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Antifungal activity of pinosylvin from Pinus densiflora on turfgrass fungal diseases
( Dong Gu Lee ),( Seong Jun Lee ),( Joyce P. Rodriguez ),( Ik Hwi Kim ),( Taehyun Chang ),( Sanghyun Lee ) 한국응용생명화학회(구 한국농화학회) 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.3
The objective was to examine the antifungal activity of Pinus densiflora extract for the control of turfgrass fungal diseases. Antifungal activities of the various fractions of n-hexane, methylene chloride (Ch), ethyl acetate (EtOAc), and n-butanol from P. densiflora were evaluated against Rhizoctonia solani AG1-1B, R. solani AG2-2IV, Sclerotinia homoeocarpa, R. cerealis, Pythium spp., and Colletotrichum graminicola. The Ch and EtOAc fractions showed antifungal activity against Pythium sp. and C. graminicola in paper disc assay. The effective concentration to produce 50% mycelial inhibition (EC<sub>50</sub>) using five discriminatory concentrations of pinosylvin (1) from the Ch fraction of P. densiflora was evaluated on R. solani AG1-1B, R. solani AG2- 2IV, R. cerealis, and S. homoeocarpa. S. homoeocarpa showed the highest sensitivity with the lowest mean EC50 value (8.426 μg/ mL) among the four pathogens. Among the three Rhizoctonia pathogens, R. cerealis had the highest mean EC50 value (99.832 μg/mL) and R. solani AG2-2IV, with the lowest sensitivity, had the lowest EC<sub>50</sub> value (39.696 μg/mL). These results suggested that pinosylvin (1) from P. densiflora could be a valuable lead compound in the improvement of a novel antifungal agent.
Norman G. Quilantang,류서현,박세훈,변재상,전제성,이주성,Joyce P. Rodriguez,윤영석,Sanghyun Lee,이상현 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.6
The methanol extracts of flowers obtained from 67 plant species were screened for their inhibitory activity on aldose reductase(AR). Alnus japonica , Aster spathulifolius , Chionanthus retusus , Morus bombycis , Crysanthemum boreale , Persicariatinctoria , Platycarya strobilacea , and Serratula coronata var. insularis exhibited potent aldose reductase inhibitory (ARI)activity. HPLC-UV analysis of quercetin, an AR inhibitory flavonoid, was performed on extracts showing strong ARI activity. Quercetin was detected in C. retusus , C. boreale , P. tinctoria , and S. coronata var. insularis at concentrations of 1.33,1.56, 0.82, and 3.37 mg g −1 extract, respectively, indicating that quercetin contributed to the ARI activity of these extracts. In the samples in which quercetin was absent, other compounds may be responsible for their potent ARI activity. Theseresults serve as a basis for further studies regarding the bioactive components responsible for the inhibitory eff ects of variousfl ower extracts on AR activity.
Simultaneous determination of methoxyflavones in selected Korean thistles
( Joyce P. Rodriguez ),( Norman G. Quilantang ),( Ju Sung Lee ),( Jun Yeon Park ),( Jae Suk Shim ),( Ki Sung Kang ),( Eun Ju Cho ),( Paul John L. Geraldino ),( Sanghyun Lee ) 한국응용생명화학회(구 한국농화학회) 2018 Journal of Applied Biological Chemistry (J. Appl. Vol.61 No.3
Simultaneous determination of three methoxyflavones, namely, cirsimarin (1), hispidulin (2), and cirsimaritin (3) in selected Korean thistles was performed via reversed-phase high performance liquid chromatography system. Compound 1 was present in all the thistle species examined, whereas 2 and 3 were only detected in Cirsium japonicum and C. japonicum var. maackii (CJM). The concentration of compounds 1-3 in CJM varied according to the time of harvest. Plants collected in the spring (CJMS) and fall (CJMF) had the highest contents of 3 and 1, respectively. A lower content of 2 was observed in CJMF than in CJMS. This indicates that seasonal variation affects the flavonoid content of CJM. The results of this study show that CJM is an excellent source of compounds 1-3 and it can potentially be cultivated for industrial and pharmaceutical applications involving these compounds.
Hong, Riwon,Sur, BongJun,Yeom, Mijung,Lee, Bombi,Kim, Kyoung Soo,Rodriguez, Joyce P.,Lee, Sanghyun,Kang, Ki Sung,Huh, Chang-Ki,Lee, Sang Cheon,Hahm, Dae-Hyun Elsevier 2018 Phytomedicine Vol.38 No.-
<P>Conclusion: These results suggest that the ethanolic extract of ACI have significant anti-inflammatory and anti-arthritic effects in a rodent arthritis model and in IL-1 beta-stimulatedFLS cells. Thus, ACI may be a useful candidate for developing pharmaceuticals or dietary supplements for the treatment of inflammatory arthritis.</P>
Joyce P. Rodriguez,이재민,박준연,강기성,함대현,이상천,이상현 한국응용생명화학회 2017 Applied Biological Chemistry (Appl Biol Chem) Vol.60 No.5
This study was conducted to optimize the extraction conditions of flavonoids from Cirsium japonicum var. maackii (ICF-1). The effects of sample material ratio, solvent concentration, extraction time, solid-to-solvent ratio, and number of extractions on flavonoid extraction efficiency were analyzed. Three flavonoids were specifically investigated: cirsimarin (1), hispidulin (2), and cirsimaritin (3). In single-factor experiments, each variable had a significant effect on the determination of content of compounds 1–3. The optimal conditions for extraction were found to be: mass, 15 g; ratio of spring and fall leaves, 4:1; extraction solvent, 70% ethanol; extraction time, 4 h; solid-to-solvent ratio, 1:20; and number of extractions, 1. The results of the study were used to maximize the potential of ICF-1 samples and optimize the efficiency of the extraction process.
Determination of Silybin B in the Different Parts of Silybum marianum using HPLC-UV
Joyce P. Rodriguez,Norman G. Quilantang,이주성,이정민,김현영,심재숙,이상현 한국생약학회 2018 Natural Product Sciences Vol.24 No.2
Silymarin is the standardized extract from Silybum marianum which consists mainly of flavonoids and polyphenols. It is highly regarded for its hepatoprotective ability. Silybin B is a flavonolignan and one of the active components of silymarin. The content of silybin B in various parts of S. marianum was analyzed by HPLC-UV. Results show that the extract of seeds contain the highest amount of silybin B (7.434 mg/g DW). The petioles of S. marianum showed a low content of silybin B. This study revealed that seeds of S. marianum contain high amount of silybin B and could be a good source of the compound.
Fatty Acid Content in Perilla Cultivars and Commercial Oils Determined by GC Analysis
이재민,Joyce P. Rodriguez,김유정,이명희,조은주,이상현 한국생약학회 2016 Natural Product Sciences Vol.22 No.4
The content analysis of fatty acids in Perilla cultivars and commercial oils is conducted through gas chromatography with a flame ionization detector. Results show that Perilla cultivars, such as Deulsaem and Daesil, contain high amounts of a-linolenic acid (262.22 and 261.97 mg/g, respectively). Among commercial oils, Perilla oil contains a higher amount of a-linolenic acid (515.20 mg/g). Accordingly, a-linolenic acid is a major fatty acid of Perilla cultivars and oil. Therefore, Perilla cultivars could be used as a food supplement for nutritional and pharmaceutical purposes.
이재민,Joyce P. Rodriguez,Norman G. Quilantang,이명희,조은주,Sonia D. Jacinto,이상현 한국응용생명화학회 2017 Applied Biological Chemistry (Appl Biol Chem) Vol.60 No.2
Perilla frutescens var. japonica (PF) is an annual aromatic herb has been consumed as a food ingredient and medicinal crop in Asian countries. To evaluate the therapeutic efficacy of aldose reductase (AR) inhibition, we tested the PF seeds. The stepwise polarities of PF were tested for AR inhibition, and we determined the CH2Cl2 and EtOAc fractions to be good inhibitors (5.81 and 3.99 lg/mL, respectively). Compounds 1–3 were isolated from the CH2Cl2 and EtOAc fractions and identified as luteolin (1), apigenin (2), and diosmetin (3) by physicochemical and spectroscopic data. Among them, luteolin (1) and apigenin (2) had high AR inhibitory activity (1.89 and 4.18 lM). Deulsaem, a variety of PF, was determined to have the highest flavonoid content among ten PF seeds tested (2.10 mg/g). This study suggests that PF could be utilized as a natural source to treat diabetic complications.
이재민,Joyce P. Rodriguez,이강희,박준연,강기성,함대현,허창기,이상천,이상현 한국응용생명화학회 2017 Applied Biological Chemistry (Appl Biol Chem) Vol.60 No.5
The therapeutic activities of flavonoids from natural plant sources were investigated. The ethanol extracts from the aerial parts of Cirsium japonicum var. maackii (CJM) were tested for aldose reductase inhibition (ARI). Additionally, stepwise polarity fractions and flavonoids from CJM were evaluated for ARI. The ethyl acetate (EtOAc) fraction from CJM showed significant inhibitory effects. The compounds in the EtOAc fraction were identified as the flavonoids-cirsimaritin (1), hispidulin (2), and cirsimarin (3). Based on an ARI assay, the EtOAc fraction and hispidulin (2) exhibited good AR inhibitory activity (IC50 values of 0.21 lg/mL and 0.77 lM, respectively). An HPLC quantitative analysis of different parts of CJM showed that the aerial part collected in the spring season (CJL1) contains the highest total flavonoid content. These results serve as a basis for maximizing the flavonoid yield and for the efficient usage of various parts of CJM. Our results also suggest that CJM could be a useful ARI material for the treatment of various diabetic complications.
Determination of flavonoids in Acer okamotoanum and their aldose reductase inhibitory activities
이재민,이동구,Joyce P. Rodriguez,박준언,조은주,Sonia D. Jacinto,이상현 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.1
Plants in the genus Acer include medicinal plants and deciduous tree species in which several bioactive compounds have been isolated. In this study, flavonoids were isolated from the aerial parts of Acer okamotoanum by open column chromatography and identified by spectroscopic analysis as afzelin, quercitrin, and isoquercitrin. Among these flavonoids, quercitrin and isoquercitrin exhibited potent aldose reductase inhibitory activity (IC50 value of 0.40 and 0.63 μM, respectively). Almost all studied tissues of Acer spp. contained high amounts of quercitrin. The leaf of A. mono had the highest amount of quercitrin (61.90 μg g−1). Our results demonstrated that Acer species contain high concentrations of flavonoids with promising aldose reductase inhibitory activity that could be utilized to develop novel therapeutics for diabetic disorders.