http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
골에서 발생한 전이성편평세포암조직의 Acid Deoxyribonuclease의 활성과 작용기전에 관한 연구
현재요,김성준,고재경 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.2
Acid DNase in metastatic squamous cell carcinoma tissue of bone was partially purified, and substrate specificity of the enzyme and hydrolytic products of DNA by the enzyme were analyzed to investigate the mechanism of action of the acid DNase and tis role in carcinogemesis and maintenance of the bone cancer. Also studied were changes in patterns of proteins associated with the acid DNase analyzing proteins eluted with the enzyme by a DEAE-cellulose column chromatography, HPLC and PAGE. Concentrations of protein and nucleic acids were incresed significantly in metastatic squamous cell carcinoma tissue of the bone and acid DNase activity was markdely increased. The positive rate of the enzyme activity as a marker for the bone cancer was shown to be high, suggesting that the acid DNase could be used as a biochemical marker for metastatic squamous cell carcinoma. DEAE-cellulose column chromatographical analysis revealed that proteins in the metastatic squamous cell carcinoma tissue were separated into 7 peaks, of which peak Ⅶ appeared to be specific to the bone cancer. Acid DNase in the bone cancer tissue was eluted in a single peak along with protein peak Ⅱ as in the case with that of control bone tissue of the bone. Acid DNase in metastatic squamous cell carcinoma tissue was partially purified up to 44-fold by centrifugation, ultrafiltration, and DEAE-cellulose column chromatography. The partially purified enzyme was much more active against ds DNA than ss DNA, and species specificity of the hydrolytic products of ds DNA by the purified enzyme are found to be oligodeoxyribonucleotides, suggesting that the acid DNase is endonuclease in nature. HPLC and PAGE patterns for proteins associated with acid DNase (DEAE-peak Ⅱ proteins) from metastatic squamous cell carcinoma tissue appeared to be different from those from control bone tissue. This means that nature of proteins complexed with the acid DNase from the bone cancer would be different in nature from that from the control bone tissue, but does not mean that nature of the acid DNase from the bone cancer is different from that from control bone tissue. Although the acid DNase in metastatic squamous cell carcinoma tissue exhibited no absolute specificity toward ds DNA and the enzyme did not appear to be specific to the bone cancer, observations that the acid DNase activity in the bone cancer tissue was markedly increased and the enzyme was endonuclease in nature suggested a psssible role of the acid DNase in carcinogenesis and maintenance of the metastatic squamous cell carcinoma of the bone.