본태성 고혈압에서 염산에포니디핀 (NZ-105, 핀테20정�)의 유효성 및 안전성 : 제3상 다기관 임상시험 The phaseⅢ multicenter open trial

배종화,손일석,강흥선,조정휘,강재헌,김성원,이선영,양윤준,김영식,선우성 대한간질학회 2003 대한간질학회지 Vol.9 No.1

톨라신 농도에 따른 세포독성 기작과 Ni2+과 Zn2+ 에 의한 저해

윤영배 · 한지혜 · 김영기 忠北大學校 農業科學硏究所 2018 農業科學硏究 Vol.34 No.2

본문 참고

RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단

배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.

일부 초등학생의 비만도에 따른 칼슘과 철의 섭취상태와 혈청 농도에 관한 연구

이화성,김은영,배윤정,승정자 숙명여자대학교 건강·생활과학연구소 2006 生活科學硏究誌 Vol.- No.22

The purpose of this study was to evaluate the nutrition status of calcium and iron in 116 elementary school students with different obesity index. Subjects were assigned to under weight, normal weight or over weight group according to the their obesity index. Their nutritional status, serum calcium and iron levels were evaluated based on 24-recall dietary recalls, anthropometric measurements and blood analysis. The mean height, weight, obesity index and blood pressure of total subjects were 150.4 cm, 45.5 kg, 5.8% and 118.2/76.8 mmHg. The mean % of body fat, SBP and WHR were higher while LBM and TBW were lower in over weight subject than those of other group. The iron intake of overweight was lower than that of underweight and normal subjects(p<0.05) and average calcium intakes were only 50% and 60% respectively of the RDA for Korean. Serum calcium and iron levels of a three groups were within the normal range. There were no significant differences in serum calcium and iron concentrations among three groups, however those of underweight group were a slightly lower than those of other two groups. There were significantly negative correlations between iron of intake and weight, obesity index, systolic pressure and body fat percent. Systolic blood pressure was negatively correlated to the intakes of energy, carbohydrate and protein. Energy intake was also negatively correlated to serum iron. To summarize the results, calcium and iron intakes should be supplied to elementary school students, especially those who were overweight. These indicated that overweight group had improper mineral nutrition status. Therefore, nutrition education and profound studies from many different focus for overweight groups are highly required.

Characterization of Phage-Resistant Strains Derived from Pseudomonas tolaasii 6264, which Causes Brown Blotch Disease

( Yeong-bae Yun ),( Ji-hye Han ),( Young-kee Kim ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12

Pseudomonas tolaasii 6264 is a representative strain that causes bacterial blotch disease on the cultivated oyster mushroom, Pleurotus ostreatus. Bacteriophages are able to sterilize the pathogenic P. tolaasii strains, and therefore, they can be applied in creating disease-free mushroom cultivation farms, through a method known as “phage therapy”. For successful phage therapy, the characterization of phage-resistant strains is necessary, since they are frequently induced from the original pathogenic bacteria in the presence of phages. When 10 different phages were incubated with P. tolaasii 6264, their corresponding phage-resistant strains were obtained. In this study, changes in pathogenic, genetic, and biochemical characteristics as well as the acquired phage resistance of these strains were investigated. In the phylogenetic analyses, all phage-resistant strains were identical to the original parent strain based on the sequence comparison of 16S rRNA genes. When various phage-resistant strains were examined by three different methods, pitting test, white line test, and hemolytic activity, they were divided into three groups: strains showing all positive results in three tests, two positive in the first two tests, and all negative. Nevertheless, all phage-resistant strains showed that their pathogenic activities were reduced or completely lost.

Biological Characterization of Various Strains of Pseudomonas tolaasii that Causes Brown Blotch Disease

Yun, Yeong-Bae,Park, Seong-Wan,Cha, Jae-Soon,Kim, Young-Kee The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.1

Brown blotch disease of cultivated mushrooms is caused by Pseudomonas tolaasii, which secretes the bacterial toxin, tolaasin. Tolaasin is a peptide toxin that causes pore formation in the plasma membrane of mushroom cells. Forty-two strains of pathogenic bacteria causing brown blotch or similar diseases were isolated from mushrooms showing disease symptoms. To characterize these bacteria, the genes of 16S rRNA were sequenced and analyzed. Thirty-three strains were identified as five different species of Pseudomonas. Of these, 23 were identified as P. tolaasii and named as P1-type pathogens. Because the strains identified as P. tolaasii were major pathogens that cause the brown blotch disease, phylogenetic analyses of these pathogens were conducted by the neighbor-joining method. These strains comprised three phylogenetic subtypes, $P1{\alpha}$ (6 strains), $P1{\beta}$ (16 strains), and $P1{\gamma}$ (1 strain). Biological characterizations of the isolated bacteria were performed and confirmed that all three subtypes were able to cause the disease by forming blotches on the surface of the mushroom tissue. However, hemolytic activities were observed in the $P1{\alpha}$ and $P1{\gamma}$ strains, but not in the $P1{\beta}$ strains. These results imply that remarkable diversity exists among the various strains of P. tolaasii, each strain showing distinct biological characters.

Biological Characterization of Various Strains of Pseudomonas tolaasii that Causes Brown Blotch Disease

Yeong-Bae Yun,Seong-Wan Park,차재순,김영기 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.1

Brown blotch disease of cultivated mushrooms is caused by Pseudomonas tolaasii, which secretes the bacterial toxin,tolaasin. Tolaasin is a peptide toxin that causes pore formation in the plasma membrane of mushroom cells. Forty-two strains of pathogenic bacteria causing brown blotch or similar diseases were isolated from mushrooms showing disease symptoms. To characterize these bacteria, the genes of 16S rRNA were sequenced and analyzed. Thirty-three strains were identified as five different species of Pseudomonas. Of these, 23 were identified as P. tolaasii and named as P1-type pathogens. Because the strains identified as P. tolaasii were major pathogens that cause the brown blotch disease, phylogenetic analyses of these pathogens were conducted by the neighbor-joining method. These strains comprised three phylogenetic subtypes, P1α (6 strains), P1β (16 strains), and P1γ(1 strain). Biological characterizations of the isolated bacteria were performed and confirmed that all three subtypes were able to cause the disease by forming blotches on the surface of the mushroom tissue. However, hemolytic activities were observed in the P1α and P1γ strains, but not in the P1β strains. These results imply that remarkable diversity exists among the various strains of P. tolaasii, each strain showing distinct biological characters.

Changes in growth and active components of Angelica gigas Nakai in different cultivation environments

Yeong-Bae Yun,Dae-Hui Jeong,Hae-Yun Kwon,Yurry Um 한국응용생명화학회 2024 Journal of Applied Biological Chemistry (J. Appl. Vol.67 No.-

Angelica gigas Nakai belongs to the Unbelliferae family and was a representative medicinal plant with antiinflammatory, antioxidant and immunoenhancing activities. Recently, changes in the atmospheric environment, such as temperature, CO2 concentration and solar radiation, have continuously resulted in the reduction of cultivation area and production amount of A. gigas. They could affect the synthesis of secondary metabolites including nodakenin, decursin and decursinol angelate. This study aimed to investigate the atmospheric factors and the growth and content of active compounds of A. gigas. Air temperature and soil temperature were lowest in Jeongseon (northern region). Solar radiation was highest in Bonghwa (central region). Relative humidity was the lowest in Jeongseon from January to June, and it was the lowest in Bonghwa after July. At the Jeongseon experimental site, most of the growth characteristics of A. gigas were poor. The growth characteristics of A. gigas in Chungju and Bonghwa did not show any significant difference. The contents of active compounds were significantly highest in A. gigas collected from Chungju, but were significantly low in Jeongseon. Relative humidity showed a significant positive correlation with the content of the active compounds except for nodakenin. Decursin and decursinol angelate were significantly negatively correlated with solar radiation and soil moisture content, respectively. Most of growth characteristics of A. gigas, except for root length, showed a significant positive correlation with nodakenin content. The results of this study can be used as data for predicting the location of A. gigas cultivation according to the growth and active compounds of A. gigas in different cultivation regions.

자궁경부 원추절제술과 관련된 자궁적출술에서 조직병리와 절단경계면의 침범여부에 기초한 잔류병소의 예측에 관한 연구

윤영민 ( Yun Yeong Min ),이주열 ( Lee Ju Yeol ),전현아 ( Jeon Hyeon A ),김홍배 ( Kim Hong Bae ),이근영 ( Lee Geun Yeong ),강성원 ( Kang Seong Won ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.5

목적 : 원추절제술 후 남은 자궁경부에서 잔류병소의 예측인자로 원추절제 경계면의 침범상태와 자궁경부 종양의 심도를 평가하고자 하였다. 연구 방법 : 1996년 1월부터 2002년 6월까지 원추절제술 후 자궁적출술을 시행 받은 146명의 환자들을 대상으로 후향적 연구를 시행하였다. 잔류병소의 예측인자로 환자의 나이와 출산력, 원추절제 검체의 조직병리정도와 절단면 침범상태가 분석되었다. 결과 : 절단면 양성의 이환율 LSIL 0%, HSIL 15%, CC-Ⅰa1 43.3%, CC-Ⅰa2 85.7%였다. 원추절제술 후 자궁적출술 검체에서 잔류병소 발생율은 LSIL 0%, HSIL 23.3%, CC-Ⅰa1 35.5%, CC-Ⅰa2 100%였다. 잔류병소는 양성 절단면인 경우에서 음성 절단면인 경우에서보다 유의하게 높은 빈도를 보였다. 양성 절단면의 경우 잔류병소에 대한 양성 예측율은 LSIL 0%, HSIL 22.2%, CC-Ⅰa1 57.6%, CC-Ⅰa2 100%였다. 양성 절단면의 경우 8.3%에서만이 자궁적출술에서 더 진행된 병변을 보였다. 음성 절담녀의 경우 잔류병소에 대한 음성 예측율은 LSIL 100%, HSIL 70.5%, CC-Ⅰa1 81.4%, CC-Ⅰa2 0%였다. 결론 : 원추절제술 양성 절단면과 잔류병소의 발생율은 자궁경부 종양의 심도에 따라 증가한다. 양성절단면인 경우 음성 절단면인 경우보다 잔류병소와의 높은 상관관계를 보였으나 음성절단면이 잔류병소가 존재하지 않는다는 것을 확신하지는 못한다. 또한, 양성 절단면이 항상 잔류병소의 존재와 지속성을 지적하지는 않는다. 그러므로 원추절제술 후 자궁적출술은 양성 절단면을 가진 고등급 자궁경부 종양이나 자궁경부암인 경우, 동시에 다른 자궁의 병변이 있는 경우나 지속적 추적관찰이 불가능한 경우의 환자에게 고려되어져야 한다. Objective : The objective of this study is to evaluate predictive factors for residual lesion after conization in patients with cervical neoplasia. Methods : we performed retrospective study (between January 1996 and June 2002) and reviewed 146 patients who had undergone cervical conization followed by subsequent hysterectomy. The age and the parity of patients, the histopathology and the status of resection margins of cone specimens were analysed as predictive values of residual lesions. Results : The prevalence rate of positive cone margins were 0%, 15%, 43.4%, and 85.7% respectively in patients with low-grade squamous intraepithelial lesion (LSIL) (n=3), high-grade squamous intraepithelial lesion (HSIL) (n=60), Cervical cancer stage Ⅰa1 (CC-Ⅰa1) (n=76) and Cervical cancer stage Ⅰa2 (CC-Ⅰa2) (n=7). The prevalence rates of positive residual lesions in post-cone hysterectomy specimens were 0%, 23.3%, 35.5%, and 100% respectively in those with LSIL, HSIL, CC-Ⅰa1, and CC-Ⅰa2. Residual lesions were significantly more frequently found in patients with positive cone margin (56.3%) than in those with negative margin (21.4%) (P value=0.000026). Positive predictive values of margin status for the presence of residual lesions were 0% (LSIL), 22.2% (HSIL), 57.6% (CC-Ⅰa1) and 100% (CC-Ⅰa2). Negative predictive values of margin status for the absence of residual lesions were 100% (LSIL), 76.5% (HSIL), 81.4% (CC-Ⅰa1) and 0% (CC-Ⅰa2). Only 8.3% (4/48) of patients with positive cone margin had more advanced residual lesions. 9.2% (9/98) of patients with negative margins had invasive residual lesion. Conclusion : The prevalence rate of positive cone margin and residual lesion increased with high severity of cervical neoplasia. Patients with positive cone margin had significantly higher chances of having residual lesion than those with negative margin. Free cone margin does not ensure the absence of residual lesion in the remaining cervix. Positive cone margin does not invariably indicate the presence and persistence of more severe residual lesion. Subsequent hysterectomy may be reserved for the patients with invasive cone pathology, concomitant morbid uterine condition, or not reliable for continuous follow-up.

톨라신 저해 물질을 이용한 갈반병의 억제

윤영배 ( Yeong-bae Yun ),김민희 ( Min-hee Kim ),한지혜 ( Ji-hye Han ),김영기 ( Young-kee Kim ) 한국응용생명화학회 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.2

톨라신은 1.9 kDa의 펩티드 독소로서 Pseudomonas tolaasii에 의해 생성되며, 재배중 느타리버섯에 갈반병을 일으킨다. 톨라신은 막에 pore를 형성하여 세포 구조를 파괴하고, 버섯 재배의 생산성을 심하게 감소시킨다. 톨라신에 의한 세포독성의 작용 기작은 완전히 밝혀지지 않았지만, 분자다중화에 의해 세포막에 채널구조 형성으로 이루어진다. 그러므로, 톨라신과 작용하는 식품첨가물 중에 톨라신의 다중화결합을 통한 세포막 pore형성을 저해하는 물질이 있을 것이다. 본 연구에서는, 다양한 물질들이 톨라신의 활성을 저해함을 확인하고, 이들을 톨라신저해물질(TIF)이라 명명하였다. 대부분의 톨라신 저해물질들은 식품가공과정에 쓰이는 유화제였다. 다양한 종류의 저해물질 중에 지방산과 에스터 결합한 polyglycerol과 지방산과 에스터 결합한 sucrose 화합물이 10^-4-10^-5 M 농도범위에서 톨라신의 세포독성을 효과적으로 저해하였다. 이러한 저해물질들은 균상재배하는 느타리버섯에서 갈반병의 발생을 성공적으로 억제하였다. Tolaasin, a 1.9 kDa peptide toxin, is produced by Pseudomonas tolaasii and causes the brown blotch disease of cultivated oyster mushroom. It forms pores on the membrane and thus destroys cellular membrane structure, seriously reducing the productivity of mushroom cultivation. The mechanism of tolaasin-induced cytotoxicity is not known in detail. However, it has been reported to form a pore structure in the cytoplasmic membrane through the molecular multimerization. Therefore, food additives which can interact with tolaasin molecules may inhibit the pore formation by hydrophobic interactions with tolaasin molecules. In this study, various food additive materials have been identified as inhibitors of the tolaasin activity and named tolaasin-inhibitory factors (TIF). Most of TIFs are emulsifying agents for food processing procedures. Among various TIFs, polyglycerol and sucrose esters of fatty acids blocked effectively the cytotoxicity of tolaasins at the concentrations 10^-4-10^-5 M. These TIFs also successfully suppressed the blotch disease development in the shelf cultivation of oyster mushroom.