관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

김치에서 분리한 Lactobacillus sp. JC-7과 Lactobacillus acidophilus 88간의 Electrofusion 최적조건 설정

조영배,최현정,백형석,전홍기 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

김치 발효 숙성기간을 연장하고 신선도를 오랫동안 유지 할 수 있는 김치발효 starter의 개발을 목적으로 최적숙성기에 있는 김치로부터 bacteriocin생성능이 없는 유산균을 분리하여 동정하였으며, bacteriocin생성능이 우수한 L.acidophilus 88을 융합시키기 위해 electrofusion에 대한 최적 조건을 검토하였다. 분리균주는 Lactobacillus속으로 동정되어 Lactobacillus sp. JC-7이라 명명하였다. Electrofusion에 의해 생성된 융합주를 식별하기 위해 streptomycin(2.5㎎/ml)에 내성을 나타내는 Lactobacillus sp. JC-7변이주와 kanamycin(600㎍/ml)에 내성을 나타내는 L.acidophilus 88변이주를 분리하였다. Electrofusion을 100V/㎝, 120msec(72ohms,1670 capacitance) 에서 수행했을 때 융합효율이 가장 양호하였으며 전기장의 세기와 시간이 중가할수록 융합 효율이 현저히 감소하였다. 2가 양이온은 농도가 중가할수록 대체적으로 융합효율을 감소시키는 경향을 나타내었으나 1mM MGCl_2에서는 대조군에 비해 융합효율이 약간 증가하였다. PEG매개에 의한 융합법의 융합효율을 비교한 결과, 융합효율은 chemical fusion<electrofusion<electrofusion+20% PEG순을 나타내었다. Evaluation of Optimum Conditions for the Electrofusion between Lactobacillus sp. JC-7 Isolated from Kimchi and Lactobacillus acidophilus 88. Young-Bae Jo, Hyun-Jung Choi, Hyung-Suk Baik and Hong-Ki Jun*. Department of Microbiology, Pusan National University, Pusan 609-735,Korea-A lactic acid bacterium was isolated from kimchi. The isolated strain was identified as the genus Lactobacillus thrugh its morphological characteristics and named as Lactobacillus sp. JC-7. The optimum conditions for the electrofusion between streptomycin(2.5㎎/ml)resistant mutant of Lactobacillus acidophilus 88 and kanamycin(600㎍/ml) resistant mutant Lactobacillus sp. JC-7 were evaluated. The highest number of fusants were obtained at a capacitance value of 120msec(1670㎌), a field strength of 100V/㎝,and a pulse controller setting of 72Ω. The potimum pH of elecroporation buffer was 7.5 and the concentration of divalent cation was 1mM MG^2+. Electrofusants were efficiently obtained by addition 20% polyethylene glycol to electroporation buffer. The yield of fusion was better than that of using polyethylene glycol mediated chemical induction.

연령대별 요추 추간판탈출증 환자의 요근과 척추주위근 단면적에 대한 분석

공덕현 ( Deok Hyun Kong ),배광호 ( Kwang Ho Bae ),고호연 ( Ho Yeon Go ),강명진 ( Myung Jin Kang ),김준수 ( Jun Su Kim ),양기영 ( Kee Young Yang ),이재훈 ( Jae Hoon Lee ),은영준 ( Young Jun Eun ) 한방재활의학과학회 2010 한방재활의학과학회지 Vol.20 No.4

Objectives :This study was designed to evaluate the correlation of the cross-sectional areas(CSA) of paraspinalis(iliocostalis lumborum, multifidus, longissimus thoracis parts lumborum) and psoas muscle and sex, age, change of the VAS(visual analogue scale) and past history of chronic low back pain in patients suffering from HIVD(herniation of intervertebral disc). Methods :Medical records of 140 subjects with HIVD were retrospectively reviewed. The MRI(magnetic resonance imaging) findings on the patients were scanned and analyzed by PiView program to find the paraspinal and psoas muscle CSA and were evaluated by the correlation of sex, age, change of the VAS, and past history of chronic low back pain. Results :There was significant difference between groups in male and female, young-adults age and middle age, middle age and old age, young-adults age and old age. But the correlation of the paraspinal and psoas muscle CSA and past history of chronic low back pain were partially significant. On the other hand, the correlation of the paraspinal and psoas muscle CSA and change of the VAS were not significant. Conclusions :These results suggest that the cross-sectional areas(CSA) of paraspinalis and psoas muscle have relevance to sex and age.

Direct effectiveness of pneumococcal polysaccharide vaccine against invasive pneumococcal disease and non-bacteremic pneumococcal pneumonia in elderly population in the era of pneumococcal conjugate vaccine: A case-control study

Kim, Jong Hun,Chun, Byung Chul,Song, Joon Young,Kim, Hyo Youl,Bae, In-Gyu,Kim, Dong-Min,Choi, Young Hwa,Jun, Yoon Hee,Choi, Won Suk,Kang, Seong Hee,Kwon, Hyun Hee,Jeong, Hye Won,Kee, Sae Yoon,Hur, Jia Elsevier Ltd. 2019 Vaccine Vol. No.

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>While herd effects and serotype replacement by childhood pneumococcal protein conjugated vaccines (PCVs) continues to accumulate worldwide, direct effectiveness of 23-valent pneumococcal polysaccharide vaccine (PPV23) against pneumococcal diseases in the elderly has been challenged. We estimated the direct effectiveness of PPV23 in the elderly population.</P> <P><B>Methods</B></P> <P>For a hospital-based case-control study, cases of invasive pneumococcal disease (IPD) and non-bacteremic pneumococcal pneumonia (NBPP) (adults ≥ 65 years) were identified in 14 hospitals participated in the pneumococcal surveillance program from March 2013 to October 2015, following implementation of PPV23 national immunization program (NIP) for the elderly in the Republic of Korea. Controls matched by age, sex, and hospital were selected at ratios of 1:2 (IPD) or 1:1 (NBPP). Clinical data and vaccination records were collected. Vaccine effectiveness was calculated as (1-adjusted odds ratio) × 100.</P> <P><B>Results</B></P> <P>We enrolled 148 IPD and 557 NBPP cases, and 295 IPD and 557 NBPP controls for analyses. Overall effectiveness of PPV23 against IPD was 28.5% [95% confidence interval (CI) −5.8%–51.6%] and against NBPP was 10.2% (-15.1-30.6) in all patients ≥ 65 years. However, in subgroup analysis of patients aged 65–74 years, PPV23 was protective against IPD [effectiveness 57.4% (19.4–77.5)] and against NBPP [effectiveness 35.0% (2.3–56.7)]. Furthermore, serotype-specific effectiveness of PPV23 against IPD was 90.6% (27.6–98.8) for PPV23-unique serotypes and 81.3% (38.6–94.3) for PPV23 serotypes excluding serotype 3.</P> <P><B>Conclusions</B></P> <P>This study indicates that PPV23 with broad serotype coverage might be beneficial in preventing IPD and NBPP due to non-PCV13 serotypes in the young-elderly, with potentially increasing effectiveness in the setting of childhood PCV NIP.</P> <P><B>Highlights</B></P> <P> <UL> <LI> National immunization program for PPV23 for the elderly was implemented in Korea. </LI> <LI> PPV23 was effective against IPD and NBPP for young elderly patients aged 65–74. </LI> <LI> Effectiveness of PPV23 against IPD was higher for PPV23 unique serotypes. </LI> <LI> PPV23 was non-protective against IPD caused by PCV13 serotypes. </LI> </UL> </P>

SO₂감지를 위한 NASICON 고체전해질 가스센서의 제조 및 특성 평가

배재철,Liu, aiguo,이상태,전희권,방영일,이덕동,허증수 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1

김치 분리균인 Bacillus sp. JK-43이 생산하는 Cyclodextrin Glucanotransferase의 생산 및 특성

전홍기,배경미,김영희,백형석 부산대학교 유전공학연구소 2000 분자생물학 연구보 Vol.16 No.-

김치 시료로부터 자동산화되지 않으며 열 및 중성 pH에서 안정한 AA 유도체인 AA-2G를 생산할 수 있는 당전이활성을 가진 CGTase 생산균주를 분리하였고, 분리균주의 형태학적, 배양학적, 생리학적 성질 및 16s-rDNA sequences를 조사한 결과 그람 양성의 간균으로 호기성이며 내생포자를 형성하는 전형적인 중온성 Bacillus sp. JK-43으로 동정되었다. Bacillus sp. JK-43의 CGTase는 AA-2G 뿐만 아니라 AA-6G로 추정되는 물질을 함께 생산하였으며, 효소 최적생산조건은 1.0% soluble starch, 1.0% yeast extract, 1.0% Na_2CO_3, 0.1% K_2HPO_4 그리고 0.02% MgSO_4·7H_2O가 함유된 배지에서 pH 7.0, 37℃에서 26시간 동안 진탕배양하였을 때였다. 각종 당공여체에 따른 Bacillus sp. JK-43의 AA-2G 생산성을 조사한 결과 β-CD에서 가장 높은 AA-2G 생산성을 보였으며, 식혜제조폐액인 엿기름 및 밥당화액에서도 비교적 높은 AA-2G 생산성을 보였다. 또한 여러 가지 당수용체에 대한 JK-43의 CGTase의 당전이 반응을 검토한 결과 sucrose, mannitol 및 inositol에서 높은 당전이 수율인 70∼90%를 나타내었다. A bacterial strain, designated as JK-43, producing extracellular cyclodextrin hlucanotransferase (CGTase)[EC 2.4.1.19] was isolated from kimchi. The CGTase from isolated strain JK-43 showed the transglucosylation activity from soluble starch to L-ascorbic acid (AA) compared to those obtained from other strains. A main product formed by this reaction was identified as 2-O-α-glucopyranosyl L-ascorbic acid (AA-2G) by testing its susceptibility to α-glucosidase hydrolysis, the HPLC prodiles, and through the elementary analysis. The β-CD, γ-CD, potato starch and corn starch were identified to be suitable glucosyl donor for transglucosylation reaction on AA by CGTase. Acceptor specificity on AA-2G production was examined by use of AA, Iso-AA and AA-2P. Transglucosylation was observed toward AA-2P as well as AA. The microorganism isolated from kimchi was identified as a strain of Bacillus sp. JK-43 based on the maximal CGTase production was observed in a medium containing 1.0% soluble starch, 1.0% yeast extract, 1.0% Na_2CO_3, 0.1% K_2HPO_4, and 0.02% MgSO_4·7H_2O with initial pH 7.0. The strain was cultured at 37℃ for 26 hrs with reciprocal shaking.

Human Rotavirus 위장관염에 대한 임상적 고찰

배현,이홍준,서우철,양은석,문경래,박영봉 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

We observed clinical features of 161 patients of acute gastroenteritis who were adimitted to the Department of Pediatrics in the Chosun University Hospital from January 1. 1989 to December 31. 1993. The stool specimen on admission was tested for rotavirus Ag by ELISA(Rotazyme Ⅱ^R)method. The results are as follows : 1) HRV antigen was detected in 97 patients (58.4%) among 161 patients with gastroenteritis. 2) In monthly distribution. 77.7% of cases developed between October and December 3) The majority if patients were under 24month of age with the peak incidnce being between 6-12 month of age. 4) Mean frequency of diarrhea per day were 5.3±2.6 days in HRV antigen rhinorrhea(48.5%), cough(44.7%). Dehydration was noted 87.2% of cases and 93.9% Was isotonic dehydration. 6) The mean duration of hospitalization was 5.3±2.6 days. 7) AST and AIT elevation in HRV group were 91.5%, 42.6% respectively. AST and ALT elevation in Non-HRV group were 73.1%, 26.9% respectively. AST elevation between two group was not correlated statistically. but ALT concentration was significantly increased in HRV group above normal than Non-HRV group (p<0.05%).

觀光地호텔의 非需期對策 方案에 관한 硏究 : 釜山 海雲臺地域을 中心으로

배순철,전영상 공주영상정보대학 1998 논문집 Vol.5 No.-

일반적으로 호텔의 고유기능은 숙박이라 할 수 있는데, 시대의 변천에 따라 사회적인 측면에서 호텔의 기능과 역할이 중요시되고 다양화를 요구하게 되어 현대사회에서는 호텔이 지역사회의 중심지로서 자연스럽게 사교문화의 중심이 되고 있다. 이러한 사회적 요구에 발맞추어 호텔을 찾는 관광객들이 호텔을 제2의 안락하고 편안한 집으로써 안주 할 수 있도록 손색이 없는 서어비스 제공을 해야 하는 것이 호텔의 책임이라 하겠다. 또한 오늘날 관광지호텔은 사람들의 여가활동에 대한 새로운 인식과 건강에 대한 욕구로 인하여 급속하게 객실 이용율이 높아지고 있는 실정이며 몇 년 전에 비하여 경영상태도 훨씬 나아지고 있는 것이 사실이다. 특히 소득의 증대, 여가시간의 증가, 교통발달로 인한 시간의 단축으로 인하여 관광객이 놀랄 정도로 성장추세를 보이고 있으며, 또한 관광 호텔이 계속적으로 늘어나고 있다. 이와 같이 관광객의 증가에 비례하여 관광호텔의 발전을 가져온 것은 사실이나 아직도 호텔 마아케팅 활동이 미비한 상태에 있다고 본다. 본 연군에서는 호텔 마아케팅에 패하여 실증적인 고찰을 하였으며 그 중심은 제Ⅲ장에서 제시된 실태조사를 분석함으로서 현상을 규명하고 문제점과 활성화 방안을 모색하는데 주력하였다. 결론적으로 관광지호텔은 도시호텔과 시장여건 및 조건이 틀리므로 이에 대한 마아케팅 방법도 달라져야 하기 때문에 각 호텔특성에 알맞는 마아케팅 기능을 구사하여 합리적인 판매전략을 시도하여야 하며, 또한 위에서 열거한 활성화 방안을 수용할 수 있는 경영주의 결심과 결단이 있을 때 관광지 호텔의 전망은 장기적인 측면에서 매우 밝다고 생각된다.

안양천 등 관내하천의 수질오염분석 및 생태학적 분석에 관한 연구(II)

배준현,서정범,구본영 안양대학교 자연과학연구소 1997 自然科學硏究 Vol.4 No.-

안양천을 비롯한 10개 지천에 대한 환경오염 분석을 하였다. 전편(Ⅰ)에 이어 안양천의 관내지류인 산본천, 당정천, 오전천, 왕곡천, 통미천을 중심으로 하여 수질오염분석과 중금속오염도 조사 및 생태학적 분석에 대하여 연구하였다. 아울러 안양천 및 그 지류의 환경진단과 함께 하천을 살릴수 있는 몇가지 제안을 하였다. 안양천 및 지류의 수질오염분석결과 측정기간에 BOD 값이 평균 25.5 mg/l으로, 최저 17.0mg/l에서 최고 36.4mg/l으로 환경기준치를 초과하고 있다. COD는 평균 18.9mg/l으로 최저 12.7mg/l에서 최고 24.2mg/l로서 환경기준치인 5급수 수질을 초과하고 있으나 다른 수질의 지표에 비해 비교적 양호한 값을 나타내고 있다. SS는 평균 23.8mg/l으로 3급수 이내의 수질을 유지하고 있다. 총질소(T-N)는 평균치가 16.7mg/l으로 상류는 기준치 이하를 나타내고 있으나 중·하류는 5급수 이상의 높은 수치를 보이고 있어 제거대책이 강구되어야 할 것이다. 하천퇴적물에 대한 중금속 오염도 분석결과 5개항목 모두 기준치를 초과하여 높게 나타났으며 특히 아연(Zn)의 농도는 매우 심각한 것으오 분석되었다. 분석결과는 Zn>Cu>Pb>As>Cd순으로 나타났다. 생태학적 분석에 있어서는 안양천의 상류에서 하류에 이르기까지 어류는 서식하지 못하고 있으며 하류일부지역에서 오염에 강한 극소수 어류가 발견되었다. 식물도 하류로 갈수록 오염에 비교적 강한 식물이 군락을 이루고 있었다. Water pollution analysis about Anyangchun river and rivers within the province, investigation of a contamination level of heavy metal and ecological analysis were studies. The result of water pollution analysis Anyangchun river and its tributaries during the period of measure says BOD was average 25.5mg/l, ranging from minimum 17.0mg/l to maximum 36.4mg/l which exceeds and environmental standard level COD was average 18.9mg/l, ranging from minimum 12.7mg/l to maximum 24.2mg/lwhich exceeds fifth class of water quality, and environmental datum level, but represents relatively better value than the other base point of water quality. SS was average 23.8mg/l, which maintains water quality of below the third class. Total Nitrogen was average 16.7mg/l ; the upper water was seen below a base point, but the middle and lower showed exceeds fifth class of water quality. Therefore, a proper measure to remove nitrogen will have to be deviced. According to the analysis result of heavy metal contamination level about the river deposit, all of 5 items exceeded the environment base point. Specially, concentration of Zinc was considered very serious. The result of analysis was proven in the order to Zn>Cu>Pb>As>Cd. In ecological analysis, fish couldn't living from the upper water to the lower one in Anyang river with in province and a small minority of fish were discovered in part of the lower water. Hereby we suggested the alternative to decline water pollution in Anyang watercourse.

양방향 연상기억장치를 이용한 지능적 패턴 인식

전선배,임영도 동아대학교 공과대학 부설 한국자원개발연구소 1990 硏究報告 Vol.14 No.2

Enhancements of encoding and decoding strategy of a discrete bidirectional associative memory (BAM) proposed B. Kosko are presented. This paper describes multiple training, which allow to achieve recall of single trained pair under suitable initial conditions of data (We fixed Hamming distance). Computer simulations are shown to lead to an improvement in some instances over the original Kosko strategy for recall of multiple pairs.