대전·충남지역 고등학교 1학년생의 풍진 항체보유율과 백신접종 후의 항체형성률

오성균,김수영,여경오,김윤태,최명한,이석구,조영채,이동배,이태용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

For the purpose of investigating the positive rate of rubella antibody and sero-conversion rate after rubella vaccination, 863 high school students of the two regions were studied from April 21st, 1996 to September 20th, 1996. The main results were as follows; 1. Positive rate of IgG in male was higher than in female, and the rate of IgM was also higher in male than in female. 2. Positive rate of IgG by blood type was similar, but positive rate of IgM was higher in AB type than the others. 3. Positive rate of IgG by vaccination was similar to all groups, but positive rate of IgM was higher in which was not vaccinated group. 4. Positive rate of IgG and IgM, having vaccination card, was less than which was not having vaccination card. 5. Those who were vaccinated acquired IgG(+) regardless of an existence of IgG antibody, and 28.3% of IgG(-), IgM(+) male who was not vaccinated became IgM(+), IgG(+) after 3months later. As a result positive rate of male was higher than female. The author conclude that vaccination is necessary to prevent from rubella for them.

Chlorella vulgaris의 세포막에서 일어나는 Glycine 흡수과정에 관한 연구

權五溶,辛昌男,吳炅煥,李錦洙 충남대학교 자연과학연구소 1981 忠南科學硏究誌 Vol.8 No.1

In order to datemine that glycine uptake in the cell membrane of Chlorella vulgaris is depend on the specificity of substrate and energy required, it has been investigated on the reaction velocity of glycine and certain carrier. Some effects on the transmural fluxes of glycine across the cell membrane were also detemined. 1. The time course of glycine uptake was a linear function of time to early 10 min, and increased in the steady state slowly. 2. Glycine influx had shown apparent affinity in the range of 0.001 mM to 10 mM solution of glycine and followed Michaelis-Menten Kinetics(Km-0.16 mM, Vmax=0.31 nM2.0x10^7 cells min). 3. Glycine influx with 1 mA IAA was reduced about 50 percentage, suggesting that glycine uptake process is energy-dependent. 4. Glycine uptake was inhibited by glutamic acid, alanine, phenylalanine, proline, and methionine with the exception of histidine. It was suggested that the inhibition was competeted with some amino acids described above.

근관 충전재 및 치근단 역충전재의 방사선 불투과성에 관한 비교 연구

김용상,김서경,황윤찬,황인남,오원만 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.2

This study was performed to assess the radiopacity of a variety of canal filling and retrograde root-end filling materials according to the specification concerning root canal obturation materials. Ten materials including Gutta-percha pellets, amalgam, Fuji II LC, Dyract^(ⓡ) AP, Super EBA^(ⓡ), IRM^(ⓡ) AH 26^(ⓡ), Sealapex™, Tubli-Seal™ and dentin were evaluated in this study In the first part, densitometric reading of an each step of aluminum step wedge on occlusal film were performed at 60 kVp (0.2, 0.3, 0.4 s), 70 kVp (0.2, 0.3, 0.33 s) to decide appropriate voltage and exposure time. In the second part, ten specimens which are 5 ㎜ in diameter and 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 ㎜ in thickness, were fabricated from each material studied. The specimens were radiographed simultaneously with an aluminum step wedge under decided condition (60 kVp, 0.2 s). The mean radiographic density values of the materials were transformed into radiopacity expressed equivalent thickness of aluminum (㎜ Al). The following results were obtained. 1. Among the various conditions including 0.2 s, 0.3 s, 0.4 s at 60 kVp and 0.2 s, 0.3 s, 0.33 s at 70 kVp, the appropriate voltage and exposure time that meet the requirement of density from 0.5 to 2.0 was 0.2 s at 60 kVp. 2. All of the materials in this study had greater radiopacity than the minimun level recommended by ISO No. 4049 standards. 3. Most of the materials had greater radiopacity than 3 ㎜ Al requirement of ANSI/ADA specification No. 57 (2000) and ISO No. 6876 (2001) standards except for Fuji II LC and Dyract. It suggests that all experimental canal filling and retrograde root-end filling materials have a Sufficient radiopacity that meet the requirement concerning root canal obturation materials except for Fuji II LC and Dyract. 본 실험에서는 다양한 방사선 노출 조건에 따른 aluminum step wedge에 대한 광학 밀도를 알아보고, 그 중 적절한 노출조건을 선택하여 수종의 근관 충전재와 치근단 역충전재의 방사선 불투과성 정도를 굉가, 비교하였다. 방사선 불투과성의 기준을 위해 11개의 step으로 구성된 aluminum step wedge를 제작하여 , 60 kVp, 70 kVp 관전압 상태에서 각각 0.2. 0.3, 0.4초와 0.2, 0.3, 0.33초의 노출시간으로 교합 필름상에서 방사선 촬영 후 적절한 노출 조건을 구하였다. 직경 5 ㎜,각각의 두께 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 ㎜인 10종 (Gutta-percha, amalgam, Super EBA^(ⓡ), MTA, IRM^(ⓡ), Fuji II LC, Dyract^(ⓡ) AP, AH 26^(ⓡ), Sealapex^(ⓡ), Tubli-Sea™의 근관 충전재와 치근단 역충전재 시편을 각 재료와 두께당 10개씩 제작한 후, 동일한 두께의 상아질 시편 , aluminum step wedge와 함께 정해진 노출조건에 따라 방사선 촬영을 하였다. 모든 필름은 자동현상기로 현상하였다. 시편의 흑화도를 densitometer (Model 07-443, Victoreen Inc, Cleveland, Ohio, USA)로 5회 반복 측정 후, 평균값을 구하여 회귀분석 후 알루미늄 두께로 환산하였다. 얻어진 정보를 분석하여 다음의 결과를 얻었다. 1. 관전압 60 kVp에서 노출시간 0.2, 0.3, 0.4초, 70 kVp에서 0.2, 0.3, 0.33초로 변화를 주어 방사선 촬영을 하였을 때, 흑화도가 0.5 - 2.5 사이여야 한다는 ISO No. 6876 규격에 가장 적합한 것은 60 kVp, 0.2초일 경우였다. 2.측정된 근관 충전재와 치근단 역충전재들의 방사선 불투과성 모두 ISO No. 4049 규격에 적합하였다. 3.광중합형 글래스 아이오노머 (Fuji II LC)와 컴포머 (Dyract)를 제외하고는 ANSI/ADA specification (2000) 또는 ISO No. 6876 (2001)규격이 제시한 최소한 3 ㎜ Al 이상의 방사선 불투과성을 지녀야 한다는 규격에 적합하였다. 이상의 결과는 본 실험에 사용된 수종의 근관 충전재와 치근단 역충전재 중 Fuji Ⅱ LC와 Dyract를 제외하고 모두 규격에 적합한 방사선 불투과성을 가지고 있음을 시사한다.

여고생의 건강증진생활양식 수행정도에 관한 연구

홍춘실,오경옥,이선영,차규정,김선애 충남대학교 간호과학연구소 2002 충남대 간호학술지 Vol.5 No.1

The purpose of the study was to examine the performance level of and the cognitive factors of health-promoting lifestyle in high school girls. This study was designed to provide basic data for development of nursing plan and effective intervention program for health promotion. The results were; (1) The mean score of health-promoting lifestyle profile was 2.61 (possible range 1-4) (2) Performance level of each subscale was scored as follows, interpersonal support(3.01), nutrition(2.74), self-actua!ization(2.68), stress management(2.55), health responsibility(2.35), and exercise(2.11). (3) The mean scores of the study variables (possible range 1-5) were relatively high in this sample, the mean of self-efficacy was 3.24; health locus of control was 2.97; perceived health status was 3.03. (4) The health-promoting lifestyle profile had a significant positive correlation with self-efficacy (r=.521, p<.001), health locus of control (r=.236, p<001), and perceived health status(r=.156, p<.01). The study showed the needs to intervene to improve the performance of health-promoting lifestyle of high school girls. Further study is recommended to develop and apply health improvement program especially for less performed dimensions such as exercise and health responsibility. Also, it is recommended to examine other cognitive-perceptual factors influencing health-promoting lifestyle.

중국에서 이식한 종어 (Leiocassis longirostris Gunther)의 외부 형태 및 초기생활사

이완옥,김경환,김대희,조용철,김응오 한국어류학회 2004 韓國魚類學會誌 Vol.16 No.1

한국에서 절멸된 것으로 알려진 종어 (Leiocassis longifostris)를 국내에 복원하고, 양식대상종으로 개발하기 위하여 중국에서 도입하여 외부형태를 비교하고, 난발생 과정 및 부화자어의 형태를 연구하였다. 도입된 표본의 계수·계측형질을 국내와 중국에 알려진 문헌과 비교한 결과 큰 차이를 보이지 않았다. 중국에서 도입한 암수 한쌍의 성어에서 채란하였고, 습식법으로 수정하였다. 수정란의 크기는 2.01∼2.10 mm(n=10)이었으며, 알의 형태는 원반형이고, 난막은 무색투명하였으며, 강한 접착력이 있었다. 난황은 담황색이고 유구는 없었다. 수정 1시간 후에 배반이 완성되었고, 1시간 30분 후에 난할이 시작되었다. 수정란의 수온을 21.5∼23.0℃로 유지할 때 30∼50분 간격으로 이후 난할이 계속되었다. 수정 후 상실기는 6시간에 도달하였고, 낭배기는 20시간 20분 후에 완성되었다. 난황 표면에서 관찰되는 특이한 연동운동은 상실기에 시작되어 포배기 말기인 30% epiboly기까지 계속되었다. 수정 75시간 후에 부화에 이르렀고, 부화 직후 자어의 크기는 전장 5.91∼6.10 mm 이었다. 부화 3일 후에 입이 열리고, 수염과 부레가 완성되었으며, 전장 9.07 ∼9.37 mm까지 자랐다. 부화 15일후에는 모든 지느러미가 완성되어 후기자어기 상태이었고, 전장 14.80∼16.50 mm로 자랐으며, 부화 25일 후에는 체형, 체색 그리고 생활습성 등이 성체와 유사하고, 전장은 18.59∼22.35 mm까지 성장하였다. The Long-snout bullhead, Leiocassis longirostris, is an introduced fish from China which had become extinct in Korea. Its morphological characteristics and early life history was studied to obtain useful information required for restoration of the species and also for its utilization as an aquaculture fish. Measurements and counts of specimens introduced into Korea were included in the range of those taken from the known literature of Korea and China. Eggs were obtained by fertilization by wet method in the laboratory using one mature male and female adults of introduced L. longirostris. The fertilized eggs measured 2.01-2.10 ㎜ (n = 10) in diameter. The eggs were diskshaped and strongly adhesive, with colorless transparent membrane. The yolk was pale yellow in color with no oil globule. The blastodisc was formed in about 1 hour and cleavage started around 1 hour 30 minutes after fertilization. The cleavage intervals were about 30-50 minutes at 21.5-23℃. The morula stage was reached at 6 hours, and gastrulation was completed at 20 hours 20 min. after fertilization. Peristalsic movements of the yolk surface started at the morula stage and continued to the blastula stage (30% epiboly). Hatching occurred 75 hours after fertilization, and newly hatched larvae were 5.91-6.10 ㎜ (n = 10) in total length (TL). The mouth was open, and barbels and the air-bladder were completely formed 3 days after hatching, at 9.07-9.37 ㎜ TL. All the fin rays were completely formed and the larvae became a postlarva 15 days after hatching at 14.80-16.50 ㎜ in TL. Body shape, color pattern, and habitat behavior were similar to those in the adult at 25 days after hatching and a size of 18.59-22.35 ㎜ TL.

IMAGES OF THE ISSUE : A Patient with Hematochezia and Intestinal Obstruction

( Kyong Yong Oh ),( Yoon Jae Kim ) 대한장연구학회 2014 Intestinal Research Vol.12 No.3

Aortic Regurgitation from Right Coronary Cusp Prolapse Associated with Ventricular Septal Defect in Adult (초)

( Kyong Yong Oh ),( Wook Jin Chung ),( Mi Seung Shin ),( Woong Chol Kang ),( Seung Hwan Han ),( Chan Il Moon ),( Tae Hoon Ahn ),( In Suck Choi ),( Eak Kyun Shin ) 대한내과학회 2010 대한내과학회 추계학술대회 Vol.2010 No.-

Inhibition of Sphingolipid Metabolism Enhances Resveratrol Chemotherapy in Human Gastric Cancer Cells

( Kyong Oh Shin ),( Nam Young Park ),( Cho Hee Seo ),( Seon Pyo Hong ),( Ki Wan Oh ),( Jin Tae Hong ),( Sang Kil Han ),( Yong Moon Lee ) 한국응용약물학회 2012 Biomolecules & Therapeutics(구 응용약물학회지) Vol.20 No.5

Resveratrol, a chemopreventive agent, is rapidly metabolized in the intestine and liver via glucuronidation. Thus, the pharmacokinetics of resveratrol limits its efficacy. To improve efficacy, the activity of resveratrol was investigated in the context of sphingolipid metabolism in human gastric cancer cells. Diverse sphingolipid metabolites, including dihydroceramides (DHCer), were tested for their ability to induce resveratrol cytotoxicity. Exposure to resveratrol (100 mM) for 24 hr induced cell death and cell cycle arrest in gastric cancer cells. Exposure to the combination of resveratrol and dimethylsphingosine (DMS) increased cytotoxicity, demonstrating that sphingolipid metabolites intensify resveratrol activity. Specifically, DHCer accumulated in a resveratrol concentration- dependent manner in SNU-1 and HT-29 cells, but not in SNU-668 cells. LC-MS/MS analysis showed that specific DHCer species containing C24:0, C16:0, C24:1, and C22:0 fatty acids chain were increased by up to 30-fold by resveratrol, indicating that resveratrol may partially inhibit DHCer desaturase. Indeed, resveratrol mildly inhibited DHCer desaturase activity compared to the specific inhibitor GT-11 or to retinamide (4-HPR); however, in SNU-1 cells resveratrol alone exhibited a typical cell cycle arrest pattern, which GT-11 did not alter, indicating that inhibition of DHCer desaturase is not essential to the cytotoxicity induced by the combination of resveratrol and sphingolipid metabolites. Resveratrol-induced p53 expression strongly correlated with the enhancement of cytotoxicity observed upon combination of resveratrol with DMS or 4-HPR. Taken together, these results show that DHCer accumulation is a novel lipid biomarker of resveratrol-induced cytotoxicity in human gastric cancer cells.

A Case of Primary Hyperparathyroidism Caused by Solitary Parathyroid Adenoma Localized with Computerized Tomography (초)

( Kyong Yong Oh ),( Hyon Seung Yi ),( Young Sil Eom ),( Si Hoon Lee ),( Ie Byung Park ) 대한내과학회 2010 대한내과학회 추계학술대회 Vol.2010 No.-

Ginsenoside compound K inhibits angiogenesis via regulation of sphingosine kinase-1 in human umbilical vein endothelial cells

Shin, Kyong-Oh,Seo, Cho-Hee,Cho, Hyo-Hyun,Oh, Seikwan,Hong, Seon-Pyo,Yoo, Hwan-Soo,Hong, Jin-Tae,Oh, Ki-Wan,Lee, Yong-Moon 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.9

Ginsenoside compound K (CK) is a metabolite of the protopanaxadiol-type saponins of Panax ginseng C.A. Meyer (Araliaceae), has long been used to treat against the development of cancer, inflammation, allergies, and diabetes. This study examined the anti-angiogenic properties of CK against sphingosine 1-phosphate (S1P)-induced cell migration via regulation of sphingosine kinase 1 (SPHK1) in human umbilical vein endothelial cells (HUVEC). Studies on S1P-induced cell migration, expression of SPHK1 and MMPs and analysis of sphingolipidmetabolites by LC-MS/MS were examined after the treatment of CK (2.5, 5, $10{\mu}g/mL$) in HUVEC. S1P produced by SPHK1 is also involved in cell growth, migration, and protection of apoptosis; therefore, we sought to investigate whether ginsenosides are able to regulate SPHK1. For this purpose, we developed an inhibitory assay of SPHK1 activity and an analytical method for detection of S1P and other sphingolipid metabolites in HUVEC. Ginsenoside CK inhibited 100 nM S1P-induced cell migrations in a dose-dependent manner. Among tested ginsenosides, CK exclusively inhibited S1P production, SPHK1 activity and SPHK1 expression in HUVEC, whereas expression of the pro-apoptotic sphingolipids, sphingosine and ceramide, was increased in response to CK. The major subspecies of the increased ceramide was C24:0-ceramide. CKalso disrupted the sphingolipid rheostat, which ultimately influences cell fate, and dose-dependently inhibited HUVEC migration by reducing expression of metalloproteinases (MMPs). Ginsenoside CK acts as a unique HUVEC migration inhibitor by regulating MMP expression, as well as the activity of SPHK1 and its related sphingolipid metabolites.