가족성 근위축성측삭경화증을 유발시키는 두 번째 유전자 위치

홍성출 한국생명과학회 2001 생명과학회지 Vol.11 No.3

Amyotrophic lateral sclerosis(ALS) is a progressive neurologic disorder resulting from the degeneration of upper and lower motor neurons, and is inherited in 10% of cases. About 20% of familial ALS, clinically indistinguishable from sporadic ALS, is caused by mutations of Cu/Zn superoxide dismutase on chromosome 21q22.21 inherited as an autosomal dominant trait. We now report a new locus in the non-SOD1 dominantly inherited ALS. We screened a large ALS family with 11 affected individuals and one obligate gene carrier with genome-wide ABI polymorphic markers using the ABI 377 automated system. No evidence of linkage was obtained with the autosomal markers. We next screened this family with X chromosome markers as there was no evidence of male-to-male tran-smission of the disease. Linkage was established with several X chromosome markers with a lod score up to 3.8; almost the maximum possible score in this family. Our finding imply that a gene for the dominant expression of a neuronal degeneration is coded on X chromosome and raise the question of the role of X-linked genes that escape inactivation in this pathogenesis. More importantly, our finding that a gene causing ALS is localized on X-chromosome has direct investigational relevance to sporadic ALS, where epidemiological studies show male gender predominance(1.3:1) and earlier onset in men by 5-10 years.

대체혈액을 이용한 인공폐의 평가에 관한 연구

김기범,홍성출,김민호,정경락,이삼철,Kim K.B.,Hong S C.,Kim M.H.,Jheong G.R.,Lee S.C. 대한의용생체공학회 2000 의공학회지 Vol.21 No.3

본 연구에서는 새롭게 설계한 인공폐 모듈을 사용하여 인공폐의 효율을 평가하기 위한 압력손실과 산소전달에 대한 실험을 수행하였다. 또한 증류수, 아황산나트륨용액을 혈액대용물질로 사용하여 다른 인공폐의 성능을 평가하였다. 혈액대용물질은 압력손실과 산소운반력을 연구하는데 전혈(whole blood)에 비해 장점이 많다 즉. 이들은 상대적으로 값이 저렴하고 조절하여야 할 변수가 적다. 또한 이 용액을 사용하게 되면 탈산화장치가 필요 없으며 실험결과의 분석이 상대적으로 쉽다. 전혈과 같은 산소분압을 갖는 아황산나트륨용액을 사용하는 경우 아황산나트륨 용액의 산소전달속도는 전혈을 사용하는 경우의 산소전달속도와 거의 일치하였다. 이 실험에서 중공사모듈의 산소전달속도를 측정하는데 아황산나트륨용액이 혈액대용물질로 이용될 수 있음을 보였다. 이 인공폐 모듈에서는 용액이 중공사의 바깥쪽으로 흐르도록 설계하였는데 이 실험용 인공폐는 압력손실이 안쪽으로 흐르도록 하는 흐름에 비해 $1/3\~1/6$였다. In this paper a newly designed oxygenator module was used to perform the experiments for pressure drop and oxygen transport in order to evaluate the efficiency of the artificial lung. Also, distilled water. sodium sulfite solutions used in this experiment were evaluated for the performance of other artificial lungs. Substituted bloods have many advantages over whole blood in studying pressure drop and oxygen uptake. They are relatively inexpensive, and require fewer variables to be controlled. Furthermore, deoxygenation is not necessary when those solutions are used. In addition to these advantages. assays and interpretation of the experimental results are relatively easy. In the case of using the sodium sulfite solution having the same oxygen partial pressure as whole blood. the oxygen transfer rate of the sodium sulfite solution was basically same as that of whole blood. It was concluded in evaluating the function of artificial lungs that the sodium sulfite solution was suited for measuring oxygen transfer rate. In our module, artificial blood was flowed into the outside of hollow fiber membrane. The artificial lung created in this experiment showed that pressure drop was reduced to $1/3\~1/6$ of that compared to the inside-flow case.

부산 ·경남지역 노인환자의 질병양상

김공현,박형종,홍성출 인제대학교 1995 仁濟醫學 Vol.16 No.2

1992년 8월 26일에 실시한 보건복지부의 "환자조사" 자료를 이용하여 부산시와 경상남도에 소재한 의료기관에서 의료서비스를 받은 65세 이상 노인들의 질병양상을 분석하였다. 대상자는 입원환자의 경우 1992년 6월1일부터 6월 30일까지 퇴원한 환자 8,333명이었고 외래환자의 경우는 조사 당일의 환자 23,776명이었다, This study was carried out to idnetify diseases pattern of elders aged 65 years and over which may be used as basic data for developing measures to cope with health care problems of elders. The subjects of this study were 8,333 elders discharged from hospitals and clinics located in Pusan City and Kyungsangnam-do during June 1 to June 30, 1992, and 23,776 elders who visited the hospitals and clinics in Pusan City and Kyungsangnam-do for medical care on August 26, 1992. A file of data for this study was provided by the Ministry of Health and Welfare which conducted A National Patients Census in 1992. Diseases pattern were classified with three-digit categories of International Diseases Classification revised in 1975. The top ten common diseases of discharged elders were that the first cataract (4.70%), the second diabetes mellitus (2.54%), the third conscussion (2.53%), the fourth fracture of tibia and fibula (2.18%), the fifth cerebral laceration and contusion (2.16%), the sixth sprains and strains of other and unspecified parts of back (1.80%), the seventh essential hypertension (1.78%), the eighth contusion of low limb and of other unspecified sites (1.76%), the nineth acute appendicitis (1.58%), the tenth chronic airways obstruction, loot elsewhere classified (1.54%). The spells of these top ten common diseases were 22.6% of the total spells of the subjects. The top ten common diseases of outpatient elders were that the first gastritis and duo-denitis (4.02%), the second essential hypertension (3.14%), the third other and unspecified disorders of back (3.10%), the fourth diseases of hard tissues of teeth (3.04%), the fifth inflammatory disease of cervix, vagina and vulva (2.99%), the sixth diseases of pulp and periapical tissues (2.69%), the seventh acute nasopharyngitis (2.16%), the eighth sprains and strains of other and unspecified parts of back (2.01%), the nineth gingival and periodontal diseases (1.80%), the tenth contact dermatitis and other eczema (1.71%). The spells of these top ten common diseases were 26.7% of the total spells of the subjects. Considering diseases pattern of elders, it can be inferred that it is important for elders to take nutritionally balanced diet, to have healthy lifestyle, to practice physical excrcises and to organize effective health programs for the secondary prevention in order to live life long actively.

Iron‑dextran as a thermosensitizer in radiofrequency hyperthermia for cancer treatment

정해종,김현진,홍성출 한국응용생명화학회 2019 Applied Biological Chemistry (Appl Biol Chem) Vol.62 No.-

Radiofrequency hyperthermia is a recently rediscovered oncotherapy rising in popularity. However, lack of a proper thermosensitizer limits current radiofrequency hyperthermia to be only slightly effective, mostly being used as a subsidiary to a standard oncotherapy. Here, we report that iron-dextran delivers iron ion to cancer cells for cancer-selective accumulation of the iron ion, which functions as a thermosensitizer for radiofrequency hyperthermia. Intravenous injection of iron-dextran to tumor-xenografted mice resulted in selective accumulation of iron ion in the targeted cancer cells. The accumulated iron ion in cancer cells dramatically reacted to radiofrequency wave to result in tumorselective dielectric temperature increment without harming the surrounding normal tissue. The oncotherapeutic effect of was evaluated using tumor-xenografted mice. The overall anticancer efficacy of radiofrequency hyperthermia after injection of iron-dextran as a thermosensitizer in breast cancer-bearing mice was much better than the efficacy of paclitaxel, a standard chemotherapy drug for cancer. Moreover, hyperthermia using iron-dextran as a thermosensitizer completely eradicated cancer in the tumor xenografted mice. This work suggests that iron-dextran is an ideal thermosensitizer for radiofrequency hyperthermia. We believe that the application of iron-dextran as a thermosensitizer would be a major progress in hyperthermia cancer treatments.

대사성질환 예방을 위한 레스베라트롤 생합성 벼의 안전성 평가

임명호,백소현,홍성출,박순기,이시명,박종석,서석철,권순종 한국육종학회 2011 한국육종학회 학술발표회 발표요지 Vol.43 No.-

악관절에 발생한 색소성 융모결절성 활막염 1례

이국행,성명식,홍성출,정진행 대한이비인후과학회 2001 대한이비인후과학회지 두경부외과학 Vol.44 No.4

Early phase of amyloid β42-induced cytotoxicity in neuronal cells is associated with vacuole formation and enhancement of exocytosis

Meng-Lu Liu,홍성출 생화학분자생물학회 2005 Experimental and molecular medicine Vol.37 No.6

Amyloid β (Aβ) neurotoxicity is believed to play a critical role in the pathogenesis of Alzheimer's disease (AD) mainly because of its deposition in AD brain and its neuronal toxicity. However, there have been discrepancies in Aβ-induced cytotoxicity studies, depending on the assay methods. Com-β42-induced in vitro cyto-toxicity might be useful to elucidate the etiological role of Aβ in the pathogenesis of AD. In this study, MTT, CCK-8, calcein-AM/EthD-1 assays as well as thorough microscopic examinations were compara-tively performed after Aβ42 treatment in a neuronal precursor cells (NT2) and a somatic cells (EcR293). Extensive formation of vacuoles was observed at the very early stage of Aβ42 treatment in both cells. Early observation of Aβ42 toxicity as seen in vacuole formation was also shown in MTT assay, but not in CCK-8 and calcein-AM/EthD-1 assays. In addition, Aβ42 treatment dramatically accelerated MTT for-mazan exocytosis, implying its effect on the ex-tensive formation of cytoplasmic vacuoles. Aβ42 seems to cause indirect inhibition on the intracellular MTT reduction as well as vacuole formation and exocytosis enhancement. Following the acute cellular dysfunction induced by Aβ42, the prolonged treatment of micromolar concentration of Aβ42 resulted in slight inhibition on redox and esterase activity. The early Aβ42-induced vacuolated mor-phology and later chronic cytotoxic effect in neuronal cell might be linked to the chronic neurode-generation caused by the accumulation of Aβ42 in AD patients' brain.

A New Method for Transduction of Mesenchymal Stem Cells Using Mechanical Agitation

박진오,Sung-Hoon Park,홍성출 한국분자세포생물학회 2009 Molecules and cells Vol.28 No.6

Applications of bone marrow-derived mesenchymal stem cells in gene therapy have been hampered by the low effi-ciency of gene transfer to these cells. In current transduc-tion protocols, retrovirus particles with foreign genes make only limited contact with their target cells by passive diffusion and have short life spans, thereby limiting the chances of viral infection. We theorized that mechanically agitating the virus-containing cell suspensions would in-crease the movement of viruses and target cells, resulting in increase of contact between them. Application of our mechanical agitation for transduction process has in-creased the absorption of retrovirus particles more than five times compared to the previous static method without changing cell growth rate and viability. The addition of a mechanical agitation step increased transduction effi-ciency to 42%, higher than that of any other previously-known static transduction protocol.

두경부 암의 예후인자로서의 SUV 측정의 분석

이병철,심윤상,이용식,이국행,성낙윤,홍성출,김헌대,김병일,정광윤 대한이비인후과학회 2003 대한이비인후과학회지 두경부외과학 Vol.46 No.11

Background and Objectives:Previous studies have shown that uptake of 18Fluoro-2-deoxy-glucose in head and neck cancer, as determined by the standardized uptake value (SUV) on positron emission tomography scan (PET scan), is associated with the biology of tumor. The aims of this study were to confirm the association with the biology of tumor and to establish whether a high SUV had prognostic significance. Materials and Method:Thirty patients with the head and neck cancer diagnosed as squamous cell carcinoma underwent a PET scan before treatment. SUVs were analyzed for possibility correlated with disease-free survival. Results:In univariate survival analysis, when patients were divided into two groups based on the SUV cut-off (p= 0.029). Corelation analysis demonstrated that SUV provided prognostic information independent of the tumor size, pathologic differentiation and stage. Conclusion:We conclude that high FDG uptake on PET (SUV >8 in pre-treatment PET scan) is an important prognostic indicator for poor outcome. Identified patients are thought to require intensive treatment protocol and more careful follow up.(Korean J Otolaryngol 2003 ;46 :955-8)

AB42 세포독성 평가를 위한 MTT, CCK-8, Calcein-AM/EthD-1 분석법의 비교

유맹루 ( Meng Lu Liu ),유양 ( Yang Yu ),홍성출 ( Seong Tshool Hong ) 전북대학교 의과학연구소 2005 全北醫大論文集 Vol.29 No.1

Amyloid β peptide (Aβ) is believed to play a critical role in the pathogenesis of Alzheimer`s disease (AD). In this study we made a comparison of MTT, CCK-8 and Calcein-AM/EthD-1 assays for the viability of NT2 cells incubated with Aβ42, in order to provide basis for choosing the suitable method to detect Aβ42 toxicity, and clarify discrepancies about the potency of Aβ42 toxicity and the underlying mechanisms. In NT2 cells pre-incubated with nanomolar to micromolar Aβ42 for 0~20 hr, both enhanced MTT formazan exocytosis and decreased MTT reduction were observed, correlating to the extensive cytoplasmic vacuole formation. But the same treatment showed no inhibition on WST-8 (the major constituent in CCK-8 kit) reduction or on Calcein-AM hydrolysis, which meant no decline in cell proliferation or cell survival. Gradually increased inhibition on WST-8 reduction or on Calcein-AM hydrolysis was observed only when high concentration of Aβ42 and prolonged incubation time to 72 hr or longer were used. The results suggested that the formation of cytoplasmic vacuoles and the activation of endosomal/lysosomal system clearly preceded obvious cell injury and could be considered as an early sign of Aβ42 induced cellular dysfunction. The inhibition of the reduction system or the esterase activity was a comparatively late and serious event associated with a reduction in cell survival. Moreover, the enhanced MTT formazan exocytosis was directly related to the sensitive inhibition on MTT reduction by nanomolar Aβ42 in short time Compared to other methods, MTT assay was very sensitive for the early detection of Aβ42 cytotoxicity preceding obvious cell injury. For appropriate evaluation of cell injury or survival upon high concentration of Aβ42 and/or long incubation, combination of different methods is highly required.