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Simultaneous determination of methoxyflavones in selected Korean thistles
( Joyce P. Rodriguez ),( Norman G. Quilantang ),( Ju Sung Lee ),( Jun Yeon Park ),( Jae Suk Shim ),( Ki Sung Kang ),( Eun Ju Cho ),( Paul John L. Geraldino ),( Sanghyun Lee ) 한국응용생명화학회(구 한국농화학회) 2018 Journal of Applied Biological Chemistry (J. Appl. Vol.61 No.3
Simultaneous determination of three methoxyflavones, namely, cirsimarin (1), hispidulin (2), and cirsimaritin (3) in selected Korean thistles was performed via reversed-phase high performance liquid chromatography system. Compound 1 was present in all the thistle species examined, whereas 2 and 3 were only detected in Cirsium japonicum and C. japonicum var. maackii (CJM). The concentration of compounds 1-3 in CJM varied according to the time of harvest. Plants collected in the spring (CJMS) and fall (CJMF) had the highest contents of 3 and 1, respectively. A lower content of 2 was observed in CJMF than in CJMS. This indicates that seasonal variation affects the flavonoid content of CJM. The results of this study show that CJM is an excellent source of compounds 1-3 and it can potentially be cultivated for industrial and pharmaceutical applications involving these compounds.
( Jaemin Lee ),( Hyun-sung Ryu ),( Joyce P. Rodriguez ),( Sanghyun Lee ) 한국응용생명화학회 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.1
The methanol extract of Rhododendron mucronulatum for. albiflorum (RMFA) stems inhibited aldose reductase (AR) activity. The RMFA fractions obtained by stepwise extraction with solvents of different polarity were tested for AR inhibition in vitro using the lens of a rat. Among them, the ethyl acetate (EtOAc) fraction inhibited AR more than the other fractions. Quercetin (1) from the EtOAc fraction showed a high AR inhibition with IC<sub>50</sub> of 2.11 μM. The stems of RMFA contained the highest amount (5.12 mg/g extract) of quercetin. Our results suggest that RMFA, which contained quercetin, could be a useful material for the development of supplementary functional foods.
Antifungal activity of pinosylvin from Pinus densiflora on turfgrass fungal diseases
( Dong Gu Lee ),( Seong Jun Lee ),( Joyce P. Rodriguez ),( Ik Hwi Kim ),( Taehyun Chang ),( Sanghyun Lee ) 한국응용생명화학회(구 한국농화학회) 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.3
The objective was to examine the antifungal activity of Pinus densiflora extract for the control of turfgrass fungal diseases. Antifungal activities of the various fractions of n-hexane, methylene chloride (Ch), ethyl acetate (EtOAc), and n-butanol from P. densiflora were evaluated against Rhizoctonia solani AG1-1B, R. solani AG2-2IV, Sclerotinia homoeocarpa, R. cerealis, Pythium spp., and Colletotrichum graminicola. The Ch and EtOAc fractions showed antifungal activity against Pythium sp. and C. graminicola in paper disc assay. The effective concentration to produce 50% mycelial inhibition (EC<sub>50</sub>) using five discriminatory concentrations of pinosylvin (1) from the Ch fraction of P. densiflora was evaluated on R. solani AG1-1B, R. solani AG2- 2IV, R. cerealis, and S. homoeocarpa. S. homoeocarpa showed the highest sensitivity with the lowest mean EC50 value (8.426 μg/ mL) among the four pathogens. Among the three Rhizoctonia pathogens, R. cerealis had the highest mean EC50 value (99.832 μg/mL) and R. solani AG2-2IV, with the lowest sensitivity, had the lowest EC<sub>50</sub> value (39.696 μg/mL). These results suggested that pinosylvin (1) from P. densiflora could be a valuable lead compound in the improvement of a novel antifungal agent.
Yeon Soo Lee,Joyce H. Keyak,Thay Q. Lee 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5
This study aimed to assess the stress change inside the patella after implantation of an polyethylene patella button. Finite elements models of the patellae before and after implantation of patellar button were created. Experimentally determined spring constants of muscles and ligaments, and patellofemoral contacting loads were applied to the models at 30°, 60°, and 90° of knee flexion. The Von Mises stress of the intact patella decreased with increased knee flexion, while that of implanted patella increased. Also, the stress scale in the implanted patella was 3~9 times higher than in the intact one. The highly stressed region of the intact patella moved proximally with higher knee flexion angles, while that of the implanted model stayed near the central anterior patella. At 90° of knee flexion, the stress in the anterodistal patella increased considerably after implantation of a patella button so that the anterodistal patella may be susceptible to be painful source.
Hong, Riwon,Sur, BongJun,Yeom, Mijung,Lee, Bombi,Kim, Kyoung Soo,Rodriguez, Joyce P.,Lee, Sanghyun,Kang, Ki Sung,Huh, Chang-Ki,Lee, Sang Cheon,Hahm, Dae-Hyun Elsevier 2018 Phytomedicine Vol.38 No.-
<P>Conclusion: These results suggest that the ethanolic extract of ACI have significant anti-inflammatory and anti-arthritic effects in a rodent arthritis model and in IL-1 beta-stimulatedFLS cells. Thus, ACI may be a useful candidate for developing pharmaceuticals or dietary supplements for the treatment of inflammatory arthritis.</P>
Lee, Yunki,Le Thi, Phuong,Seon, Gyeung Mi,Ryu, Seung Bae,Brophy, Colleen M.,Kim, YongTae,Park, Jong-Chul,Park, Ki Dong,Cheung-Flynn, Joyce,Sung, Hak-Joon Elsevier 2017 Journal of controlled release Vol.266 No.-
<P><B>Abstract</B></P> <P>The leading cause of synthetic graft failure includes thrombotic occlusion and intimal hyperplasia at the site of vascular anastomosis. Herein, we report a co-immobilization strategy of heparin and potent anti-neointimal drug (Mitogen Activated Protein Kinase II inhibitory peptide; MK2i) by using a tyrosinase-catalyzed oxidative reaction for preventing thrombotic occlusion and neointimal formation of synthetic vascular grafts. The binding of heparin–tyramine polymer (HT) onto the polycarprolactone (PCL) surface enhanced blood compatibility with significantly reduced protein absorption (64.7% decrease) and platelet adhesion (85.6% decrease) compared to bare PCL surface. When loading MK2i, 1) the HT depot surface gained high MK2i-loading efficiency through charge-charge interaction, and 2) this depot platform enabled long-term, controlled release over 4weeks (92–272μg/mL of MK2i). The released MK2i showed significant inhibitory effects on VSMC migration through down-regulated phosphorylation of target proteins (HSP27 and CREB) associated with intimal hyperplasia. In addition, it was found that the released MK2i infiltrated into the tissue with a cumulative manner in <I>ex vivo</I> human saphenous vein (HSV) model. This present study demonstrates that enzymatically HT-coated surface modification is an effective strategy to induce long-term MK2i release as well as hemocompatibility, thereby improving anti-neointimal activity of synthetic vascular grafts.</P> <P>Graphical abstract</P> <P>[DISPLAY OMISSION]</P>
PKB phosphorylates p27, impairs its nuclear import and opposes p27 - mediated G1 arrest
JINHWA LEE,JIYONG LIANG,JOYCE M. SLINGERLAND 한국생명과학회 2002 한국생명과학회 심포지움 Vol.37 No.-
PKB activation may contribute to resistance to antiproliferative signals and breast cancer progression in part by impairing nuclear import and action of p27. PKB transfection caused cytoplasmic p27 accumulation and cytokine resistance. The nuclear localization region of p27 contains a PKB/Akt consensus site at threonine 157 and p27 phosphorylation by PKB impaired its nuclear import in vitro. PKB/Akt phosphorylated wild type p27 but not p27T157A. PKB activation led to cytoplasmic mislocalization of p27WT but p27T157A remained nuclear. In PKB activated cells, p27WT failed to cause G1 arrest, while the anti proliferative effect of p27T157 A was not impaired. Cytoplasmic p27 was seen in 41% (52/128) of primary human breast cancers in association with PKB activation. Thus, we show a novel mechanism whereby PKB impairs p27 function that is associated with an aggressive phenotype in human breast cancer.