육계 사료 내 매실 및 오미자 첨가가 성장, 혈액 생화학 성분 및 항산화 작용에 미치는 영향

고영현(Young-Hyun Ko),문양수(Yang-Soo Moon),손시환(Sea-Hwan Sohn),정장용(Chang-yong Jung),장인석(Insurk Jang) 韓國家禽學會 2012 韓國家禽學會誌 Vol.39 No.2

To investigate the effect of dietary supplementation of freeze-dried plum (Prunus mume Siebold and Zucc., PMS) or omija meal (Schizandra chinensis Baill.; SCB) on growth performance, organ weights, blood biochemical profiles and antioxidant defense system, a total of 96, 3-day-old male broiler chickens were assigned to three dietary groups: (1) control diet, (2) control diet supplemented with PMS at 0.2%, (3) control diet supplemented with SCB at 0.2%. In vitro antioxidant activity, plum and omija extracts showed a significantly higher radical scavenging activity (RSA). In particular, omija extract showed much higher RSA than plum extract. Dietary addition of plum or omija did not affect body weight, feed intake, feed conversion and the relative weight of digestive organ in birds. Plasma triglyceride significantly (P<0.05) increased in birds fed the diet supplemented with omija compared with those fed control diet without affecting the other blood biochemical components. Furthermore, reduced form of glutathione (GSH) in the liver or muscle significantly (P<0.05) increased in birds fed the diet fortified with plum and omija. However, the specific activities of superoxide dismutase (SOD), glutathione peroxidase (GPX) and glutathione-S-transferase (GST) and MDA (malondealdehyde) in the intestine, liver and muscle were not altered by dietary antioxidant sources. In conclusion, dietary plum and omija resulted in a positive effect on some antioxidant indicators such as increased in vitro RAS in extracts and in vivo GSH level in the liver and muscle without affecting growth performance. Therefore, dietary addition of 0.2% of plum or omija could be applicable as potential antioxidant sources in broiler chick production.

사료 내 은행잎과 호박 첨가 급여가 육계의 성장, 소장 내 미생물 균총, 혈액 생화학 분석 및 체조직 내 항산화 작용에 미치는 영향

고영현(Young-Hyun Ko),이성실(Seong-Sil Lee),장인석(In-Surk Jang) 韓國家禽學會 2010 韓國家禽學會誌 Vol.37 No.1

은행잎 및 호박 분말 첨가가가 육계의 성장, 혈액내 생화학분석, 체 조직내에 항산화 방어 체계 및 소장내 미생물 균총에 미치는 영향을 조사하기 위하여 3일령 육계 120수를 각 처리구당 24수씩 대조구(CON) 및 은행잎 2.5%(GK2.5), 5.0%(GK5.0)와 호박 2.5%(PK2.5), 5.0%(PK5.0) 등 5처리구에 완전 임의 배치하여 사양 시험을 실시하였다. 전체 시험 기간(3∼35일령)의 사료 섭취량은 PK2.5구에서 GK5.0 및 PK5.0구들에 비해 유의하게(P<0.05) 낮았으며, 사료 요구율은 은행잎과 호박을 각각 5.0% 급여한 GK5.0구와 PK5.0구가 다른 처리구들과 비교하여 유의적으로(P<0.05) 증가되었다. 혈액내 중성지방은 PK5.0구가 다른 첨가구들보다 높았으며(P<0.05), ALT는 GK5.0 및 PK5.0구에서 GK2.5구보다 현저히(P<0.05) 높았다. 소장점막세포의 SOD 및 GSH-Px 활성도는 은행잎 및 호박 첨가 급여구가 대조구보다 유의적으로 증가되었다(P<0.05). 간 조직에서는 GK2.5구에서 대조구에 비해 SOD, GST 및 GSH-Px 활성도가 유의적으로(P<0.05) 증가되었다. 또한 근육조직에서도 GST 활성도는 은행잎 및 호박 첨가 급여구가 대조구보다 유의적으로(P<0.05) 높았다. 그러나 소장점막세포 및 근육조직의 지질과산화(MDA) 수준은 PK5.0구에서 타 처리구에 비해 증가되는(P<0.05) 결과를 보였다. 장내 소화물의 총 E. coli 균총(CFU)은 은행잎이나 호박을 첨가한 급여구에서 대조구에 비해 현저히(P<0.05)감소되었다. 이상의 결과를 보아, 은행잎 및 호박 분말 2.5%수준은 육계의 소장 점막세포, 간 및 근육조직의 특정 항산화효소 활성도를 증가시키며, 대장에서 대장균 총균수를 감소시켜 육계의 사료내 천연 항산화 소재로서 이용이 가능할 것으로 사료된다. The current study was performed to develop natural bio-active substances as additives for the production of high quality broiler chickens. A total of 120 male 3 day-old broiler chicks were randomly allocated to CON (control), GK2.5 (ginkgo leaf 2.5%), GK5.0 (ginkgo leaf 5.0%), PK2.5 (pumpkin 2.5%) and PK5.0 (pumpkin 5.0%) of five groups in cages (24 birds per group). All birds were fed corresponding diets from 3 to 35 d of age and determined growth performance and biological parameters including blood biochemical profiles, antioxidant status and intestinal microflora. During the entire feeding trial, GK5.0 and PK5.0 groups resulted in a significantly (P<0.05) higher FCR than GK2.5 and PK2.5 groups. Plasma triglyceride significantly (P<0.05) increased in GK5.0 group compared with the other groups, and the level of alanine transaminase (ALT) increased (P<0.05) in GK5.0 and PK5.0 groups compared with that in PK2.5 group. Dietary addition of ginkgo leaf and pumpkin significantly (P<0.05) increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the small intestine. Also, the addition of 2.5% ginkgo leaf significantly (P<0.05) increased the activities of SOD, GSH-Px and glutathione-S-transferase (GST) in the liver. Futhermore, muscle GST activity significantly (P<0.05) enhanced by dietary addition of ginko leaf and pumpkin. However, the level of lipid peroxidation (MDA) in the small intestine and muscle turned to be higher (P<0.05) in PK5.0 group. The colony forming units (CFU) of E. coli in intestinal digesta significantly (P<0.05) decreased in both ginko leaf and pumpkin supplemented groups compared with CON group. In conclusion, dietary addition of 2.5% ginko leaf and pumpkin as dietary sources can be applicable for the production of high quality broiler chickens.

췌장에서 발생한 낭성종양 159예의 임상적 고찰

고영현 ( Young Hyun Ko ),한덕종 ( Duck Jong Han ),이승규 ( Sung Gyu Lee ),이영주 ( Young Joo Lee ),박광민 ( Kwang Min Park ),황신 ( Shin Hwang ),임덕상 ( Duck Sang Lim ),김송철 ( Song Cheol Kim ) 대한소화기학회 2003 대한소화기학회지 Vol.41 No.1

Background/Aims: The aim of this study was to review the cystic neoplasms of the pancreas. Methods: We retrospectively reviewed the clinical records of 159 patients with cystic neoplasms of the pancreas. The patients were treated surgically at the Asan Medical Center between March 1991 and May 2002. Results: There were 37 serous cystadenomas (23%), 24 mucinous cystic neoplasms (15%), 38 solid and papillary epithelial neoplasms (24%), and 60 intraductal papillary mucinous tumors (38%). The most common symptom was abdominal pain. Ultrasonography and computed tomography were used for diagnosis in 63.6% and 71.6% of the patients, respectively. Regarding surgical treatments, pancreaticoduodenectomy was performed in 25 cases, pylorus preserving pancreticoduodenectomy in 42 cases, total pancreatectomy in 11 cases, distal pancreatectomy in 60 cases, spleen preserving distal pancreatectomy in 4 cases, segmental pancreatectomy in 14 cases, and local excision in 3 cases. The overall perioperative complication rate was 12%. The 32 patients had malignacy on histologic examinations. The overall 5 year survival rate was 90.8%. Conclusions: For the cystic neoplasms of the pancreas we recommend an active strategy of resection considering the high possibility of malignancy and the diagnostic difficulties. The resection has usually led to a low morbidity, low mortality, and good prognosis. (Korean J Gastroenterol 2003;41:49-58)

커피박 첨가가 육계의 소장 효소 활성도, 생화학 지표 및 장내 미생물 균총에 미치는 영향

고영현(Young-Hyun Ko),윤서현(Seo-Hyun Yun),송민혜(Min-Hae Song),김세윤(Se-Yun Kim),김종선(Jong-Sun Kim),김현욱(Hyoun-Wook Kim),장인석(In-Surk Jang) 한국가금학회 2014 韓國家禽學會誌 Vol.41 No.2

육계에서 품종 및 사육 밀도가 생산성 및 생리적 적응성 지표에 미치는 영향

장인석,윤서현,고영현,김세윤,송민혜,김종선,손시환,문양수,Jang, In-Surk,Yun, Seo-Hyun,Ko, Young-Hyun,Kim, Se-Yun,Song, Min-Hye,Kim, Jong-Sun,Sohn, Sea-Hwan,Moon, Yang-Soo 한국가금학회 2014 韓國家禽學會誌 Vol.41 No.3

본 실험은 육계 품종(Ross vs. Cobb) 및 사육 밀도(표준 밀도구, $0.046m^2$/수 vs. 고밀도구, $0.023m^2$/수)가 생산성, 장기무게, 혈액 corticosterone, 친염증 사이토카인과 항산화 효소의 mRNA 발현에 미치는 영향을 조사하기 위해 실시하였다. 전체 사육 기간 동안 품종에 따른 생산성 지표들은 차이가 없었으나, 사육 밀도에 따른 사양 성적은 두 품종 모두 고밀도 사육구(HD)에서 표준 밀도구(SD)에 비해 체중과 사료 섭취량이 유의적으로 감소하였다(P<0.05). 간과 비장의 상대적 무게는 품종에 따른 유의적 차이가 나타났으나(P<0.05), 사육 밀도에 따른 차이는 없었다. 혈액 corticosterone 농도는 품종 및 사육 밀도에 따른 통계적 차이는 없는 것으로 나타났다. 육계 품종 및 사육 밀도에 따른 친염증 사이토카인과 항산화 효소의 mRNA 발현 양상으로 간 조직에서 이들 사이토카인(IL-$1{\beta}$, IL-6, IL-18 및 IFN-${\gamma}$) mRNA 발현은 품종과 사육 밀도에 따른 차이가 없었다. 한편, 항산화 유전자인 GPX mRNA 발현은 품종에 따라 현저한 반응의 차이를 보였으며(P<0.05), Ross 품종이 Cobb 품종보다 GPX mRNA 발현이 낮았다. 사육 밀도 요인에 따른 차이를 보면, SOD, GPX 및 CAT의 mRNA 발현이 모두 고밀도 사육구에서 현저히 증가되는 것으로 나타났다(P<0.05). 비장조직에서 대부분의 친염증 사이토카인은 품종 및 사육 밀도에 따른 차이가 없었으나, IL-18 mRNA 발현은 고밀도 사육 시 Cobb 종에서 발현이 감소되었다(P<0.05). 항산화 효소 mRNA 발현은 품종 및 사육 밀도 요인에 따라 SOD와 CAT에서 유의적 차이가 있었다(P<0.05). 이와 같은 결과로 보아 사양 성적은 육계 품종에 따른 요인에 영향을 받지 않았지만, 사육 밀도는 생산성을 감소시켰다. 친염증 사이토카인의 mRNA 발현은 품종 및 사육 밀도에 따른 차이는 적었으나, 항산화 효소는 품종, 사육 밀도 및 장기조직에 따른 발현의 차이가 나타나 체내 항상성 조절에 중요한 지표로서 지속적인 연구가 요구된다. The aim of this study was to determine the effects of stocking density and strain on the performance and physiological adaptive responses including the plasma corticosterone content and the level of mRNA expression of pro-inflammatory cytokines and antioxidant enzymes in broiler chicks. A total of 300 birds of two strains (150 Ross strain vs. 150 Cobb strain) aged 3-d old were allotted into two stocking densities (standard stocking density,$0.046m^2/bird$ vs. high stocking density, $0.023m^2/bird$) in battery cages by $2{\times}2$ factorial designs with ten replicates until 35 d of age. There was no significant strain effect on body weight, feed intakes and feed to gain ratio and the relative organ weights. However body weight, feed intakes and relative organ weight were found to be significantly (P<0.05) affected by the effect of stocking density. Plasma corticosterone level was not affected by both stocking density and strain effects. Hepatic mRNA expression of pro-inflammatory cytokines including interleukin-$1{\beta}$ (IL-$1{\beta}$), IL-6, IL-18 and interferon-gamma (IFN-${\gamma}$) was not significantly changed by the effects of strain and stocking density. However, the mRNA expression of glutathione peroxidase (GPX) was affected by strain, showing that Ross strain decreased (P<0.05) the GPX expression. With respect to the effect of stocking density, there was a significant (P<0.05) increase in superoxide dismutase (SOD) and catalase (CAT) and GPX mRNA expression in the liver from high stocking density group. Splenic pro-inflammatory cytokine expression was not also affected by stocking density and strain, except that IL-18 mRNA significantly (P<0.05) decreased in Cobb strain under high stocking density. The mRNA expression of SOD and CAT was significantly (P<0.05) affected by the effects of stocking density and strain. In conclusion, growth performance was not affected by strain but stocking density. Although mRNA expression of major pro-inflammatory cytokines was not changed by stocking density and strain, antioxidant enzyme was significantly affected by stocking density, strain or even organ in birds under summer conditions. More detailed studies still needed to be explored to elucidate the effects of environmental conditions and genetic background on physiological responses in birds.

산란계에 천연 항산화원으로서 가시오갈피 및 두충 급여가 체내 항산화 작용에 미치는 영향

강선영(Sun-Young Kang),이민희(Min-Hee Lee),고영현(Young-Hyun Ko),손시환(Sea-Hwan Sohn),문양수(Yang-Soo Moon),장인석(In-Surk Jang) 韓國家禽學會 2010 韓國家禽學會誌 Vol.37 No.1

본 연구는 하이라인 브라운 실용 산란계(20주령)를 이용하여 대조군 및 항산화원으로 가시오갈피잎 및 두충잎을 각각 사료 급여량 대비 0.5%와 1.0%를 첨가하여 모두 5군을 설정하여 산란초기에 10주 동안 사양하여 혈액내 항산화력 및 체조직의 항산화 작용에 미치는 영향을 비교 분석하였다. 항산화 생리 활성 물질의 급여(가시오갈피, 두충) 및 처리 수준(0.5%:1.0%)간에 체중 및 증체량의 변화는 없었다. 한편, 혈액내 항산화력은 식물성 항산화 물질 급여군에서 전체 항산화력(TAS)이 유의적으로(P<0.05) 증가하였으며, 특히 두충 급여군에서 높게(P<0.05) 나타났다. 소장 상피세포내의 GST의 활성도는 대조군보다 두충의 첨가 급여군에서 유의적으로 높게 나타났다(P<0.05). 체 조직에서 항산화 작용을 살펴보면, 먼저 소장 SOD 활성도는 가시오갈피 0.5% 첨가군과 두충 0.5% 첨가군이 대조군보다 유의적으로(P<0.05) 높게 나타났다. 간 조직내 항산화 효소의 활성도를 조사한 결과, 대조군과 가시오갈피 첨가군 간에는 특이적 변화가 없는 것으로 관찰되었다. 결론적으로 산란 초기에 가시오갈피와 두충잎 분말의 급여는 혈액내 항산화력 증진과 소장 점막 조직의 항산화 방어기능에 긍정적 영향을 미치는 것으로 사료된다. 본 실험에서 항산력 및 경제성 등을 고려할 경우 0.5% 수준의 식물성 항산화 물질의 급여가 동물체의 건강 증진으로 체내 항상성 유지에 가장 유리하게 적용될 수 있을 것으로 사료된다. To investigate the effect of dietary supplementation of Acanthopanax senticosus (AS) and Eucommia ulmoides (EU) on antioxidant defense system in laying hens, a total of three hundreds sixty 20-wk old Hyline brown commercial laying hens were assigned to five dietary groups for 10-wk: (1) control diet, (2) control diet supplemented with AS at 0.5%, (3) control diet supplemented with AS at 1.0%, (4) control diet supplemented with EU at 0.5% and (5) control diet supplemented with EU at 1.0%. Total antioxidant status (TAS) in blood and antioxidant enzymes including superoxide dismutase (SOD), gluthathione -S- transferase (GST) and glutathione peroxidase (GSH-Px), and lipid peroxidation in the small intestine and liver were measured. There were no changes in body weight for 10-wk dietary treatment. TAS in blood significantly (P<0.05) increased in birds fed the diet supplemented with 1% AS and 0.5 and 1.0% EU compared with those fed control diet. Especially, dietary EU showed much higher (P<0.05) TAS compared with AS. In the antioxidant defense enzymes, GST activity of the small intestine was shown to be significantly (P<0.05) increased in birds fed the diets supplemented with 0.5 and 1.0% EU compared with those fed the control diet. In addition, intestinal SOD activity significantly (P<0.05) increased in birds fed the diets supplemented with 0.5% of AS and EU. However, we could not observe any significant dietary treatment effect of those antioxidant parameters in the liver. In conclusion, dietary supplementation of 0.5% AS and EU in a laying hen diet could be applied as a potential antioxidant source to improves bio-activity of antioxidant and economical aspect in laying hens.

반추가축영양 : 한우 거세우의 비육후기 사료 형태에 따른 사양성적, 도체특성 및혈액성분 변화

조웅기 ( Woong Gi Cho ),이신자 ( Shin Ja Lee ),고영현 ( Young Hyun Ko ),장인석 ( In Surk Chang ),이성실 ( Sung Sill Lee ),문여황 ( Yea Hwang Moon ) 한국동물자원과학회(구 한국축산학회) 2013 한국축산학회지 Vol.55 No.5

To develop a diet for Hanwoo steers during the late fattening phase, three different feeding treatments were investigated: a group fed concentrate and rice straw separately(Formula feed), a group fed TMR during the whole late fattening period(TMR), and a group fed TMR plus concentrate(Formula-TMR). Thirty Hanwoo steers 20 months of age(average body weight=445kg) were allotted by a completely randomized design into three treatment groups(ten steers in each group). The impact of these treatments on the performance, carcass traits, and blood composition related to body fat synthesis was assessed. The average dry matter and TDN intake were greater in Formula-TMR compared to other treatments(p<0.05); however, body gain, carcass weight, meat production index, and fat color were not affected by dietary type. Blood characteristics, such as concentrations of retinol, insulin, and leptin were not affected by any of the treatments; however the marbling score of the longisimus muscle was significantly higher in Formula-TMR(p<0.05), which was relatively high in blood leptin concentration. The muscle texture, meat quantity index, and meat quality index were highest in the Formula feed, TMR, and Formula-TMR, respectively(p<0.05).

유비쿼터스 환경에서의 홈제어 융합 시스템 개발

윤달환(Dal-Hwan Yoon),배동주(Dong-Joo Bae),김형묵(Hyung-Mook Kim),권오훈(Oh-Hoon Kwon),고영현(Young-Hyun Ko),허정화(Jeng-Hwa Huh),김호균(Ho-Keun Kim) 대한전기학회 2006 대한전기학회 학술대회 논문집 Vol.2006 No.10

We have developed the convergence system that can control an appliance under ubiquitous circumstance. The system can display the GPS information received from the satellite, several control signal and mobile phone signal. In order to display the information between phone and the PC, we can control program command.

모래다짐말뚝(SCP) 개량지반의 응력분담비에 관한 연구

고영현,김병일,박용원 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

Lots of soil improvement methods has been developed in order to increase soil properties of soft ground such as newly reclaimed land The sand compaction pile(SCP), which forms a composite ground by driving sand piles into soft ground, is one of the commonly used soil improvement techniques especially for soft clay deposit. In desinging SCP method, it is very important to determine the stress concentration ratio of composite ground. In this study, 3-dimensional analyses were carried out using single and group pile models to evaluate the stress concentration ratio with the area replacement ratio and the stiffness ratio. PENTAGON_3D software is used to analyze the composite ground using modeling with single pile of unit cell concept and group piles. As a result, the distribution of the predicted stress concentration ration with the stiffness ratio(Es/Ec) and the area replacement ratio is around 2.5 ~ 3.5

飽和度에 따른 三軸壓縮强度의 特性 硏究

최명진,고영현,상현규,김팔규 忠南大學校 産業技術硏究所 1999 산업기술연구논문집 Vol.14 No.1

Lately,the study of the soil behavior and mainly related to the degree of saturation. But most soils in the ground actually exist not in the saturated condition but in the unsaturated one. The foundation bases adn the soils used for construction material are almost such unsaturated soil as granite soil in Korea. The griaxial test are used in order to examine the strength characters of the unsaturated soil in this research, reappeared in-situ field condition for the undaturated soil through the UU-test in the laboratory and examined strength character. The variation of shear strength accrding to the increase of the degree of saturation is measured as the result and the proposed formula are suggested