연취급 근로자들의 건강증진에 미치는 호흡용 보호구 및 생물학적 모니터링의 효과

심윤보,이병국 大韓産業醫學會 1992 대한직업환경의학회지 Vol.4 No.1

For the purpose of investigating the effect of respiratory protection with biological monitoring on the improvement of workers' health, authors selected one storage battery factory which was scheduled to move to a new modernized factory within 2 years. Maintenance free respirators were provided to all of the workers at the beginning of this study for the respiratory protection. Zinc protoporphyrin (ZPP) in whole blood was chosen as an index of biological monitoring and was measured regularly once a month throughout 1 year-study period. Blood lead (PBB), δ -aminolevulinic acid in urine (ALAU), hemoglobin, hematocrit, lead related symtoms and other personal data were also checked twice at the beginning and the end of this study, respectively. Fourteen questionnaires of lead absorption were completed by each worker twice at the beginning and the end of this study, respectively. Out of 214 workers, authors analysed the data of 147 workers who stayed during the study period from the beginning to the end of this study, and the results obtained were as follows : 1. The percentages of workers whose blood lead was over 60 ug/dl and blood ZPP over 150 ug/dl which were the criteria for the diagnosis of lead poisoning in Safety and Health Act of Korea decreased from 237% and 23.0% at the beginning of the study to 1.3% and 1.9% at the end of the study, respectively. 2. There were significant differences of mean value in blood lead, ALAU, ZPP and overall prevalence rate of lead related symptoms between the beginning and the end of the study, but there were no differences of mean value in hemoglobin and hemotocrit between two periods. 3. While the most frequent three symptoms in male workers were "feeling tired generally", "loss of weight" and "numbness of fingers, hands or feet" in order, those in female workers were "numbness of fingers, hands or feet", "feeling tired generally" and "loss of weight" in order of frequency. 4. The prevalences of two symptoms ("cann't sleep well" and "loss of weight") increased significantly by the increase of blood lead of workers. 5. The prevalences of six symptoms ("anorexia", "numbness of fingers. hands or feet", "weakness of fingers, hands or feet" "joint pain" and "feeling tired generally") increased significantly by the increase of blood ZPP of workers. 6. The variable which showed highest correlation with overall prevalence of lead related symptoms was blood ZPP and there was also significant correlation between symptom prevalence and blood lead and ALAU. 7. There were negative correlations between symptom prevalence and age and work duration. As the age and the work duration of workers increased the symptom prevalences were decreased accordingly. 8. Stepwise multiple regression analysis, using overall symptom prevalence as a dependent variable and blood lead. ALAU, hemoglobin. hematocrit, work duration. age, sex as independent variables, indicated that only blood ZPP and age were significantly contributed to the dependent variable. With above results it was confirmed that respiratory protection with biological monitoring using blood ZPP could improve workers' health by reducing the degree of lead exposure, and blood ZPP was found to be the most relevant index to lead related symptoms.

Glassy Carbon 전극에서의 벗김 전압-전류법을 이용한 2-Amino-1-cyclopentene-1-dithiocarboxylate의 분석과 전극 반응 메카니즘의 연구

沈允輔,朴德洙,崔星洛,元美淑 부산대학교 기초과학연구소 1988 부산대학교 기초과학연구소 연구논문집 Vol.8 No.-

많은 금속이온들에 대한 킬레이트시약으로 알려진2-amino-1-cyclopentene-1-dithiocarbox-ylate(acdc) 음이온의 전기화학적 거동을 직류 폴라로 그래피, 순환 전압전류법 및 양극 벗김 전압 전류법을 사용하여 수용액 및 아세톤 용액에서 각각 조사하였다. 수용액에서 glassy carbon전극을 사용하였을 때 +0.25V vs. SCE에서 1전자 산화반응을 거쳐 acdc의 이합체가 생성됨을 확인하였으며 이 이 합체는 glassy carbon 전극 표면에 석출되어 흡착이 일어남을 알 수 있었다. 이 흡착된 이합체가 +0.80V에서 2전자 산화반응을 거쳐 S가 한원자 유리된 고리를 형성하는 반응이 일어 남을 알았다. 이러한 이합체 생성반응을 이용하여 이 화합물의 흡착성 양극 벗김 전압전류법에 의한 분석법을 조사하였다. 직류폴라로그래피을 사용한 벗김 전압-전류법에 의해 검량선을 얻은 결과 3 ×10^(-5)M∼10^(-6)M 사이에서 좋은 직선상을 얻을 수 있었으며 확산전류를 사용한 분석법에 비해 약100배 가량 분석감도가 증진됨을 알 수 있었다. 이때 검출한계는 2.5 ∼10^-7)M이었으며 5 ×10^(-6)M에서의 상대 표준편차는 ±4.1%이었다. The electrochemical behavior of 2-Amino-1-cyclopentene-1-dithiocarboxylate(acdc) was investigated by the use of polarography, cyclic voltammetry and cathodic stripping voltammetry at glassy carbon electrode. In this study, it was found that the dimer of the acdc was deposited on the glassy carbon electrode via one-electron oxidation process at ±0.25V vs.SCE. The ring formation between two dithio group occurs along with the elimination of one sulfur atom. The elimination of sulfur atom occurs via two electron oxidation process at ±0.8V vs.SCE. The most sensitive cathodic stripping peak due to the formation of the dimer was observed at -0.85V vs. SCE. The peak relationship between current and concentration was fairly linear in the range of 3 ×10^(-5)∼1.0 ×10^(6)M. The preconcentration procedure enhanced the sensitivity about 100 times for the analysis of acdc using diffusion current. Detection limit was found to be 2.5 ×10^(-7)M and relative standard deviation was ±4.1% at 5.0 ×10^(-6)M DC polarography.

옥소바나듐(Ⅳ)-카르복실레이트 착물의 자기적 및 적외선 분광학적 성질

沈允輔,崔星洛,朴鍾烈 부산대학교 물성연구소 1986 물성연구소연구논문집 Vol.6 No.-

카르복실기를 갖는 리간드들과 옥소바나듐(Ⅳ)사이에 형성되는 착화합물(VO(picn)₂,VO(htmq)₂, VO(quin)₂ 및 VO(pyra)₂ㆍ2H₂O)들을 합성하여 그들의 IR 및 자기적 특성을 조사하였다. 이들의 자화율 값과 V=O결합의 신축진동수 값으로부터 VO(picn)₂및 VO(htmq)₂ 착화합물에 있어 강한 분자간 상호작용이 존재하는 것으로 추정하였다. IR데이타를 그거로할 때 4개의 옥소바나듐(Ⅳ) 착물들에 있어 카르복실기는 모두 한자리 배위자로서 작용함을 알 수 있었다. 자유리간드와 옥소바나듐 착물의 IR스펙트럼을 비교 검토함으로써 중심원자와 리간드주게원자들로 구성되는 배위권에서의 기본진동 형태를 분류하고 착물 형성후 나타난 새로운 IR흡수띠들을 분류한 진동 형태와 대응시켰다. Four oxovandium(Ⅳ) complexes with carboxylate ligands, VO(picn)₂, VO(htmq)₂, VO(quin)₂ and VO(pyra)₂ㆍ2H₂O have been prepared and studied. Infrared spectral data coupled with the results of magnetic susceptivility study strongly suggest that there are relltively strong intermolecular interactions in VO(picn)₂ and VO(htmq)₂. In all oxobanadium(Ⅳ) complexes studied in this work, the carboxylate ligand coordinates to the metal ion in the unidentated fashion. Some fundamental vibration modes of oxovanadium(Ⅳ) complexex were empirically assigned from the difference in the spectrum of metal complex with free ligand.

점토광물을 이용한 산소환원의 전기화학적 촉매성에 관한 연구

오성훈,황진연,심윤보,이효민,윤지해 한국광물학회 2005 광물과 암석 (J.Miner.Soc.Korea) Vol.18 No.1

몇 가지 점토광물과 메틸바이올로젠 또는 메틸렌블루를 함유시킨 점토수식전극을 이용하여 산소환원에 대한 전기화학적 촉매성을 검토하였다. 점토광물로는 Na-몬모릴로나이트, Ca-몬모릴로나이트, 카오리나이트를 사용하였다. 점토수식전극은 유리탄소전극 표면에 점토 현탁액을 입히고 메틸바이올로젠을 흡착시킨 것을 사용하였으며, 전기화학적 산소환원의 정도는 순환 전압전류(cyclic voltammetry)으로 측정하였다. 실험결과, 다른 점토시료에 비하여 Na-몬모릴로나이트가 메틸바이올로젠의 흡착효과가 가장 크게 나타났고, 메틸바이올로젠을 흡착시킨 점토수식전극이 산소환원에 대한 촉매성이 월등히 우수하였다. 즉 촉매산소환원 피크가 242.6 ㎷ 만큼 +방향으로 이동하였다. 메틸바이올로젠을 흡착시킨 점토수식전극이 메틸렌블루를 흡착시킨 점토수식전극보다 산소환원에 대한 촉매성이 더 높게 나타났다. Ca-몬모릴로나이트의 경우는 변화가 없었으나 Na-몬모릴로나이트의 구조는 메틸바이올로젠의 흡착으로 변화되었다. 메틸바이올로젠- Na-몬모릴로나이트 점토수식전극을 현탁액의 점토 농도가 약 0.87 g/10 mL이고, 메틸바이올로젠의 수용액의 농도가 대략 2.5 mM일 때 산소환원 촉매 효과가 가장 탁월하였다. 지지전해질의 pH에 따른 점토수식 전극의 산소 환원 촉매성은 중성의 pH 범위(6.3과 8.3)에서보다 산성인 pH 3.7과 알칼리성인 pH12.7에서 월등히 크게 나타났다. The electrocatalytic reduction of O₂ was investigated with methyl viologen and methylene blue incorporated clay-modified electrodes. Clay suspensions were prepared with Namontmorillonite, Ca-montmorillonite, and kaolinite. The methyl viologen-clay modified electrodes were made by coating clay suspensions adsorbing methyl viologen on a glassy carbon electrode. Cyclic voltammetry were performed in aqueous media to investigate the electrocatalytic property of the modified electrode in reducing O₂. A Na-montmorillonite modified electrode showed the greatest adsorption capacity for methyl viologen. The modified electrode made of Na-montmorillonite suspension of 0.87 g/l0 mL and a 2.5 mM of methyl viologen solution showed the most effective electrocatalytic property, where the catalytic reduction potential was shifted by 242.6 ㎷ toward the positive potential. The electrocatalytic ability was more significant in acidic (pH=3.7) and alkaline (pH=12.7) media than the neutral pH range (6.3~8.3). The methyl viologen-Na-montmorillonite modified electrode had the good reproducibility and maintain the electrocatalytic property over 20 times reuse.

2-Amino-1-cyclopentene-1-dithiocarboxylate 유도체들의 전극 반응메카니즘

金映辰,金祐聖,沈允補,崔星洛 부산대학교 기초과학연구소 1990 부산대학교 기초과학연구소 연구논문집 Vol.10 No.-

N-methyl-2-amino-1-cyclopentene-1-dithiocarboxylic acid(N-CH₃acdc)와 2-amino-1-cyclopentene-1-dithiomethyl ester(S-Ch₃acdc)의 전기화학적 거동을 직류롤라로그래피, 순환전압전류법 및 조절전위 전기량법을 사용하여 dimethylformamide(DMF) 용매 중에서 조사하였다. N-CHacdc의 이합체는 +0.98V에서 2전자 산화반응을 거쳐 s가 한 원자 유리된 다섯원자 고리를 형성하는 전극반응이 일어남을 알았다. 그러나, S-CH₃acdc의 경우에 있어서는 N-CH₃acdc 화합물과는 달리 다섯원자 고리를 형성하지 않았으므로 N-CH₃acdc와는 다른 전극반응을 일으킴을 확인하였다. The electrochemical behavior of N-methyl-2-amino-1-cyclopotent-1-dithiocarboxylic acid (N-CH₃acdc) and 2-amino-1-cyclooptent-1-dithiomethyl ester (S-CH₃acdc) in DMF have been investigated by the use of polyargraphy, cyclic voltammetry and colometry. The dimer of N-CH₃acdc is further oxidized at +0.98 V via 2-electron process to produce free sulfur atom and cyclization product. The ring formation between two dithio group occurs along with the elimination of one sulfur atom. The elimination of sulfur atom occurs via two electron oxidation process at +0.98V vs. Ag/AgCl electrrode. However, the cyclization does not occur in the S-CH₃acdc.

Inprared Absorption Spectra of Oxovanadium(Ⅳ) Chelates

Shim, Yoon Bo,Choi, Sung Nak,Park chong yul 부산대학교 1983 자연과학논문집 Vol.36 No.-

적외선 분광법(2000cm^(-1)~200cm^(-1)) 범위를 사용하여 VO(L)_(2)형 옥소바나디움(Ⅳ) 착화합물의 결합특성을 조사하였다. 자유리간드와 옥소바나디움착물의 적외선 분광 스펙트럼을 비교 검토하므로서 중심 원자가 리간드주게원자들로 구성되는 배위권에서의 기본진동형태를 분류하고 1000cm^(-1)~200cm^(-1) 범위에서 착물 형성 후 나타난 새로운 적외선 흡수띠들을 분류한 진동 형태와 대응시켰다. 또한 착물 형성 시 진동 에너지의 변화를 나타내는 키르복실기(-COOH), 하이드록실기(-OH) 및 기타 결합의 특성 진동수를 찾아내었다.

Polarographic Behavior of Oxovanadium (IV) Complex of Mercaptopyridine N-Oxide

Shim, Yoon-Bo,Choi, Sung-Nak Korean Chemical Society 1987 Bulletin of the Korean Chemical Society Vol.8 No.4

The redox properties of 2-mercaptopyridine N-oxide (mpno) and its oxovanadium complex, $VO (mpno)_2$ have been studied by the use of polarography and cyclic voltammetry. The radical anion of mpno is generated in acetone and is adsorbed to the electrode to form an adsorption wave at -0.21 V vs Ag/AgCl electrode. The normal wave appeared at -0.50 V is attributed to the formation of radical anion. The $VO (mpno)_2$ exhibits one oxidation wave at +0.57 V, and two reduction waves at -1.07 V and -1.76 V vs. Ag/AgCl electrode; the oxidation is fully reversible one-electron process ($VO (mpno)_2\;{\leftrightarrow}\;VO(mpno)_2^+ + e).$ The reduction wave at -1.07 V is quasireversible and is arised from the formation of $VO (mpno)_2^-.$ The second reduction wave at -1.76 V is irreversible and this reduction process consists of two one-electron steps. The sulfur containing ligands seem to enhance the stability of lower oxidation state of vanadium while the oxygen or nitrogen donor of the ligands stabilize the higher oxidation state of vanadium when comparisons are made among several oxovanadium complexes.

Modulation of the Specific Interaction of Cardiolipin with Cytochrome c by Zwitterionic Phospholipids in Binary Mixed Bilayers : A2H - and 31P - NMR Study

Shim, Yoon Bo,Park, Jang Su,Kang, Shin Won,Kim, Andre,Jeong, In Chul 한국생화학회 1978 Vol. No.

The interaction of cytochrome c with binary phospholipid mixtxres was investigated by solid-state ²H- and ^(31)P-NMR. To examine the effect of the interaction on the glycerol backbones, the glycerol moieties of phosphaddylcholine (PC), phosphafidylethanolamine (PE), phosphatidylglycerol (PG), and cardiolipin (CL) were specifically deuterated. On the binding of cytochrome c to the binary mixed bilayers, no changes in the quadrupole splittings of each of the components were observed for the PGPG, PE/CL and PFJ PG 6posomes. In contrast, the splittings of CL decreased on binding of protein to the PGCL liposomes, although those of PC did not change at all. This showed that cytochrome c specifically interacts with CL in PGCL bilayers, and penetrates into the lipid bilayer to some extent so as to perturb the dynamic structure of the glycerol backbone. This is distinctly different from the mode of interaction of cytochrome c with other binary mixed bilayers. In the ^(31)P-NMR spectra, line broadening and a decrease of the chemical shift anisotropy were observed on the binding of cytochrome c for all binary mixed bilayers that were examined. These changes were more significant for the PG CL bilayers. Furthermore, the line broadening is more significant for PC than for CL in PGCL bilayers. Therefore, it can be concluded that with the polar head groups, not only CL but also PC are involved in the interaction with cytochrome c.

Determination of Copper, Lead and Cadmium in Natural Water by Anodic Stripping Voltammetry with Solvent Extraction

Shim, Yoon Bo,Won, Mi Sook,Jeong, Gi ho 부산대학교 1981 자연과학논문집 Vol.32 No.-

Copper, lead and cadmium in natural water determined by anodic stripping voltammetry with solvent extraction. A glassy carbon electrode was also used. 50㎖ of sample solution was extracted three times with 10㎖ of 0.01% dithizone-chloroform. When the chloroform layer was back extracted with 10㎖ 0.1 N HCl containing excess mercuric ions, the free metal ions come into aqueous layer. 5㎖ of this aqueous solution contained residual mercuric ion was not stripped into chloroform layer but copper, lead and cadmium ions were back extracted in aqueous layer. And then the anodic stripping curve was recorded. The concentration of copper, lead can be determined up to 4 ppb and that of cadmium up to 6ppb within an error of 10%.

Stable Isotope Labeled Cytochrome c3 from Desulfovibrio vulgaris on a Defined Medium as Sole Nitrogen Source

Shim, Yoon Bo,Park, Jang Su,Kang, Shin Won,Kim, Andre 생화학분자생물학회 2001 BMB Reports Vol.33 No.6

To obtain Cytochrome c₃ labeled with a stable isotope, the conditions of cultivation and the composition of medium for DvMF were examined. The growth of DvMF was steady and reproducible under purging with N₂ and under pH control. DvMF was able to go on a defined medium without natural products. The composition of the medium containing a small amount of NH,CI as sole nitrogen source was established. Then, uniformly ^(15)N-labeled Cytochrome c₃ was obtained during the culture of DvMF in a defined medium with ^(15)NH₄Cl; it was confirmed by ¹H-^(15)N HMQC.