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        사염화탄소로 중독된 생쥐의 간독성에 대한 키토산올리고당의 효과

        황구연,윤중식,김영호,정민주,노영복,Hwang, Koo-Yeon,Yoon, Jung-Sik,Kim, Young-Ho,Chung, Min-Ju,Roh, Young-Bok 한국현미경학회 1999 Applied microscopy Vol.29 No.3

        사염화탄소가 간 미세구조에 미치는 영향에 대한 키토산올리고당의 억제효과를 연구하고자 실험군을 3개군(group A, B, C)으로 구분하였다. A군에는 키토산올리고당(0.3% solution)을 7일간 전처치하고, 사염화탄소(0.3 ml/kg)을 복강투여 하였다. B군에는 키토산올리고당과 사염화탄소를 복강투여 하였다. C군에는 사염화탄소만을 투여하였다. 그리고 각 군은 사염화탄소 투여 후 24, 48, 72, 96시간 경과한 간세포의 변화를 전자현미경으로 관찰하여 다음과 같은 결론을 얻었다. A군에서 핵의 핵막은 전 시간대에 걸쳐 비교적 원형을 유지하였고, 사립체도 정상소견을 보였다. 조면소포체는 48시간대까지 층판구조가 약간 파괴되었지만 리보소옴은 막에 부착된채로 관찰되었다. 그리고 72시간부터는 전형적인 층판구조를 이루었다. 평활막성 소포는 관찰되지 많았다. 그리고 용해소체가 72시간대까지 세포질에서 관찰되었다. 96시간대에서는 세포 소기관들이 정상적인 소견을 보여주었다. B군에서 핵막의 변형은 C군보다 비교적 덜하고, 사립체는 전반적으로 정상적인 소견을 보여주었다. 조면소포체의 일부는 층판구조를 형성하고, 다른 일부는 수조가 팽대되었지만 리보소옴이 부착되어 있었다. 그리고 지방적이 24시간대에서 관찰되었다. 글리코겐이 48시간부터 세포질에서 관찰되었고, 72시간부터 용해소체도 관찰되었다. C군에서는 핵의 핵막이 전 시간대에서 매우 불규칙하고 핵질의 응축현상이 관찰되었다. 조면소포체는 층판 구조가 완전히 파괴되었고, 평활막성 소포가 세포질에서 관찰되었다. 사립체들은 비교적 독성에 영향을 덜 받은 것으로 사료된다. 그리고 24시간대부터 크고 작은 지방적이 세포질 전반에 걸쳐 분포하였다. 이상의 결과를 종합하면 키토산올리고당이 mouse간 세포에 미치는 사염화탄소의 독성을 감소시키는 것으로 사료된다. This study aims to demonstrate the effect of chitosan oligosaccharide on the ultrastructural changes in the mouse liver toxicated by carbon tetrachloride $(CCl_4)$. A healthy male ICR mouse that weighted $27{\pm}2gm$ was used for experiment. The experimental group was divided into three groups; the group A; the pretreated group with chitosan oligosaccharide, the group B; the simultaneous group, the group C; treated only the $CCl_4$. The group A was simultaneously treated with chitosan oligosaccharide and $CCl_4$ after pretreated with chitosan oligosaccharide for 7 days. The group B injected $CCl_4$ and chitosan oligosaccharide to the intraperitoneal. The group C injected with only $CCl_4$ to the intraperitoneal. The results were as follow: In the group A, the nuclear membrane and the mitochondria were observed almost normal in shapes at overall the time. Some lamellae of the RER (rough endoplasmic reticulum) destructed until 48 hours but ribosome attached. The destructed lamellae reformed at 72 hours but the smooth membrane vesicles not observed. The lysosomes observed at 72 hours. At 96 hours, all organelles showed in normal shapes. In the group B, changes of nuclear membranes were relatively lighter than group C. Mitochondria observed normal shape through the time. Parts of RER reformed the lamellae, other parts dilated inner cavity. And lipid droplet observed around the 24 hours. Glycogen and lysosome observed 48 hours and 72 hours, respectively. In the group C, nuclear membrane was irregular and nuclear cytoplasm condensed through the time. The lamellae of RER destructed from 24 to 96 hours. Smooth membrane vesicles observed in the cytoplasm at 48 ours. Mitochondria was less effected by toxic. And from the 24 hours, the variable sizes of lipid droplets observed in tile cytoplasm. These results suggest that chitosan oligosaccharide attenuates the toxic effect of the carbon tetrachloride in the mouse liver.

      • 국민학교 교사의 통합교육과정 운영에 관한 이론적 탐색 : 영향변인 및 의사결정방식

        황구연,김인숙 진주교육대학교 1995 論文集 Vol.39 No.1

        The purpose of this study is to examine and discuss the effect factors on the teacher's management of the elementary school curriculum, to review the preceding researches on the changes of the teacher's role in the curriculum management and to discuss the elementary school teacher's decision-making style on the management of the integrated curriculum. Various effect factors influence the process of teacher's decision making related to the school curriculum management. These factors can be classified by some kinds of standards and the degree of the influence of each factor differs according to the phase of the curriculum management and the characteristics of the school, student and teacher organization. Teacher's roles in the curriculum management are classified into two types : one is the role to believer the fired curriculum and the other is the role to participate in the decision-making process on the curriculum The fact that the 6th revised elementary school curriculum involves more flexibility on the curriculum management suggests that teachers will have more chance to take part in the process of decision making on the curriculum management, This change of teacher role requires that teachers hale more professionalism and autonomy on the curriculum management. All the staff members in school should decide collegially and cooperatively about the curriculum management. Preceding research results show that teachers generally want to participate in the decision-making process in the school, but the needs level of participation depends on the decision domains. On the curriculum management, teachers make a decision at a perspective that is placed between perceived and reflective perspective. The perceived decision-making perspective focuses the division of subject-matters, strict disciplines arid student achievement. In contrast, the reflective decision-making perspective puts emphasis on student's development and individuality, integrated curriculum and flexible curriculum management. Therefore the management style of the integrated curriculum approaches the reflective decision-making perspective.

      • 연령·표상양식·단어유형이 유아산문학습에 미치는 효과

        황구연,최민수,정연숙 진주교육대학교 1990 論文集 Vol.34 No.1

        The learning activities of young children have been frequently accomplished through the prose materials varied in the form and the structure. The probe passages mean the language activities that explain, describe, and tell about the objects and events in several sentences. It is needed the concrete materials and the method of instruction in order that young children understand the abstract and complex informations and concepts in prose. The materials of instruction for young children's prose learning involve the pictures, the actions, and the written language that are similar or identical with the prose contents. Thus it is possible to study the relations between Bruner's three repersentational modes (enactive, iconic, symbolic) and the young children's prose learning. The main purpose of this study was to present the appropriate instruction media and method in young children's prose learning by investigating the effects of the repersentational modes, the area, and the kinds of word on young children's memory of key words in prose learning. And the other purpose was to give the basic information in selecting the words for instruction throuh the comparison of nouns and adjectives. The hypotheses of this study are as follows: Hypothesis 1. The young children win recall key words differently in ages. Hypothesis 2. The young children win recall key words better in the conditions of enactive representation and iconic representation than in the condition of symbolic representation, Hypothesis 3. The five, six, seven-year-olds will recall more nouns than adjectives. Hypothesis 4. There win be the interaction effect of the ages and the representational modes. Hypothesis 5. There win be the interaction effect of the representational modes and the kinds of word. For subjects in this study, a total of 90 children who know the written language and go to the kindergarten and the elementary school in Guang-Ju, Ma-San were randomly assigned to the three experimental conditone (actions, pictures, witten languages). The experimental instruments were the actions, the 10 pictures, and the 10 cards with the written languages, and the tapes with the prose passages by the experts. Stein's(1978) "Albert Gets Caught" was used for this study as in Jung Yeon-Suk'a paper(1986). The datas were colleted by the response test consisted of ten items that were divided to 0. 1. and 2 degrees by the responses. The collected dates were analized by the repeated 3×3×2 (Ages × Rrpesentational Modes × Kinds of Word) ANOVA. The following findings were obtained: First, the hypothesis 1 was accepted [F(2, 81) = 1.09, p<,01]. The seven-year-olds (the second grades) rememberd key words better then the five-year-olds and the six-year-olds. But the differonce between the memory of the five- year-olds and that of the six-year-olds was not. Second, the hypothesis 2 was accepted [F(2, 51) = 5.56, P<.01]. Namely the significant differences among the conditions of the notions, the pictures, and the written languages were. The means of the three conditions were 6.78, 6.98, 5.92. And be the mean of the iconic repersentation mods was highest. Third, the hypothesis 3 was sccepted [F(1,81) = 39.68. P<.01]. This indicates the main effect of the kinds of word. And this mians that the kinds of word affect the prose learning of the five, six, and seven-year-olds. Forth, the hypothesis 4 was rejected. the degrees in the three representational modes were consistent in disregard of ages. Fifth, the hypothesis 5 was accepted [F(2, 81) = 3.01, P<.10]. The young children remembered relatively well key words of adjectives in the iconic representation mode.

      • 환자검체에서 분리된 Staphylococcus와 Coagulase negative Staphylococcus의 항생제 감수성에 관한연구

        황구연 서강대학 지역발전연구소 1997 지역발전연구 : 서강전문대 Vol.4 No.1

        This study is an antibiotic sensitivity test that investigate Staphylococcus aureus 1,828 strains and coagulase negative Staphylococcus 2,139 strains about every year, isolated frequency, specimens and antibiotics from July 1994 to June 1997 in the specimen of genaral hospital patients. The results of this study are as follows : 1. Isolated frequency of each year that Staphylococcus aureus are almost the same three years and coagulase negative Staphylococcus increased 1 % every year. 2. Isolated frequency of each specimens that best specimen is wound after sputum, urine and blood in Staphylococcus aureus. 3. From the result of antibiotic sensitivity every year, we can see that Staphylococcus aureus shows resistance to Cephalothin, Clindamycin, Erythromycin, Oxacillin, Penicillin-G etc. but there was no difference every year. Coagulase negative Staphylococcus shows resistance very easily by using it widely. 4. The result of antibiotic sensitivity on each specimens generally shows little susceptibility except for Teicoplain and Vancomycin, but vaginal swab shows much susceptibility except for Penicillin-G. 5. Teicoplain shows 90 % susceptibility, Vancomycin shows 100 % susceptibility among all strains in Staphylococcus aureus and cogulase negative Staphylococcus.

      • 인삼이 생쥐에서 경구투여된 방사성스토론튬(Sr-85)의 배출에 미치는 효과

        황구연 서강정보대학 1993 論文集 Vol.12 No.-

        Effect of ginseng(6-year old white ginseng) on body absorption, fecal and urinary excretion of ingested radiostrontium(Sr-85) were investigated in the mouse (NIH-strain, male) treated with ginseng soluble fraction. The test groups were fed with basic diet supplemented with 1% each ginseng soluble fraction for 7 days before and after the radiostrontium administration by oral intubation. In the post-feeding groups of treated with ginseng soluble fraction, the radioactivities of fecal and urinary excretion and the amount of whole body retention were similier to control groups. In the pre-feeding groups of treated with ginseng soluble fraction, the radioactivities of fecal and urinary excretion were increase about 25% than that of control groups. And the amount of whole body retention were about 28%. In addition, the levels of accumulated radiostrontium retained significantly higher percent in skeletons than other organs.

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