Poster Session:PS 1146 ; Gastroenterology (Pancreatobilliary) : Tumor Forming Chronic Pancreatitis Masquerading as Pancreatic Adenocarcinoma

( Myung Woo Choi ),( Jong Hwa Lee ),( Sung Sik Oh ),( Mi Rim Choi ),( Hyun Ju Yang ),( Moon Sik Park ),( Jae Un Lee ),( Shang Hoon Han ),( Young Jae Lee ),( Yong Keun Cho ),( Ji Woong Kim ),( Ji Woong 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Introduction: There are various tumorous lesions in pancreas, but it is diffi cult to differenciate these lesions because of the anatomical and clinical characteristics. Through endoscopic-ultrasound(EUS)-guided biopsy, the effort for increasing diagnostic rate is still ongoing. However, Sometimes it is diffi cult to get enough tissue through EUS and to get accurate diagnostic imaging of tumorous lesion. In that case, the defi nitive diagnosis is only obtained after surgical intervention. Authors report one case, which was diagnosed as the pancreatic cancer before the operation and had a pancreaticoduodenectomy, but the lesion was proved to be tumor forming chronic pancreatitis on histological examination of a frozen-section. Case: A 65-years-old male patient presented with indigestion for about 1week and he was found to have a tumorous lesion in pancreas by abdominal-ultrasonography at local. He was a chronic drinker who consumed 100g of alcohol everyday. On initial laboratory evaluation, PT-1. 02, AST/ALT-22/12U/L, ALP-342IU/L, T-bilirubin-0. 2mg/ dL, Amylase-74U/L, lipase-160U/L, CA19-9-9. 13U/mL, CEA-6. 1ng/mL. In Contrast-enhanced abdominal-CT, 6. 4x5. 0x4. 0cm mass was found in the pancreas head, which was low density and little more enhanced than normal pancreas and the boundary was relatively clear. EUS-guided Procore 25G-needle biopsy and Cytology was done, but only normal pancreas tissues were found. However there was FDG uptake (SUVmax- 3. 79) in the head of pancreas on PET-CT. So we diagnosed this case as pancreas- cancer in T3N1 and he underwent pancreaticoduodenectomy. After the operation, fi nal histological diagnosis was mass forming chronic pancreatitis. Conclusion: Chronic pancreatitis can present with a fi brotic mass. If there is a risk factor like chronic alcohol intake in medical history taking and CA 19-9 is normal, it could be the chronic pancreatitis which has a possibility of forming mass rather than pancreatic cancer. In conclusion, it needs more effort of imaging examination and tissue confi rmation for accurate diagnosis.

Effect of Genistein and Daidzein on Glucose Uptake in Isolated Rat Adipocytes; Comparison with Respective Glycones

Choi, Myung-Sook,Jung, Un-Ju,Kim, Myung-Joo,Kim, Jong-Yeon,Park, So-Young,Jang, Joo-Yeum,Lee, Mi-Kyung The Korean Society of Food Science and Nutrition 2005 Preventive Nutrition and Food Science Vol.10 No.1

Soy and soy foods are a rich source of isoflavones, which possess several biological activities. The effect of soy isoflavones, genistin and diadzin and their respective aglycones, on glucose uptake in adipocytes isolated from normal or high-fat fed rats was examined. As expected, insulin stimulated glucose uptake in a concentration-dependent manner. However, genistin and daidzin and their aglycones inhibited glucose uptake in a concentration-dependent (25-100μM) manner. In a time-course response, the aglycones significantly inhibited glucose uptake throughout 3 hr (after 30, 60, 120, 180 min), whereas the glycones only significantly inhibited the glucose uptake after 120 min and 180 min in the isolated rat adipocytes. Thus, the glucosides of genistein and daidzein, i.e. genistin and daidzin, were much less effective in inhibiting glucose uptake than their aglycones. In addition, genistin and daidzin did not significantly affect the insulin-stimulated glucose uptake, whereas genistein and daidzein did significantly inhibited glucose uptake compared to the vehicle control group by 47.5% and 24.8%, respectively (p < 0.05). The isoflavones also significantly inhibited glucose uptake in adipocytes isolated from rats fed a high-fat diet (50% of total calorie intake) when compared to the vehicle control. Finally, the isoflavones were found to enhance lipolysis in adipocytes isolated from high-fat fed rats, where the glycerol released by the aglycones was also higher than that released by the glycones. The current results showed that the inhibitory effect of daidzein on glucose uptake was very similar to that of genistein. The aglycones were more potent in inhibiting the uptake of glucose and a more potent stimulator of lypolysis than the glycones in adipocytes isolated from high-fat fed rats.

Purification and Characterization of Protein Phosphatase 2C from Rat Liver

Choi, Myung Un,Hwang, In Seong,Oh, Joung Sook 생화학분자생물학회 2000 BMB Reports Vol.30 No.3

Protein phosphatase 2C (PP2C) is one of the four major serine/threonine phosphatases which is dependent on Mg^(2+) for its activity. PP2C was purified from rat liver cytosol and its characteristics were investigated. The substrate employed for routine assay was [32P]casein phosphorylated by PKA. The purification process involved DEAE chromatography, ammonium sulfate fractionation, phenyl sepharose chromatography, sephacryl S-200 gel filtration, and histone agarose chromatography. The SDS-PAGE of PP2C showed one major single protein band at a position corresponding to a molecular mass of 43 kd and the purification fold was 637. The enzyme showed a pH optimum of 8 and KM value was 1.9 μM. However, when the substrate was changed to [^(32)P]histone, the pH optimum was shifted to 7 and K_M value was 2.3 μM. Mg^(2+) was essential to the enzyme activity and okadaic acid did not exert any inhibitory effect on the enzyme. To examine residue in the active site of PP2C, effects of some protein-modifying reagents were tested.

Effect of Genistein and Daidzein on Glucose Uptake in Isolated Rat Adipocytes ; Comparison with Respective Glycones

Myung-Sook Choi,Un Ju Jung,Myung-Joo Kim,Jong-Yeon Kim,So-Young Park,Joo-Yeun Jang,Mi-Kyung Lee 한국식품영양과학회 2005 Preventive Nutrition and Food Science Vol.10 No.1

Soy and soy foods are a rich source of isoflavones, which possess several biological activities. The effect of soy isoflavones, genistin and diadzin and their respective aglycones, on glucose uptake in adipocytes isolated from normal or high-fat fed rats was examined. As expected, insulin stimulated glucose uptake in a concentration- dependent manner. However, genistin and daidzin and their aglycones inhibited glucose uptake in a concentration-dependent (25~100 μM) manner. In a time-course response, the aglycones significantly inhibited glucose uptake throughout 3 hr (after 30, 60, 120, 180 min), whereas the glycones only significantly inhibited the glucose uptake after 120 min and 180 min in the isolated rat adipocytes. Thus, the glucosides of genistein and daidzein, i.e. genistin and daidzin, were much less effective in inhibiting glucose uptake than their aglycones. In addition, genistin and daidzin did not significantly affect the insulin-stimulated glucose uptake, whereas genistein and daidzein did significantly inhibited glucose uptake compared to the vehicle control group by 47.5% and 24.8%, respectively (p<0.05). The isoflavones also significantly inhibited glucose uptake in adipocytes isolated from rats fed a high-fat diet (50% of total calorie intake) when compared to the vehicle control. Finally, the isoflavones were found to enhance lipolysis in adipocytes isolated from high-fat fed rats, where the glycerol released by the aglycones was also higher than that released by the glycones. The current results showed that the inhibitory effect of daidzein on glucose uptake was very similar to that of genistein. The aglycones were more potent in inhibiting the uptake of glucose and a more potent stimulator of lypolysis than the glycones in adipocytes isolated from high-fat fed rats.

Detection of Cytosolic Phosphatidylethanolamine N-Methyltransferase in Rat Brain

Choi,Myung-Un,Kim,Young Jun,Park,Heung Soon The Korea Science and Technology Center 1998 BMB Reports Vol.31 No.2

Phosphatidylethanolamine N-methyltransferase (PEMT) is known to be a membrane-associated protein. However, cytosolic PEMT was detected when sufficient amounts of exogenous phospholipids were added in the incubation media. The methylation of phospholipids was measured by the incorporation of the [H]-methyl group from S-adenosylmethionine and the methylated phospholipids were analyzed by thin-layer chromatography. The essence of the assay condition for the enzyme was the inclusion of 200㎍ of each substrate, phosphatidylethanolamine(PE), phosphatidyl N-monomethylethanolamine (PME) and phosphatidyl N,N-dimethylethanolamine (PDE), in the reaction mixture of 100 l. he subcellular fractionation of brain PEMT activities revealed that approximately 38.1% for PME, 39.5% for PDE, and 22.4% for PC formation was present in the cytosolic fraction. The general properties of cytosolic PEMT were characterized and compared with those of neuronal nuclei PEMT.

Du-zhong (Eucommia ulmoides Oliver) leaf extract mediates hypolipidemic action in hamsters fed a high-fat diet.

Choi, Myung-Sook,Jung, Un Ju,Kim, Hye-Jin,Do, Gyeong-Min,Jeon, Seon-Min,Kim, Myung-Joo,Lee, Mi-Kyung Institute for Advanced Research in Asian Science a 2008 The American journal of Chinese medicine Vol.36 No.1

<P>This study examined the effect of a Du-zhong (Eucommia ulmoides Oliver) leaf extract (0.175 g/100 g diet) that was supplemented with a high-fat diet (10% coconut oil, 0.2% cholesterol, wt/wt) on hyperlipidemic hamsters. Hamsters fed with Du-zhong leaf extract for 10 weeks showed a smaller size of epididymal adipocytes compared to the control group. The supplementation of the Du-zhong leaf extract significantly lowered the plasma levels of triglyceride, total cholesterol, LDL-cholesterol, non HDL-cholesterol, and free fatty acid, whereas it elevated the HDL-cholesterol/total cholesterol ratio and apolipoprotein A-I levels. The hepatic cholesterol concentration was lower in the Du-zhong group than in the control group. The plasma total cholesterol concentration was positively correlated with hepatic HMG-CoA reductase activity (r = 0.547, p < 0.05) and hepatic cholesterol concentration (r = 0.769, p < 0.001). The hepatic fatty acid synthase and HMG-CoA reductase activities were significantly lowered by a Du-zhong leaf extract supplement in high fat-fed hamsters. Hepatic fatty acid synthase activity was positively correlated with plasma fatty acid concentration (r = 0.513, p < 0.05) that was lower in the Du-zhong group. These results demonstrate that the Du-zhong leaf extract exhibits antihyperlipidemic properties by suppressing hepatic fatty acid and cholesterol biosynthesis with the simultaneous reduction of plasma and hepatic lipids in high fat-fed hamsters.</P>

쥐 뇌 Microsome 의 용해된 Cholesterol Ester Hydrolase 의 반응속도론적 경향

최명언 ( Myung Un Choi ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.4

Microsomal cholesterol ester hydrolase of rat brain has been solubilized with good yield and stability. The optimal solubilization was achieved by homogenization and high speed centrifugation of microsomes at 10 ㎎ dry weight/㎖ of Tris-HCl buffer, pH 7.6, containing 0. 75 % Triton X-100. The solubilized enzyme was more dependent on exogenous lipid for activity than the microsome-bound enzyme, but the optimal concentration of Triton X-100 was greatly reduced. Series of experiments, in with concentrations of Triton X-100, the substrate, and the solubilized enzyme were varied independently, suggested that the activation effect of the detergent depended more on the ratio of detergent to enzyme than on the ratio of detergent to substrate. The observed role of detergent is helpful to evaluate the kinetic behavior of the enzyme in the presence of detergent.

방사선 조사에 의한 쥐 조직의 포스포리파제 D의 활성 변화

최명선(Myung Sun Choi),조양자(Yang Ja Cho),최명언(Myung-Un Choi) 대한방사선종양학회 1997 Radiation Oncology Journal Vol.15 No.3

목 적 : Phospholipase D (PLD)는 phosphatidylcholine을 phosphatidic acid (PA)와 choline으로 가수 분해 시키는 효소이다. 최근 이 효소는 다른 phospholipase들과 유사하게 세포 신호전달과정에 관여하는 것으로 알려져 많은 관심의 대상이 되고 있으며, 아울러 발암과정에 관여하리라는 추측을 하게 하고 있다. 이 실험에서는 쥐를 방사선 조사하여 각 조직에서 올레산-PLD에 미치는 영향을 관찰하였다. 방 법:PLD assay를 위한 반응 혼합물에는 0.1μCi의 1,2-di[1-14C]palmitoyl phosphatidylcholine, 0.5mM phosphatidylcholine, 5mM sodium oleate, 0.2% taurodeoxycholate, 50 mM HEPES buffer(pH 6.5), 10mM CaCl2와 25mM KF 를 함께 넣어주었다. 생성된 PA는 TLC로 분리하여 그 방사능을 측정하였다. 사용된 동물은 암컷 Wistar 쥐로서 코발트 60 원격치료 기기를 이용, 조사범위를 10cm×10cm로하여 분당 선량율 2.7Gy로 방사선 조사선량 10Gy와 25Gy를 조사 하였다 결 과:PLD 활성은 폐조직에서 가장 높았으며 신장, 근육, 뇌, 비장, 골수, 흉선, 간의 순으로 나타났다. 방사선 조사결과 PLD 활성에 변동을 보인 조직은 흉선, 비장, 폐와 골수이며, 특히 흉선과 비장은 PLD의 활성이 각각 2배 이상 증가한 것으로 관찰되었다. 이와는 반대로 골수의 PLD는 30% 이상 감소한 것으로 나타났다. 한편 PLD 활성값이 가장 낮은 간은 방사선 영향을 거의 받지 않는 것처럼 보였다. 결 론:동물전신에 방사선 조사시 PLD가 가장 민감한 영향을 받는 조직은 림프양 기관과 조혈 세포인 것으로 보여 PLD가 이들 조직의 생리기능과 밀접한 관계가 있음을 암시해 주고있다. 더 나아가 방사선 긴장 (radiation stress)이 이들 조직의 세포증식내지 괴사현상연구에 중요한 수단을 제공해 줄 수 있을 것이다. Purpose:Phospholipase D (PLD) catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid (PA) and choline. Recently, PLD has been drawing much attentions and considered to be associated with cancer process since it is involved in cellular signal transduction. In this experiment, oleate-PLD activities were measured in various tissues of the living rats after whole body irradiation. Material and Methods :The reaction mixture for the PLD assay contained 0.1μCi 1,2-di[1-14C]palmitoyl phosphatidylcholine, 0.5mM phosphatidylcholine, 5mM sodium oleate, 0.2% taurodeoxycholate, 50mM HEPES buffer(pH 6.5), 10mM CaCl2, and 25mM KF. phosphatidic acid, the reaction product, was separated by TLC and its radioactivity was measured with a scintillation counter. The whole body irradiation was given to the female Wistar rats via Cobalt 60 Teletherapy with field size of 10cm×10cm and an exposure of 2.7Gy per minute to the total doses of 10Gy and 25Gy. Results:Among the tissues examined, PLD activity in lung was the highest one and was followed by kidney, skeletal muscle, brain, spleen, bone marrow, thymus, and liver. Upon irradiation, alteration of PLD activity was observed in thymus, spleen, lung, and bone marrow. Especially PLD activities of the spleen and thymus revealed the highest sensitivity toward γ-ray with more than two times amplification in their activities. In contrast, the PLD activity of bone marrow appears to be reduced to nearly 30%. Irradiation effect was hardly detected in liver which showed the lowest PLD activity. Conclusion:The PLD activities affected most sensitively by the whole-body

쥐 뇌의 Peroxidase 활성도 세포내 및 뇌 부위별 분포

최명언 ( Myung Un Choi ) 생화학분자생물학회 1982 BMB Reports Vol.15 No.4

The peroxidase activity of rat brain was determined by spectrophotometrically using o-dianisidine as substrate. Basic assay conditions were optimized in order to eliminate the pseudoperoxidase activity. The peroxidase activities were then examined in subcellular fractions as well as in six different regions of the brain. It was found that a major part of the activity was associated with particulated fractions. The specific activity of the microsomal enzyme was roughly twice as high as that of the mitochondrial. The regional distribution of the microsomal activity was quite variable. The highest activity was found in the medulla oblongata and lowest in the striatum. The possible role of the brain peroxidase was birefly assessed.

Alterations in Blood Electrolyte of Rabbits with Experimental Injection of Escherichia coli Endotoxin

Choi, Seok-Cheol,Kim, Jai-Young,Kwon, Heun-Young,Kim, Tae-Un,Hwang, Soo-Myung,Lee, Won-Jae 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.2

혈액 전해질 성분들에 대한 대장균 내독소의 영향을 조사하기 위해 토끼를 대상으로 한 동물실험을 실시하였다. 대장균 내독소(혈청형 O55: B5)를 토끼의 귀정맥을 통해 0.10mg/kg 혹은 0.50mg/kg농도로 주입한 후 3,6,12,24시간대에 채혈하여서 Ca++,Mg++, K+, CI- 농도를 측정하였다. 대조군에 비해, 내독소투여 토끼의 Ca++농도는 6시간대에 증가하였고, Mg++농도는 3,6,12시간대에, Na++과 K+는 모든 채혈시간대에, 그리고 CI- 농도는 3,12,24시간대에 각각 유의하게 높았다(p<0.05). 흥미롭게도, 고 Mg+혈증(약 4.0mg/dL)을 보인 내독소투여 토끼들은 심각한 임상징후들로 인식되는 분비물의 증가, 쇼크, 빈호흡, 경련, 혹은 설사와 같은 증세를 보였다. 본 연구의 결과들은 대장균 내독소가 혈액 전해질 농도의 항상성 혼란을 유도하여 이러한 생리적 불균형은 치명적 상황과 그로인한 죽음을 야기할 수도 있음을 시사하고 있다. We studied the effects of Escherichia coli(E, coli) endotoxin on blood electrolytes levels in rabbits. Endotoxin(E, coli serotype O55: B5) was injected via rabbits' ear vein: 0.10mg/kg(Group A) or 0.50 mg/kg(Group B). Blood samples were taken at postendotoxemic 3, 6,12 and 24 hrs and were analyzed for detections of the levels of blood electrolytes such as Ca++, Mg++,Na+, K+ and CI-. As compared to control group, in endotoxin-treated rabbits Ca++, levels elevated at 6 hrs but decreased at 24 hrs, Mg++ levels were high at 3,6 and 12 hrs, Na+ and K+levels increased at all sampling times and CI- levels rose at 3, 12 and 24 hrs (p<0.05). Interestingly, endotoxic rabbits having hypermagnesemia (about 4.0 mg/dL) showed severe syndromes such as increased secretion, shock, tachypnea, seizure and/or diarrhea, suggesting that these may be clinical signs of imminent death in rabbits. These observations testify that bacterial endotoxin leads to dyshomeostasis of blood electrolytes and the physiological imbalances may cause fatal disorders and subsequent death.