12

Heat shock proteins (HSPs)은 다양한 생리학적인 또는 환경적 스트레스에 응답하여 유도된다. 그러나 HSPs의 전사 활성은 heat shock factors (HSFs)에 의해 조절 된다. 현재 연구에서는 붕어와 마우스의 간세포 배양에서 열 스트레스에 의한 heat shock factor 1 (HSF1)의 패턴 차이와 heat shock protein 70 (HSP70)의 발현을 면역분석법을 이용하여 조사하였다. 붕어의 간세포는 33°C에서 trimer를 이루지만 마우스의 간세포는 42°C에서 trimer를 이루었다. 이 연구는 붕어와 마우스의 HSF1은 열 스트레스로부터 다른 온도에서 반응을 한다는 것을 보여준다. 또한 재조합 단백질을 이용하여 붕어와 인간의 HSF1의 온도에 따른 활성 조건을 CD spectroscopy와 면역분석을 이용하여 조사하였다. 이러한 결과들은 인간과 마우스 HSF1과 붕어의 HSF1은 온도에 의한 활성 변화를 보이지만 그들의 최적 활성 온도는 다르다는 것을 알 수 있다. Heat shock proteins (HSPs) are induced in response to various physiological or environmental stressors. However, the transcriptional activation of HSPs is regulated by a family of heat shock factors (HSFs). Fish models provide an ideal system for examining the biochemical and molecular mechanisms of adaptation to various temperatures and water environments. In this study, we examined the pattern differentials of heat shock factor 1 (HSF1) and expression of heat shock protein 70 (HSP70) in response to thermal stress in goldfish and mouse hepatocyte cultures by immune-blot analysis. Goldfish HSF1 (gfHSF1) changed from a monomer to a trimer at 33°C and showed slightly at 37°C, whereas mouse HSF1 (mHSF1) did so at 42°C. This experiment showed similar results to a previous study, indicating that gfHSF1 and mHSF1 play different temperature in the stress response. We also examined the activation conditions of the purified recombinant proteins in human HSF1 (hmHSF1) and gfHSF1 using CD spectroscopy and immune-blot analysis. The purified recombinant HSF1s were treated from 25°C to 42°C. Structural changes were observed in hmHSF1 and gfHSF1 according to the heat-treatment conditions. These results revealed that both mammal HSF1 (human and mouse HSF1) and fish HSF1 exhibited temperature-dependent changes; however, their optimal activation temperatures differed.

중환자 전문간호사의 세부영역 분류체계 및 전문간호사의 핵심역량 개발

김소선 ( So Sun Kim ),유옥수 ( Ock Su You ),권인각 ( In Gak Kwon ),문성미 ( Seong Mi Moon ),성영희 ( Young Hee Sung ) 병원간호사회 2006 임상간호연구 Vol.11 No.1

청소년의 올바른 항생제 사용을 위한 웹 기반 교육프로그램 개발 및 평가

김소선(Kim, So-Sun),천주영(Cheon, Joo Young),권인숙(Kwon, In Sook),조윤미(Cho, Yoon Mi),문성미(Moon, Seongmi) 기본간호학회 2011 기본간호학회지 Vol.18 No.3

Purpose: This study was done to develop a web-based education program on appropriate antibiotic use and test the effects of the program on knowledge and attitudes towards antibiotic use in Korean adolescents. Methods: The web-based education program was developed through an extensive literature review and professional advisory meetings including technical assistance from a web-based education programmer and content experts. A convenience sample of 851 students from middle and high schools participated in the assessment of effects of the program. Knowledge and attitudes of the students towards antibiotic use and satisfaction with the web-based education program were measured. Descriptive statistics and paired t-test were used to analyze the data. Results: There were significant improvements in knowledge and attitudes towards antibiotic use following self-learning via the web-based education program in both middle and high school students. High school students demonstrated higher scores in knowledge and attitudes than middle school students. Conclusion: The results of this study indicate that this web-based education program on appropriate antibiotic use is a convenient and effective medium for self-learning in adolescents. Therefore the web-based program should be put into wide use as an effective and convenient teaching method for health education in secondary schools.

재활 2 : 서평

김소선(So Sun Kim) 연세대학교 간호정책연구소 1998 간호학탐구 Vol.7 No.2

간호사의 개인적 특성과 건강상태 인지, 신체상, 건강증진행위간의 관계

김소선(Kim So Sun),박정숙(Park Jeong Sook),노영숙(Roh Young Sook) 한국성인간호학회 2005 성인간호학회지 Vol.17 No.1

청소년의 항생제 사용에 대한 지식과 태도

김소선(Kim So-sun),문성미(Moon Seongmi),이은숙(Lee Eun Sook) 기본간호학회 2009 기본간호학회지 Vol.16 No.4

Purpose: The aim of this study was to identify knowledge and attitudes on antibiotic use held by adolescents (middle and high school students) in Korea. Methods: Data were collected from a convenience sample of 651 students residing in 5 major cities in Korea. The questionnaire was developed after an extensive literature review and professional advisory meetings. Descriptive statistics, t-test, chi square test, ANOVA, and Pearson correlation coefficients with the SPSS program were used to analyze the data. Results: Respondents correctly answered 6.23 antibiotic-related questions out of 15 questions. Two thirds of the respondents knew viruses caused most colds and coughs, but 80% did not know that antibiotics do not work against viruses. High school students' attitudes towards antibiotics were somewhat negative. More middle school students than high school students asked doctors for antibiotics for a cold. High school students did not check if antibiotics were included in their prescription for a cold. There was no significant correlation between knowledge and attitudes towards antibiotic use. Conclusion: Education programs on appropriate antibiotic use need to be developed for these students. Continual reinforcement and repetition of the contents are required to change attitudes.

SimMan 시뮬레이션 학습 시나리오의 개발 및 학습 수행 평가

고일선(Ko Il-Sun),김희순(Kim Hee-Soon),김인숙(Kim In-Sook),김소선(Kim So-Sun),오의금(Oh Eui-Gum),김은정(Kim Eun-Jung),이주희(Lee Ju-Hee),강세원(Kang Se-Won) 기본간호학회 2010 기본간호학회지 Vol.17 No.3

Purpose: The purpose of this study was to develop a scenario and evaluate students' performance in simulation learning of care for patients with asthma in emergency units. Methods: Meetings of experts were used to develop a scenario based on actual patients and textbook material. An evaluation protocol was developed to evaluate the simulation learning. The scenario was used in 2006 with six groups of 26 senior nursing students who participated voluntarily. Results: The scenario was developed according to the nursing process for 15 minutes of simulation learning. The nursing students were able to demonstrate their knowledge and skills. The results showed a need to improve problem solving ability. In the self-evaluation, the students reported that simulation learning helped them to integrate their knowledge to practice and recognize their weaknesses and strengths. However, the scores for self-confidence about patient care after the simulation learning were low (4.8/10). Conclusion: The scenario in this study gave the students the experience of providing qualified and secure nursing care under conditions similar to reality. Further development of a variety of scenarios for simulation learning is needed.

간이식 수혜자의 자가간호이행, 사회적 지지, 생리학적 지표

김현경(Kim, Hyunkyung),최모나(Choi, Mona),김소선(Kim, So Sun),김순일(Kim, Soon-il) 한국성인간호학회 2015 성인간호학회지 Vol.27 No.2