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허만일,이수진,이경필,김홍균,안상현,김단비,유래형,김준식,임정옥 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.5
BACKGROUND: Skin grafts are required in numerous clinical procedures, such as reconstruction after skin removal and correction of contracture or scarring after severe skin loss caused by burns, accidents, and trauma. The current standard for skin defect replacement procedures is the use of autologous skin grafts. However, donor-site tissue availability remains a major obstacle for the successful replacement of skin defects and often limits this option. The aim of this study is to effectively expand full thickness skin to clinically useful size using an automated skin reactor and evaluate auto grafting efficiency of the expanded skin using Yucatan female pigs. METHODS: We developed an automated bioreactor system with the functions of real-time monitoring and remotecontrol, optimization of grip, and induction of skin porosity for effective tissue expansion. We evaluated the morphological, ultra-structural, and mechanical properties of the expanded skin before and after expansion using histology, immunohistochemistry, and tensile testing. We further carried out in vivo grafting study using Yucatan pigs to investigate the feasibility of this method in clinical application. RESULTS: The results showed an average expansion rate of 180%. The histological findings indicated that external expansion stimulated cellular activity in the isolated skin and resulted in successful grafting to the transplanted site. Specifically, hyperplasia did not appear at the auto-grafted site, and grafted skin appeared similar to normal skin. Furthermore, mechanical stimuli resulted in an increase in COL1A2 expression in a suitable environment. CONCLUSIONS: These findings provided insight on the potential of this expansion system in promoting dermal extracellular matrix synthesis in vitro. Conclusively, this newly developed smart skin bioreactor enabled effective skin expansion ex vivo and successful grafting in vivo in a pig model.
허만일,안선희,김휘강,송윤정,최은창,안상현,최우성,허증수,임정옥 한국조직공학과 재생의학회 2016 조직공학과 재생의학 Vol.13 No.1
Full skin auto-grafts are required for reconstruction of skin burns and trauma scars. However, currently available clinical approaches such as sheet skin graft, mesh skin grafts, artificial skin graft, and in vivo skin expansion have limitations due to their potential danger for secondary damage and scar formation at the donor site, and discomfort during skin expansion. We developed an advanced bioreactor system and evaluated its function in skin expansion using porcine full skin. The reactor was designed as a pneumatic cylinder type, was programmed to adjust the pressure and the operating time. The system was composed of culture chamber unit, environmental control unit, and monitoring unit. Skins were expanded at 200 kPa pneumatic force and the expanded skins were analyzed by immunohistochemistry and histology. Furthermore we carried out auto-grafting experiment of the expanded skins in vivo using Yucatan pigs and skins were harvested and histologically analyzed after 8 weeks. The results showed that the bioreactor expanded skins to 160% in 4 hours. Histological analysis of the expanded skins revealed that epidermal cells and dermal fibroblasts were viable and remained integrity. The results of auto-grafting experiment indicated that fibrosis and scars were not detected in the grafted skins. This study demonstrates that the newly developed skin bioreactor enabled to obtain large sized full skin rapidly and successful grating.
LED 조명산업을 통한 융복합기술의 활성화 방안에 관한 연구
허만일(Man-Il Heo),유왕진(Wang-Jin Yoo) 한국조명·전기설비학회 2012 조명·전기설비학회논문지 Vol.26 No.4
By using LED optical devices and improving the efficiency of optical devices as well as lighting apparatus for humans, LED lighting industry puts the purpose of manufacturing the main products related with information, electric, large-sized LCD BLU, automobile, shipbuilding, agriculture, medical treatment, environment, and telecommunications, etc. Moreover, the main products and the industrial lighting products are manufactured with fusion, as the result, LED lighting industry provides various effects as well as sources of technology based on practical use of LED technology in order to develop the industry from the perspective of the lower price, the higher efficiency, the higher function, the deeper reliance. In the future, not only the development of new LED technology but also the fusion and combination of digital IT technologies using wire-wireless communication, sensor, controller, and artificial intelligence will be expected to create new industrial fields.
각막 상피 재건을 위한 콜라겐 겔 압축 공정 기반의 하이브리드 콜라겐 시트 제작
홍현준(Hyeonjun Hong),허만일(Man-Il Huh),김홍균(Hong-Kyun Kim),김동성(Dong Sung Kim) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.2015 No.11
Limbal stem cell deficiency is one of a critical corneal disease which is accompanied by recurrent epithelial defects, corneal vascularization and conjunctivalisation, resulting in a blindness. Many research groups tried to develop a tissueengineered substrate for carrying limbal epithelial cells to regenerate the corneal epithelium. However, in vitro construction of tissue-engineered cell carrier is limited in materials to satisfy both mechanically stable and biologically compatible properties. In this regard, this study firstly suggest hybrid collagen sheet and its fabrication process to overcome the drawback. The hybrid collagen sheet can be successfully developed through integrating reconstituted type I collagen and decellularized corneal lenticule based on adequate collagen compression system and process.