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Vibrio alginolyticus에 대한 특이 bacteriophage의 생물학적 특성
허용주 ( Yong Ju Heo ),이찬흔 ( Chan Heun Lee ),백민석 ( Min Suk Baek ),안현미 ( Hyun Mi Ahn ),황요셉 ( Yo Sep Hwang ),박권삼 ( Kwon Sam Park ),최상훈 ( Sang Hoon Choi ) 한국수산과학회 2012 한국수산과학회지 Vol.45 No.6
Vibrio alginolyticus, a marine f ish and shellf ish pathogen, has been found at a high frequency around the coastal areas of Korea. Vibrio alginolyticus was purif ied from various diseased f ish, and a V. alginolyticus-specif ic bacteriophage was isolated from seawater obtained from f ish farms located on the west coast of Korea. In a bacterial lysis experiment using the phage and antibiotics, tetracycline, 103 cfu/ml of V. alginolyticus were completely lysed by both the phage and the antibiotic, suggesting that the purif ied phage in the study could be utilized as an alternative to antibiotics in the control of f ish and shellf ish diseases caused by V. alginolyticus.
서해안 양식장에서 분리한 Vibrio alginolyticus의 특이 bacteriophage에 대한 구조적 특성
허용주 ( Yong Ju Heo ),이찬흔 ( Chan Heun Lee ),백민석 ( Min Suk Baek ),안현미 ( Hyun Mi Ahn ),황요셉 ( Yo Sep Hwang ),박관하 ( Kwan Ha Park ),최상훈 ( Sang Hoon Choi ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
Vibrio alginolyticus (V. alginolyticus), which is one of bacterial pathogens evoking severe infection in fish and shellfish as well as in human has been found at high frequency around all coast areas in Korea. Both V. alginolyticus and V. alginolyticus specific bacteriophage were isolated from sea water and various fishes from fish farms on west coast in Korea. In a morphological study based on electron microscope, the purified phage appeared to be composed of hexagon head of 60 nm and short tail of 20 nm. In the denatured SDS-PAGE analysis, the structural proteins of the phage were found to be 7 different protein fractions ranging from 37.8 to 198 kda. The kind of nucleic acid of the phage was ascertained to a double stranded DNA.
서해안 양식장에서 분리한 Edwadsiella tarda의 특이 bacteriophage 특성
이찬흔 ( Chan Heun Lee ),허용주 ( Yong Ju Heo ),백민석 ( Min Suk Baek ),이정은 ( Jung Uen Lee ),강자영 ( Ja Young Kang ),한미정 ( Mi Jung Han ),경서봉 ( Se Bong Kyoung ),최상훈 ( Sang Hoon Choi ) 한국어병학회 2011 한국어병학회지 Vol.24 No.2
Edwardsiella tarda (E. tarda)는 담수 및 해수어에 광범위하게 감염을 일으키는 그람 음성 박테리아이다. 본 연구에서는 서해안 넙치 양식장에서 분리한 E. tarda에 대해 특이 용균을 보이는 파아지를 분리하여 파아지의 활성에 미치는 환경적 요인인 pH와 배양온도의 영향 등을 조사하였다. 또한 어류모델로서는 담수어종인 Nile tilapia (Oreochromis niloticus)를 이용하였으며 E. tarda와 파아지와의 상호작용을 알아보았다. pH 안정성 실험에서는 담수보다 해수에서 높은 활성을 보였으며 온도 안정성 실험 결과 50℃까지 일정한 활성을 보였다. Tilapia 감염 실험에서는 파아지와 E. tarda를 접종하였다. 그 결과 실험군은 대조군에 비해 24시간째까지 E. tarda수가 일정하게 감소하는 것을 확인하였으며 파아지는 처음 접종 양보다 약간 증폭됨을 알 수 있었다. 본 연구의 결과는 E. tarda에 의해 발생되는 질병을 특이 파아지를 이용하여 제어할 수 있음을 암시해준다. Edwardsiella tarda is a broadly infectious agent against freshwater and seawater fishes. In the present study, E. tarda specific phage was isolated from fish farms on the west coast of Korea, and the effect of environmental factors such as pH and water temperature on the phage activity was investgated. As a fish model, Nile tilapia (Oreochromis niloticus) was used and the interaction between E. tarda and phage was investigated. The phage activity, in pH test, appeared even higher in seawater than freshwater and was evenly constant up to 50℃. The phage and E. tarda were inoculated in tilapia and the phage activity and E. tarda viability were checked on the time intervals. As a result, the number of E. tarda in the group of E. tarda plus phage was constantly reduced to 24 hr post-inoculation compared to the control group without phage, whereas the phage activity was slightly increased in the experiment group. The results suggest that it might be possible to use phage to control the fish disease caused by E. tarda.
정맥주사 시 입원환아가 경험하는 동통에 대한 환아, 어머니, 간호사의 지각
김신정 ( Shin Jeong Kim ),김성희 ( Sung Hee Kim ),유연엽 ( Yun Yup Yu ),허용주 ( Yong Ju Heo ),명지연 ( Ji Youn Myung ),고은정 ( Eun Jeong Koh ),김광희 ( Kwang Hee Kim ),박선영 ( Sun Young Park ),방문숙 ( Mun Suk Bang ),염순림 ( S 부모자녀건강학회 2006 부모자녀건강학회지 Vol.9 No.1
Purpose: The purpose of this study was to provide basic data for hospitalized children under the condition of painful procedure. Method: The data were collected from July 15th to Oct 30, 2003 from 68 hospitalized children suffer from acute disease, 68 mothers take care of children, 14 pediatric ward nurses. To evaluate the degree of pain perception of children, mothers & nurses, face pain rating scale and behavior characteristic were used. Result: The result were as follows. 1) The pain perception score of children, mothers & nurses. child perceived highest as the score of 3.60 2) The most frequent behavioral characteristic children were `crying(47.1%)` mothers were `soothing the child(54.4%)` and nurses were `support not to move(52.9%)` 3) The degree of pain perception of children, mothers and nurses according to subjects` general characteristics and painful procedure, there were stastical difference according to children`s age, mothers age and duration of painful procedure 4) There were significant correlation between the degree of pain perception and behavioral characteristics of children, mothers & nurses. Conclusion: Nurses should not underestimate children`s pain and need to provide knowledge and information to the child and their parents regarding painful procedure.